NEUROMOLECULAR MEDICINE
·
2022
·Vol. 25
·期 1
·53-63
·
IF 4.103
Cheng Jing,Fan Yan-Qin,Zhang Wen-Fei,Zhang Guo,Zeng Kuo,Ye Zhang,Zhao Dan,Wu Li-Quan,Chen Zhi-Biao
Sirtuin-6 (SIRT6), a member of the sirtuins family of NAD ( +) dependent deacetylases, has been shown to have beneficial effects in ischemic stroke. However, the role of SIRT6 in intracerebral haemorrhage (ICH) has not reported. We observed that SIRT6 expression was down-regulated in human ICH patients and down-regulated in ICH-induced rat cortical neurons. We subsequently found that SIRT6 overexpression reduced brain tissue damage and increased neuronal survival in the ICH model of rats and hemin-induced cortical neurons. Our further study found that overexpression of SIRT6 can reduce inflammatory response by down-regulating the expression of NF-kB and thus promote the recovery of neurological function in ICH animals. In conclusion, SIRT6 can inhibit the expression of NF-kB and plays a neuroprotective role in ICH by inhibiting the NF-kB-mediated inflammatory response.SIRT6 could be a novel therapeutic target for ICH.
研究领域:
神经科学
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ChemElectroChem
·
2019
·Vol. 6
·期 15
·3959-3965
·
IF 3.975
Min Liu,Houcai Dong,Shuo Zhang,Xi Chen,Yiping Sun,Shan Gao,Jiaqiang Xu,Xiaodong Wu,Anbao Yuan,Wei Lu
Graphical Powerful microspheres : A spherical nano-TiNb 2 O 7 /CNT-KB composite material with a 3D hierarchical heterostructure fabricated by spray drying and solvothermal method is presented. The material is characterized as an anode for lithium-ion batteries demonstrating superior electrochemical performance in terms of reversible charge specific capacity, high rate, and long-term cycling performance compared to bare TiNb 2 O 7 . Nano-TiNb 2 O 7 /multiwalled carbon nanotube and ketjen black (TiNb 2 O 7 /CNT-KB) composite microspheres with porous three-dimensional (3D) hierarchical heterostructure are fabricated by spray drying and solvothermal method. This composite material exhibits a high reversible charge specific capacity of 327.8 mAh g −1 at the current rate of 0.1 C and 151.1 mAh g −1 at 20 C rate. When it is cycled at a high current rate of 5 C for 1000 cycles, the charge capacity is dropped from the maximum value of 220.8 mAh g −1 (6 th cycle) to the end value of 145.4 mAh g −1 , with capacity retention of 65.9 %. The TiNb 2 O 7 /CNT-KB composite material demonstrates much superior electrochemical performance over the bare TiNb 2 O 7 material, and this result should be attributed to its particular porous 3D hierarchical structure, which possesses enhanced electrical conductivity as well as the ability for accommodating volume change and preventing particle aggregation of the electrode active material during the discharge and charge cycles.
研究领域:
材料科学
·
能源存储与转换
✓ 已验证
DOI
高影响因子
Materials Today Communications
·
2024
·
IF 3.7
Yuqi Wang,Wei Zhao,Mingpu Li,Qing Zhuo,Yuanyuan Li,Linli Tan,Yingru Li,Hangyu Dong,Qiong Long
Utilizing foam-structured composite materials for electromagnetic shielding offers several advantages, including lightweight properties, low filler load, and a high absorption coefficient. However, their application in electromagnetic shielding is often limited by relatively lower mechanical performance. This study employed melt blending and chemical foaming processes to fabricate a lightweight EPDM/POE/KB composite foam with favorable mechanical characteristics, heat resistance, and electromagnetic absorbing performance. Significantly, the foam, with a density of 0.195 g/cm³, demonstrates an absorption-dominated shielding mechanism (absorption coefficient of 0.71) and good electromagnetic shielding performance of 20.57 dB in the X-band. The honeycomb structure formed within the material contributes to the multiple reflections of electromagnetic waves. Additionally, the composite foam exhibits excellent flexibility, with a fracture elongation greater than 150 %, alongside tensile and compressive strengths of 1.46 MPa and 0.92 MPa, respectively, demonstrating satisfactory mechanical performance. Furthermore, the composite foam also possesses good thermal stability. The study provides a low-cost and effective method for preparing lightweight, high-absorption coefficient, and excellent mechanical performance electromagnetic shielding foam.
