Antibiotics-Basel
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2022
·Vol. 11
·期 12
·1737
·
IF 5.222
Fang Zhou,Elie Kostantin,De-Quan Yang,Edward Sacher
Although Ag nanoparticles (NPs) have been widely applied in daily life and in biomedical and industrial fields, there is a demand for Ag-based bimetallic nanoalloys (NAs), such as AgCu and AgFe, due to their enhanced antibacterial efficacy and reduced Ag consumption. In this work, we present a comparison study on the antibacterial efficacy and cytotoxicity rates of Ag NPs and AgCu and AgFe NAs to L929 mouse fibroblast cells using the CCK-8 technique based on the relative cell viability. The concept of the minimum death concentration (MDC) is introduced to estimate the cytotoxicity to the cells. It is found that the minimum inhibitory concentrations (MICs) of the NPs againstE. coliandS. aureusdecrease with the addition of both Cu and Fe. There is a strong correlation between the MDC and MIC, implying that the mechanisms of both antibacterial efficacy and cytotoxicity are similar. The enhanced antibacterial efficacy to bacteria and cytotoxicity toward the cell are attributed to Ag+release. The following order is found for both the MIC and MDC: AgFe < AgCu < Ag NPs. However, there is no cytotoxicity to the L929 cells for AgFe and AgCu NAs at their MIC Ag concentrations againstS. aureus.Keywords:AgCu;AgFe;antibacterial efficacy;cytotoxicity;L929 cells;nanoalloy
研究领域:
材料科学
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生物学
·
医学
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产品关联
DOI
PubMed
高影响因子
Canadian Respiratory Journal
·
2019
·
IF 1.803
Sheng Zuo,Zhen Zhu,Yi Liu,Hong Li,Shuang Song,Shaojun Yin
Aim. The transmembrane chemokine (C-X-C motif) ligand 16 (CXCL16) plays a vital role in the pathogenesis of organ fibrosis, including the liver and kidney. However, the detailed biological function of CXCL16 is still not fully understood in the progression of pulmonary fibrosis (PF). The aim of present study is to examine the function of CXCL16 in PF. Materials and Methods. In this study, we constructed the PF model on mouse by using bleomycin and analyzed the effect of the mouse recombinant protein CXCL16 on mouse lung fibroblast L929 (LF) as well. To further examine the connection between CXCL16 and STAT3 in mouse LF cells, the STAT3 inhibitor AG490 was utilized to inhibit the expression of STAT3. Meanwhile, lipopolysaccharide was used to enhance the phosphorylation of STAT3 (p-STAT3) in mouse LF cells. Results. Our results indicated that the level of CXCL16/CXCR6 was significantly upregulated in the mouse PF model. Moreover, the level of p-STAT3 was also promoted. In addition, the mouse recombinant protein CXCL16 not only contributed to the proliferation of mouse LF cells but also induced the expression of p-STAT3 in LF cells. However, the effect of CXCL16 was deeply abolished by the STAT3 inhibitor AG490 in LF cells. Meanwhile, the antibody of CXCL16 deeply reduced the phosphorylation of STAT3 in lipopolysaccharide (LPS) cultured cells. Conclusions. All these results demonstrated that CXCL16 promoted the phosphorylation of STAT3 and further demonstrated that STAT3 was a critical component in CXCL16/CXCR6 signaling pathway. This research not only enhanced the comprehension of CXCL16 but also indicated its potential value as a target in the treatment for human PF.
