OPTICAL MATERIALS
·
2025
·
IF 4.2
Chenyan Lu,Dajie Zou,Yanyan Jia,Yongchang Lu,Huan Wang,Yanfeng He
In this study, a novel nitrogen-chlorine-codoped carbon quantum dots (NCl-CQDs) with low toxicity, thermal stability, good fluorescence properties, biocompatibility and high sensitivity was successfully prepared by hydrothermal carbonization. Based on the above advantages of NCl-CQDs, the NCl-CQDs was used to construct fluorescence sensing system for fluorescence probes. The maximum fluorescence emission wavelength was obtained at 470 nm when the excitation wavelength was 390 nm. The fluorescence signal was in the state of “ON”. The introduction of 2,6-Dichloroindophenol Sodium (DCIP) resulted in the quenching of fluorescence intensity due to photoinduced electron transfer between DCIP and NCl-CQDs. At this point, the color of the solution changed from brownish-yellow to light blue, indicating that the fluorescence signal was in an “OFF” state. Upon addition of ascorbic acid (AA) to the NCl-CQDs/DCIP system, the corresponding fluorescence signal was restored due to the special interaction between AA and DCIP. The color of the corresponding solution changed from light blue to colorless. The fluorescence signal was in the “on” state. Therefore, an “ON-OFF-ON” sensing system and colorimetric sensing system for AA was constructed using the NCl-CQD fluorescence probe. Under the optimized experimental conditions, the proposed methods exhibited satisfactory linear ranges. Specifically, the linear range for AA detection by the fluorescence sensor spanned from 1.2 μM to 180.33 μM, while that by the colorimetric sensor ranged from 1.32 μM to 166.55 μM. The corresponding detection limits were determined to be 0.68 μM and 0.55 μM, respectively. In summary, this sensing system could effectively enhance selectivity, sensitivity, and accuracy by preventing false positive detection results caused by environmental and system errors.
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高影响因子
LUMINESCENCE
·
2026
·Vol. 41
·期 2
·e70439
·
IF 2.8
Yuxun Zhao,Qiuhua Wu,Lu Bai,Chenghuan Wang,Guolin Zhang,Wenxiao Yang
Blue fluorescent carbon dots (CDs) were synthesized in this study from poly (butylene adipate co -terephthalate) (PBAT) and poly (ethylene terephthalate) (PET) waste through a sequential carbonization and hydrothermal treatment. The nanoparticles exhibited a spherical morphology with a narrow size distribution of 1.3–2.5 nm, abundant hydrophilic surface groups, and excellent dispersibility in aqueous solution. The CDs exhibited strong blue fluorescence with a quantum yield (QY) of 7.4% and remarkable stability. The fluorescence was selectively quenched by gefitinib primarily via a Förster resonance energy transfer (FRET) mechanism. The developed sensor showed a linear detection range of 0–30 μM, a low detection limit of 0.8433 μM, and outstanding anti-interference capability against common biomolecules and structurally similar drugs. The spiked recovery rates in real samples ranged from 96.7% to 103.0%, with the relative standard deviations (RSDs) less than 3.0%, demonstrating the probe's capability for detecting gefitinib in complex practical matrices. The proposed sensor shows great potential for applications in therapeutic drug monitoring and clinical diagnostics, offering a promising solution for both environmental sustainability and analytical science.
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Food Bioscience
·
2026
·
IF 6.2
Yingying Zeng,Weiwei He,Yuhao Li,Junyi Yin,Mingyong Xie
Fermented celery puree is valued for its low caloric content and health benefits. However, its characteristic grassy flavor often limits consumer acceptance. This study examined how supplementation with four amino acids (i.e. valine, leucine, isoleucine, and particularly phenylalanine) modulated the fermentation of celery puree by three lactic acid bacteria (LAB) strains, including Lactiplantibacillus plantarum NCU116, L. casei NCU215, and L. acidophilus NCU402. Among all fermentations, phenylalanine supplementation showed the most pronounced enhancement in both metabolic activity and aroma formation. Amino acid addition accelerated acidification and microbial growth in NCU116 and NCU215 fermentations, whereas its influence on NCU402 was minimal. Phenylalanine supplementation markedly enhanced floral and fruity volatiles, elevating phenylacetaldehyde (∼23-fold), phenylethyl alcohol (∼3-fold), linalool (∼2-fold), and 1,4-dimethyl-4-acetyl-1-cyclohexene (∼1.25-fold). In contrast, valine, isoleucine, and leucine showed only minor effects, mainly on energy metabolism and overall volatile balance. Metabolomic pathway enrichment revealed activation of the phenylpropanoid and shikimate pathways by phenylalanine, indicating enhanced aromatic metabolism, whereas the other amino acids mainly modulated glycolysis/TCA-linked energy metabolism. These findings indicate that phenylalanine acts as an important exogenous precursor and key contributor to aromatic metabolism and volatile formation in NCU215-fermented celery puree, providing mechanistic insights and a practical strategy to enhance sensory acceptance and functional quality of plant-based fermented foods.