研究领域:
材料科学
·
电磁学
✓ 已验证
产品关联
DOI
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2023
·
IF 4.3
Eun-Ji Han,Eun-Young Choi,Su-Ji Jeon,Sang-Woo Lee,Jun-Mo Moon,Soo-Hyun Jung,Bumseok Kim,Sung-Dae Cho,Jeong-Seok Nam,Changsun Choi,Jeong-Hwan Che,Ji-Youn Jung
Natural products are continuously being researched to develop safe and effective treatment options for cervical cancer , the fourth most common cancer in women. Piperlongumine (PL), an amide alkaloid mainly present in long pepper , exhibits neuroprotective and anti-cancer properties. However, the specific effect of PL in cervical cancer and the relationship between the anti-cancer pathway and autophagy remain unclear. Therefore, we aimed to investigate PL-induced apoptosis in KB human cervical cancer cells and the relationship between apoptosis and autophagy therein. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and wound-healing assays showed that PL treatment suppressed KB cell viability and proliferation. Apoptosis was identified through 4′,6-diamidino-2-phenylindole and annexin V-propidium iodide staining, increased cleaved-poly (ADP-ribose) polymerase and Bcl-2 associated X levels, and decreased B cell lymphoma 2 levels. Acridine orange staining and increased microtubule-associated protein 1A/1B-light chain 3-II and Beclin-1 levels confirmed autophagy. We determined that KB cell-related autophagy exerted cytoprotective effects using the autophagy inhibitors 3-methyladenine and hydroxychloroquine . PL treatment promoted apoptosis by inhibiting the phosphatidylinositol-3-kinase (PI3K)/protein kinase B/mammalian target of rapamycin pathway in KB cells; inhibiting the pathway using PI3K inhibitors increased autophagy. We suggest that PL is a potential natural anticancer agent for cervical cancer treatment.
研究领域:
肿瘤学
·
药理学
·
细胞生物学
·
天然产物化学
✓ 已验证
DOI
PubMed
高影响因子
ChemPlusChem
·
2024
·
IF 3
Zhijun Zhu,Zhihong Yu,Guihuan Chen,Boyan Li,Aiju Li
Graphical The shuttle effect in lithium-sulfur batteries has been a thorny issue, for which researchers have conducted a lot of studies. Of the various directions attempted, separator modification is more convenient and easy to operate. In this paper, CeVO 4 /KB material is proposed to improve the separator and suppress the shuttle effect through its catalysis and adsorption. Catalytic adsorption of the rare earth metal Ce and transition metal V, efficient binding of oxides to polysulfides, and physical adsorption of porous KB prevent the transport of polysulfides to the anode and facilitate the reaction kinetics. Of course, the CeVO 4 /KB/PP batteries show brilliant performances. The CeVO 4 /KB/PP cell achieves 1059.9 mAh g −1 and 92.3 % capacity recovery rate when 3 C is reverted to 0.2 C, demonstrating the outstanding reversibility provided by the CeVO 4 /KB/PP separator. In the long cycle test, the initial specific capacity reaches 1200.9 mAh g −1 with a capacity retention rate of 86.5 % after 100 cycles at 0.2 C. Even at 3 C, CeVO 4 /KB/PP batteries reach 882.7 mAh g −1 in the first cycle with a capacity decay rate of 0.063 % per cycle on 1000 stable cycles. This work provides guidance on the selection of future separator modification materials and expects other work to be a breakthrough. Lithium-sulfur (Li−S) batteries display promise as redox-based batteries, where separators are an essential part of preventing short-circuiting of the positive and negative electrodes, while the shuttle effect is a critical issue of separators. Currently, commercial PP separators are weak in inhibiting the polysulfides shuttling, so modified separators are needed to inhibit it to improve the battery performance. This paper reports that CeVO 4 /KB composites act as separator materials. CeVO 4 /KB modified PP separators enhanced the adsorption of LiPSs, accelerated the rate of Li + migration, and catalyzed the conversion of LiPSs. These bring about the effect that CeVO 4 /KB/PP batteries reach 1200.9 mAh g −1 in the first cycle with a capacity retention rate of 86.5 % after 100 cycles at 0.2 C and reach 882.7 mAh g −1 of the initial cycle with a capacity decay rate of 0.063 % after 1000 cycles at 3 C. This work introduces rare earth metal vanadates to modify the separator, adding new ideas for designing separators for good-performance batteries.
