Bioengineered
·
2022
·
IF 6.832
Wenjun Jiang,Xiaoying Wang,Siwei Su,Sen Du,Hongqiang Song
Osteoarthritis (OA) is a common chronic degenerative disease characterized by the loss of articular cartilage, which causes loss of joint function and reduce quality of life. Resolvin D1 (RvD1) has shown interesting anti-inflammatory effects; however, the mechanism of action of RvD1 in OA remains unclear. The aim of this study was to investigate the potential mechanism of RvD1 in OA by bioinformatics and partial in vitro mechanisms. Here, 106 shared differentially expressed genes (DEGs) were identified based on the GSE82107, GSE55235, GSE55457 dataset; 700 DEGs were identified based on GSE169077. Enrichment analyses of these genes were then successively conducted. RvD1-targeted genes and KEGG pathways are identified by STITCH. 27 shared KEGG pathways were identified among RvD1-targeted pathways and OA. Furthermore, cell apoptosis assay, western blotting, real-time fluorescent quantitative PCR (qRT-PCR), enzyme linked immunosorbent assay (ELISA) were used to confirm the expression levels of the key genes of shared Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways between RvD1-targeted and OA in IL-1β treated rat knee chondrocytes. The results showed that RvD1-targeted pathways and the expression of nuclear p65, p53, and p-JNK were inhibited in the RvD1 group compared with the IL-1β group. Thus, the findings indicate that RvD1 may inhibit the development of OA through NF/kB, p53, MAPK/JNK, PI3K-AKT signaling pathways, and act as a treatment for OA.
研究领域:
生物信息学
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分子生物学
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药理学
·
骨科
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Zhenqian Wei,Weiyan Zhang,Menghao Du,Haiyan Zhong,Xuezhi Fang
Camellia saponins are important by-products of Camellia Oleifer Abel. processing. In this study, an eco-friendly method based on natural deep eutectic solvents (NaDESs, proline and glycerol at a molar ratio of 2:5) was established to extract saponin s from C.oleifera cakes. The content of saponin (702.22 ± 1.28 mg/g) obtained using NaDES was higher than those extracted using water or methanol. UPLC-Q-TOF MS analysis of chemical structure showed that the difference in the extraction technique alter individual saponins. A widely targeted metabolomic approach and KEGG metabolic pathway analysis showed that the upregulated metabolites in the NaDES-based extract mainly included flavonoids, alkaloids, and phenolic acids; and they were involved in arginine and proline metabolism, metabolic pathways, phenylpropanoid biosynthesis, biosynthesis of secondary metabolites, and flavonoid biosynthesis. The present study proposes a selective substitute for use in the extraction of camellia saponins with composition analysis.
研究领域:
植物化学
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代谢组学
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药用植物研究
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Zhuozhen Li,Xin Shu,Yicheng Chen,Yuyang Guo,Wenjie Yu,Liangliang Qu,Qiuning Wang,Yifan Zhang,Fangjian Ning,Jinwang Li,Tao Liu,Liping Luo
Thinned young citrus fruits (TYCF), an underutilized agricultural byproduct, contain abundant bioactive phenolic and flavonoid compounds. However, efficient extraction is limited by the environmental and safety issues of conventional solvents. Thus, green and effective methods are increasingly pursued to recover bioactives from citrus byproducts. This study investigated the extraction of bioactive compounds from TYCF using ultrasonic-assisted extraction (UAE) coupled with natural deep eutectic solvents (NADES). The results showed that the NADES system containing choline chloride and glycerol (ChCl-Gly) achieved the highest extraction efficiency under UAE conditions. Metabolomic profiling coupled with Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that the NADES-based extract exhibited upregulated metabolites, particularly flavonoids, organic acids, phenolic acids, and amino acid derivatives, which were predominantly involved in flavonoid, phenylpropanoid, and flavone and flavonol biosynthesis pathways. FTIR, SEM, and XRD analyses demonstrated that NADES extraction more effectively disrupted cell structures and reduced cellulose crystallinity compared to traditional solvents, resulting in higher yields of bioactive compounds from TYCF. This study highlights the potential of NADES as a green and efficient alternative for valorizing agricultural byproducts.