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PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2026
·
IF 6.2
Qiuping Li,Wenqing Ma,Yuanfeng Zhang,Yandong Sun,Qijiao Xiong,Yu Yong,Yitong Shi,Lijuan Chen,Jiancheng Wen,Dandan Li
Low-temperature (LT) stress severely limits rice growth and productivity, particularly in the chilling-sensitive indica subspecies. Here, we investigated the physiological, transcriptomic, and metabolomic responses of two similar genetic backgrounds indica rice varieties but contrasting cold tolerance: Diantun502 and Diantun506. Under cold stress, Diantun502 exhibited higher survival rates, greater chlorophyll retention, and elevated accumulation of osmoprotectants, branched-chain amino acids (BCAAs), and stress-related phytohormones compared to Diantun506. Integrative multi-omics analysis revealed that amino acid metabolism, BCAA-related pathways, was prominently enriched among differentially expressed genes and metabolites. We identified OsDIAT ( Os05g0244700 ), encoding a branched-chain amino acid aminotransferase (BCAT), as a key candidate gene. Sequence variation in Diantun506 introduced a premature stop codon and generated a truncated protein with negligible catalytic activity. In an F 2 population derived from Diantun502 × Diantun506, cold-tolerance phenotypes were closely associated with OsDIAT genotypes, supporting the contribution of the Diantun506 allele to reduced LT tolerance. Overexpression of OsDIAT enhanced LT tolerance, BCAT activity, BCAA accumulation, and stress-related hormone levels, whereas knockout produced the opposite effects. Exogenous isoleucine partially restored the survival of knockout plants and promoted JA-Ile accumulation, the JA-Ile/JA ratio, and OsJAR1 expression. Collectively, these findings demonstrate that OsDIAT enhances LT tolerance by maintaining BCAA homeostasis and supporting associated JA-Ile responses, and provide a useful genetic target and molecular marker for breeding cold-tolerant indica rice.
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高影响因子
TALANTA
·
2026
·
IF 6.7
Zifeng Wang,Cong Dai
The dual-channel fluorescent sensing of hydroxyl radicals (·OH) is of great importance for environment and life. Organic cage for fluorescence sensing suffers from short excitation/emission wavelength and poor water solubility, while the application of carbon quantum dots is also hindered by the time-consuming preparation in high pressure and temperature conditions. Herein, we report a red emitted carbon dots derived from cationic organic cage for dual-channel fluorescent sensing of ·OH. The abundant flexible methylene within the cationic organic cage leads to remarkable collapse of intrinsic cavity. The ring-opening of imidazole structure occurs via redox reaction at acidic condition, resulting in the simple formation of carbon dots with the emission at 640 nm. Under acidic conditions, the ·OH generated via Fenton reaction exhibits strong affinity toward the prepared carbon dots. The mild oxidation at pH 1 significantly enhances the fluorescence, whereas the severe oxidative degradation at pH 6 leads to obvious fluorescence quenching. Based on the mutual verification of dual signals, a dual-channel fluorescent sensing method is established for ·OH in environmental samples and biological fluids. The sensing at pH 6 and 1 respectively achieves the linearity range of 1-50 and 5-50 μM, the limit of detection of 7.3 and 11.8 nM, and the relative standard deviation of 0.76% and 0.83% for 10 replicate analysis. The proposed method gives the recoveries of spiked ·OH ranged from 97.3% to 103.4% in real samples, demonstrating excellent accuracy and reliability. This study underscores the promising application potential of organic cage and carbon dots in fluorescent sensing. Furthermore, combined with Fenton reaction, the proposed dual-channel sensing platform can also be utilized to the detection of hazardous substances or biomarkers capable of H 2 O 2 generation.
