JOURNAL OF BASIC MICROBIOLOGY
·
2021
·Vol. 61
·期 5
·419-429
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IF 2.281
Jie Chen,Juan Wu,Luosheng Fan,Rong Jia
The study investigated the characteristics of aerobic degradation of tetrabromobisphenol A (TBBPA) by Irpex lacteus F17 ( I. lacteus F17) under four different cometabolic substrates (phenol, glucose, sodium pyruvate, and sodium citrate). The biodegradation of TBBPA by I. lacteus F17 could be enhanced via cometabolism, and glucose (8 g/L) was confirmed to be the optimum carbon source. For different initial solution pH ranging from 3.0 to 8.0, the results showed that I. lacteus F17 could be applied to biodegrade TBBPA in a wide pH range of 4.0–8.0, and the degradation rate could reach the maximum 75.31%, while the debromination rate reached the maximum 12.40% under pH 5.0. In addition, it has been confirmed that Mn 2+ (50 μmol/L) could promote the secretion of manganese peroxidase and TBBPA biodegradation efficiency. Seven intermediates were identified by gas chromatography–mass spectrometry analysis, and the possible degradation pathways were proposed, which indicated the biodegradation of TBBPA might be subjected to debromination, β -scission, hydroxylation, deprotonation, and oxidation reactions.
研究领域:
环境生物学
·
微生物学
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DOI
高影响因子
MICROBIOLOGICAL RESEARCH
·
2025
·
IF 6.9
Haolong Li,Tianmeng Guo,Ziyi Luo,Jingying Chen,Xuewen Xie,Golam Jalal Ahammed,Airong Liu,Shuangchen Chen
s Biocontrol fungi may exert antagonistic effects by emitting volatile organic compounds (VOCs), thus identifying fungal VOCs is crucial for understanding biocontrol mechanisms and developing novel biofungicides. In this study, we examined the antagonistic effect of Irpex lacteus LL210 against three major fungal pathogens: Botrytis cinerea (tomato gray mold), Fusarium oxysporum (cucumber wilt), and Alternaria alternata (pepper leaf spots) using in vitro assays. The results of both the dual-culture and dual-petri-dish methods showed that I. lacteus LL210 strongly inhibited the growth of B. cinerea , likely through the release of volatile compounds. SPME-GC-MS analysis of I. lacteus LL210 identified 770 volatile compounds, of which 26 key volatiles were screened on the basis of their relative odor activity values, peak areas and concentrations. Further evaluation using the dual-petri-dish method showed that compounds such as 2-Octen-1-ol, (E)-; Benzeneacetaldehyde; (E)-2-Octenal; Hexanal; (E)-2-Butenal; 5-Heptenal, 2,6-dimethyl-; 1-Octanol; 2,3-dihydro-Benzofuran; Diallyl Sulfur compounds exhibited significant inhibition of B. cinerea , suggesting their potential utility in the development of novel fungicides. We also tested their effects on plant growth and physiology and found that 1-octanol had minimal deleterious effects on tomato plants, as evidenced by growth and oxidative stress markers. This study systematically deciphered the VOCs profile of I. lacteus LL210, revealing critical mechanisms of pathogen inhibition through both direct inhibitory effects of VOCs and plant-mediated enhanced defense. These findings have driven the development of potential biocontrol agents based on VOCs, thereby providing sustainable solutions for crop disease management.
研究领域:
植物病理学
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微生物学
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农业科学
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DOI
PubMed
高影响因子
AMB Express
·
2018
·Vol. 8
·期 1
·1-16
·
IF 1.719
Duan Zihong,Shen Rui,Liu Binjie,Yao Mengwei,Jia Rong
Irpex lacteus F17 is well-known for its ability to degrade recalcitrant aromatic pollutants, which mainly results from the action of the manganese peroxidase (MnP) that it is able to produce. Recently, the genome sequencing and annotation of this strain provided comprehensive picture of the ligninolytic peroxidase gene family. In addition to revealing the presence of 13 MnPs, genes for five dye-decolorizing peroxidases (DyPs) were also discovered in the I. lacteus F17 genome, which are unrelated to the fungal class II peroxidases. In the present study, amino acid sequences of five DyPs and 13 MnPs, representing two different families of heme peroxidases, were analyzed. Of these, two enzymes, a DyP (Il-DyP4) and a MnP (Il-MnP6) were expressed respectively in Escherichia coli, and were characterized by comparing their molecular models, substrate specificities, and catalytic features. The results showed that Il-DyP4 possessed a higher catalytic efficiency for some representative substrates, and a stronger decolorizing ability to a wide range of synthetic dyes in acidic conditions. Based on electrochemical measurements, Il-DyP4 was found to have a high redox potential of 27 mV at pH 3.5, which was superior to that of Il-MnP6 (− 75 mV), thereby contributing to its ability to oxidize high redox potential substrates, such as veratryl alcohol and polymeric dye Poly R-478. The results highlighted the potential of Il-DyP4 for use in industrial and environmental applications.