研究领域:
食品科学
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分析化学
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植物生物化学
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代谢组学
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绿色化学
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产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
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2018
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IF 3.47
Xinyue Wu,Lijuan Wang,Lei Tang,Li Wang,Shuyuan Cao,Qian Wu,Zhan Zhang,Lei Li
Consumption of Salvia miltiorrhiza could improve dextran sulfate sodium (DSS) induced colitis. Whether salvianolic acid B (Sal B) can ameliorate colitis is still unclear. Here, colitis induced by 2.2% DSS was treated with 100 mg/kg Sal B by oral gavage. Sal B improved weight loss, disease activity index, mucosal damage and inflammatory cell infiltration in colitis mice. Sal B could increase the production of acetic acid, propionic acid and valeric acid in colitis mice. The reduction of diversity and richness of gut microbiota in colitis mice was restored after Sal B treatment. Sal B reversed the decrease of S24-7 and the increase of Bacteroides in DSS-treated mice. There were 3 KEGG pathways related to enzyme families, cancer and lipid metabolism enriched in mice colitis after Sal B treatment. In conclusion, the beneficial aspects of dietary Sal B upon colitis are associated with microbial habitat.
研究领域:
药理学
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微生物学
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免疫学
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产品关联
DOI
高影响因子
Natural Product Communications
·
2024
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IF 1.5
Zhang-lin Pu,Chi Zhang,Qian Yan,Zhou Liang,Zhi-Wei Xu,Ji-hu Wei,Hua Liu,Feng Chen
Objective: We explore the pharmacodynamic targets of kaempferol in treating osteoporosis using transcriptomics and animal experiment. Methods: Firstly, we constructed an ovariectomized rat model (OVX). The anti-osteoporosis effect of kaempferol was evaluated by bone mineral density (BMD) and hematoxylin-eosin staining comprehensively. Moreover, differential genes between groups were screened by RNA sequencing technology (RNA-seq) for transcriptomics and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways enrichment analysis were performed. Finally, partial results of transcriptomics were verified to detect the expression of the relevant gene expression by immunohistochemistry. Results: The pharmacodynamic findings indicated that the administration of kaempferol resulted in an elevation in BMD (P < .01) and a notable enhancement in tibia microstructural indices in the experimental rats (P < .01). The transcriptomic analysis revealed that NTN1, LTBP4, GSN, and EBF1 were identified as the principal targets for therapeutic intervention in osteoporosis. The results of animal experiments showed that kaempferol promoted osteogenesis and inhibited bone resorption by downregulating the protein expression of NTN1, LTBP4, GSN, and EBF1 (P < .01). Conclusion: Kaempferol enhances BMD levels, retards tibial bone loss with structural deterioration in OVX model rats, and promotes bone formation while curbing bone resorption through NTN1, LTBP4, GSN, and EBF1 protein down-regulation.
研究领域:
药理学
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分子生物学
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骨科医学
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DOI
高影响因子
Metabolites
·
2025
·Vol. 15
·期 3
·152
·
IF 3.7
Yangzong Zhuoma,Minghong Yang,Yijie Chen,Xiangxi Zhang,Xingyan Duan,Hongwei Cui,Xin Fang,Xujia Hu
Background:Wild boletes are famous for their exceptional flavor and nutritional value. Due to their susceptibility to decay and spoilage, dry storage is a common method for processing and preservation. However, few studies have reported on the alterations of metabolites of boletes resulting from different drying methods. This paper aims to investigate the metabolic changes in two species of boletes,Butyriboletus roseoflavusandLanmaoa asiatica, subjected to three drying methods: hot-air drying, microwave drying, and freeze drying.Method and Result:Nuclear magnetic resonance (NMR) metabolomics was utilized for multivariate data analysis. In total, 27 metabolites were identified from the two species of boletes, including amino acids such as glutamate and leucine, sugars like glucose and sucrose, and alkaloids like choline. Among these, 17 metabolites were classified as differential metabolites, comprising 12 amino acids, 4 sugars, and 1 alkaloid. Differential metabolites were quantified by quantitative NMR (qNMR), and these metabolites were subsequently analyzed using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database for pathway enrichment analysis. KEGG pathway analysis revealed that the different drying methods resulted in significantly distinct metabolic pathways for these differential metabolites, resulting in the enrichment of amino acid metabolism and starch and sucrose metabolism pathways.Conclusions:This metabolomics study elucidates the differences in metabolite composition and abundance between the two species of boletes, providing a theoretical foundation for selecting appropriate drying methods for their preservation.
