CHROMATOGRAPHIA
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2023
·Vol. 86
·期 7
·541-551
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IF 1.7
Guo Xinyuan,Shang Panpan,Wei Benben,Du Wenrong,Lan Yong,Bai Ligai,Qiao Xiaoqiang,Ma Zhengyue
This study presents the development of three new chiral stationary phases. Three new peptide chiral stationary phases with a main chain of l- proline, l- phenylalanine, l- valine and l- leucine and different terminals of l- citrulline, l- lysine and l- tryptophan immobilized on 3-aminopropyltrimethoxysilane modified silica gel were prepared; furthermore, successful analyses and characterizations were conducted using Fourier transform infrared spectra, elemental analysis, and thermogravimetric analysis. After this, the enantioselective performance of the three peptide stationary phases columns was evaluated. The evaluation used nine racemic compounds under normal-phase high-performance liquid chromatography mode. Optimized enantiomeric separation conditions were established. Under these conditions, the resolutions of flurbiprofen, naproxen, benzoin, 1 ,1ʹ-bi-2-naphthol and ketoprofen on the CSP-1 column were 1.61, 2.0, 0.62, 0.52 and 1.20, respectively. In addition, the reproducibility of the CSP-1 column was also investigated. The results of the investigation illustrated that the stationary phases have good reproducibility (RSD = 0.12%, n = 5).
研究领域:
分析化学
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色谱学
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材料科学
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分离科学
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DOI
高影响因子
JOURNAL OF FLUORESCENCE
·
2025
·
IF 3.1
Chen Xin,Wang Qing-wan,Zhan Tong-ling,Sun Yue,Zhang Xiao-rui,Liu Kun,Liu Xiao-wu
Nitrogen@Carbon quantum dots (N@CQDs) are prepared using microwave hydrothermal method, and polyvinylpyrrolidone (PVP) and melamine are used as mixed C source and N source. Microwave reaction conditions of preparing the N@CQDs are 170 ℃ and 3 h. This N@CQDs are are used as fluorescence probe for detection of amino acids. The N@CQDs can recognize L-alanine in water solution, and simultaneously recognize both L-methionine and L-cysteine in dimethyl sulfoxide (DMSO) solution. The linear ranges for L-alanine, L-methionine and L-cysteine are 0–15 µM, 2–50 µM and 0–17 µM, respectively. While limits of detection (LOD) for them are 11.092 nM, 7.8067 nM and 0.67476 nM, respectively. Compared to presently available detection methods, as-prepared N@CQDs have the advantage of high sensitivity and selectivity. Furthermore, coexistence of other amino acids has minimal interference in the detection of L-alanine, L-methionine and L-cysteine. For the as-prepared N@CQDs, solvent plays important roles in influencing the identification of amino acid types and detection sensitivity.
研究领域:
分析化学
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生物传感
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材料科学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2013
·
IF 3.498
Wei Bian,Jing Ma,Wenrong Guo,Dongtao Lu,Meng Fan,Yanli Wei,Yingfu Li,Shaomin Shuang,Martin M.F. Choi
N -Acetyl-L-cysteine (NAC) and L-cysteine (Cys) capped Mn doped ZnS quantum dots (NAC-Mn/ZnS QDs and Cys-Mn/ZnS QDs) are firstly prepared by hydrothermal methods . These QDs display strong phosphorescence emission peaks at 583 and 580 nm upon excitation at 315 and 306 nm, respectively. Since their room-temperature phosphorescence is efficiently quenched by L-ascorbic acid (AA), they have been employed as phosphorescence probes for detecting AA. The linear working ranges are 2.5–37.5 and 2.5–47.5 µM and the limits of detection are 0.72 and 1.38 µM for NAC-Mn/ZnS QDs and Cys-Mn/ZnS QDs, respectively. The possible quenching mechanisms have been discussed in detail. The QDs probes are highly selective to AA over other common ions, amino acids , glucose and bovine serum album. Finally, they have been applied successfully for detection of AA in human urine samples with satisfactory results. The recoveries are 98–104%. Our work provides a simple and convenient phosphorescence method to determine AA in real samples.
