ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Lingyu Nie,Hui Zhang,Weiheng Kong,Rong-Mei Kong,En-Sheng Zhang,Jin Li,Yan Zhao,Fengli Qu
Designing artificial mimetic enzymes with high activity/selectivity to replace chiral bioenzymes is of great interest in the development of chiral materials consisting of molecules, enantiomers, that exist in two forms as mirror images of one another but cannot be superimposed. In this study, the chiral catalytic structural unit was streamlined from tyrosinase to integrate a mimetic nanozyme. The chiral amino acid l-histidine, as the chiral binding/recognition site, and the active metal site Cu were coupled (Cu@l-His) to create a copper–histidine brace with enantioselective catalytic ability to tyrosinol enantiomers. Results of kinetic parameters and activation energies confirmed the excellent peroxidase-like activity with a preference of Cu@l-His to l-tyrosinol. Such a preference could be attributed to the structurally oriented copper–histidine brace with a stronger affinity and catalytic activity to l-tyrosinol. By accurately evaluating chiral recognition units derived from bioenzymes, stable and superior chiral mimetic nanoenzymes could be constructed in a more straightforward and simplified manner, and they could also be extended to the reconstruction of diverse chiral enzymes.
研究领域:
生物化学
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纳米技术
·
催化
·
手性材料
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DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 10
·2167
·
IF 4.2
Yuxia Li,Min Li,Liuzhi Hu,Baozhu Zhang
In this study, Cu2+modulated silver nanoclusters were constructed for the turn-on, label-free detection of L-histidine. Six Ag NCs protected by oligonucleotides (DNA-Ag NCs) were tested in a series of experiments. Finally, A-DAN-Ag NCs were chosen as the best candidate due to their excellent fluorescent properties. The fluorescence of A-DAN-Ag NCs was quenched using Cu2+through energy or electron transfer. However, quenched fluorescence could be restored dramatically in the presence of L-histidine due to Cu2+liberation from A-DAN-Ag NCs and because of the chelation between the imidazole group of L-histidine and Cu2+. The proposed sensor exhibited high selectivity towards L-histidine over other amino acids, with a limit of detection (LOD) of 0.096 μM ranging from 0 to 8 μM. The proposed sensor succeeded in detecting L-histidine in diluted human urine. Therefore, the sensor has promising practical applications in biological systems.
研究领域:
生物化学
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分析化学
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生物医学
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PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Miao Yuan,Saidan Wang,Hongxia Du,Yating Sui,Xiangjing Zhang,Song Zhao,Qiushi Li,Shaojie Liu,Xuteng Xing,Erjun Tang
Two-dimensional Ti 3 C 2 T x MXene, with a large specific surface area, high chemical stability and excellent barrier properties, has become a promising of anti-corrosion filler in organic coatings. However, the poor dispersion and compatibility in organic coatings restrict its efficient application. To this end, L-histidine (L-His) corrosion inhibitor was grafted on the 3-aminopropyl triethoxysilane (APTES) functionalized Ti 3 C 2 T x MXene surface, under the electrostatic attraction between the carboxylate and protonated aminopropyl groups. The obtained APTES-Ti 3 C 2 T x MXene@L-His cannot only accelerate the dispersion of Ti 3 C 2 T x MXene filler, but also greatly promote the anti-corrosion property and service lifetime of coatings through combining the physical shielding effect of MXene laminate structure with active anti-corrosion function of L-His corrosion inhibitor. Results show that amino-functionalized Ti 3 C 2 T x MXene can maintain homogeneous in epoxy resin for 300 h, enhancing its dispersion in epoxy coating. Moreover, APTES-Ti 3 C 2 T x MXene@L-His composite can achieve the continuous release of L-His for 3000 min, and additional acid-base dual response property, the releasing rate of L-His is significantly accelerated in acidic or alkaline solutions. The total loading amount of L-His in APTES-Ti 3 C 2 T x MXene@L-His composite is about 3 %. Furthermore, the practical composite coating containing APTES-Ti 3 C 2 T x MXene@L-His also exhibits the excellent anti-corrosion and certain self-healing properties, such as its |Z 0.01 Hz| value remains above 10 10 Ω·cm 2 during the 40 d immersing in 3.5 NaCl solution, which is 3 orders of magnitude higher than that of pure epoxy coating. This work extends the great significance for Ti 3 C 2 T x MXene based composite coatings application of in the field of anti-corrosion.
