APPLIED SURFACE SCIENCE
·
2015
·
IF 2.711
Jiasong Zhong,Qingyao Wang,Daqin Chen,Leifeng Chen,Hua Yu,Hongwei Lu,Zhenguo Ji
In this work, we developed a novel environmentally friendly strategy toward solvothermal synthesis of 3D hierarchical Cu 2 FeSnS 4 microspheres with the utilization of l -cystine, an amino acid, as a coordination agent and sulfur donor. The crystal structure, surface morphology and microstructure of the as-prepared products were investigated. The results showed that the 3D hierarchical Cu 2 FeSnS 4 microspheres were composed of stannite nanosheets, and the average thickness of nanosheets was 100 nm. Moreover, the 3D hierarchical Cu 2 FeSnS 4 exhibited high efficiency in the photodegradation of RhB under visible light illumination, suggesting a promising candidate for treatment of organic pollutants in waste water.
研究领域:
材料科学
·
环境科学
·
化学
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产品关联
DOI
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2023
·Vol. 34
·期 5
·1608-1621
·
IF 3.4
Xujian Li,Xumin Chen,Zhixiao Ji,Luqi Pan,Yi Liu,Xiao Yang,Changcan Shi
Rapid hemostasis, biosafety, and adaptability to different bleeding sites are the focus of research on hemostatic materials. In this work, an efficient and simple preparation of aldehyde starch hemostatic microspheres (ASHMs) crosslinked with L-cystine dimethyl ester was developed. Through a series of physicochemical parameters and in vitro and in vivo hemostatic properties measurements, the results showed that under the optimal crosslinking degree, ASHMs had a regular and uniform spherical shape, and it had a liquid suction capacity of up to 880%. At the same time, the aldehyde groups on its surface can increase the adhesion and aggregation of blood cells, thereby enhancing the hemostatic effect, which can be considered as a synergistic effect. More importantly, ASHMs have good biocompatibility and minimal intradermal irritation. Taken together，ASHMs could be used as potential novel hemostatic materials for surgical treatment.
研究领域:
生物材料学
·
医学工程
·
外科治疗
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产品关联
DOI
高影响因子
Polymers
·
2016
·Vol. 8
·期 2
·36
·
IF 2.944
Liang He,Di Li,Zhongtang Wang,Weiguo Xu,Jixue Wang,Hui Guo,Chunxi Wang,Jianxun Ding
Smart polymer nanogel-assisted drug delivery systems have attracted more and more attention in cancer chemotherapy because of their well-defined morphologies and pleiotropic functions in recent years. In this work, anl-cystine-crosslinked reduction-responsive polypeptide nanogel of methoxy poly(ethylene glycol)-poly(l-phenylalanine-co-l-cystine) (mPEG-P(LP-co-LC)) was employed as a smart excipient for RM-1 prostate cancer (PCa) chemotherapy. Doxorubicin (DOX), as a regular chemotherapy drug, was embedded in the nanogel. The loading nanogel marked as NG/DOX was shown to exhibit glutathione (GSH)-induced swelling and GSH-accelerated DOX release. Subsequently, NG/DOX showed efficient cellular uptake and proliferation inhibition. Furthermore, NG/DOX presented enhanced antitumor efficacy and security in an RM-1 PCa-grafted mouse modelin vivo, indicating its great potential for clinical treatment.Keywords:chemotherapy;l-cystine;polypeptide nanogel;prostate cancer;reduction-responsiveness;smart drug delivery
研究领域:
药学
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
CORROSION SCIENCE
·
2024
·
IF 7.4
Xin Guo,Xiaolun Ding,Yaxin Wang,Jinke Wang,Weimin Tan,Yiran Li,Zhibin Chen,Zongbao Li,Weiting Chen,Lingwei Ma,Dawei Zhang
A high-throughput screening method is developed for rapid evaluation of corrosion inhibitors. By employing a fluorometric technique, this method quickly assesses the concentration of iron ions converted from corrosion products in the reaction pool to rapidly evaluate the corrosion inhibition performance of amino acid and surfactants. Utilizing this high-throughput screening platform, the corrosion inhibition effects of L-cysteine mixed with quaternary ammonium salts of different alkyl chain length were evaluated, and their optimal mixing ratios were determined. Through corrosion experiments and molecular simulations, the synergistic mechanisms of the inhibitor mixtures are revealed.
