JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Juan Han,Hui Feng,Jiacong Wu,Yuanyuan Li,Yang Zhou,Lei Wang,Peng Luo,Yun Wang
Today, we are seeking an efficient biotransformation of cellulosic material into sustainable biochemical products to meet the increasing global energy demand. Herein, we report the fabrication of multienzyme hybrid nanoflowers (ECG-NFs) by co-immobilizing three recombinant enzymes (cellobiohydrolase (CBH), endo-glucanase (EG), and β-glucosidase (BG)) integrating a binary tag composed of elastin-like polypeptide (ELP) and His-tag to act as a tri-enzyme biocatalyst, which catalyzes the hydrolysis of cellulose into glucose. The prepared ECG-NFs exhibited excellent performance in terms of pH stability, thermal stability, storage stability, and catalytic efficiency compared to free multienzyme system. Notably, ECG-NFs could be recycled for up to eight consecutive runs. The Km and kcat/Km values for ECG-NFs were 9.33 g L–1 and 0.0051 L min–1 g–1, respectively, which were better than those of the free multienzyme system, indicating a better substrate affinity. Finally, the overall enzyme activity of ECG-NFs increased by 1.12 times and the degradation efficiency of ECG-NFs was superior to the free multienzyme system, which revealed that ECG-NFs could facilitate an effective one-pot hydrolysis of cellulose into glucose.
研究领域:
生物化学
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生物技术
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材料科学
·
酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Tianyu Wang,Jianxing Feng,Hao Sun,Yanmin Liang,Ting Du,Jie Dan,Jianlong Wang,Wentao Zhang
Metal aerogel with porous structure and interlocking pore channel can provide abundant active sites to boost the peroxidase (POD)-like activity for high-performance colorimetric analysis of food quality. Herein, CuBi bimetallic aerogels with different atomic ratio (Cu 1 Bi 9 , Cu 5 Bi 5 and Cu 9 Bi 1 ) were fabricated by a facile one-step reduction strategy, and the POD-like activity of the synthesized CuBi bimetallic aerogels was studied. The specific activity of Cu 9 Bi 1 aerogel was significantly enhanced due to the Bi-doping strategy. Through comparing the K m values we find that CuBi bimetallic aerogel can be used as a cheap alternative to equivalent noble metal materials for biosensor applications. Then, the optimized nanozyme was exploited to establish a colorimetric method for the total antioxidant capacity (TAC) by utilizing ascorbic acid (AA) as a representative antioxidant model. The linear range and limit of detection (LOD) were determined to be 300–900 μM and 59.4 μM, respectively, the wide detection range simplifies sample pre-treatment steps. Additionally, the method also demonstrated good feasibility for the TAC of food samples. The current study expands new fields for the application of metal aerogels and successfully developed macroscopic nanozymes with a convenient application, facile preparation and low cost.
研究领域:
纳米技术
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分析化学
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食品科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Wenfei Dong,Guo Chen,Lin Zhang,Haiyan Cao,Wenbing Shi,Hongmei Lan,Hao Zhou
The active site of natural horseradish peroxidase (HRP) comprises iron coordinated equatorially by a porphyrin ring and axially by the imidazole group of His170. The low water solubility of porphyrin rings inspired us to investigate the potential of the iron-imidazole group as peroxidase mimic. Herein, iron-imidazole (Fe-MIM) was one-pot constructed using ascorbic acid stabilized Fe 2+ and 2-methylimidazole in the presence of zinc acetate at room temperature in aqueous medium, along with the formation of ZIF-8 framework around Fe-MIM sites. Fe-MIM/ZIF-8 was characterized by scanning electron microscopy, transmission electron microscopy, X-ray diffractometry, X-ray photoelectron spectroscopy, and Brunauer–Emmett–Teller surface measurements. Fe-MIM/ZIF-8 exhibits high peroxidase-like activity, efficiently catalyzing the redox reaction between H 2 O 2 and 3, 3′, 5, 5′-tetramethylbenzidine, as made evident by the appearance of a UV–Vis signal at 652 nm. Fe-MIM/ZIF-8 displays higher catalytic activity than the material (Fe + MIM) prepared by ascorbic acid stabilized Fe 2+ and MIM, due to its rich biomimetic architecture and large BET surface area. The K m of Fe-MIM/ZIF-8 for H 2 O 2 was 28 μM, which is 132 and 98 times lower than those of HRP and hemin (iron III porphyrin), respectively. This low K m value indicates the strong affinity of Fe-MIM/ZIF-8 for H 2 O 2 , which proves the superiority of biomimetic Fe-MIM sites and suggests Fe-MIM/ZIF-8′s potential for H 2 O 2 -related biosensing. The highly sensitive colorimetric methods for H 2 O 2 and H 2 O 2 -related glucose detection has been proposed. The applicability of this method to glucose detection in serum samples was tested with satisfactory results.