研究领域:
材料科学
·
电化学
·
能源存储
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2021
·
IF 12.479
Shaojun Pan,Yuhui Zhang,Mark Huang,Zhoufeng Deng,Amin Zhang,Lijia Pei,Lirui Wang,Weiyong Zhao,Lijun Ma,Qian Zhang,Daxiang Cui
s Multi-functional nanovectors based on exosomes from cancer cell culture supernatants in vitro has been successfully utilized for tumor-specific targeting and immune escape. However, the labor-intensive purification procedures for rich-dose and high-purity homogeneous exosomes without using targeting ligands are still a challenging task. Herein, we developed a nanovector Exo-PMA/ [email protected] through cloaked by urinary exosome membrane as a chemo/chemodynamic theranostic nano-platform for targeted homologous prostate cancer therapy which pertain to the abrogation of Epidermal Growth Factor Receptor (EGFR) and its downstream AKT/NF-kB/IkB signaling instead of ERK signaling cascades. Urinary exosomes-based nanovectors own the same urological cancer cell membrane antigen inclusive of E-cadherin, CD 47 and are free from intracellular substance such as Histone 3 and COX Ⅳ. The targeting properties of the homologous cancer cell are well preserved in Exo-PMA/ [email protected] nanovectors in high purity. Meanwhile, the nanovectors based on urinary exosomes from prostate patients deeply penetrated into prostate cancer DU145 3D MCTS , and successfully achieve superior synergistic low-dose chemo/chemodynamic performance in vivo . In addition, the blockage of bypassing EGFR/AKT/NF-kB/IkB signaling pathway is greatly enhanced via elevated intracellular PMA/ [email protected] nanoparticles (NPs). It is expected that the rich source and high purity of urinary exosomes offer a reliable solution for mass production of such nanovectors in the future. The targeted homologous cancer therapy based on the urinary exosomes from cancer patients exemplifies a novel targeted anticancer scheme with efficient and facile method.
研究领域:
癌症生物学
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纳米技术
·
药学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2017
·
IF 8.402
Qin Wang,Hao Jiang,Yan Li,Wenfei Chen,Hanmei Li,Ke Peng,Zhirong Zhang,Xun Sun
The transcription factor NF-kB plays a pivotal role in the pathogenesis of rheumatoid arthritis. Here we attempt to slow arthritis progression by co-delivering the glucocorticoid dexamethasone (Dex) and small-interfering RNA targeting NF-kB p65 using our previously developed polymeric hybrid micelle system. These micelles contain two similar amphiphilic copolymers : polycaprolactone-polyethylenimine (PCL-PEI) and polycaprolactone-polyethyleneglycol (PCL-PEG). The hybrid micelles loaded with Dex and siRNA effectively inhibited NF-kB signaling in murine macrophages more efficiently than micelles containing either Dex or siRNA on their own. In addition, the co-delivery system was able to switch macrophages from the M1 to M2 state. Injecting hybrid micelles containing Dex and siRNA into mice with collagen-induced arthritis led the therapeutic agents to accumulate in inflamed joints and reduce inflammation, without damaging renal or liver function. Thus, blocking NF-kB activation in inflammatory tissue using micelle-based co-delivery may provide a new approach for treating inflammatory disease.