研究领域:
材料科学
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腐蚀与防护
·
有机涂层改性
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DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Yuanyuan Li,Qianqian Du,Xiaodan Zhang,Yuming Huang
Tetracycline (TC), a widely-used antibiotic to treat bacterial infections, combines with Eu 3+ to form a stable EuTC complex that has a low fluorescence (FL) yield because of Eu 3+ coordination with water molecules. We report a simple, label-free ratiometric fluorescent platform for sensitively and selectively sensing of TC, using L-histidine caped gold nanoclusters (His-AuNCs) as a FL indicator and an enhancer of Eu 3+ FL. The His-AuNCs were prepared via chemical reduction of Au(III) by L-histidine, which was a reducer and a protecting agent. The His-AuNCs exhibited good photostability, outstanding stability toward high ionic strengths , storage stability, and favorable optical properties . In the absence of TC, the AuNCs-Eu 3+ system displays strong FL emission at 475 nm ( F 475 ) from the His-AuNCs and weak FL at 620 nm from Eu 3+ ( F 620 ) with excitation at 375 nm. TC quenches the His-AuNC FL and greatly enhances the Eu 3+ emission. This is attributed to an enhancement of the EuTC complex fluorescence by the His-AuNCs. Thus, a ratiometric F 620 / F 475 FL signal can be used for TC detection by simply mixing AuNCs and Eu 3+ . Under the optimized conditions, the linear range from 10 nM to 60 μM and a detection limit of 4 nM (S/N = 3) for TC were obtained. The application of the assay platform for the detection of TC in environmental and biological samples was demonstrated. The sensing platform has advantages of easy preparation, rapid response, high sensitivity, and good selectivity.
研究领域:
分析化学
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环境科学
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生物医学
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DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Yongmei Wang,Zongshan Zhao,Jianyuan Qin,Huan Liu,Aifeng Liu,Mengmeng Xu
A simple, green and nondestructive method based on terahertz fingerprint peaks has been developed for rapid in situ analysis of l -histidine and α-lactose in dietary supplements. Fingerprint absorption peaks of l -histidine and α-lactose located at 0.77 and 0.53 THz could be directly used for identification and quantitation of these analytes in commercial dietary supplements. Compared with the partial least squares regression model (PLSR), the linear least squares regression (LLSR) method based on peak areas presented better performance, with the linear correlation coefficients of 0.9899 and 0.9910 for l -histidine and α-lactose, respectively. Furthermore, analysis time per sample can be shortened to less than 1 min due to the narrower spectral acquisition region. The accuracies were 94.8–110% and 98.9–110%, comparable to those of ion chromatography for l -histidine and high-performance liquid chromatography for α-lactose. The results presented great potential of the developed method for rapid in situ analysis of nutrients in dietary supplements.
研究领域:
分析化学
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营养学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Ruichang Gao,Tong Shi,Quancai Sun,Xiuting Li,David Julian McClements,Li Yuan
This research focused on the effects of l -arginine ( l -Arg) and l -histidine ( l -His) on the heat-induced aggregation of fish myosin. l -Arg/ l -His increased the pH of the myosin solution from 6.82 to 8.74 and 7.24, respectively, and decreased the turbidity, aggregate size, shear modulus, and breaking force. The incorporation of l -Arg/ l -His decreased the surface hydrophobicity during setting, but increased it during the two-step heating. The heat-induced aggregation of myosin was suppressed by both amino acids, with the inhibitory effect being greater for l -Arg than l -His. On one hand, the change in the pH played a critical role in suppressing the heat-induced aggregation of myosin. On the other hand, the characteristics of l -Arg/ l -His themselves, such as net charges and particular R-groups, were another main contributor to aggregation suppression. Particularly, l -Arg/ l -His could interact with exposed aromatic residues of myosin, and the interactions may dominate and overwhelm the burial of aromatic residues during two-step heating.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 5
·1-12
·
IF 6.232
Dong Wenjuan,Wang Ruiping,Gong Xiaojuan,Liang Wenting,Fan Li,Song Shengmei,Dong Chuan
A far-red fluorescence “off-on” sensing strategy is described for sequential ratiometric determination of Cu 2+ and L-histidine (L-His) based on fluorescence resonance energy transfer (FRET) system. N,S,P co-doped carbon dots (N,S,P-CDs) and N-acetyl-L-cysteine functionalized gold nanoclusters (NAC-AuNCs) are used in the FRET system, which serve as energy donor and acceptor, respectively. After adding NAC-AuNCs into the solution of N,S,P-CDs, the fluorescence of N,S,P-CDs is effectively quenched, while the far-red fluorescence of NAC-AuNCs appears. Cu 2+ can decrease fluorescence of NAC-AuNCs, and then L-His can effectively recover the fluorescence of NAC-AuNCs. The possible reason is that the stronger affinity between Cu 2+ and L-His can pull Cu 2+ away from the surface of NAC-AuNCs. Through it all, the emission intensity of N,S,P-CDs remains nearly constant, so the ratio of fluorescence intensities at 485 and 625 nm exhibits a linear correlation to the Cu 2+ and L-His concentration, respectively. The sensing platform shows good selectivity towards Cu 2+ and L-His with a linear range of 0.65–26.58 μM and 3.13–56.25 μM and determination limits of 0.50 μM and 0.374 μM, respectively. The proposed method has been successfully used for Cu 2+ and L-His determination in real samples with satisfying results.