研究领域:
材料科学
·
化学工程
·
腐蚀科学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 7
·1-10
·
IF 5.7
Zhang Yujie,Yu Jiarui,Huang Wanjin,Jin Zhenhuan,Li Jianping
A novel extended-gate field-effect transistor (FET) photoelectrochemical (EGFET PEC) sensor was designed for highly sensitive detection of L-cysteine (L-Cys). TiO 2 was initially modified on the ITO electrode by the sol–gel dip-coating method and calcined to produce TiO 2 /ITO. Then, CdS was synthesized on the TiO 2 surface by hydrothermal method to obtain the CdS–TiO 2 heterojunction material. CdS/TiO 2 /ITO was connected to the gate of the FET to obtain an EGFET PEC sensor. Under the irradiation of a xenon lamp simulating visible light, the CdS/TiO 2 heterojunction composite absorbs light energy to produce photogenerated electron–hole pairs, which have strong photocatalytic oxidation activity and oxidize L-Cys covalently identified by Cd(II) through CdS covalent. These pairs generate a photovoltage that controls the current between the source and the drain to detect L-Cys. Under the optimized experimental conditions, the optical drain current ( I D ) of the sensor exhibited a good linear relationship with the logarithm of L-Cys in the range of 5.0 × 10 −9 –1.0 × 10 −6 mol/L, and the detection limit was 1.3 × 10 −9 mol/L (S/N = 3), which is lower than the values reported by other detection methods. Results showed that the CdS/TiO 2 /ITO EGFET PEC sensor revealed high sensitivity and good selectivity. The sensor has been used to determine L-Cys in urine samples. Graphical abstract
研究领域:
分析化学
·
生物传感器
·
材料科学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2024
·
IF 14.3
Xiaoxiao Ma,Lizhi Lin,Hang Luo,Qianqian Zheng,Hui Wang,Xiaoyan Li,Zhenfei Wang,Yongqiang Feng,Yu Chen
In recent years, negative pressure wound dressings have garnered widespread attentions. However, it is challenging to drain the accumulated fluid under negative pressures for hydrogel dressings. To address this issue, this study prepared a chemical/physical duel-network PEG-CMCS/AG/MXene hydrogel composed by chemical disulfide crosslinked network of four-arm polyethylene glycol/carboxymethyl chitosan (4-Arm-PEG-SH/CMCS), and the physical network of hydrogen bond of agar (AG). Under near-infrared light (NIR) irradiation, the PEG-CMCS/AG/MXene hydrogel undergoes photothermal heating due to integrate of MXene, which destructs the hydrogen bond network and allows the removal of exudate through a mechanism mimicking the sweat gland-like effect of skin pores. The photothermal heating effect also enables the antimicrobial activity to prevent wound infections. The excellent electrical conductivity of PEG-CMCS/AG/MXene can promote cell proliferation under the external electrical stimulation (ES) in vitro. The animal experiments of full-thickness skin defect model further demonstrate its ability to accelerate wound healing. The conversion between thioester and thiol achieved with L-cysteine methyl ester hydrochloride (L-CME) can provides the on-demand dissolution of the dressing in situ. This study holds promises to provide a novel solution to the issue of fluid accumulations under hydrogel dressings and offers new approaches to alleviating or avoiding the significant secondary injuries caused by frequent dressing changes.
