DRUG RESISTANCE UPDATES
·
2026
·
IF 22
Liang Wang,Jie Zhu,Jingnan Lv,Yunmiao Xu,Jiayao Lin,Yan Qian,Qizhao Gao,Yicheng Wen,Yaxuan Zhai,Zhichen Zhu,Haifang Zhang,Liang Chen,Hong Du
Aims The worldwide spread of carbapenem-resistant Klebsiella pneumoniae (CRKP) has posted a global threat. Treatment options for CRKP are limited, especially for strains producing New Delhi metallo-β-lactamase (NDM). The emergence of the siderophore antibiotic cefiderocol has brought hope; however, recent clinical and research data show that NDM-producing K. pneumoniae have a high rate of resistance to cefiderocol, and the reason remains unclear. Methods This study focused on the dynamic changes in K.pneumoniae under host nutritional immunity and cefiderocol stress. It identified one of the main reasons for the strong cefiderocol resistance in NDM-producing strains. Further studies found that the JNMCOFLA_01041 gene plays an important role in this process and is crucial for cefiderocol resistance and virulence in K.pneumoniae . Results Under host nutritional immunity and cefiderocol stress, K.pneumoniae is exposed to increasing oxidative stress. This oxidative stress results in a dynamic pattern of rapid increases in intracellular Zn²⁺ concentrations. This autonomous changes in Zn²⁺ levels in response to cefiderocol challenge can rapidly increase NDM enzyme activity and enhance the MIC, which may be one of the main reasons for the high cefiderocol minimal inhibit concentration (MIC) in NDM-producing strains. Further study identified a key gene, JNMCOFLA_01041 , for Zn²⁺ and hemin absorption in K. pneumoniae . The Zn²⁺ acquisition function plays an important role in oxidative stress resistance, NDM activity, metabolism, and even pathogenicity. Conclusions Taken together, our research highlights the impact of dynamic changes in bacterial metal ion concentrations on bacterial survival and drug resistance under complex host conditions. It emphasizes that, while using antimicrobial drugs, monitoring and regulating changes in the bacterial internal environment under pathological conditions is crucial for maximizing anti-infective efficacy. Furthermore, JNMCOFLA_01041 could be a target of novel drug and vaccine development.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLoS Pathogens
·
2024
·Vol. 20
·期 10
·e1012675
·
IF 5.5
Lijun Wang,Zhe Wang,Hua Zhang,Qian Jin,Shuaihua Fan,Yanni Liu,Xueting Huang,Jun Guo,Chao Cai,Jing-ren Zhang,Hui Wu
Klebsiella pneumoniae , an emerging multidrug-resistant pathogen, exhibits hypermucoviscosity (HMV) as a critical virulence trait mediated by its capsular polysaccharide (CPS). Recent discoveries have determined acetylation as a significant modification for CPS, although its impact on HMV and virulence was previously unknown. This study elucidates the roles of two enzymes: K lebsiella p neumoniae A cetylated C PS E sterase (KpACE), an esterase that removes acetyl groups from CPS, and WcsU, an acetyltransferase that adds acetyl groups to CPS. KpACE is highly upregulated in an ompR -deficient mutant lacking HMV, and its overexpression consistently reduces HMV and diminishes virulence in a mouse model of pneumonia. The esterase domain-containing KpACE effectively deacetylates model sugar substrates and CPS-K2. Site-directed mutagenesis of the conserved catalytic histidine residue at position 370 significantly reduces its enzymatic activity. This reduction correlates with decreased HMV, affecting key virulence traits including biofilm formation and serum resistance. Similarly, a deficiency in the wcsU gene abolishes CPS acetylation, and reduces HMV and virulence. These results highlight the importance of the delicate balance between CPS acetylation by WcsU and deacetylation by KpACE in regulating the pathogenicity of K . pneumoniae . Understanding this balance provides new insights into the modulation of virulence traits and potential therapeutic targets for combating K . pneumoniae infections.