Graphical abstract
研究领域:
分析化学
·
生物传感
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2019
·
IF 3.206
Qiqi Tan,Juan Qiao,Rongyue Zhang,Li Qi
Papaya juice protected copper nanoclusters was depicted for detection of serum l -histidine. The fluorescent probe was synthesized by using a simple “one pot” hydrothermal preparation strategy with papaya juice playing dual roles both as a green reductant and stabilizer. The excitation and emission peaks of the copper nanoclusters were at 400 nm and 490 nm, respectively. The fluorescence was turned-on in addition of l -histidine due to the enhanced surface electron density. The fluorescent intensity increased linearly with l -histidine concentration ranging from 2.0 to 50.0 µM. The proposed copper nanoclusters was further applied to determine l -histidine in rat serum after intraperitoneal injection.
研究领域:
生物化学
·
分析化学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Ruihong Yao,Zhijian Li,Panpan Huo,Congcong Gong,Gang Liu,Chunhong Zheng,Shouzhi Pu
In this study, we constructed zinc based metal–organic framework functionalized by L-histidine (His@ZiF-8). The His@ZiF-8 had high porosity, large surface area, abundant carboxyl and amino group, which has been found to greatly enhance the aggregation-induced emission (AIE) of tetracycline (TC). After the His@ZiF-8 enrichment with TC, the TC exhibited strong fluorescence emission peak at 565 nm based on AIE. Under the optimal conditions, the fluorescence intensity of TC exhibited a good linear relationship with TC concentration within 0.1–80 μM with a low limit of detection of 28.6 nM. Antibiotic analogs such as neomycin, chloramphenico, ampicillin, kanamycin, and erythromycin have no obvious interference. In addition, the developed method was successfully used to the detection of TC in milk, river water, and honey.
研究领域:
材料科学
·
分析化学
·
环境科学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2022
·Vol. 57
·期 4
·2556-2567
·
IF 3.612
Xiuyun Guo,Junjun Wu,Xiangren Meng,Yawei Zhang,Zengqi Peng
Summary This study investigated the effects of l -lysine (Lys) and l -histidine (His) on the oxidative characteristics and gel properties of porcine myofibrillar proteins (MP). Results showed that Lys and His had a strong ferrous ion-chelating ability and hydroxyl radical-scavenging activity. Moreover, Lys and His inhibited the protein carbonyl formation and MP aggregation at 0.2 M and 0.6 M NaCl, respectively, in a dose-dependent manner. Furthermore, 2 and 4 mg mL −1 Lys and His decreased the oxidation-induced loss of the tertiary structure of MP accompanied by the lower surface hydrophobicity. The water-holding capacity and gel strength of MP gels increased with increasing Lys and His concentrations due to more regular and lamellar structures with smaller and homogeneous pores at 0.6 M NaCl and more orderly crosslinking via fibrous filament at 0.2 M NaCl. In summary, Lys and His chelated the ferrous ions and scavenged hydroxyl radicals, decreased the oxidation-induced physicochemical changes, thus preventing oxidative damage during the formation of a three-dimensional gel network, which resulted in better gel quality.
研究领域:
食品科学
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生物化学
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DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2014
·
IF 3.05
Cunliu Zhou,Huiqiong Ye,Tadayuki Nishiumi,Hao Qin,Conggui Chen
The objective of this investigation was to evaluate the effects of l -histidine on the stability of porcine hemoglobin concentrates during storage. The results indicated that the addition of l -histidine increased the oxyhemoglobin content and a* values but decreased the methemoglobin content and free iron content (P < 0.05) during storage. The addition of iron cation decreased both the oxyhemoglobin content and a* values but increased both the methemoglobin content and free iron concentration (P < 0.05). CD spectra revealed that the free iron content induced the transformation of the hemoglobin secondary structure from an α-helix (close structure) to a β-pleated, β-turn, and random coil arrangement (loose structure), while l -histidine weakened this behavior of the free iron. Infrared spectra demonstrated that the l -histidine coordinated with the free iron (Fe 2 + or Fe 3 + ) to give the corresponding complex at 25 °C and pH 7.3. Therefore, l -histidine enhanced the stability of hemoglobin concentrates by coordinating with free iron.
研究领域:
生物化学
·
医学工程
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DOI
高影响因子
ANALYST
·
2024
·Vol. 149
·期 6
·1753-1758
·
IF 3.6
Haowei Huang,Junyao Li,Wenrong Cai,Datong Wu,Laidi Xu,Yong Kong
A chiral metal–organic framework (CMOF) was synthesized by introducing L-histidine (L-His) to zeolitic imidazolate framework-8 (ZIF-8) and then grafting with carboxymethyl-β-cyclodextrin (CM-β-CD). Compared with L-His-ZIF-8, the CM-β-CD-functionalized L-His-ZIF-8 (L-His-ZIF-8-CD) showed significantly enhanced discrimination ability for the tryptophan (Trp) enantiomers owing to the inherent chirality of CM-β-CD. The specificity of the chiral interface was also studied, and the results indicated that the discrimination ability for Trp enantiomers is significantly stronger than that for the enantiomers of cysteine (Cys) and tyrosine (Tyr), which might be due to the better matching between the indole ring of Trp and the chiral cavity of CM-β-CD.
研究领域:
化学
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分析化学
·
材料科学
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DOI
PubMed
高影响因子
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