Lin Zhou,Faqin Dong,Wei Zhang,Yuheng Chen,Lei Zhou,Fei Zheng,Zhenzhen Lv,Jingyuan Xue,Dengliang He
The biosorption and biomineralization behavior of U(VI) by Kocuria rosea with uranium resistance higher than other general microorganisms was investigated in this study. The results showed the obvious effects of initial U(VI) concentration, biomass, time, and especially pH, and presented that U(VI) was immobilized to K. rosea by physical and chemical action. The characterization results for the precipitation proved that U–P minerals with U(VI) (H 3 OUO 2 PO 4 ·3H 2 O, H 2 (UO 2 ) 2 (PO 4 ) 2 ·8H 2 O) or U(IV) (CaU(PO 4 ) 2 ) were dominant, and the crystallization level increased with time. In the process, the phosphorous containing groups, amino, hydroxyl and carboxyl groups played important roles in adsorption of U(VI), and the phosphate groups were crucial in immobilization of uranium, showing the importance of groups containing phosphorus in both biosorption and biomineralization processes. Our findings focus on the biosorption and biomineralization mechanism of U(VI) by K. rosea , emphasize the synergy of physical adsorption and chemical immobilization in the process and formation of U(VI)–P and U(IV)–P minerals, and highlight the significance of phosphorus involvement in the reaction.
Lin Zhou,Wei Zhang,Xiangyu Xi,Jiao Zhang,Mengyu Zhou,Gaofeng Wang,Jianxi Zhu,Faqin Dong
Uranium-contaminated sites are frequently characterized by high-intensity radiation, which poses a substantial challenge to traditional bioremediation technologies. While microbial interactions with uranium are extensively studied, their efficiency and mechanisms under radiation remain poorly understood. Here, we present a radiation-tolerant microbe, Kocuria rosea , and its removal mechanism for U(VI) in an irradiated system. We reveal remarkable tolerance of Kocuria rosea to radiation and uranium, showing 40.9% of cells survive after 6000 Gy irradiation (25 Gy/min) in 50 mg/L uranium stress. We demonstrate a radiation-dependent shift in the removal mechanism of U(VI)—from biosorption and biomineralization forming H 2 (UO 2 ) 2 (PO 4 ) 2 ⋅8 H 2 O and CaU(PO 4 ) 2 in non-irradiated systems to enhanced reduction and inhibited biomineralization under irradiation—responsible for 42% and 7.23% improvement in U(VI) immobilization and reduction capacities, reaching 238.12 mg/g removal capacity. This work advances our understanding of the removal mechanism of microorganisms for uranium under irradiation, offering a new, efficient microbial remediation strategy for radionuclides.
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