研究领域:
药理学
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纳米技术
·
风湿病学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Maolin Li,Yuting Zhang,Xiuyuan Jiang,Jiao Jiang,Xina Gu,Hua Zhang
Transcription factors play crucial roles in governing gene expression and are implicated in various physiological and pathological processes. Here, we propose a novel electrochemiluminescence (ECL) biosensor for the sensitive and specific detection of the transcription factor NF-kB p50, utilizing CRISPR/Cas12a-assisted hyperbranched rolling circle amplification (HRCA) technology. The biosensor achieves a remarkable limit of detection (LOD) of 3.6 fM and demonstrates excellent specificity against interfering proteins. Optimization of experimental parameters further enhances the biosensor’s performance, ensuring its reliability and accuracy. In addition, validation in real biological samples confirms the feasibility of biosensor for practical applications, offering a promising tool for clinical trials and disease diagnosis.
研究领域:
生物技术
·
分子生物学
·
分析化学
·
临床诊断
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Yajing Fang,Mengmeng Xia,Fuqiang Liang,Weiwei Cao,Siyi Pan,Xiaoyun Xu
This study was aimed to determine the mechanism for flavonoid poor absorption related to P-glycoprotein (P-gp). The cellular uptake (CU) of 40 flavonoids was investigated in P-gp overexpressing KB/multidrug-resistant (MDR) cells. A total of 9 flavonoids, including 5,7,3′,4′-tetramethoxyflavone, with a significant (p < 0.05) CUKBE (2.90 ± 0.146 μmol/g) higher than CUKBP (1.57 ± 0.129 μmol/g) were identified as P-gp substrates. Besides, 8 substrates, including tangeretin, showed a significant (p < 0.05) CUKB (9.72 ± 1.09 μmol/g) higher than its CUKBP (7.36 ± 0.692 μmol/g). A total of 7 of 17 flavonoid substrates stimulated the P-gp efflux of rhodamine 123, and most substrates increased P-gp expression in KB/MDR cells. Docking analyses showed a good correlation (R = 0.764; p < 0.01) between efflux fold and S_scoring of flavonoids to the P-gp model, indicating consistency between in silico and in vitro results. A structure–affinity relationship exhibited that 3-OH, 5-OH, 3′-OCH3, and 4′-OCH3 are crucial for flavonoids binding to P-gp. These results provide valuable information for finding a solution to improve the absorption of flavonoids.
研究领域:
药理学
·
癌症研究
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF ARTHROPLASTY
·
2024
·
IF 3.4
Junqi Niu,Fanggang Bi,Qing Tian,Ke Tian
Background Aseptic loosening around the prosthesis is a common cause of failure in total joint arthroplasty. Polyethylene wear particles trigger the release of inflammatory factors by macrophages. Key mediators involved in osteoclastogenesis include interleukin-6, tumor necrosis factor-α, receptor activator of nuclear factor kappa B (RANK), RANK ligand (RANKL), and bone protection hormone (Osteoprotegerin [OPG]). The purpose of our experiment was to see whether melittin can slow down the release of inflammatory mediators through the NF-kB pathway, regulate the RANKL/OPG ratio, reduce osteoclast formation, and delay the onset of arthritis in rats. Methods A total of 20 male Sprague-Dawley rats (10 months, Specific Pathogen Free, 350 g ± 20 g) were randomly divided into 5 groups: sham group, model group, melittin concentration 1 group (0.2 mg/kg), concentration 2 group (0.4 mg/kg), and concentration 3 group (0.6 mg/kg). All rats were implanted with TA2 high-purity titanium rods. A drill was used to create a bone canal along the long axis of the femur in the intercondylar notch. The model group and experimental groups were exposed to polyethylene particles, while the sham group did not receive any particles. Results The melittin group exhibited significantly increased serum levels of serum P, calcium-phosphorus product, OPG, PINP, PINP/CTX-I, and OPG/RANKKL ( P < .05). In the experimental group, micro computed tomography scanning results revealed a decrease in the amount of bone defect around the prosthesis. Immunofluorescence analysis demonstrated a decrease in the expression of IKKα and P65, while the expression of OPG showed an upward trend. Both Hematoxylin-Eosin and Tartrate-Resistant Acid Phosphatase staining revealed less osteoclast and inflammatory cell infiltration in bone resorption pits. Conclusions Our study demonstrates that melittin has the ability to inhibit the NF-kB pathway in a rat model, and reduce the impact of RANKL/OPG, thereby delaying osteoclast activity and alleviating periprosthetic osteolysis.
