APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2020
·Vol. 192
·期 3
·935-951
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IF 2.277
Guo Zongren,Long Liangkun,Ding Shaojun
L-Ribulose and L-ribose are two high-value unnatural sugars that can be biosynthesized by sugar isomerases. In this paper, an L-arabinose isomerase (BvAI) from Bacillus velezensis CICC 24777 was cloned and overexpressed in Escherichia coli BL21 (DE3) strain. The maximum activity of recombinant BvAI was observed at 45 °C and pH 8.0, in the presence of 1.0 mM Mn 2+ . Approximately 207.2 g/L L-ribulose was obtained from 300 g/L L-arabinose in 1.5 h by E. coli harboring BvAI. In addition, approximately 74.25 g/L L-ribose was produced from 300 g/L L-arabinose in 7 h by E. coli co-expressing BvAI and L-RI from Actinotalea fermentans ATCC 43279 (AfRI). This study provides a feasible approach for producing L-ribose from L-arabinose using a co-expression system harboring L-Al and L-RI.
研究领域:
生物技术
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生物化学
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微生物学
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DOI
PubMed
高影响因子
AMB Express
·
2019
·Vol. 9
·期 1
·1-11
·
IF 2.226
Guo Zongren,Long Liangkun,Ding Shaojun
d -Mannose and l -ribose are two important monosaccharides, which have attracted public attention recently because of their great application potentials in food, cosmetic and pharmaceutical industries. Sugar isomerases catalyze the sugar isomerization and therefore can be used as the biocatalysts for production of the high-value sugars from inexpensive sugars. l -arabinose isomerase catalyzes the conversion of l -arabinose to l -ribulose, while d -lyxose isomerase catalyzes l -ribulose and d -fructose to l -ribose and d -mannose, respectively. In this paper, a putative d -LI from Bacillus velezensis (BvLI) was identified, characterized and used to produce d -mannose and l -ribose from d -fructose and l -arabinose, respectively. The recombinant BvLI exhibited a maximum activity at 55 °C and pH 6.5, in the presence of 0.1 mM Co 2+ . Approximately 110.75 g/L d -mannose was obtained from 500 g/L d -fructose in 6 h by the recombinant BvLI, and approximately 105 g/L l -ribose was obtained from 500 g/L l -arabinose in 8 h by the successive biocatalysis of l -arabinose isomerase from Bacillus licheniformis (BlAI) and BvLI.
研究领域:
生物化学
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生物技术
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食品科学
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制药学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yuanqi Duan,Zhengyu Hu,Long Jin,Tieqiang Zong,Xiaohui Zhang,Yanan Liu,Pengcheng Yang,Jinfeng Sun,Wei Zhou,Gao Li
The degradation of Belamcanda chinensis (L.) DC. polysaccharides was carried out by five concentrations of trifluoroacetic acid (TFA) (1–5 mol/L), and their physicochemical properties, degradation kinetics and anticomplementary activity were investigated. The findings revealed a notable reduction in the molecular weight of BCP, from an initial value of 2.622 × 10 5 g/mol to a final value of 6.255 × 10 4 g/mol, and the water solubility index increased from 90.66 ± 0.42 % to 97.78 ± 0.43 %. The degraded polysaccharides of B. chinensis exhibited a comparable monosaccharide composition comprising Man, GalA, Glc, Gal, and Ara. As the concentration of TFA increased, the degradation rate constant increased from 1.468 × 10 −3 to 5.943 × 10 −3 , and the process followed the first-order degradation kinetic model (R 2 > 0.97) and the random fracture model (R 2 > 0.96). Furthermore, the five degraded polysaccharides still exhibit good thermal stability. In vitro experiments showed that DBCP-3 exhibited more potent anticomplementary activity than the original polysaccharides and positive drugs, which was strongly correlated with its Mw ( r = 0.6–0.8), inhibiting complement activation by blocking C2 and C4. These results indicated that TFA degradation has a positive effect on polysaccharides, of which DBCP-3 is expected to treat diseases involving hyperactivation of the complement system.