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Foods
·
2026
·Vol. 15
·期 17
·3047
·
IF 6
Yi-Jie Liu,Jia-Ying Chen,Mei-Juan Hu,Jian-Hui Ye,Wen-Wen Luo
To elucidate the chemical basis underlying bitterness and astringency modulation in jasmine tea, four green tea bases and their corresponding jasmine teas were analyzed using weighted gene co-expression network analysis (WGCNA) combined with spiking validation experiments. WGCNA revealed that catechin, gallocatechin gallate, aspartic acid (Asp), glutamic acid (Glu), arginine (Arg), and alanine (Ala) were positively associated with sweetness and umami but negatively associated with bitterness and astringency. Spiking experiments showed that taste modulation was both concentration- and matrix-dependent. Glu and Asp effectively reduced bitterness at suitable concentrations, whereas the effects of theanine and Arg varied among tea matrices. Bitterness and astringency of jasmine tea infusion are modulated by the combined effects of the chemical matrix of tea base, providing a theoretical basis for tea-base selection and jasmine tea flavor optimization.
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高影响因子
MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 9
·649
·
IF 5.4
Cai Chuangui,Lin Zhen,Yao Wensong,Lian Shan,Huang Kaiyuan,Deng Haohua,Lin Xinhua,Chen Wei
Arginine surface-engineered 6-aza-2-thiothymine gold nanoclusters (Arg/ATT-AuNCs) via host-guest assembly were synthesized which exhibit exceptional catalytic activity and intense photoluminescence. Arg/ATT-AuNCs enhanced the CL intensity from NaIO 4 -H 2 O 2 system by 1200-fold. The CL mechanistic studies revealed that Arg/ATT-AuNCs not only accelerated decay of H 2 O 2 to form reactive oxygen species, but also acted as efficient energy acceptors of singlet oxygen ( 1 O 2 ) via a CL resonance energy transfer (CRET) pathway. It was observed that doxorubicin (DOX) quenches the CL signal through an inner filter effect that disrupts the CRET process. Based on this, a straightforward, rapid, and highly sensitive biosensor has been developed for the detection of DOX in human serum samples, achieving a low detection limit of 0.029 µM. The biosensor was applied to DOX determination in human serum samples with recoveries ranging from 91.1 to 106.2%. This study not only provides a new method for the detection of DOX, but also broadens the application scope of AuNCs in the field of CL. Graphical
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MOLECULAR CELL
·
2026
·Vol. 86
·期 4
·722-739
·
IF 16
Jie Du,Chaoqun Li,Li Chai,Fabao Zhao,Lin Lv,Zongcheng Yang,Zhiyuan Zhao,Rong Gong,Liu Yang,Meng Wu,Meng Nie,Jihui Jia,Dongwei Kang,Chengjiang Gao,Wei Zhao,Mutian Jia
Competition between the host and invading viruses for cellular nutrients determines the outcomes of infectious diseases. Nutrients are increasingly being recognized as regulators that interact with immunological signals, but how immune cells sense specific nutrients to regulate antiviral innate immune responses remains elusive. Here, we establish asparagine (Asn) as an intercellular nutritional checkpoint that is sensed by TANK-binding kinase 1 (TBK1) to drive innate immune responses in human and murine cells. Mechanistically, Asn directly binds to TBK1, which robustly induces TBK1 phase separation and forms liquid-like droplets, promoting TBK1 transautophosphorylation and activation. Moreover, viral infection reduces asparagine synthetase (ASNS) expression to establish an Asn-restricted microenvironment, thereby evading TBK1-triggered host immune defenses. Overall, our results suggest that TBK1 is a natural Asn sensor that links host nutritional homeostasis to antiviral immune responses and reveal that targeting Asn availability is a promising therapeutic strategy for diseases involving dysregulated TBK1 activation.
