MICROCHEMICAL JOURNAL
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2026
·
IF 5.2
Meiling Long,Yue Liu,Jun Yang,Shaogui Wu
Biomass-derived B-CDs were synthesized from Mahonia fortunei fruits. • The B-CDs showed bright blue emission with a quantum yield of 31.1%. • Pb 2+ selectively quenched B-CD fluorescence with a low LOD of 26 nM. • L-Histidine restored fluorescence via competitive Pb 2+ coordination. • An IMPLICATION logic gate was constructed from the on–off–on response. Nitrogen-doped carbon dots (B-CDs) with deep-blue fluorescence were synthesized through a one-step hydrothermal route using the fruits of the medicinal plant Mahonia fortunei as the biomass carbon source and 1-naphthylamine as the nitrogen dopant. The resulting B-CDs showed excitation-independent emission, a high fluorescence quantum yield of 31.1%, and excellent resistance to salt, photobleaching, and long-term storage. Pb 2+ efficiently quenched B-CD fluorescence by forming non-emissive ground-state complexes through bidentate coordination with oxygen- and nitrogen-containing surface groups, switching the probe to the “off” state. By contrast, L-histidine (L-His), whose imidazole group has stronger competitive affinity for Pb 2+ , dissociated the carbon dot‑lead complexes and markedly restored fluorescence, returning the system to the “on” state. The platform afforded a detection limit of 26 nM for Pb 2+ , while the L-His-induced fluorescence recovery response was linear over 40–80 μM, with an average fluorescence recovery efficiency of 88.2%. On the basis of this reversible fluorescence response, an IMPLICATION molecular logic gate was constructed using Pb 2+ and L-His as chemical inputs and fluorescence intensity as the output, enabling logic discrimination in a two-component system. UV–vis absorption spectroscopy, time-resolved fluorescence lifetime analysis, and FT-IR spectroscopy jointly supported the reversible “on-off-on” response mechanism. This work provides a practical strategy for developing natural plant-derived carbon-dot platforms for reversible fluorescence sensing and molecular logic devices. Download: Download high-res image (155KB) Download: Download full-size image
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Bo Wang,Feng Zhao,Zheng Zhou,Ji Wang,Lei Qin,Xuhui Huang
Studied hazardous compound changes in the thermal processing of 7 animal foods. • Hazards: acrylamide, 5-hydroxymethylfurfural, 13 heterocyclic aromatic amines. • AA, 5-HMF, and 13 HAAs in all animal foods increased with roasting temperature. • Low moisture and high carbs promoted AA; high protein favored HAAs. • Amino acids and dipeptides had negative correlations with hazardous compounds. The thermal processing of food is often accompanied by changes in composition and the generation of hazardous compounds. However, the unclear mechanisms linking food composition to related hazardous compounds hinder the advancement of inhibition technologies for hazardous compounds formed during thermal processing. In this paper, the mechanisms of 15 kinds of associated hazardous compounds produced by 7 different animal-derived food samples during the roasting process were investigated. When the roasting temperature was 260 °C, mutton exhibited the highest acrylamide content at 0.11 μg/g, chicken had the highest HAAs (heterocyclic aromatic amines) content at 0.17 μg/g, and Antarctic krill showed the highest 5-hydroxymethylfurfural content at 0.72 μg/g. notably, the compounds closely related to the production of associated hazards during thermal processing include amino acids, small dipeptides, and certain carbohydrate compounds. The results of this study have positive significance in improving food safety and optimizing production processes. Download: Download high-res image (136KB) Download: Download full-size image
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Catalysis Science & Technology
·
2023
·Vol. 13
·期 13
·3953-3962
·
IF 5
Xuexia Xu,Qin Yang,Lanteng Wang,Jie Zheng,Yang Gu,Xiwen Xing,Jiahai Zhou
The L-proline analogue, L-azetidine-2-carboxylate (L-AZC), is of considerable interest both from biological and medicinal chemistry perspectives. It can be quickly involved in proteins and cause protein misfolding, disrupting the normal function of the protein. L-AZC can be biodegraded as the only carbon and nitrogen source by bacteria, and the hydrolysis on the ring opening of L-AZC has an effective practical detoxification function. We identified an L-AZC hydrolase from Novosphingobium sp. MBES04 (NsA2CH), which belongs to the haloacid dehalogenase-like superfamily and participates in cyclic amino acid metabolism. This enzyme has high substrate and stereospecificity for the hydrolysis of L-AZC. We determined the high-resolution crystal structures of NsA2CH in the form of apo- and covalent complexes with the reaction intermediate. Detailed biochemical, structural, and computational studies provide the molecular mechanism of the substrate and stereoselectivity of NsA2CH. Further bioinformatics analysis revealed 328 NsA2CH homologues (sequence identity more than 60%) widely distributed in bacteria. Our work provides the first structural insight into A2CH covalently complexed with the reaction intermediate. The results will enable the engineering of L-AZC to the building block of aminoglycoside antibiotics.