研究领域:
植物化学
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免疫学
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药理学
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DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Xiaohui Zhang,Zhengyu Hu,Yuanqi Duan,Yuxin Jiang,Weiwei Xu,Pengcheng Yang,Wei Zhou,Jinfeng Sun,Gao Li
The purpose of this study was to optimize the extraction process of polysaccharides from the stems of Panax ginseng C. A. Meyer using different solvents (water, acid and alkali), and investigate their protective effects on PC12 cells against Amyloid-β 1–42 (Aβ 1–42 )-induced cell apoptosis. Three polysaccharides (W-GSP, Ac-GSP and Al-GSP) were prepared and optimized: liquid to material ratio 27:1 mL/g, extraction temperature 89°C, extraction time 2.9 h and 0.3 mol/L HCl/NaOH. The results of characterization indicated that the monosaccharides of W-GSP (703.80 kDa), Ac-GSP (641.38 kDa) and Al-GSP (457.04 kDa) all composed mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose with different molar ratios. Interestingly, W-GSP possessed a lower uronic acid, higher molecular weight, rougher surface and poorer thermal stability, whereby W-GSP exhibited a protective effect against Aβ 1–42 -induced apoptosis in PC12 cells by mediating the JNK signaling pathway, restoring the mitochondrial membrane potential and reducing intracellular ROS levels. This study should be provided the theoretical basis for the study and exploitation of polysaccharides from the stems of Panax ginseng C. A. Meyer.
研究领域:
药理学
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植物化学
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DOI
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 5
·998
·
IF 4.6
Yuanqi Duan,Jinfeng Sun,Yongkang Xue,Weiwei Xu,Yuxin Jiang,Tieqiang Zong,Wei Zhou,Zhengyu Hu,Gao Li
Given that the preparation method of polysaccharides affects the functional properties, four types of acidic polysaccharides (BCP30-1a, BCP50-1a, BCP70-1a, and BCP90-1a) were prepared using the gradient ethanol precipitation method. Then, a series of chemical and instrumental analysis techniques were used to compare structural characteristics and morphology. Neuroprotective effects were explored using OGD/R-induced PC12 cells. The results showed that BCP30-1a, BCP50-1a, BCP70-1a, and BCP90-1a had similar characteristic groups and contained both β-glycosidic and α-glycosidic bonds. Their molecular weights, in descending order, were 198.398 kDa, 184.690 kDa, 184.556 kDa, and 184.217 kDa, respectively. In addition, the four polysaccharides contained different proportions of glycosidic bonds, namely, Manp-(1→, →5)-Araf-(1→, →3)-Galp(or GalAp)-(1→, →4)-Glcp-(1→ and →3,6)-Galp-(1→. BCP30-1a also contained a certain proportion of Galp-(1→, and each polysaccharide had different microscopic characteristics and good thermal stability. Finally, BCP50-1a, BCP70-1a, and BCP90-1a exhibited good cytoprotective effects on PC12 cells based on the OGD/R model. These findings provide a novel regulatory strategy for the functional activity of BCPs and offer scientific evidence supporting application in the research field of ischemic stroke.
研究领域:
生药学
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天然产物化学
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神经药理学
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多糖研究
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细胞生物学
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DOI
PubMed
高影响因子
Scientific Reports
·
2024
·Vol. 14
·期 1
·1-20
·
IF 3.8
Chang Shuaishuai,Lei Xuanhao,Xu Weijia,Guan Feng,Ge Jian,Nian Fuzhao
This study aimed to investigate the structural properties of tobacco polysaccharide (TP) and its mechanism of modulating hyperlipidemia in high-fat diet-induced mice. The structural properties of TP were characterized by FT-IR, 1HNMR, SEM, AFM and thermogravimetric analysis. And the regulatory mechanism of TP on lipid metabolism was investigated in hyperlipidemia mice. These results showed that TP had a high composition of reducing monosaccharide and the glycosidic bond type was α-glycosidic bond. The intervention by TP resulted in a significant reduction of body weight and improvement in lipid accumulation. And the modulation mechanism by which TP ameliorated the abnormalities of lipid metabolism was associated with the expression levels of lipid metabolism-related genes and serum exosomes miRNA-128-3p, as well as the modulation of structure and abundance of the gut microbiota in mice. In addition, TP treatment significantly increased the content of short-chain fatty acids (SCFAs) in mice feces. The results of molecular docking and dual-luciferase assay exhibited a good interaction between propionic acid and PPAR-α, and it was hypothesized that the interaction might further ameliorate the hyperlipidemia. Therefore, TP can regulate the expression levels of lipid metabolism-related genes through miRNAs from serum exosomes and SCFAs from gut microbiota.
研究领域:
生物化学
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营养学
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分子生物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yongchun Huang,Jie Bai,Jinman Shui,Zhanhai Su,Chengzhu Cao,Haiyan Wang,Qiong Wu,Shoude Zhang
Analysing monosaccharide composition is an essential step in deciphering polysaccharide structures. However, it presents significant challenges due to the pronounced hydrophilicity and the vast array of monosaccharide isomers. Gas/liquid chromatography, coupled with an aldononitrile acetate derivatisation, has been widely used for monosaccharide composition analysis. However, it poses considerable difficulties in simultaneously determining the D/L configurations of these monosaccharides. In this study, a novel method that can simultaneously determine the composition of polysaccharides and their D/L configuration based on L-cysteine methyl ester derivatisation (CMED) and ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) is developed for the comprehensive analysis of monosaccharide types. This method enables the simultaneous characterization of aldoses, ketoses, alditols, and uronic acids. Furthermore, the reaction mechanisms and by-products were meticulously analysed, offering a robust theoretical basis for this novel methodology. Additionally, the developed method was employed to characterize the monosaccharide composition for six plant polysaccharides. Ultimately, the findings demonstrated that the proposed method is effective for confirming monosaccharide composition, encompassing both structural types and absolute configurations.