研究领域:
材料科学
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分析化学
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生物化学
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Xue Wang,Futing Wen,Liu He,Jiangyu Su,Ping Jiang,Daiping He
Defects engineering as an effective strategy was widely utilized to modulate catalytic performance for heterogeneous catalysts through tuning surface electronic surface and creating more active sites. In this work, porous Co–Mn oxide nanosheets with abundant oxygen vacancies (OVs-CMO NSs) were prepared via a simple “one-stone-two-birds” method. OVs were verified by various characterizations and found more favorable for O 2 absorption and activation to produce more reactive O2•- radical, resulting in an improved oxidase-like activity in initiating 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation , with a lower K m value of 0.00717 mM and a higher V max of 2.086 × 10 −7 M/s. Since heparin (Hep) shows an inhibition effect on OVs-CMO NSs-TMB chromogenic system due to strong absorption, a colorimetric sensing platform for Hep detection was established. The present colorimetric detection shows high sensitivity, good selectivity and reliability for normal goat serum. This work not only provides a high-performance non-precious metal oxidase mimic prepared by simple surface defect engineering, but also builds a sensitive colorimetric method for Hep detection.
研究领域:
材料科学
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纳米技术
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分析化学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 30
·6459-6468
·
IF 5.344
Xiaodan Zhang,Yuwan Lu,Qiumeng Chen,Yuming Huang
A self-templated strategy was adopted to design hollow Co3O4/MO3 (M = Mo, W) mixed-metal oxides via the Mo or W doping of ZIF-67, and subsequent pyrolysis under an atmosphere of air at a low temperature of 450 °C. The hollow Co3O4/MO3 (M = Mo, W) mixed-metal oxides displayed tunable oxidase-like and peroxidase-like activities able to efficiently catalyze the oxidation of TMB to generate a deep blue color in the absence or presence of H2O2. Relative to that of the un-doped Co3O4, the oxidase mimic activity of the Mo-doped Co3O4 increased to 1.3 to 2.1-fold, while its peroxidase mimic activity increased to 7.1 to 19.9-fold, depending on different Mo doping amounts. The oxidase mimic activity of the W-doped Co3O4 increased to 2.1 to 2.3-fold, while its peroxidase mimic activity increased to 4.8 to 5.9-fold, depending on the different W doping amounts. The Mo- and W-doped Co3O4 nanohybrid exhibited both higher O2 and H2O2 activating capability, and their H2O2 activating capacity was superior to the O2 activating capability. Furthermore, the Mo- and W-doped Co3O4 nanohybrids exhibited similar O2 activating abilities, while the Mo-doped one displayed a higher H2O2 activating capability than the W-doped one. The discrepant peroxidase-like nature of Mo- and W-doped Co3O4 nanohybrids is likely attributed to their different catalytic mechanisms. The peroxidase-like activity of Mo-doped Co3O4 is highly related to the ˙OH free radical, while that of W-doped Co3O4 is likely ascribed to the electron transfer between TMB and H2O2. The Km values of Co3O4/MoO3 for TMB and H2O2 were 0.0352 mM and 0.134 mM, which were 3.2- and 1.9-fold lower than that of pure Co3O4, respectively. A Co3O4/MoO3-based colorimetric platform was developed for the determination of H2O2 in the 0.1–200 μM range, with a limit of detection of 0.08 μM (3σ). Based on the thiocholine (TCh) inhibition of the excellent peroxidase-like activity of Co3O4/MoO3 and the TCh generation via acetylcholinesterase (AChE) catalyzed hydrolysis of acetylthiocholine chloride (ATCh), the colorimetric platform was extended to screen AChE activity and its inhibitor.
研究领域:
纳米材料
·
酶模拟
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分析化学
·
生物传感器
·
催化
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Yaoyu Tan,Mengyao Qi,Huan Jiang,Baihui Wang,Xia Zhang
The level of uric acid is crucial to human health. Octahedral oxygen vacancy MnCo 2 O 4 /Ag (V O -MnCo 2 O 4 /Ag) nanozyme was successfully prepared by simple hydrothermal, calcination and self-reduction methods. V O -MnCo 2 O 4 /Ag nanozyme is rich in Mn 2+ /Mn 3+ and C O 2+ /C O 3+ redox electron pairs, large specific surface area and oxygen vacancies. V O -MnCo 2 O 4 /Ag nanozyme showed high uricase-like activity and peroxidase-like activity. At the same time, the SERS signal of the detected molecule could be significantly enhanced after the catalytic reaction of the V O -MnCo 2 O 4 /Ag nanozyme. The K m values of V O -MnCo 2 O 4 /Ag nanozyme for H 2 O 2 and TMB were 0.04 mM and 0.027 mM respectively. Based on the uric acid oxidase-like and peroxidase-like activities of V O -MnCo 2 O 4 /Ag, we developed a label-free, sensitive, and reliable SERS uric acid detection system. The detection linear range of uric acid is 0.01 μM–1000 μM and the detection of limit is 7.8 × 10 −9 M. The results show that the sensing system has good accuracy, sensitivity, selectivity , and stability. It can be applied to the determination of samples under different conditions. This study provides profound insights into the design of enzyme-like activity regulation and SERS properties regulation of nanozymes, provides guidance for the study of reaction kinetics and catalytic mechanism of nanozymes, and has broad application prospects in the field of nanozymes and SERS sensing analysis.
研究领域:
纳米技术
·
分析化学
·
生物化学
·
材料科学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）