研究领域:
分析化学
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生物医学
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产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2020
·Vol. 192
·期 3
·935-951
·
IF 2.277
Guo Zongren,Long Liangkun,Ding Shaojun
L-Ribulose and L-ribose are two high-value unnatural sugars that can be biosynthesized by sugar isomerases. In this paper, an L-arabinose isomerase (BvAI) from Bacillus velezensis CICC 24777 was cloned and overexpressed in Escherichia coli BL21 (DE3) strain. The maximum activity of recombinant BvAI was observed at 45 °C and pH 8.0, in the presence of 1.0 mM Mn 2+ . Approximately 207.2 g/L L-ribulose was obtained from 300 g/L L-arabinose in 1.5 h by E. coli harboring BvAI. In addition, approximately 74.25 g/L L-ribose was produced from 300 g/L L-arabinose in 7 h by E. coli co-expressing BvAI and L-RI from Actinotalea fermentans ATCC 43279 (AfRI). This study provides a feasible approach for producing L-ribose from L-arabinose using a co-expression system harboring L-Al and L-RI.
研究领域:
生物技术
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生物化学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Crystals
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2020
·Vol. 10
·期 9
·781
·
IF 2.404
Qintong Huang,Rui Li,Guangying Fu,Jiuxing Jiang
ZSM-5 zeolites are commonly used as a heterogeneous catalyst for reactions. Four ZSM-5 catalysts (with various crystallite sizes and a similar ratio of Si/Al) and their ball-milling/surface-poisoning derivates were used to convert L-lactic acid to L, L-lactide. The reaction products were analyzed by three independent analytical methods (i.e., Proton nuclear magnetic resonance (1H NMR), high-pressure liquid chromatography (HPLC), and chiral gas chromatography (GC)) for determining the L, L-lactide yield and L-lactic acid conversion. A clear size effect, i.e., smaller catalysts providing better performance, was observed. Further ball-milling/surface-poisoning experiments suggested that the size effect of the ZSM-5 catalysts originated from the diffusion-controlled nature of the reaction under the investigated conditions.Keywords:ZSM-5 zeolites;L,L-lactide;size effect;diffusion control
研究领域:
催化化学
·
材料科学
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DOI
高影响因子
Soft Matter
·
2025
·
IF 2.8
Huimin Han,Qingjing Niu,Kongying Zhu,Xiaoyan Yuan,Lixia Ren
Amphiphilic copolymers are reported to show excellent ice control activity for their special interaction with ice and water. In this work, amphiphilic L-methionine sulfoxide and L-alanine copolymers (MSACPs) with different block ratios (PMeto48-b-PAlay), different molecular weights and random (P(Meto48-co-Ala20)) structures are prepared, respectively, to study the structure and ice control activity relationship. The ice control activity is well studied using ice nucleation, dynamic ice shaping (DIS) and ice recrystallization inhibition (IRI). The MSACPs are self-assembled into micelles of 11 to 80 nm in aqueous solution, which promotes the heterogeneous ice nucleation. Among block copolymers with different block ratios (PMeto48-b-PAlay) and molecular weights, PMeto48-b-PAla20 with an appropriate block ratio and molecular weight shows the highest nucleation temperature (−18.2 °C), and excellent DIS and best IRI (the mean largest grain size is 34%) activities. And, for the block copolymers with a similar block ratio to that of PMeto48-b-PAla20, the DIS and IRI are closely related to the molecular weight as the ice control property is enhanced at high molecular weight. Compared with PMeto48-b-PAla20, the corresponding random copolymer, P(Meto48-co-Ala20), shows weaker ice control activity. Meanwhile, low-field nuclear magnetic resonance results show that the polymer–water interaction increases with hydrophobic block ratio, but has less effect on the molecular weight. The ice control activity is probably related to the balance between the polymer–water interaction and the polymer–ice interaction, and is optimized by both strong interactions.