研究领域:
材料科学
·
生物医学工程
·
伤口护理
·
组织工程
·
纳米技术
·
光热治疗
·
药物递送系统
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Jia Jiang,Shuang Zang,Dan Li,Kun Wang,Sizhu Tian,Aimin Yu,Ziwei Zhang
Electron spin resonance spectroscopy was applied to determining the antioxidant capacity of eight thiol-containing compounds, including reduced glutathione , N-acetyl-L-cycsteine, methimazole, captopril , and tiopronin with one thiol group , 1,4-dithioerythritol and 2,3-dimercapto-1-propanol with two thiol groups, as well as L-cystine with no free thiol group. Cu 2+ ion gives an electron spin resonance signal and is reduced to Cu + ion with no electron spin resonance signal by the free thiol group in the compounds. Trolox equivalent antioxidant capacity (TEAC) was used to evaluate the reducing ability of the thiol-containing compounds and the TEAC values were found to be relevant to the number of thiol groups contained in the compounds. For the purpose of comparison, the UV-vis spectrophotometry , cupric reducing antioxidant capacity (CUPRAC) method, and Ellman assay were applied to the determination of the antioxidant capacity of the thiol-containing compounds. The TEAC values obtained by the present method were very close to those obtained by UV-vis method. However, compared with CUPRAC method, for methimazole the present method gave a more reasonable TEAC value. The present method was also applied to the quantification of N-acetyl-L-cycsteine, methimazole, captopril, and tiopronin in their pharmaceutical formulations.
研究领域:
分析化学
·
药物分析
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2025
·
IF 4.8
Shasha Zhou,Xinyu Cao,Xuefei Yin,Jiawen Xu,Kai Gao,Zhenshou Wang
Cysteine-engineered antibodies (THIOMABs) are pivotal in the site-specific conjugation for ADC therapeutics. In this study, THIOMABs were expressed in CHO cell culture and showed a significantly higher proportion of acidic variants compared to traditional antibodies. A dual formation mechanism for acidic species was identified: glutathione (GSH) capping at engineered cysteine sites and traditional post-translational modifications (PTMs) during cell culture. Moreover, it was found that these two mechanisms exhibited overlapping effects on the formation of acidic species, and their simultaneous elimination was required for significant reduction of acidic variants. Consequently, by modulating temperature and pH to reduce PTMs and supplementing with L-cysteine to displace GSH, the proportion of acidic variants was successfully reduced. This study uncovers dual formation mechanisms of acidic species in THIOMABs and provides a practical cell culture approach, effectively reducing acidic variants and enhancing the quality attributes of THIOMABs.
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2025
·
IF 4.3
Ming Li,Xiaofei Fan,Jianfeng Li,Jianwei Mao,Yuchen Zhang,Rujie Wang,Huining Xiao
In this study, a novel alginate-based composite hydrogel sphere embedded with zeolite imidazole framework (ZIF-8) and carbon dots (CDs) was developed for simultaneous efficient adsorption and selective detection of mercury ions (Hg 2+ ). The hydrogel spheres (Z8S-CDs) integrate the porous structure of ZIF-8 for efficient Hg 2+ adsorption and the fluorescence properties of CDs for visual detection. The adsorption capacity of Z8S-CDs reached 381.67 mg/g for Hg 2+ , significantly surpassing conventional alginate hydrogels, attributed to synergistic chemisorption and pore-filling mechanisms. Meanwhile, the hydrogel spheres exhibited selective fluorescence quenching upon Hg 2+ binding, enabling rapid detection with a low detection limit (3.63 × 10 −8 M) and high specificity against competing ions. The hydrogel spheres maintained structural stability and reusability over multiple cycles. This work provides a dual-functional platform for effective Hg 2+ removal and real-time monitoring in contaminated water, highlighting its potential for environmental remediation applications.