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Regenerative Biomaterials
·
2023
·
IF 6.7
Chen Qin,Fu Xiaomin,Cai Huawei,Fu Shengxiang,Cai Zhongyuan,Li Mufeng,Wu Xiaoai,Tian Rong,Ai Hua
Precise diagnosis of lymph node metastasis is important for therapeutic regimen planning, prognosis analysis and probably better outcomes for cancer patients. In this work, 68Ga-labeled amphiphilic alternating copolymers nanoparticles with different rigid ligands were synthesized as positron emission tomography (PET) probes for lymph node metastasis imaging. The labeling efficiency and stability of nanoparticles was improved with increased rigidity of coordination unit. PU(68Ga-L-MDI-PEG) nanoparticles (PU(68Ga-L-MDI-PEG) NPs) with the strongest rigidity of coordination unit exhibited the lowest critical micelle concentration, the best 68Ga labeling efficiency and stability. During in vivo lymph node metastasis imaging, PU(68Ga-L-MDI-PEG) NPs led to different accumulations in normal lymph nodes (N-LN) and tumor metastasized sentinel lymph nodes (T-SLN), which resulted in different PET signal presentation, making it feasible to differentiate N-LN from T-SLN. In comparison, small molecule probe 68GaL had poor lymph node accumulation, not only making it difficult to find lymph nodes on PET/computed tomography scan, but also tough to distinguish N-LN from metastatic ones. Overall, this work provides a reference for design of 68Ga labeled polymeric nanoparticles with high chelation efficiency and stability, as sensitive PET probes for lymph node imaging.
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PHOTOCHEMISTRY AND PHOTOBIOLOGY
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2022
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IF 3.521
Yu Zhang,Dekun Cheng,Zhangtie Wang,Yiwen Guo,Ming Chang,Ruijie Liu
Excessive ultraviolet (UV) exposure is the primary environmental factor that contributes to skin aging. Orbitides have been considered as important functional components in linseed, and they are complex and multiple. In this study, linseed orbitides (LOs) were divided into oxidized linseed orbitides (OLOs) and reduced linseed orbitides (RLOs) and used to investigate protection against ultraviolet B-induced photoaging in zebrafish. First, the results of the zebrafish embryo acute toxicity test showed that the OLOs had obvious embryo toxicity compared with RLOs. And RLOs had better effect in ultraviolet B-treated zebrafish, which may significantly reduce the reactive oxygen species levels and inhibit the degradation of collagen. Besides, we also found that RLOs could effectively inhibit the oxidation of proteins and lipids and regulate the activity of antioxidant enzymes. Furthermore, neutrophil recruitment to the dorsal and caudal fin regions was observed in UVB-treated zebrafish, and RLOs effectively alleviated this migration. In conclusion, RLOs have strong photoprotective effects and potential to be used as antiphotoaging ingredients.