研究领域:
环境生物学
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分子生物学
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酶学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Dongze Niu,Sainan Zhang,Xuanhe Chen,Chuangyang Xu,Yue Tang,Chunyu Li,Dongmin Yin,Taoli Huhe,Jianjun Ren
White rot fungi, such as Irpex lacteus , offer significant potential for lignocellulose degradation in sustainable biofuel and animal feed production, but optimizing nitrogen sources to balance fungal growth, lignin degradation, and protein synthesis remains a critical challenge. This study investigated the effects of ammonium chloride (NH₄Cl) and sodium nitrate (NaNO₃) on the solid-state fermentation of I. lacteus in wheat straw. While all treatments resulted in significant degradation of acid-insoluble lignin (AIL) compared to the raw material, only NH₄Cl supplementation increased fungal biomass. After 28 days of fermentation, NaNO₃ treatment achieved an absolute AIL loss ranging from 44.5 % to 61.4 %. Critically, the NH₄Cl group exhibited a significantly higher final AIL content than both the control and NaNO₃ groups ( P < 0.05). Furthermore, NaNO₃ enhanced glucose and xylose yields from the residual wheat straw by up to 19.8 % and 46 %, respectively, compared to the control. Transcriptomic analysis revealed that the NaNO₃ group had higher expression of genes related to nitrogen uptake and cellulose/hemicellulose degradation than the control. Compared to NH₄Cl, NaNO₃ also mitigated the downregulation of genes encoding manganese peroxidase and several cytochrome P450s. These findings suggest that NaNO₃ promotes I. lacteus metabolism by coordinating lignocellulose degradation with efficient nitrogen assimilation.
研究领域:
微生物学
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环境生物技术
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生物质能
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真菌生理学
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分子生物学
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代谢工程
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Peng Zhu,Dongze Niu,Sainan Zhang,Chunyu Li,Dongmin Yin,Junqiang Zhi,Lili Zhang,Xingmei Jiang,Jianjun Ren
This study aimed to examine the effects of sterilization methods on the degradation ability and bioactive compound production of Irpex lacteus in wheat straw. Following 28 days of fermentation, the lignin content of samples autoclaved and pasteurized at pH 4.5 was reduced by 16.0 % − 21.7 % compared to pasteurized samples without pH adjustment, accompanied by a significant increase in sugar yield ranging from 83.30 % − 96.35 %. Autoclaved samples exhibited the lowest total phenol content and antioxidant activity ( P < 0.05). Bacillus occupied an absolute advantage (89.1 %) in samples pasteurized at pH 4.5, whereas 10 bacterial genera exhibited abundances above 1 % in pasteurized samples without pH adjustment. Furthermore, 45.1 % − 47.2 % of the metabolites comprised lipids and lipid-like molecules, and some of them were improved by pasteurization at pH 4.5. Overall, pasteurization at acidic conditions is an effective sterilization method for the fungal conversion of wheat straw.
研究领域:
生物技术
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微生物学
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生物化学
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农业科学
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2016
·Vol. 23
·期 10
·9585-9597
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IF 2.76
Yang Xueting,Zheng Jinzhao,Lu Yongming,Jia Rong
Malachite green (MG), a recalcitrant, carcinogenic, and mutagenic triphenylmethane dye, was decolorized and detoxified using crude manganese peroxidase (MnP) prepared from the white rot fungus Irpex lacteus F17. In this study, the key factors (pH, temperature, MG, Mn 2+ , H 2 O 2 , MnP) in these processes were investigated. Under optimal conditions, 96 % of 200 mg L −1 of MG was decolorized when 66.32 U L −1 of MnP was added for 1 h. The K
m , V
max , and k
cat values were 109.9 μmol L −1 , 152.8 μmol L −1 min −1 , and 44.5 s −1 , respectively. The decolorization of MG by MnP followed first-order reaction kinetics with a kinetic rate constant of 0.0129 h −1 . UV–vis and UPLC analysis revealed degradation of MG. Furthermore, seven different intermediates formed during the MnP treatment of 0.5 h were identified by LC-TOF-MS. These degradation products were generated via two different routes by either N -demethylation of MG or the oxidative cleavage of the C–C double bond in MG. Based on ecotoxicity analyses performed on bacteria and algae, it was confirmed that MG metabolites produced by the MnP-catalyzed system were appreciably less toxic than the parent compound. These studies indicate the potential use of this enzyme system in the clean-up of aquatic and terrestrial environments.
研究领域:
环境科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
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