研究领域:
生物化学
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材料科学
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低温生物学
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产品关联
DOI
PubMed
高影响因子
Gels
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2024
·Vol. 10
·期 12
·808
·
IF 5
Feng Li,Ruoxi Yang,Jian Xu,Guohai Xu,Ye Wu
The concentration of small molecules reflects the normality of physiological processes in the human body, making the development of simple and efficient detection equipment essential. In this work, inspired by a facile strategy in point-of-care detection, two devices were fabricated to detect small molecules via photocurrent measurement. A linear response of the photocurrent against the concentration of the small molecules was found. In the first device, metal ions were introduced into gel substrates made by xanthan gum to enhance photocurrent response. N-phenyl-2-naphthylamine was measured when iron or manganese ions were used. L-Phenylalanine was measured when the gel was modified by samarium, iron, cerium, or ytterbium ions. L-(+)-Arabinose was detected via the gels modified by iron or holmium ions. D-(+)-Mannose was detected when the gel was modified by ytterbium, manganese, chromium, or sodium ions. L-Methionine was detected in the gels modified by samarium, zinc, or chromium ions. The second device was based on a paper sheet. A sugar-like molecule was first synthesized, which was then used to modify the paper. The detection was possible since the photocurrent showed a linear trend against the concentration of D-Trehalose. A linear fit was conducted to derive the sensitivity, whose value was found to be 5542.4. This work offers a novel, simple, and environmentally sustainable platform that is potentially useful in remote areas lacking medical devices.
研究领域:
生物医学工程
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分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Microorganisms
·
2024
·Vol. 12
·期 2
·360
·
IF 4.1
Shengwang Li,Youwei Yu,Peng Xie,Xianran Zhu,Chao Yang,Linjing Wang,Shaoying Zhang
Gray mold caused byBotrytis cinereais a common postharvest fungal disease in fruit and vegetables. The prevention and treatment of postharvest gray mold has been one of the hot research issues addressed by researchers. This study aimed to investigate the effect of L-methionine and L-arginine onBotrytis cinereain vitro and on cherry tomato fruit. The results of the in vitro experiment showed that L-methionine and L-arginine had significant inhibitory effects on the mycelial growth and spore germination ofBotrytis cinerea, and the inhibitory effects were enhanced with increasing L-methionine or L-arginine concentration. In addition, L-methionine and L-arginine treatment increased the leakage ofBotrytis cinereaelectrolytes, proteins and nucleic acids. The experiment involving propidium iodide staining and malondialdehyde content assay also confirmed that L-methionine and L-arginine treatment could lead to cell membrane rupture and lipid peroxidation. The results of scanning electron microscopy further verified that the morphology of hyphae was damaged, deformed, dented and wrinkled after treatment with L-methionine or L-arginine. Fruit inoculation experiments displayed that L-methionine and L-arginine treatments significantly inhibited the occurrence and development of gray mold in postharvest cherry tomato. Therefore, treatment with L-methionine or L-arginine might be an effective means to control postharvest gray mold in fruit and vegetables.
研究领域:
植物病理学
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食品科学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Hang Su,Shuo Huang,Xin Gao,He Bai,Haopeng Zhang,Long Li,Lei Zhao,Hongyan Yue
Highly efficient recognition and detection of chiral molecules with similar structures are of great significance in the fields of medicine and biochemistry. In this study, strobili fibers of the plane tree were carbonized at high temperatures to prepare hollow chiral helical carbon tubes (HCT). Subsequently, l-threonine (l-Thr) was nanoassembled on the HCT surface (l-Thr@HCT) through hydrogen bonding and electrostatic interactions. Benefiting from the synergistic effect of conductive HCT with inherent chirality and l-Thr with abundant chiral sites, the electrode demonstrates a satisfied recognition efficiency of 3.20 for the cysteine (Cys) enantiomer. The l-Thr@HCT/GCE electrode exhibits a lower affinity toward l-Cys than d-Cys, thereby facilitating the diffusion of l-Cys to the electrode surface and exhibiting excellent selectivity for l-Cys. Under the optimal conditions, the electrode has a high sensitivity of 1.74 μA·mM–1 for l-Cys with a low detection limit of 8.58 μM (S/N = 3). Moreover, the electrode has good repeatability, reproducibility, and stability. This electrode will have a potential clinical application in chiral sensing.
