查找使用阿拉丁产品的科研文献

Creating a multifunctional degrader for co-mineralization of p-nitrophenol and 1,2-dichloroethane and its application in wastewater bioremediation

Yujie Liu,Yuting Jiang,Yan Meng,Weini Xiong,Ziling Yuan,Ruihua Liu,Chao Yang

Because the interactions among contaminants may lead to enhanced toxicity, combined pollution caused by the co-presence of multiple contaminants has increasingly gained public concern. p -Nitrophenol (PNP) and 1,2-dichloroethane (1,2-DCA) are frequently co-detected in groundwater. To completely eliminate PNP, 1,2-DCA and intermediates from polluted sites, in this study, a novel degrader KTU-PDG was created by functional assembly of PNP and 1,2-DCA biodegradation pathways in a robust chassis Pseudomonas putida KT2440. Cell growth assay indicated that PNP or 1,2-DCA can be metabolized as a sole carbon source by strain KTU-PDG for cell proliferation. Stable isotope analysis indicated that strain KTU-PDG possesses the capability of co-mineralizing PNP and 1,2-DCA to CO 2 in mineral salt medium. In wastewater bioremediation, the strain KTU-PDG was proven to be capable of co-mineralizing PNP and DCA and maintained high cell viability during bioremediation. Herein, we demonstrate for the first time co-mineralization of PNP and 1,2-DCA by a single strain. Moreover, green fluorescent protein (GFP)-labeling of strain KTU-PDG facilitates estimation of viable cell number and real-time monitoring of cellular activity and transfer by autofluorescence in the environment. These merits of strain KTU-PDG highlight great potential of this degrader for in situ bioremediation of sites co-contaminated with PNP and 1,2-DCA. More importantly, this strategy of multi-pathways assembly in an optimal chassis shows good potential for the clean-up of combined pollution caused by other organic pollutants.