Klebsiella pneumoniae is an antibiotic-resistant pathogen known for its virulence factor, hypermucoviscosity (HMV), which relies on the production of capsular polysaccharides (CPS). This study highlights the roles of two key enzymes: KpACE, an esterase that removes acetyl groups from CPS, and WcsU, an acetyltransferase that adds acetyl groups to CPS. Overexpression of KpACE reduces HMV and decreases virulence in a mouse pneumonia model by deacetylating CPS. Conversely, a deficiency in the wcsU gene, which is responsible for acetylating CPS, also results in decreased HMV and virulence. The balance between CPS acetylation by WcsU and deacetylation by KpACE is crucial in regulating K . pneumoniae pathogenicity. This balance affects various virulence factors, including biofilm formation and serum resistance, highlighting the importance of CPS acetylation dynamics in the pathogen’s fitness and virulence. Understanding these mechanisms offers insights into potential therapeutic targets to mitigate the virulence and antibiotic resistance of K . pneumoniae .
✓ 已验证
产品关联
DOI
PubMed
高影响因子
iMeta
·
2026
·
IF 44.4
Tingting Xu,Xiuying Tao,Dan Fang,Haijie Zhang,Zhiqiang Wang,Yuan Liu
Carbapenem‐resistant Klebsiella pneumoniae (CRKP) is a major multidrug‐resistant pathogen with limited treatment options. Plant‐derived natural products have broad biological activities, but their protective effects against extraintestinal multidrug‐resistant bacterial infections remain poorly understood. Herein, we show that selected plant‐derived natural products protect mice against CRKP pulmonary infection by remodeling the gut microbiota. Fecal microbiota transplantation and microbiota‐depletion experiments demonstrated that this protection was microbiota dependent, with Lactobacillus emerging as a key genus associated with reduced pulmonary bacterial burden. Among four isolated Lactobacillus species, Lactobacillus murinus ( L. murinus ) conferred the strongest protection against CRKP infection. Mechanistically, L. murinus ‐derived membrane vesicles (MVs) delivered the small RNA mim‐miR‐21a‐5p to pulmonary macrophages, where it targeted PDCD4 and activated the Nrf2/SOD2 antioxidant pathway. This signaling cascade preserved mitochondrial oxidative phosphorylation, promoted an M2‐like macrophage phenotype, and ultimately restricted CRKP infection. Consistent with these findings, intestinal Lactobacillus , particularly L. murinus , was reduced in patients with CRKP infection compared with healthy controls. Collectively, our findings reveal a mechanism by which plant‐derived natural products enhance host resistance to CRKP through microbiota remodeling and identify L. murinus ‐derived small RNAs and membrane vesicles as key mediator of this gut‐lung protective axis. This study reveals that plant‐derived natural products confer protection against carbapenem‐resistant Klebsiella pneumoniae (CRKP) infection via remodeling the gut microbiota, with Lactobacillus identified as the core beneficial genus. Mechanistically, Lactobacillus murinus ( L. murinus )‐derived mim‐miR‐21a‐5p carried by membrane vesicles (MVs) targets PDCD4 . This interaction further activates the Nrf2/SOD2 signaling axis, promotes M2 macrophage polarization, and ultimately suppresses CRKP infection.
✓ 已验证
DOI
PubMed
高影响因子
Emerging Microbes & Infections
·
2026
·
IF 7
Yaxuan Zhai,Qiyuan Jin,Jiawen Shi,Xiaoyun Zhang,Yan Qian,Yicheng Wen,Jie Zhu,Zhichen Zhu,Liang Wang,Qizhao Gao,Tao Wang,Wanxiang Li,Haifang Zhang,Liang Chen,Hong Du
The global emergence of hypervirulent Klebsiella pneumoniae (hvKP) and extensively drug-resistant (XDR) phenotypes has precipitated a severe public health crisis with limited therapeutic options. In particular, the hypervirulent ST65 and ST23 lineages, alongside the carbapenem-resistant ST11 lineage, have been reported to be emerging globally with the potential for continued large-scale dissemination. Despite the urgent need for preventive strategies, the regulatory networks governing hvKP virulence and their potential as targets for live attenuated vaccines remain inadequately explored. In this study, we elucidate a pathogenic mechanism driven by the transcription factor RpoE. Deletion of the rpoE gene in hvKP significantly attenuated virulence by directly downregulating the expression of type 3 fimbriae (T3F) and subsequently impairing biofilm formation. Based on this marked virulence attenuation, we evaluated the ΔrpoE strain as a potential live attenuated vaccine candidate. Remarkably, immunization elicited protective immune responses involving functional antibody responses and CD4⁺ T cell-associated immunity, conferring host protection against the tested ST11, ST23, and ST65 clinical isolates. Our findings define an RpoE-T3F regulatory axis involved in hvKP virulence and support ΔrpoE as a potential live attenuated vaccine candidate with protective efficacy against these tested clinically relevant KP strains.
