ANALYST
·
2024
·
IF 3.6
Yating Rui,Qunyong Tang,Liyi Chen,Juan Pu,Wangpeng Wang,Shou-Nian Ding
Baijiu, as a traditional Chinese alcoholic beverage, carries China's rich historical and cultural heritage. Consumers experience varying levels of relaxation and pleasure after consuming different Baijiu, with the biological basis of delectation influenced by serotonin and dopamine. In this study, we prepared carbon fiber electrodes modified with surface decorated gold nanoparticles to directly measure the electrochemical response signals in the serum of mice before and after gavage different types of Baijiu. It was observed that the serum signal change in mice after consuming Baijiu Sample 1 (J1) was higher than the other two types of Baijiu. Consequently, trace flavor compounds in the Baijiu samples were detected using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography mass spectrometry (LC-MS), revealing the highest content of L-lactic acid in J1. Mice were intraperitoneally injected with 200 mg/kg of L-lactic acid. The changes in dopamine and serotonin in the serum of injected mice were monitored using a biosensor, and the results were compared with results from high performance liquid chromatography-triple quadrupole mass spectrometry (HPLC-MS). The findings confirmed that L-lactic acid could indeed stimulate both neurotransmitters' secretion in mice, suggesting that the trace components in J1 may even exhibit synergistic effects. The study contributes to a deeper under-standing of the effects of Baijiu on body and provides a scientific basis for the production and consumption of Baijiu.
研究领域:
生物化学
·
神经科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHARMAZIE
·
2020
·Vol. 75
·期 10
·483-487
·
IF 1.198
Wang Lili,Liu Shuyu,Gao Ziyao
Berberine hydrochloride (BCl) is commercially used to treat diarrhea, diabetes, hyperlipidemia, and cancer. However, BCl suffers from solid state instability, low aqueous solubility, low dissolution rate, and poor bioavailability, which limit its potential application in clinical medicine.
In this work, we report a novel cocrystal hydrate of BCl with L(+)-lactic acid (BCl-LA-H2O), designed to improve its physicochemical properties, thus promoting its application in the pharmaceutical industry. As a result, the cocrystal strategy improved the solubility, dissolution,
melting point, and hygroscopicity of BCl, which indicated that the BCl-LA-H2O can be used as a better solid form.
研究领域:
药学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2014
·
IF 2.045
Qinghua Luan,Yongli Wang,Guan Wang,Jingxiang Yang,Hongxun Hao
The solubility of calcium-l-lactate pentahydrate (CLLP) in binary ethanol + water and acetone + water solvent mixtures was measured in the temperature range of 278.15 K to 313.15 K by using a synthetic method. The solubility of CLLP increases with increasing temperature, whereas it decreases with the increase of the initial mole fraction of ethanol or acetone. The solubility data were correlated by using the modified Apelblat equation, the CNIBS/R-K equation, and the hybrid model, respectively. It was found that the modified Apelblat equation could give better correlation results both in ethanol aqueous solutions and in acetone aqueous solutions. The dissolution enthalpy, entropy, and Gibbs energy change of dissolution of CLLP in these solvent mixtures were obtained by using the modified van’t Hoff equation.
研究领域:
化学工程
·
热力学
✓ 已验证
DOI
高影响因子
GREEN CHEMISTRY
·
2014
·Vol. 16
·期 4
·2234-2241
·
IF 6.852
Qiaohong Peng,Khalid Mahmood,Ying Wu,Leli Wang,Yanyan Liang,Jianan Shen,Zhengping Liu
Biodegradable copolymers of L-lactic acid and ε-caprolactone [P(LLA-CL)s] were prepared in sulfonic acid-functionalized Brønsted acidic ionic liquids (SFBAILs) with very different anions and cations. SFBAILs work as both solvents and catalysts. The copolymerization process was proved to be a combination of polycondensation and simultaneous transesterification after a fast ring-opening polymerization of ε-caprolactone through a kinetic study. Copolymers with different monomer ratios were obtained. We found that the anions rather than the cations of SFBAILs have a great impact on the polymerizations. In addition, SFBAILs can be simply recovered by phase separation and recycled without decreasing the efficiency.