研究领域:
骨科
·
免疫学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2021
·
IF 6.025
Yajing Fang,Fuqiang Liang,Mengmeng Xia,Weiwei Cao,Siyi Pan,Ting Wu,Xiaoyun Xu
This study was aimed to investigate the inhibitory activity of flavonoids on P-glycoprotein (P-gp). Effects of 39 flavonoids on the cellular uptake (CU) of rhodamine123 (Rho) and daunomycin (DNR) were investigated in both parental KB and P-gp overexpressed KB/MDR cells. The inhibition mechanism of selected flavonoids was further investigated by measuring the ATPase activity and expression level of P-gp. Twelve flavonoids improved the uptake of Rho (↑Rho F ) and nineteen flavonoids increased the uptake of DNR (↑DNR F ) in KB/MDR cells with nine flavonoids overlapped. Structure-activity relationship (SAR) indicated that 8-OCH 3 , and 2′-OH have a negative effect on Rho and DNR transport. Whereas 5-OH, 5-OCH 3 , 6-OH, 7-OCH 3 , 3′-OH, and 4′-OH, are essential for inhibition of flavonoids on P-gp and reversing the resistance of Rho and DNR. Eleven selected flavonoids significantly induced the basal P-gp-ATPase activity but much lower than that induced by verapamil . Tangeretin , galangin , kaempferol , quercetin , and morin significantly reversed the ATPase activity stimulated by verapamil. Six of eleven flavonoids significantly decreased P-gp expression, whereas three flavonoids slightly increased P-gp expression. These results provide valuable information that flavonoids can effectively reverse multidrug resistance of P-gp-mediated transport of nutraceutical and drugs by co-administration.
研究领域:
药理学
·
肿瘤学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Bioscience-Landmark
·
2019
·Vol. 24
·期 5
·833-848
·
IF 2.214
Gui-xin Zhang,Chen Zhan,Kai Wang,Jian Han,Dong Shang,Hai-long Chen
Acute biliary pancreatitis (ABP) is a potentially life-threatening disease that is induced by the common bile duct (CBD) sludge or stones. This study aimed to investigate protective effects of Qingyi Decoction (QYT) on deoxycholic-acid-sodium salt (DCA) induced ABP in rats. Gpbar1 is a G-protein coupled receptor that can be activated by DCA. Both Gpbar1 overexpression vector and Gpbar1 RNAi were constructed and transfected into ABP cell models. Functional assays reveal that DCA significantly induced AR42J apoptosis and triggered Gpbar1 expression. Gpbar1 significantly activated caspase 8 and caspase 9 as compared to LV5-NC and LV3-NC (p<0.05). Gpbar1 significantly triggered apoptosis associated inflammatory factors as compared to LV5-NC and LV3-NC (p<0.05). Gpbar1 significantly induced calcium flux as compared to LV5-NC and LV3-NC (p<0.05). Gpbar1 up-regulated caspases and inflammatory factors in DCA treated pancreatic acinar cells. QYT reversed DCA induced apoptosis and inflammatory response. QYT significantly reduced Gpbar1 levels compared to no-QTY treated cells (p<0.05). In conclusion, QYT protects against DCA induced pancreatic acinar cell damage in ABP by inhibiting Gpbar1/NF-kB/p-RIP signaling pathway.
研究领域:
药理学
·
胃肠病学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）