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ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Simin Zhang,Xiangfeng Chen,Yaping Shi,Xiangyu Zhu,Zongwei Cai
As bioactive compounds, chiral amino acids (AAs) have been demonstrated to play pivotal roles in different physiological processes, thereby influencing human health. The assessment of the relative enantiomeric ratio through analytical methods is essential for indicating the abnormal status of disease progression, which drives the development of effective methods for resolving and identifying enantiomers. This work presents a robust strategy for the ultrahigh-resolution analysis of chiral AAs using MALDI-TIMS-MS. By employing a chiral derivatization process using S-NIFE and stabilizing the soft chain of the derivatization reagent with bimetal ion addition, the separation of 19 pairs of proteinogenic AAs was successfully achieved. This method not only demonstrates outstanding separation capability but also proves its efficiency in determining enantiomeric ratios, extending its applicability to real sample analysis.
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高影响因子
DYES AND PIGMENTS
·
2022
·
IF 5.122
Yujiao Tu,Ze Liu,Lei Jiang,Yingying Xiang,Fei Song,Lifen Meng,Xiuling Ji,Lin Yuan
New coccine is one of the most used red colorants in foods. Due to its cytotoxicity, quantitative determination is very necessary. Herein, the ratiometric fluorescent and UV absorption dual-mode nanosensor , derived from fangchinoline and o-phenylenediamine, was successfully prepared by hydrothermal way. The prepared carbon dots with three colors were purified with dialysis, thin layer chromatography and chromatographic column. Highly green fluorescence of Fan,N-CDs was quenched by new coccine with inner filter effect. The dependence of the ratiometric fluorescent response on new coccine concentrations was found linear over the range of 0–12.5 μM and 20–55 μM with LOD of 30.12 nM and 54.85 nm, respectively. The linearity of UV absorption ranged from 0 to 45 μM with of LOD 1.98 nM. In addition, the nanosensor was used to determine new coccine in energy drinks, carbonated beverage, gum and ketchup. It provides a new insight on the detection of new coccine by the dual-mode nanosensor.
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高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Lei Di,Shengyu Cheng,Yan Zhu,Yueying Jin,Chao Qi,Lingli Zhang,Minghui Zhang,Xin Wang,Yu Han,Xi-Ling Li,Jun Zhe Min
We developed a novel chiral mass spectrometry derivatization reagent (S)-(3-(4-carboxythiazolidin-3-yl)-3-oxopropyl) diphenylsulfonium (CTOD) with a positively charged sulfur-containing structure for high-sensitivity detection of the chiral resolution of amino acid enantiomers. CTOD reacted with DL-amino acids at 60 o C for 60 min to generate the corresponding diastereomers, fifteen chiral amino acid-derived products were separated. Resolution (Rs) values were of the range 1.54-4.36, except Asn 1.07, achieving good separation. A highly sensitive and selective UHPLC-MS/MS method for the simultaneous determination and chiral separation of five chiral amino acids (Pro, Ala, Glu, Asp, and Phe) based on CTOD derivatization was established and applied to the detection of chiral amino acids in different wines. The diastereomeric resolution of the five amino acids was 1.71–5.42, and an excellent linear relationship was obtained in the range of 0.25–500 pmol (R 2 ≥0.9993). The detection limit was 0.05–0.25 pmol. The intra- and inter-day precisions were 0.51–5.76% and 0.78–5.18%, respectively, and the average recovery was 90.03–99.99%. In addition, the metabolic concentration of chiral amino acids was monitored after drinking red wine and white wine, and the fitting curve of metabolic concentration was drawn.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Chengxiang Chu,Xianfeng Wang,Yuanyi Deng,Yi Ma,Chengyue Zou,Mei Yang,Huibo Luo,Danqun Huo,Changjun Hou
A facile and rapid fluorescence sensor array based on Tb (III) and Eu (III) doped Zr (IV) metal–organic frameworks was proposed for Chinese green tea discrimination. According to large porosity of Tb@UiO-66-(COOH) 2 and Eu@UiO-66-(COOH) 2 , phenolic hydroxyl groups of tea polyphenols could coordinate with free carboxylic acid groups and was captured into the pores, which led to the disturbance of electronic structure of ligand and inhibited the energy transfer efficiency from ligand to Tb (III) and Eu (III) center, causing the fluorescence quenching effect. Based on Hierarchy Cluster Analysis and Linear Discrimination Analysis, the fluorescence sensor array was employed for successful tea polyphenols classification through the analysis of different fluorescence quenching effect to tea polyphenols. Green tea samples within different categories and grades were also successfully discriminated using this assay according to tea polyphenols, providing a new method for Chinese green tea identification.
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