研究领域:
高分子化学
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Systems Microbiology and Biomanufacturing
·
2021
·Vol. 2
·期 3
·473-486
·
IF 0
Wu Aiai,Bai Yajun,Fan TaiPing,Zheng Xiaohui,Cai Yujie
l -Lactate dehydrogenases can reduce alpha-keto carboxylic acids asymmetrically and generally have a broad substrate spectrum. l -Lactate dehydrogenase gene (LF- l -LDH0845) with reducing activity towards 3,4-dihydroxyphenylpyruvate and phenylpyruvate was obtained from Lactobacillus fermentum JN248. To change the substrate specificity of LDH0845 and improve its catalytic activity towards large substrates, site-directed mutation of Tyr221 was performed by analyzing the amino acids in the active center. Kinetic parameters show that the k cat values of Y221F mutant on 3,4-dihydroxyphenylpyruvate, 4-methyl-2-oxopentanoate, and glyoxylate are 1.21 s −1 , 1.35 s −1 , and 0.72 s −1 , respectively, which are 420%, 150% and 130% of the wild-type LDH0845. This study shows that the mutations of Y221 can significantly change the substrate specificity of LDH0845, making it become a potential tool enzyme for the reduction of alpha-keto carboxylic acids with large functional groups.
研究领域:
生物化学
·
分子生物学
·
酶工程
✓ 已验证
DOI
Polymers
·
2021
·Vol. 13
·期 8
·1270
·
IF 4.329
Mengyuan Zhang,Zhonghua Chang,Xiaofeng Wang,Qian Li
Biodegradable and bioabsorbable polymers have drawn considerable attention because of their mechanical properties that mimic human soft tissue. Poly(l-lactide-co-ε-caprolactone) (PLCL), the copolymer of L-lactic (LA) and ε-caprolactone (CL), has been applied in many tissue engineering and regenerative medicine fields. However, both the synthesis of PLCL and the structure-activity relationship of the copolymer need to be further investigated to allow tuning of different mechanical properties. The synthesis conditions of PLCL were optimized to increase the yield and improve the copolymer properties. The synthetic process was evaluated by while varying the molar ratio of the monomers and polymerization time. The mechanical properties of the copolymer were investigated from the macroscopic and microscopic perspectives. Changes in the polymerization time and feed ratio resulted in the difference in the LA and CL content, which, in turn, caused the PLCL to exhibit different properties. The PLCL obtained with a feed ratio of 1:1 (LA:CL) and a polymerization time of 30 h has the best toughness and elasticity. The developed PLCL may have applications in dynamic mechanical environment, such as vascular tissue engineering.Keywords:poly(l-lactide-co-ε-caprolactone);synthesis;segment;toughness;elasticity
研究领域:
生物材料学
·
组织工程学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2023
·
IF 5.7
Song Baiyang,Fang Li,Mao Xufeng,Ye Xianwang,Yan Zejun,Ma Qi,Shi Zewen,Hu Yiwei,Zhu Yabin,Cheng Yue
Introduction: The repair of a diseased ureter is an urgent clinical issue that needs to be solved. A tissue-engineered scaffold for ureteral replacement is currently insufficient due to its incompetent bioactivity, especially in long-segment abnormalities. The primary reason is the failure of urothelialization on scaffolds.Methods: In this work, we investigated the ability of gelatin-grafted tubular scaffold in ureteral repairment and its related biological mechanism. We designed various porous asymmetric poly (L-lactic acid) (PLLA)/poly (L-lactide-co-e-caprolactone) (PLCL) tubes with a thermally induced phase separation (TIPS) method via a change in the ratio of solvents (named PP). To regulate the phenotype of urothelial cells and ureteral reconstruction, gelatin was grafted onto the tubular scaffold using ammonolysis and glutaraldehyde crosslinking (named PP-gel). The in vitro and in vivo experiments were performed to test the biological function and the mechanism of the scaffolds.Results and Discussion: The hydrophilicity of the scaffold significantly increased after gelatin grafting, which promoted the adhesion and proliferation of urothelial cells. Through subcutaneous implantation in rats, PP-gel scaffolds demonstrated good biocompatibility. The in vivo replacement showed that PP-gel could improve urothelium regeneration and maintain renal function after the ureter was replaced with an ∼4 cm-long PP-gel tube using New Zealand rabbits as the experimental animals. The related biologic mechanism of ureteral reconstruction was detected in detail. The gelatin-grafted scaffold upgraded the integrin α6/β4 on the urothelial cell membrane, which phosphorylates the focal adhesion kinase (FAK) and enhances urothelialization via the MAPK/Erk signaling pathway.Conclusion: All these results confirmed that the PP46-gel scaffold is a promising candidate for the constitution of an engineered ureter and to repair long-segment ureteral defects.