研究领域:
分析化学
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碳水化合物化学
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生物化学
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植物科学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Sustainable Food Systems
·
2024
·
IF 3.7
Weiwei Xu,Wei Zhou,Jinfeng Sun,Weiwei Chen,Xuanye Wu,Tong Guan,Yilin Zhao,Pengcheng Yang,Zhengyu Hu,Gao Li
The purpose of this study was to obtain polysaccharides from Panax ginseng C. A. Meyer stems (PGSPs), agro-byproducts with development potential, and fully explore the potential value in P. ginseng stems. Two novel polysaccharides firstly from P. ginseng stems (PGSP-1 and PGSP-2) were obtained by water extraction and alcohol precipitation method and column chromatography, and then characterized by FT-IR, HPGPC, HPLC, SEM, TGA, GC–MS and NMR. The results demonstrated that PGSP-1 (Mw = 723.83 kDa) and PGSP-2 (Mw = 620.48 kDa) were characterized →4)-β-D-Galp-(1→, →6)-α-D-Glcp-(1 → and →2)-α-L-Rhap-(1 → as the skeleton, →4,6)-β-D-Galp-(1 → and →2,6)-α-D-Manp-(1 → as the cross junction, α-L-Araf-(1 → as the terminal unit, and PGSP-1 still contained →4)-β-D-Galp, while PGSP-2 contained →4)-β-D-Xylp-(1→, →3)-β-D-GlcpA-(1→, →4)-β-D-GalpA-(1 →) and α-D-Glcp-(1→, with different microstructures and thermal stability. And the results of hypoglycemic activity revealed that both PGSP-1 and PGSP-2 showed excellent inhibitory activity against α-glucosidase and α-amylase, in which PGSP-2 had the better performance. The inhibition kinetics result showed that PGSPs on α-glucosidase and α-amylase were non-competitive type and mixed type inhibition. This study provided a theoretical basis for making full use of and exploiting the economic value of agro-byproducts such as P. ginseng stems and offered a theoretical reference for the effective utilization of PGSP as a functional component to prevent and reduce T2DM.
研究领域:
药理学
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生物化学
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食品科学
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分析化学
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DOI
高影响因子
Journal of CO2 Utilization
·
2024
·
IF 7.2
Yiming Wang,Wensheng Wei,Yuxin Wang,Guangwen Xu,Jinggang Zhao,Lei Shi
In this study, a series of hydroxy-functionalized quaternary ammonium salt catalysts, i.e., NEt 3 (HE)Br, NEt 2 (HE) 2 Br, NEt 1 (HE) 3 Br, and N(HE) 4 Br, were successfully prepared by quantitatively grafting hydrogen-bond donors (HBDs) as electrophilic sites on quaternary ammonium salts and then used to catalyze the cycloaddition of propylene oxide (PO) and carbon dioxide (CO 2 ). The aim was to reveal the effects of different amounts of hydroxy-functionalized quaternary ammonium salts on their catalytic activities. The synergistic catalytic effect of NEt 3 (HE)Br and a biomass-based catalytic system (NEt 3 (HE)Br/Bio) on the cycloaddition reaction was systematically studied, and the influence mechanism of hydroxy-rich biomass on the cycloaddition reaction was elucidated. The promoting effects of the hydroxyl-group content, position and connection structure in different alcohol additives on the CO 2 cycloaddition reaction were further investigated and the results demonstrated that when the additive molecule contains an appropriate number of ortho-hydroxyl groups and these hydroxyl groups are connected to groups with strong electron-withdrawing ability and small steric hindrance, the cycloaddition efficiency between PO and CO₂ can be significantly enhanced. The reason is that these hydroxyl groups not only provide more hydrogen bonds, enhancing the intermolecular interactions, but also help to stabilize the transition state of the reaction. Notably, when 0.48 g of NEt 3 (HE)Br was mixed with 0.48 g of cellulose as HBDs to catalyze the cycloaddition reaction of CO 2 and PO, PO conversion reached 99.34 % within 2 h, exceeding that obtained with the catalytic reaction system without HBDs. In addition, the NEt 3 (HE)Br/cellulose catalytic system was demonstrated to be universal for catalyzing the cycloaddition reaction of different epoxides and CO 2 . This article provides theoretical guidance and new inspirations for the efficient utilization of hydroxy-containing biomass materials in CO 2 conversion.