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Journal of Materials Chemistry B
·
2022
·Vol. 11
·期 2
·359-376
·
IF 7.571
Mingyue Liu,Xiangsheng Wang,Jie Cui,Hongsheng Wang,Binbin Sun,Jufang Zhang,Bernd Rolauffs,Muhammad Shafiq,Xiumei Mo,Zhanyong Zhu,Jinglei Wu
Antibacterial, anti-inflammatory, and pro-angiogenic properties are prerequisites for dressing materials that accelerate the healing process of infected wounds. Herein, we report a magnesium-doped silica bioactive glass (SiO2/MgO) nanofiber membrane prepared by electrospinning. Our results demonstrate that this SiO2/MgO nanofiber membrane has good flexibility and hydrophilicity, which give it intimate contact with wound beds. In vitro assessments illustrate its good cytocompatibility and bioactivity that contribute to its robust cell proliferation and angiogenesis. It shows capacity in modulating the cellular inflammatory response of murine macrophages. In addition, in vitro assays prove its good antibacterial activity against both Gram-positive and Gram-negative strains. In a full-thickness skin defect inoculated with Staphylococcus aureus in mice, it effectively inhibits bacterial infection. Both gene expression and histological/immunohistochemical analyses confirmed the down-regulated pro-inflammatory factors, up-regulated anti-inflammatory factors, and enhanced angiogenesis. Taken together, these desirable properties work in concert to contribute to the rapid healing of infected wounds and make it a good candidate for wound dressing materials.
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JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
·
2022
·Vol. 111
·期 1
·132-151
·
IF 4.854
Mingyue Liu,Weixing Zhang,Zhe Chen,Yangfan Ding,Binbin Sun,Hongsheng Wang,Xiumei Mo,Jinglei Wu
Previously, we demonstrated that magnesium oxide (MgO)-incorporated electrospun membranes show powerful antibacterial activity and promote wound healing, but the underlying mechanisms have not been entirely understood. Herein, we investigated the relationship between structure and function of MgO-incorporated membranes and interrogated critical bioactive cues that contribute to accelerated wound healing and functional restoration. Our results show that MgO-incorporated membranes exhibit good flexibility and improved water vapor transmission rates (WVTRs) and sustained Mg 2+ release in a simulated model of wounds. MgO-incorporated membranes modulate macrophage phenotype to downregulate inflammatory response, contributing to alleviated inflammation and creating a favorable microenvironment for wound healing. Specifically, MgO-incorporated membranes stimulate macrophages to shift to a pro-healing M2 phenotype and upregulate pro-healing cytokine of transforming growth factor-beta 1 (TGF-β1) and downregulate pro-inflammatory cytokines under lipopolysaccharide (LPS) challenge conditions. Together with increased TGF-β1 by macrophages, MgO-incorporated membranes significantly boost the proliferation of fibroblasts and upregulate collagen production, thus driving granulation tissue formation and wound closure. MgO-incorporated membranes promote angiogenesis by promoting tube formation and upregulating vascular endothelial growth factor (VEGF) production of endothelial cells. Rapid epithelialization of regenerated skin tissue is attributed to the balanced phenotype of keratinocytes between proliferative and terminally differentiated populations. In addition to coordinating keratinocyte phenotype, MgO-incorporated membranes reduce the expression of inflammatory cytokine interleukin 1-alpha (IL-1α) therefore promoting hair follicle regeneration. These data provide mechanisms of MgO-incorporated membranes that inhibit bacterial infection, alleviate inflammation, facilitate extracellular matrix production and epithelialization, and potentiate hair follicle regeneration.