研究领域:
生物医学工程
·
组织工程
·
泌尿外科
·
细胞生物学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED CATALYSIS A-GENERAL
·
2021
·
IF 5.706
Somboon Chaemchuen,Qingping Dai,Jichao Wang,Chenyang Zhu,Nikom Klomkliang,Ye Yuan,Chen Cheng,Mirella Elkadi,Zhixiong Luo,Francis Verpoort
A catalyst series of zeolitic imidazole frameworks (ZIFs) with a sodalite (SOD) structure was explored as an effective heterogeneous catalyst for l -lactide polymerization into biodegradable polylactides (PLAs). The catalyst series of ZIF-8, ZIF-67, and bimetallic ZnCo-ZIF was synthesized under the green method (room temperature synthesis), which contained a variance in the metal species/contents. The chemical and physical properties of catalysts were characterized by several techniques to illustrate the relation between catalyst properties and catalytic performance. The results indicated that the metals in the ZIF structure revealed an influencing role in catalytic activities, kinetic rates, and properties of polymer products under bulk ring-opening polymerization of l -lactide. Moreover, the best performing ZIF showed potential as a heterogeneous catalyst since it could be reused at least four times without significant loss of catalytic activity.
研究领域:
材料科学
·
催化化学
✓ 已验证
DOI
高影响因子
JOURNAL OF POLYMERS AND THE ENVIRONMENT
·
2022
·Vol. 31
·期 1
·1-12
·
IF 4.705
Yuan Yuka,Fu Zhe,Liu Yudong,Liu Kai,Hua Jing
Poly (L-lactic acid) (PLLA) suggests attractive biomedical applications in drug releasing system, surgical suture, bone fixing and tissue repairing due to its degradable and biocompatible benefits. However, currently developed catalysts with high toxicity and currently reported PLLA with uncontrollable crystallinity are detrimental for the environmental protection and human health. Herein, we proposed a novel and effective approach to synthesizing PLLA using environmentally-friendly bio-based phenol-substituted triisobutylaluminum as the catalyst. The effects of the chemical structures of various bio-based phenols on the polymerization activity and stereoregularity of PLLA were explored in detail. The increased steric hindrance of bio-based phenols was beneficial for the polymerization activity of PLLA. The resultant PLLA exhibited an ultrahigh monomer conversion efficiency up to 98.32% among the highest value of reported PLLA. Moreover, the PLLA also demonstrated quick polymerization rate (< 5 h) and low polymerization temperature (< 100 °C), with controllable polymer crystallinity (36 ~ 46%) and number average molecular weight (8400 ~ 16,000 g mol −1 ). Consequently, the strategy and results collectively represent a significant advance toward the preparation of high-performance degradable and biocompatible PLLA.