研究领域:
食品科学
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代谢组学
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真菌学
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产品关联
DOI
PubMed
高影响因子
PHYTOCHEMICAL ANALYSIS
·
2021
·Vol. 32
·期 6
·899-906
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IF 3.373
Songbao Zhang,Chao Li,Wei Gu,Rongli Qiu,Jianguo Chao,Lingfeng Pei,Lijie Ma,Yangfang Guo,Rong Tian
Introduction Dandelion ( Taraxacum mongolicum Hand.-Mazz.) is a perennial herb with diverse pharmacological effects. The development and utilization of dandelion have attracted much attention. Objectives Our aims were to provide a reference basis for the identification of the origin of dandelions and to study the influence of their origin on their quality. Methods High-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to analyze metabolites from dandelions from four different geographical regions in China, namely Gansu, Henan, Shanxi, and Jiangsu. Metabolite analysis was performed using orthogonal partial least-squares discriminant analysis, and to identify potential metabolic pathways, MBRole was used to perform Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Results Principal component analysis revealed that the chemical components of dandelions sampled from the four regions showed noticeable differences. Twenty-six, six, six, eight, eight, and fifteen differentially produced metabolites were identified upon comparison between Gansu and Jiangsu, Gansu and Shanxi, Gansu and Henan, Henan and Shanxi, Henan and Jiangsu, and Shanxi and Jiangsu, respectively. These differentially produced metabolites were mainly phenolic compounds. Further, KEGG pathway enrichment analysis showed that the main metabolic pathways involved were biosynthesis of phenylpropanoids and flavonoids. Conclusion The methods reported herein can be used to identify the origin of dandelions; moreover, our results can serve as a reference basis for future studies.
研究领域:
植物学
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药理学
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分析化学
✓ 已验证
DOI
高影响因子
Diversity-Basel
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2023
·Vol. 15
·期 6
·701
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IF 2.4
Meile Sun,Xiangrong Ren,Ya Liu,Jing Yang,Jingtao Hui,Yukun Zhang,Yanhua Cui,Jun Zhang,Guocang Lin,Yan Li
Roses are important plants, and they are cultivated worldwide for their beautiful flowers, remarkable scent, and nutritious hips. In this study, we re-sequenced the whole genomes of 57 rose cultivars and one wild species that originated from different regions around the world and had different scents, aiming to evaluate their genetic structure and to detect the potential signature of the selective sweep between different scent groups with single-nucleotide polymorphism (SNP) and indel markers. The roses were sequenced at an average depth of 6× to the reference genome ofRosa‘Old blush’. A total of 2,375,949 SNPs were obtained. The SNP numbers varied among the 58 samples, with an average of 1,271,906 per sample. The phylogeny and population structure revealed that the roses could be divided into three main clusters; however, the groups were not consistent in terms of geographic origin or scent classification. These indicated that rose cultivars have a complex genetic background due to the extensive hybridization between cultivated roses or wildrosaspecies worldwide. A selective sweep analysis was conducted to detect the selection signatures in rose scent traits. A total of 2430 candidate genes were identified in the strong scent groups, and were at the top 5% FSTcompared to the moderate group. These genes were significantly enriched in the KEGG pathways of tyrosine metabolism, cyanoamino acid metabolism, alpha-linolenic acid metabolism (13 genes), and phenylpropanoid biosynthesis (30 genes). When the low-scent group was used as the control, 2604 candidate genes were identified at the top 5% FSTthat were significantly enriched in the KEGG pathways of alpha-linolenic acid metabolism (15 genes), and glutathione metabolism (23 genes). We also observed genes enriched in pathways including the linoleic acid metabolism (five genes), diterpenoid biosynthesis (six genes), and monoterpenoid biosynthesis (seven genes), although they were not significant. These implied a positive selection of rose cultivars with a strong fragrance in terms of fatty acid derivatives, terpenoids and benzenoids/phenylpropanoids during rose breeding. Our study establishes a foundation for the further large-scale characterization of rose germplasm, improving the genetic knowledge of the background of roses.Keywords:single-nucleotide polymorphism (SNP);indel;genome variation;selection sweep;KEGG pathway
研究领域:
植物学
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遗传学
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分子生物学
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DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Xianbao Li,Binxu Li,Natalia S. Podio,Xiao-Yin Wang,Suhang Jiang,Shuyan Xu,Xuemei Qiu,Zheng Zeng,Wei Gong,Shirui Wang,Er Sheng Gong
Phenolics in three purple rice varieties were qualitatively and quantitatively analyzed using untargeted metabolomics, and their antioxidant activities were also investigated. Jixuenuo showed the highest antioxidant activity and phenolic content among the three varieties. Ninety-three phenolics across four categories (flavonoids, phenolic acids, stilbenes, and lignans) were identified, with Jixuenuo showing the highest anthocyanin content. Cyanidin-3 -O- glucoside (C3G) accounted for over 90% of total anthocyanin content. The volcano plot analysis identified 53 differential phenolics, 22 of which were classified as major. KEGG pathway analysis revealed significant enrichment in flavonoids biosynthesis pathways. Jixuenuo showed significantly enhanced biosynthesis in the pathways for cyanidin, dihydromyricetin, epicatechin, C3G, myricetin, and rutin. Correlation analysis indicated that these flavonoids were significantly positively correlated with antioxidant activity and total phenolic content. These findings suggest that specific flavonoids in Jixuenuo may serve as markers for evaluating the antioxidant potential of purple rice.