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JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
·
2022
·Vol. 110
·期 11
·1824-1839
·
IF 4.854
Yangfan Ding,Weixing Zhang,Binbin Sun,Xiumei Mo,Jinglei Wu
Decellularized meniscus extracellular matrix (dmECM)-based biological scaffolds in the forms of sponge, hydrogel, nanofiber, and composite have gained increasing interest in meniscus tissue engineering and regeneration. A common shortcoming of those scaffolds is insufficient mechanical strength and poor elasticity. Herein, we report a practicable protocol for milder meniscus decellularization to prepare elastic, porous dmECM scaffolds. Porcine meniscus was pulverized by cyclic freeze–thaw grinding and then treated with DNase to obtain fine dmECM particles. Individual dmECM particles were condensed to bulk preparation by centrifuge, followed by lyophilization to form blocks, and finally crosslinked by dehydrothermal treatment to obtain porous dmECM scaffolds. Our results show that the freeze–thaw grinding method was effective in removing cellular DNA with good retention of meniscus-derived bioactive components. The dmECM scaffold had porous structure with interconnected mesopores and good mechanical properties. Primary articular chondrocytes proliferated robustly and maintained chondrogenic characteristics and produce abundant collagen on dmECM scaffolds. Evaluation of biocompatibility in a rat model shows that the dmECM scaffold elicited minor foreign body reactions, indicating effective antigen removal from dmECM. This study provides an alternative for preparing dmECM and fabricating porous scaffolds for meniscus repair and regeneration.
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PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
·
2021
·
IF 6.158
Yan Bao,Lu Gao,Feitong Wang,Jianzhong Ma
Gemini surfactants are highly demanded worldwide due to their promising applications in paper, medical hygiene, and textile. However, the design of Gemini surfactant integrated with biosafety and multi-functionality has been considered a long-standing demand for environmental protection and industry cost. Hence, three kinds of heterocyclic cationic Gemini surfactants (C 12 -2 P-C 12 , P C 4 H 5 N, C 4 H 9 NO, C 3 H 4 N 2 ) with different hydrophilic groups were synthesized and employed to enhance antibacterial, dispersion, and fixation in leather processing. Their surface activity was measured by a series of analytical and testing methods, including the surface tension (γ cmc ), wettability and emulsifying ability. The results showed that the surface activity of the Gemini surfactants were followed the order of C 12 -2 C 4 H 5 N-C 12 (γ cmc =28.65 mN/m)< C 12 -2 C 4 H 9 NO-C 12 (γ cmc =25.85 mN/m)< C 12 -2 C 3 H 4 N 2 -C 12 (γ cmc =23.31 mN/m). Their antibacterial activities were assessed by means of Staphylococcus aureus . C 12 -2 C 3 H 4 N 2 -C 12 obtained a superior antibacterial rate (~100%) owing to its high charge density. And compared with the traditional soaking programs (JFC and commercial fungicides), the sheepskins treated by C 12 -2 P-C 12 exhibited favorable antibacterial properties and fiber dispersion . Among them, the protein content in the soaking solution containing C 12 -2 C 3 H 4 N 2 -C 12 increased notably by 68.0 μg/mL in contrast to the traditional soaking solution (23.8 μg/mL), indicating that C 12 -2 C 3 H 4 N 2 -C 12 has the best effect for fiber dispersion. For dyeing processing, the color fastness of the sheepskin treated by C 12 -2 C 3 H 4 N 2 -C 12 could be improved from level 4 to level 4–5 compared to the commercial dye-fixing agent. And it led to significant changes of the dye concentration in waste liquid (from 1.1 g/L for comprasion to 0.1 g/L for C 12 -2 C 3 H 4 N 2 -C 12 ), which may be imposed on the possibilities of the sustainable development of leather processing.