研究领域:
材料科学
·
生物医学工程
·
高分子化学
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2014
·
IF 6.451
Ai-Ling Hu,Yin-Huan Liu,Hao-Hua Deng,Guo-Lin Hong,Ai-Lin Liu,Xin-Hua Lin,Xing-Hua Xia,Wei Chen
A novel fluorescent hydrogen peroxide sensor was developed based on the peroxidase-like activity of cupric oxide nanoparticles . Cupric oxide nanoparticles effectively catalyzed the decomposition of hydrogen peroxide into hydroxyl radicals . Then terephthalic acid was oxidized by hydroxyl radical to form a highly fluorescent product. The linear range of hydrogen peroxide estimated to be 5.0×10 −6 –2.0×10 −4 M with a detection limit of 3.4×10 −7 M. Moreover, this detection system enabled the sensing of analytes which can enzymatically generate hydrogen peroxide. By coupling the oxidation of glucose or l -lactate catalyzed by their corresponding oxidase enzymes with terephthalic acid oxidation catalyzed by cupric oxide nanoparticles, sensitive assays of glucose and l -lactate with detection limits of 1.0×10 −6 and 4.5×10 −8 M were realized. The successful applications of this approach in human serum samples have also been demonstrated.
研究领域:
生物化学
·
医学检测
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2014
·Vol. 140
·期 3
·797-802
·
IF 3.906
Chong Sun,Daoying Wang,Muhan Zhang,Yanxiu Ni,Xiaohui Shen,Youchao Song,Zhiming Geng,Weimin Xu,Fang Liu,Chun Mao
The development of advanced nanomaterials for the highly efficient electrical detection of biological species has attracted great attention. Here, novel polypyrrole-Pluronic F127 nanoparticles (PPy-F127 NPs) with conducting and biocompatibility properties were synthesized and used to construct a L-lactic acid biosensor that could be applied in biochemical assays. The PPy-F127 NPs were characterized by transmission electron microscopy (TEM), elemental analysis and UV-vis spectroscopy. Lactate oxidase (LOx) structure variation on the PPy-F127 NPs was investigated by circular dichroism (CD). The cyclic voltammetric results indicated that LOx immobilized on the PPy-F127 NPs exhibited direct electron transfer reaction with a formal potential value (E0′) of 0.154 V vs. SCE. Moreover, the biosensor had good electrocatalytic activity toward L-lactic acid with a wide linear range (0.015–37.5 mM) and a low detection limit of 0.0088 mM. The regression equation was I (μA) = 0.02353c (mM) + 1.4135 (R2 = 0.9939). The L-lactic acid biosensor had a good anti-interference property towards uric acid (UA), ascorbic acid (AA), glucose and cysteine. The idea and method provide a promising platform for the rapid development of biosensors that can be used in the detection of biological species.
研究领域:
生物化学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2012
·Vol. 19
·期 12
·1-8
·
IF 1.733
Ma Chi,Wang Bing,Xiong Cheng-Dong,Chen Dong-Liang,Xiong Zuo-Chun
Control of dimensional stability is crucial for using electrospun membranes in biomedical and tissue engineering applications. In this study, the contraction of electrospun membranes of poly( l -lactide-co-glycolide) (PLLGA) after stretch was investigated. Then, by adding PLLGA-b-polyethylene glycol (PLLGA-PEG), the contraction of the membranes was controlled. In addition, the mechanical properties of the membranes were investigated. On the basis of the strain–stress behavior of the membranes, an elongation of 50 % was used to investigate the orientation degrees of the membrane fibers. On the basis of the morphology of the stretched membranes, the contraction was found to depend on the orientation degree of fibers after stretch. A linear relationship between the contraction and degree of orientation after stretch was confirmed. Two-dimensional fast Fourier transform (2D FFT) analysis of the scanning electron microscopy (SEM) images of the membranes was used to quantify the orientation degree. It was surmised that the different orientation degrees were responsible for the different morphologies and thermal behaviors of the membranes.
研究领域:
生物医学工程
·
组织工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）