研究领域:
生物医学工程
·
分析化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Ruichang Gao,Tong Shi,Quancai Sun,Xiuting Li,David Julian McClements,Li Yuan
This research focused on the effects of l -arginine ( l -Arg) and l -histidine ( l -His) on the heat-induced aggregation of fish myosin. l -Arg/ l -His increased the pH of the myosin solution from 6.82 to 8.74 and 7.24, respectively, and decreased the turbidity, aggregate size, shear modulus, and breaking force. The incorporation of l -Arg/ l -His decreased the surface hydrophobicity during setting, but increased it during the two-step heating. The heat-induced aggregation of myosin was suppressed by both amino acids, with the inhibitory effect being greater for l -Arg than l -His. On one hand, the change in the pH played a critical role in suppressing the heat-induced aggregation of myosin. On the other hand, the characteristics of l -Arg/ l -His themselves, such as net charges and particular R-groups, were another main contributor to aggregation suppression. Particularly, l -Arg/ l -His could interact with exposed aromatic residues of myosin, and the interactions may dominate and overwhelm the burial of aromatic residues during two-step heating.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2025
·
IF 5.5
Hang Su,Shuo Huang,Dewen Su,Carlos Garcia Nuñez,Hadi Heidari,Haopeng Zhang,Jingming Lan,Xin Gao,Hongyan Yue
It is highly important to efficiently recognize and detect chiral molecules in drug development and life sciences. In this paper, an electrochemical chiral sensor for the detection of L-tryptophan (l-Trp), based on l-cysteine (l-Cys)@island-like Co3S4 on Co3O4 nanosheet arrays heterostructure/carbon paper (l-Cys@Co3S4–Co3O4/CP), is presented. Co-MOF triangular nanosheets with a thickness of ∼200 nm are first grown on the CP. After calcination and partial sulfidation, the Co3S4–Co3O4 heterostructure forms. Finally, l-Cys, as a chiral selector, is uniformly decorated on the heterostructure. The island-like heterostructure exhibits superior electrical conductivity and could potentially provide active sites for the absorption of l-Cys. The l-Cys@Co3S4–Co3O4/CP electrode is utilized for the electrochemical recognition and detection of L-tryptophan (l-Trp) via a differential pulse voltammetry (DPV) method. It demonstrates a recognition efficiency of 1.50 and shows high sensitivity (0.33 μA·μM–1) and a low detection limit (0.021 μM) for l-Trp within the concentration range of 0.06–2 mM. The electrode has good anti-interference ability, reproducibility, and repeatability. Moreover, it exhibits good detection performance of l-Trp in biological samples, with recovery ranging from 96% to 105%, demonstrating excellent potential in clinical applications.
研究领域:
分析化学
·
生物医学工程
·
材料科学
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产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2021
·
IF 6.901
Xiaofeng Wei,Ying Chen,Shan He,Huiting Lian,Xuegong Cao,Bin Liu
Metal-organic framework (MOF)-based electrochemical interface presents a broad platform for the chiral recognition of amino acids , but the inferior conductivity of most MOFs limits the sensitivity in practical applications of electrochemical sensing. Therefore, the L-histidine-regulated zeolitic imidazolate framework (L-His-ZIF) was proposed as a novel electrochemical interface for simple, efficient, and enantioselective determination of L-glutamate (L-Glu) based on the electrochemical impedance spectroscopy (EIS). Based on the resistance change, the L-His-ZIF interface can be directly used for chiral recognition without the assistance of high-conductive substances, where multiple immobilizations are generally required in MOF-involved voltammetry . The stacked L-His-ZIF nano-polyhedrons provided numerous cavities and high specific surface area, thereby forming adequate chiral recognition sites. Based on the L-His-ZIF interface, the enantioselective detection of l -Glu-was successfully achieved with a good linear relationship with the impedance difference in the range from 0.1 nM to 50 nM with the detection limit of 0.06 nM. This prepared ZIF-functionalized electrode provided the desired enantioselectivity and reproducibility with robust chiral recognition in the real application. More importantly, MOF-modified EIS can be applied for more chiral recognition of enantiomers by various MOF interfaces, which has great potential for applications in pharmaceutical monitoring and biochemical analysis.
研究领域:
分析化学
·
生物医学工程
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）