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APPLIED CATALYSIS A-GENERAL
·
2023
·
IF 5.5
Hongxian Luo,Gang Li,Longxin Ju,Qiang Lv
Herein, an environmentally friendly and compositionally tunable proline-functional polymer was prepared by copolymerization of divinylbenzene (DVB) with L-proline functional monomer . The synthesized poly[DVB-(4-APCA) x ]s were characterized by Powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FT-IR), solid state 13 C cross-polarization/magic angle spinning NMR (solid state 13 C CP/MAS NMR), thermogravimetry-Mass (TG-MS). The combined characterization results elucidated the skeletal structure of the polymer. The proline-functional polymer was used as catalyst in the preparation of methacrolein (MAL) by liquid phase condensation of formaldehyde (FA) and propionaldehyde (PA), in which the conversion of PA is 73.3%, the selectivity of MAL is 85.6% and the yield of MAL is 62.8% under 0.1 g poly[DVB-(APCA)]s at 75 °C for 4 h when dimethyl sulfoxide (DMSO) is used as the solvent. Its catalytic performance did not degrade markedly after five cycles. Moreover, the kinetics of the condensation reaction was also studied using the isolation method.
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Applied Sciences-Basel
·
2021
·Vol. 11
·期 10
·4578
·
IF 2.679
Xiaofeng He,Liling Xie,Xiaoshan Zhang,Fan Lin,Xiaobo Wen,Bo Teng
Aged swim bladders from the yellow drum (Protonibea diacanthus) are considered collagen-based functional food with extremely high market value. The structural integrity of collagen may be crucial for its biological functions. In the current study, swim bladders with 25-year-old sequences were collected and found to be basically composed of collagen. Then, thermogravimetry (TG), differential scanning calorimetry (DSC), X-ray diffraction (XRD), and attenuated total reflectance–Fourier transform infrared spectroscopy (ATR–FTIR) were conducted to evaluate the integrity of the peptide chain and triple helix in the collagen. The structures of microfibers and fiber bundles were revealed with atomic force microscopy (AFM), scanning electrical microscopy (SEM), and optical spectroscopy. The collagens in the aged swim bladders were found to have similar thermal properties to those of fresh ones, but the relative content of the triple helixes was found to be negatively correlated with aging. The secondary structure of the remaining triple helix showed highly retained characteristics as in fresh swim bladders, and the microfibrils also showed a similar D-period to that of the fresh one. However, the fiber bundles displayed more compact and thick characteristics after years of storage. These results indicate that despite 25 years of aging, the collagen in the swim bladders was still partially retained with structures.Keywords:collagen;triple helix structure;swim bladder;aging
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2020
·
IF 3.209
Mingjie Deng,Feiyan Lin,Caiping Zhou,Yuyan Chen,Leijie Xuan,Hongzhe Wang,Tiantian Feng,Lufeng Hu
Amino acids (AAs) are abundant in seminal fluid and play an important role in the reproduction process. However, the functional significance of AAs in seminal is not clear. This study was designed to profile the AAs in seminal fluid of asthenozoospermia (AS) and oligospermia (OL) patients and evaluate its potential diagnostic value. To determine the true function of AAs in seminal fluid, an ultra high performance liquid chromatography-mass spectrometry (UPLC-MS/MS) was developed and validated. The seminal fluid samples collected from 76 AS patients, 58 OL patients, and 76 healthy subjects were determined by UPLC-MS/MS and analyzed by fisher discriminant and receiver operating characteristic (ROC). The results showed that, the AAs levels were dramatically decreased in AS and OL patients compared with healthy subjects. What’s more there were significant variation in the AAs profile of AS and OL patients. Specifically, some polar AAs decreased, while nonpolar AAs increased in OL patients. Based on the level of AAs, 93.4 % of original and 90.9 % of cross-validated grouped cases were correctly classified between AS patients, OL patients, and healthy subjects. The further ROC analysis shows that glutamic acid, tyrosine, aspartic acid, proline, and tryptophan have the diagnostic value (P < 0.01) in the three groups. Moreover, aspartic acid and glutamic acid exhibit a high correlation (Correlation Coefficient >0.9) in AS, OL, and healthy subjects. In conclusion, there are significant variation in AAs profile in seminal plasma of AS and OL patients with high accuracy in fisher classification. The associations between the levels of AAs in seminal fluid of AS, OL, and healthy subjects may have clinical applications in providing valuable diagnostic indicator.
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