研究领域:
化学工程
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催化科学
·
环境科学
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产品关联
DOI
高影响因子
Food Bioscience
·
2026
·
IF 6.2
Xiangkun Du,Yasen Wang,Buqing Wang,Yu Shen,Shu Teng,Jiawen Bai,Zijie Li
Saccharomyces cerevisiae spores have emerged as a novel surface display host and have been successfully applied in the biosynthesis of useful products. To construct a dual-enzyme co-display and assembly system on the surface of S. cerevisiae spores, the fluorescent proteins were initially used as the model proteins to prove the feasibility. The result of the in vitro assembly experiment of L-arabinose isomerase from Bacillus coagulans ( Bc AI) and mannose-6-phosphate isomerase from Geobacillus thermodenitrificans ( Gt MPI) confirmed that the assembled complex could significantly improve the biosynthetic efficiency of the rare sugar L-ribose. Subsequently, Bc AI and Gt MPI were co-displayed on the spore surface and further assembled via the SpyTag/SpyCatcher system to realize the biosynthesis of L-ribose. The maximum conversion rate of L-ribose reached approximately 16% with 10 g/L L-arabinose as the substrate. Nevertheless, the achieved L-ribose conversion rate of 16% is moderate compared with previously reported values. Even with this limitation, this study establishes a feasible spore-surface assembly strategy for multi-enzyme cascades, laying a technical foundation for the biosynthesis of high-value products.
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DOI
高影响因子
BIOORGANIC CHEMISTRY
·
2025
·
IF 4.7
Zuoying Wang,Sida Zhang,Yue Li,Zhuoshuo Zhou,Xu Zhang,Ying Chen,Yanli Zhao,Cai Ye,Jinlian Li,Naitian Zhang,Dongmei Wu
The fibrous roots of Atractylodes chinensis are rich in polysaccharides, however, the structure and application of its polysaccharides are still unclear. In this study, a 3.7 kDa inulin-type fructan was purified, which was named ACFP-1. Structural characterization indicated that ACFP-1 was comprised of fructose, arabinose, glucose and galactose. The main chain was →1)- β -Fruf-(2 → 1)- β -Fruf-(2 → linkage type. The branch chain was α -Araf-(1 → 5)- α -Araf-(1 → linkage type, which was connected to the main chain through O-6 of →1,6)- β -Fruf-(2 → glycosidic bond. Activity analysis indicated that ACFP-1 inhibited the generation of ROS in HaCaT cells and AB strain zebrafish. ACFP-1 reduced the positive rate of β-galactosidase and the expression of p53 and p21 protein, restored oxidative stress-induced autophagy inhibition, promoted the expression of LC3II protein, and inhibited the expression of p62 protein in HaCaT cells. These experiments demonstrated antioxidant, anti-aging activity and activation of autophagy of ACFP-1, and ACFP-1 could be used as a potential antioxidant.(补充).
研究领域:
中药学
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分子生物学
·
药理学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Wei Zong,Zijing Liu,Haiyan Lan,Jianwei Yang,Yuxuan Xia,Zhongsheng Xu,Li Mai,Jie Wang,Yixi Bao
The polarization of tumor-associated macrophages (TAMs) from M2 to M1 phenotype is an effective strategy for tumor immunotherapy. In this study, a pectic polysaccharide (PEP-1) was isolated from Phyllanthus emblica fruits via hot water extraction, ethanol precipitation, and purified by anion exchange and gel permeation chromatography. PEP-1 was identified as a homogalacturonan (molecular weight, 156.8 kDa) via characterization analysis. The structural attributes of PEP-1 were elucidated through GC–MS and NMR analyses, confirming that its repeating sugar units consist of GalA connected by α-1,4-glycosidic linkage. Functional assays demonstrated that PEP-1, at concentrations of 50, 100, and 200 μg/mL could strongly induce the transformation of M2 macrophages into M1 phenotype in vitro, and the conditioned medium (CM) of M2 pretreated with PEP-1 significantly promoted apoptosis of Hepa1-6 cells. At the cellular level, PEP-1 shifted tumor-promoting M2 macrophages to a tumor-inhibiting M1 phenotype by increasing the phosphorylation of NF-κB and MAPK. Additionally, PEP-1 (200 mg/kg) was capable of reaching the tumor microenvironment (TME), directly binding to TAMs, promoting their polarization towards M1 phenotype, and significantly inhibiting the tumor growth in Hepa1-6 tumor-bearing mice. These findings reveal the structural characteristics of PEP-1 and its potential to treat hepatocellular carcinoma immunotherapy via regulating TAMs.
研究领域:
免疫学
·
生物化学
·
肿瘤学
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药理学
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碳水化合物化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）