研究领域:
环境科学
·
材料化学
·
分析化学
·
水体修复
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2026
·Vol. 98
·期 33
·24278-24289
·
IF 7.3
Dai Jiaxing,Shi Jiahui,Zhang Tao,Chang Lei,Zhang Yao,Xie Yuping,Fu Bin,Lyu Zhitang,Xu Ping
LysN is a valuable protease in proteomics
because it cleaves peptide
bonds N-terminal to lysine, generating peptides with physicochemical
properties complementary to those produced by LysC and trypsin. However,
the broader adoption of LysN in proteomic workflows has been limited
by the lack of commercially available enzymes that combine high activity,
low missed-cleavage rates, and sufficient stability under practical
sample-processing conditions. Here, we report the recombinant production
and proteomic characterization of a self-digestion-resistant and highly
active LysN from Shewanella loihica (SL-LysN). Using terminomics, we mapped the mature N- and C-termini
of the enzyme and established the primary structure of the active
protease. We further developed a high-density fermentation, refolding,
and purification workflow to obtain highly purified recombinant SL-LysN.
Biochemical and proteomic benchmarking showed that SL-LysN displayed
3.3-fold higher specific activity than commercial LysN and reduced
missed cleavages by approximately 80%. Notably, SL-LysN retained high
activity in the presence of 8 M urea or 1% SDS and showed strong resistance
to autolysis, indicating exceptional robustness for proteomic sample
preparation. In complex mammalian proteome digests, SL-LysN achieved
>95% cleavage specificity and a missed-cleavage rate of only 5.9%.
These features address a long-standing bottleneck in N-terminal proteolysis
and establish SL-LysN as a high-performance enzymatic tool for advanced
proteomic workflows, including deep protein sequencing, quantitative
proteomics, terminomics, de novo sequencing and analyses
requiring efficient digestion under denaturing conditions.
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产品关联
DOI
高影响因子
VIBRATIONAL SPECTROSCOPY
·
2025
·
IF 3.1
Yusong Zhang,Wei Shi,Yifan Li,Huanlin Li,Junnan Wang
Changes in antibiotic and amino acid concentrations are closely related to human beings, the natural environment and animal and plant life and health. Real-time and label-free detection using terahertz time-domain spectroscopy (THz-TDS) is of great significance for disease prevention and treatment and drug residue detection. However, water has a strong absorption of THz waves that cannot be ignored, which hinders the detection of water-containing samples. In this paper, the characteristic absorption peaks and molecular vibration modes of penicillin sodium and L-hydroxyproline were studied based on THz-TDS and density functional theory (DFT) using IPCA with high radiation properties. The absorption peaks of samples in different states are different, and the formation of new hydrogen bonds will cause the absorption peak to disappear, or a blue shift or a red shift occurs. The intramolecular interaction forces were then characterized based on IRI analysis. Based on this method, solutions with a minimum concentration of 842 μM can be qualitatively tested.
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产品关联
DOI
高影响因子
ANALYST
·
2025
·
IF 3.3
Xianwen Kan,Haotian Xue
The development of integrated dual signal outputs for reliable and accurate determination of glutathione (GSH) is highly significant for its key role in the physiological processes. Herein, a colorimetric/photoelectrochemical (PEC) dual-mode sensing platform was constructed based on the GSH triggered decomposition of a copper-organic framework (CuHPT). A hollow nanostructured type-II heterojunction of CoS/In-CdS was synthesized using imidazolate framework (ZIF-67) as a template. CuHPT was decomposed in the presence of GSH to form catechol ligands and Cu+, enabling the dual-mode sensing. An ion exchange reaction between the produced Cu+ and Cd2+ of CoS/In-CdS resulted in the formation of Cu2S, causing the decrease of the photocurrent and the sensitive detection of GSH. Cu+ catalyzed H2O2 to produce ·OH via a Fenton-like reaction, achieving the colorimetric sensing. The proposed dual-mode sensor based on the target-triggered decomposition of CuHPT exhibited linear responses for colorimetric and PEC sensing of GSH in ranges of 0.05 - 1.20 mM and 0.5 - 800 μM, with the limit of detections (LODs) of 18 μM and 0.11 μM, respectively. The reliable determination of GSH in human serum provides a new possibility for application of the present dual-mode sensor in clinical assay.
研究领域:
生物传感
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）