✓ 已验证
DOI
高影响因子
iMeta
·
2026
·
IF 44.4
Hongquan Chen,Xiaoling Wang,Xiang Liu,Kui Dong,Yanying Feng,Min Yuan,Shiyi Wang,Fangfang Fan,Yong Tian,Zhiming Kang,Xiushuai Du,Chaolu Hasi,Hong Lu,Wei Guan,Xu Chen,Mengyu Cheng,Wei Wei,Nan Guo,Zhiguo Liu,Xiaotong Qiu,Yiwei Shi,Kun Xiao,Yi Wang,Zhenjun Li
Severe Klebsiella pneumoniae pneumonia is associated with substantial mortality and is characterized by intense systemic inflammation that coexists with a failure to control infection. The cellular changes underlying the transition from effective host defense to immune dysfunction remain incompletely defined. Here, we constructed a severity-focused single-cell transcriptomic atlas of 708,894 peripheral blood mononuclear cells from 100 individuals (54 healthy controls, 23 patients with mild disease, and 23 patients with severe disease), complemented by plasma protein measurements and flow cytometry. Mild disease was characterized by a relatively coordinated peripheral immune response, with preserved monocyte regulatory and tissue-repair programs, preserved cytotoxic lymphocyte activity, and coordinated interactions between T follicular helper cells and B cells. Severe disease showed marked monocyte inflammation together with widespread disruption of adaptive immune responses. Classical monocytes expanded and showed increased expression of S100A8/A9/A12 , accompanied by enhanced TLR4–MYD88 signaling and elevated plasma S100 proteins. These changes were accompanied by impaired dendritic cell antigen presentation, expansion of immunoregulatory monocyte states resembling monocytic myeloid-derived suppressor cells, cytotoxicity- and exhaustion-associated changes in CD8 + T cells and natural killer cells, hyperactivated yet poorly coordinated CD4 + T cell states, and weaker B cell receptor signaling despite plasma cell expansion. These findings delineate a severity-associated peripheral immune state characterized by monocyte-centered inflammatory signatures and features of altered adaptive immune coordination, and provide a basis for future mechanistic investigation of monocyte-derived S100 alarmins and severity-associated myeloid remodeling. Graphical Severe Klebsiella pneumoniae pneumonia combines intense inflammation with failure to control infection, yet the cellular basis is unclear. Using a severity-focused single-cell atlas of 708,894 peripheral blood mononuclear cells from 100 individuals, complemented by plasma protein measurements and flow cytometry, we show that disease severity redirects rather than simply amplifies systemic immunity. Mild disease preserves coordinated host defense: immune-regulatory and tissue-reparative monocytes, intact cytotoxic lymphocytes, and T follicular helper–B cell coordination. Severe disease is characterized by S100A8/A9/A12 -high inflammatory monocytes with enhanced TLR4–MYD88 signaling, expansion of monocytic myeloid-derived suppressor cell-like states, and impaired dendritic cell antigen presentation. These myeloid changes accompany dysfunctional CD4 + T cells, cytotoxicity- and exhaustion-associated CD8/NK cells, and weakened expression of B cell receptor signaling-related genes despite plasma cell accumulation. Severe disease is characterized by a systemic pattern of impaired immune coordination, highlighting monocyte-derived S100 alarmins as candidates for future mechanistic and therapeutic investigation. Highlights Klebsiella pneumoniae pneumonia severity maps to distinct peripheral immune states, with relatively coordinated responses in mild disease and altered immune coordination in severe disease. S100A8/A9/A12 -high classical monocytes characterize the inflammatory landscape of severe disease. Severe disease features immunoregulatory monocyte expansion and reduced dendritic cell antigen presentation programs. CD8/NK and CD4 + T cell remodeling accompanies altered adaptive immune coordination in severe disease.