研究领域:
食品科学
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营养学
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代谢组学
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植物化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Lei Ye,Xuezhen Yang,Zhenzhu Huang,Bo Zhang,Qing Tian,Yu Huang,Wei Tan,Mingjun Feng,Xiaolin Li,Hongbin Xu
Driven by the need for sustainable agriculture, using Lycium barbarum (goji) sawdust as a substrate for mushroom cultivation increases productivity while reducing environmental impact. This study evaluates the effects of different proportions of goji sawdust on the growth, yield, and metabolic profiles of pink A. cornea . Six substrate formulations were tested, with 16 % goji sawdust supporting optimal yield and quality. LC-MS/MS-based metabolomics identified 1335 metabolites, mainly organic acids and derivatives, lipids and lipid-like molecules, organoheterocyclic compounds, and benzenoids. Biomarker analysis revealed twenty to thirty-five metabolites associated with substrate variations. KEGG enrichment highlighted key metabolic pathways, including ABC transporters, amino acid biosynthesis, D-amino acid metabolism, and aminoacyl-tRNA biosynthesis, contributing to substrate adaptation. While this study offers insights into the metabolic responses of pink A. cornea to goji sawdust, future research should incorporate absolute biomarkers quantification to further validate these results and optimize substrate compositions.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Frontiers
·
2023
·
IF 9.9
Guilin Chen,Yongbing Xu,Hui Zhang,Felix Wambua Muema,Mingquan Guo
Gymnema sylvestre (GS) has been traditionally used to treat diabetes mellitus (DM) and consumed as both food and medicine. The present work aimed to reveal the arresting hypoglycemic properties of GS leaves on type 2 DM (T2DM) rats meditated by gut microbiota pathway. After administration for 30 days, GS treatment observably improved the abnormal fasting blood glucose (FBG) levels, lipid profiles, and the glucose tolerance of the T2DM rats in a dose-dependent manner. Furthermore, GS treatment reversibly restored the gut microbiota diversities in the T2DM rats based on the decreased Firmicute abundance and boosted Bacteroidetes abundance and Firmicutes/Bacteroidetes ratio. Particularly, the KEGG pathway suggested that GS treatment strongly interfered with the glucose metabolism processes, such as the glycolysis from glucose 6-phosphate and glucose, along with the changes in the relative abundances of the beneficial bacteria of Clostridium , Lactobacillus , and Bacteroides . In addition, the GS treatment dramatically promoted the contents of the acetic acid, the total short-chain fatty acids in the T2DM rat feces. This study showcased the remarkable amelioration of GS on the metabolic disorders of blood glucose and lipids via the regulation of gut microbiota in T2DM rats, which could offer a new mechanism of GS as a potential natural antidiabetic food supplement for T2DM.
研究领域:
药理学
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微生物学
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内分泌学
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营养学
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产品关联
DOI
高影响因子
ENVIRONMENTAL TOXICOLOGY
·
2023
·Vol. 38
·期 4
·833-843
·
IF 4.5
Xiaofang Wang,Lingyu Hu,Cuiyuan Jin,Mingrong Qian,Yuanxiang Jin
As an effective fungicide widely used in agricultural production, the excessive procymidone (PRO) residue has been detected in the environment and food. Our previous study demonstrated that PRO could destroy the intestinal barrier in mice and has a joint toxic effect. To explore the cross-generational impact of maternal exposure, 10-week-old C57BL/6 female mice were orally administrated to 10 and 100 mg/kg body weight/day of PRO during pregnancy and lactation. The offspring obtained nutrients from the maternal through the placenta and breast milk, and PRO residues were detected in the liver, intestine, and feces of F1 generation. Fecal examination found that the residual PRO had been completely metabolized when the offspring mice grew to 35 days. The drug residue of F1 generation male mice was higher than that of female mice. We attributed this result to the difference in cytochrome P450 (CYP450) enzyme expression between male and female mice. The transcriptional levels of CYP1A1, CYP1A2, CYP2D9, and CYP3A4, and CYP450 protein expression levels, were higher in female mice. Furthermore, targeted MS of plasma revealed abnormal amino acid levels. In addition, PRO-induced hepatic metabolite changes in F0 and F1-7w mice. KEGG pathway analysis further showed that PRO jointly changed the amino acid biosynthesis pathway of the maternal and offspring. In summary, these results indicated that maternal exposure to PRO during a special period would interfere with self metabolism, and offspring will also have metabolic disorders.
研究领域:
毒理学
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药理学
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分子生物学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）