✓ 已验证
DOI
高影响因子
Frontiers in Microbiology
·
2023
·
IF 5.2
Wei Liu,Guang Chen,Keke Dou,Bingcheng Yi,Danyang Wang,Qihui Zhou,Yunbo Sun
Multidrug-resistant (MDR) bacterial infections have gained increasing attention due to the high incidence rates and high mortality, especially for the carbapenem-resistant Klebsiella pneumoniae (CRKP) infection that can cause severe complications (e.g., pneumonia and sepsis) in multiple organs. Therefore, the development of new antibacterial agents against CRKP is imperative. Inspired by natural plant antibacterial agents with broad-spectrum antibacterial properties, the antibacterial/biofilm activity of eugenol (EG) on CRKP and their underlying mechanisms are investigated in our work. It is found that EG exhibits remarkable inhibitory effects on planktonic CRKP in a dose-dependent fashion. Meanwhile, the destruction of membrane integrity induced by the formation of reactive oxygen species (ROS) and glutathione reduction results in the leakage of bacterial cytoplasmic components, including DNA, β-galactosidase, and protein. Moreover, when EG contacts with bacterial biofilm, the whole thickness of the dense biofilm matrix decreases, and the integrity is destroyed. Overall, this work verified that EG could eliminate CRKP via ROS-induced membrane rupture, which offers vital evidence to explain the antibacterial ability of EG against CRKP.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2025
·
IF 4.7
Yongqing Liu,Tingting Yan,Hongtao Kong,Leizi Chi,Dejun Liu,Shangshang Qin,Yang Wang,Muchen Zhang,En Zhang
As antibiotic resistance increases and new drug development lags, the effectiveness of single antibiotics has drastically diminished for clinical infection treatment. The resistance crisis is exacerbated by the swift development and dissemination of Gram-negative bacteria that are extensively drug-resistant (XDR), particularly strains resistant to carbapenems in clinical settings. In this scenario, antibiotic adjuvants play a crucial role in revitalizing existing treatments to combat carbapenem-resistant bacterial infections. In this study, we synthesized ten small molecular antimicrobial peptide mimics and identified antimicrobial peptide mimic 329 (A329), which exhibited a synergistic effect with minocycline (FICI = 0.023), enhancing its efficacy by 4- to 128-fold, and significantly enhanced the antibacterial efficacy of minocycline against carbapenem-resistant Klebsiella pneumoniae (CRKP, 0.015 ≤ FICI ≤0.141), prevented the emergence of minocycline resistance, improved the survival rate of mice, and decreased bacterial load in tissues at 8 + 8 mg/kg. Mechanistic studies revealed that A329 increases bacterial membrane permeability and disrupts the proton-motive force. Additionally, A329 combined with minocycline boosts intracellular minocycline accumulation, induces intracellular production of reactive oxygen species (ROS), and ultimately triggers bacterial death. These findings advised that A329 in combination with minocycline is a potential combination therapy against XDR-associated infections.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLoS Pathogens
·
2025
·Vol. 21
·期 1
·e1012787
·
IF 4.9
Tingting Wang,Huaizhi Yang,Qiushuang Sheng,Ying Ding,Jian Zhang,Feng Chen,Jianfeng Wang,Lei Song,Xuming Deng
Hypervirulent Klebsiella pneumoniae (hvKP) poses an alarming threat in clinical settings and global public health owing to its high pathogenicity, epidemic success and rapid development of drug resistance, especially the emergence of carbapenem-resistant lineages (CR-hvKP). With the decline of the “last resort” antibiotic class and the decreasing efficacy of first-line antibiotics, innovative alternative therapeutics are urgently needed. Capsule, an essential virulence determinant, is a major cause of the enhanced pathogenicity of hvKP and represents an attractive drug target to prevent the devastating clinical outcomes caused by hvKP infection. Here, we identified isoferulic acid (IFA), a natural phenolic acid compound widely present in traditional herbal medicines, as a potent broad-spectrum K . pneumoniae capsule inhibitor that suppresses capsule polysaccharide synthesis by increasing the energy status of bacteria. In this way, IFA remarkably reduced capsule thickness and impaired hypercapsule-associated hypermucoviscosity phenotype (HMV), thereby significantly sensitizing hvKP to complement-mediated bacterial killing and accelerating host cell adhesion and phagocytosis. Consequently, IFA facilitated effective bacterial clearance and thus remarkably protected mice from lethal hvKP infection, as evidenced by limited bacterial dissemination and a significant improvement in survival rate. In conclusion, this work promotes the development of a capsule-targeted alternative therapeutic strategy for the use of the promising candidate IFA as an intervention to curb hvKP infection, particularly drug-resistant cases.
Hypervirulent Klebsiella pneumoniae (hvKP) is becoming an imminent threat to public health because of the constant emergence of drug resistance and its devastating clinical outcomes. Meanwhile, the both notorious phenotypes are integrated to create carbapenem-resistant and hypervirulent K . pneumoniae (CR-hvKP), which leads to a scarcity of effective treatments. Therefore, there is an increasing urgent need for new therapeutics other than antibiotics. The indispensable role of capsule in immune evasion and pathogenesis renders it a promising target for developing novel alternative strategies to combat hvKP infections. In this study, we identified the small natural compound isoferulic acid (IFA) as a potent broad-spectrum inhibitor of K . pneumoniae capsule. Hypercapsule-associated hypermucoviscosity (HMV), a characteristic phenotype that has been shown to play a distinct and critical role in the pathogenesis of hvKP, is significantly impaired by IFA. RNA-seq analysis revealed that IFA interferes with the biosynthesis of capsule polysaccharide through altering bacterial metabolism status. Consequently, IFA sensitizes hvKP to serum killing and impedes capsule- and HMV-mediated resistance to adherence and phagocytosis. In vivo, IFA treatment facilitates effective host clearance and thereby systemically protects mice against hvKP infection. Our research sheds light on the development of capsule-targeted drug and provides an effective lead compound that may contribute to the management of hvKP infections as an intervention.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2026
·
IF 5.8
Menglu Mao,Keke Li,Chenyao Lin,Xuedan Qiu,Zhen Zhang,Dumei Ma,Qingcao Li
IntroductionEarly and precise identification of Klebsiella pneumoniae carbapenemase (KPC) is critical for guiding effective treatment and curbing the spread of carbapenem-resistant pathogens. The mainstream detection methods targeting KPC are exclusively qualitative, lacking reliable quantitative approaches. To address the need for a rapid and quantitative method, this study developed a sensitive fluorescence immunoassay.MethodsThe assay employs a sandwich immunoassay format, which utilizes carboxylated magnetic agarose microspheres for capture and time-resolved fluorescent microspheres for detection. It enables quantification of the KPC protein within approximately 1 h. The analytical performance of the assay was systematically evaluated, including its sensitivity, linear range, specificity and accuracy.ResultsUnder optimal experimental conditions, it exhibits a wide linear range (0.25–1000 ng/mL) and a low detection limit of 0.194 ng/mL, and shows no cross-reactivity with other common carbapenemases. Recovery rates in bacterial lysates ranged from 96.7 to 108.4%. Compared to a commercial immunochromatographic test, this method demonstrated superior sensitivity.DiscussionThe primary advantage of this fluorescent immunoassay is its ability to achieve rapid, sensitive and quantitative detection of KPC, which addresses a key limitation of current qualitative methods. Its wide linear range and low detection limit enable precise monitoring of KPC expression level variations, offering a novel tool for the clinical assessment of bacterial resistance burden.
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2022
·
IF 6.064
Liu Jun,Ding Hui,Zhao Mingjie,Tu Fan,He Tian,Zhang Lizhu,Jing Yanfei,Rui Xiaohong,Zhang Shiliang
Sepsis is a systemic inflammatory response syndrome caused by infection, with high incidence and mortality. Therefore, it is necessary to carry out an effective anti-infection treatment. In this work, we designed and synthesized red blood cell (RBC) membrane-coated PLGA nanoparticles named γ3-RBCNPs, which target the highly expressed intercellular adhesion molecule-1 (ICAM-1) at the site of infection through the γ3 peptide on its surface and kill the Klebsiella pneumoniae through ciprofloxacin encapsulated in its core. In addition, the homogenous RBC membrane coated on the surface of the nanoparticles helps them avoid immune surveillance and prolong the circulation time of the drug in the body. We found that the γ3-RBCNPs target human umbilical vein endothelial cells (HUVECs) activated by TNF-α in vitro and the infected lung of mice in the sepsis model very well. In vitro evaluation suggested that γ3-RBCNPs have a low risk of acute hemolysis and are less likely to be engulfed by macrophages. In vivo evaluation showed that γ3-RBCNPs has a long half-life and good bio-safety. More importantly, we confirmed that γ3-RBCNPs have the good antibacterial and anti-infection ability in vivo and in vitro. Our research provides a new strategy for the nano-drug treatment of Klebsiella pneumoniae-induced sepsis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Wenjie Yu,Yifan Zhang,Jinwang Li,Junru Li,Keju Li,Fangjian Ning,Xiali Guo,Xueyong Huang,Liping Luo
Scrophularia ningpoensis honey (SNH) is a type of honey derived from a traditional Chinese medicinal plant, with considerable potential value. Klebsiella pneumoniae , one of the most threatening pathogens worldwide, is also among the leading causative agents of wound infections, postoperative complications, and trauma-related skin and soft tissue infections. However, research on SNH remains limited, particularly regarding its compound composition and its ability to combat K. pneumoniae . In this study, the physicochemical properties of SNH were comprehensively characterised, revealing its high quality. SNH also demonstrated superior antibacterial activity compared to seven other types of honey. Furthermore, pathways such as valine, leucine, and isoleucine biosynthesis, as well as pantothenate and CoA biosynthesis, have been found to contribute to the antibacterial activity of SNH. To identify the active constituents, SNH was sequentially extracted with solvents of varying polarities and analyzed by UPLC-MS/MS, resulting in the identification of 157 metabolites. Among them, chlorogenic acid and chrysin were identified as potential key compounds responsible for the antibacterial effects against K. pneumoniae . This study systematically elucidated, for the first time, the physicochemical characteristics and chemical composition of SNH and demonstrated its strong antibacterial properties, providing an experimental basis for its further development and application.
✓ 已验证
产品关联
DOI
高影响因子
Infection and Drug Resistance
·
2025
·
IF 2.9
Xuan Teng,Kexue Yu,Qi Zhang,Chengcheng Ma,Wenwen Chu,Chengyin Shen,Zhou Liu
Purpose To systematically analyse and identify volatile organic compounds (VOCs) released by clinical Klebsiella pneumoniae (K. pneumoniae) during growth via proton transfer reaction–mass spectrometry (PTR-MS), aiming to establish a rapid and accurate method for differentiating and identifying carbapenem-resistant Klebsiella pneumoniae (CRKP) and KPC and NDM producers.Methods Nonrepetitive clinical strains isolated from patient specimens were collected from September 2021 to May 2025. The strains were subjected to drug susceptibility testing and carbapenemase genotype identification via the VITEK2 system and polymerase chain reaction (PCR). The clinical strains were incubated in a closed system under the combined pressure of meropenem (MEM) and carbapenemase inhibitors for 3 h. PTR-MS was used to monitor the inhibition rate of the characteristic VOC ion signal intensity to obtain drug susceptibility information of KP and carbapenemase type. Characteristic ions were characterized via fast gas chromatography (FGC)-PTR-MS.Results A total of 105 clinical isolates, including 53 carbapenem-susceptible Klebsiella pneumoniae (CSKP) isolates and 52 CRKP (43 KPC-positive and 9 NDM-positive) isolates, were collected. With MEM, the sensitivity and specificity of PTR-MS for monitoring CRKP were 98.08% and 100.00%, respectively. In the case of MEM combined with different carbapenemase inhibitors, the assay was evaluated using a subset of isolates (n=31), comprising 22 KPC-positive and 9 NDM-positive strains. The sensitivity and specificity of PTR-MS for monitoring single KPC producers were 90.91% and 100.00%, respectively, and those for single NDM producers were 88.89% and 100.00%, respectively (kappa= 0.853 and 0.919 for KPC- and NDM-positive strains, respectively). FGC–PTR-MS analysis indicated that the VOCs corresponding to these characteristic ions were acetaldehyde, ethanol and acetic acid.Conclusion Real-time monitoring by PTR-MS of the dynamic release characteristics of specific VOC ions in the headspace of CRKP within 3 h under the combined stress of antibiotics and carbapenemase inhibitors can provide important information for rapidly identifying CRKP and the main clinical carbapenemase types.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）