研究领域:
肺科学
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分子生物学
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免疫学
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DOI
PubMed
高影响因子
DYES AND PIGMENTS
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2020
·
IF 4.613
Jing-Yang Ni,Xiao-Feng Weng,Ru Sun,Yu-Jie Xu,Jian-Feng Ge
1,8-Naphthalimide is widely applied in the construction of probes because it is easy to be modified. However, 1,8-naphthalimide has been reported rarely in the application of organelle labelling especially lipid droplets (LDs). Herein, three types of amine compounds, aliphatic amine ( a , b ), aromatic amine ( c , d ) and heterocyclic amine ( e , f ), were chosen to construct fluorescent dyes ( 1a - 1f ) with 4-bromo- N -butylnaphthalimide ( 1 ) by Ullmann reaction. The maximum emission wavelength ranged from 490 nm to 550 nm. What's more, 1a-1e showed high fluorescence quantum yields (0.54–0.93) in chloroform and 1e-1f had excellent photostability (>92%). In addition, the cell imaging experiment showed that they could label lipid droplets for HeLa cancer cells and L929 normal cells with extremely low toxicity. This work realizes the classical fluorophore 1,8-naphthalimide to synthesize a series of LDs markers without complex synthesis steps.
研究领域:
化学
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生物学
·
材料科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
·
IF 5.162
Ting Su,Wei Zhao,Lipeng Wu,Wei Dong,Xiaoliang Qi
With the rapid development of biomedicine applications, more flexible and efficient fabrication strategies are required for drug delivery vehicles. Naturally resourced polymers have gained considerable attention for construction of drug carriers. Here, biocompatible hydrogel reservoir system (named PHG-PDA) based on pullulan and polydopamine (PDA) are designed and fabricated by one-pot incorporation of PDA fibers into pullulan hydrogel (PHG). The structure and performance of the PHG-PDAs can be nicely adjusted by regulating the content of PDA fibers in precursor hydrogel solutions. Crystal violet is used as a drug prototype and introduced into hydrogels via swelling-diffusion approach. We found that the drug loading process followed the pseudo-second-order model and Langmuir isotherm model, while the drug releasing process was pH-responsive, and the cumulative release reached 60.3% and 87% at pH 7.4 and 5.0, respectively. In vitro cytotoxicity studies on L929 cells demonstrated that our hydrogels were nontoxic even in a high concentration (3.4 mg/mL). In sum, these biocompatible PHG-PDAs with tunable physicochemical properties are promising systems for drug delivery.
研究领域:
生物医学工程
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材料科学
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药剂学
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产品关联
DOI
PubMed
高影响因子
POLYMER INTERNATIONAL
·
2022
·Vol. 71
·期 11
·1278-1286
·
IF 3.213
Miao Dai,Siyuan Li,Yan Cui,Wei Zhang,Hui Shi,Kai Pan,Wei Wei,Xiaoya Liu,Xiaojie Li
Although magnesium (Mg) and its alloys are promising biodegradable materials for orthopedic implants, rapid and uncontrollable degradation of Mg is still a challenge for clinical applications. In this work, electroactive polymer coatings were prepared to improve the corrosion resistance and cytocompatibility of Mg alloys. Amphiphilic electroactive tetraaniline (TANi)-conjugated poly( γ -glutamic acid) ( γ -PGA) with various TANi modification rates ( γ -PGA–TANi 10 , γ -PGA–TANi 20 , γ -PGA–TANi 27 ) were first synthesized. γ -PGA–TANi colloidal particles, prepared by a self-assembly method, were then coated on Mg alloys by electrophoretic deposition. The prepared γ -PGA–TANi coatings showed a homogeneous surface and good adhesion on AZ31 Mg alloys. Electrochemical analysis and in vitro immersion tests revealed that γ -PGA–TANi-coated Mg exhibited superior corrosion resistance, which was mainly attributed to the electroactivity of the γ -PGA–TANi coatings, leading to the rapid formation of a passivation layer to prevent the further corrosion of Mg alloys. Furthermore, the γ -PGA–TANi alloy coatings efficiently regulated Mg 2+ release and pH value of coated Mg alloy during immersion for 40 days. In comparison with bare Mg alloy, the coated Mg alloy also showed better cytocompatibility, where L929 cells emerged with a good cell adhesion and viability. These results indicate that electroactive γ -PGA–TANi coatings have promising potential for corrosion resistance of Mg alloys. © 2022 Society of Industrial Chemistry.
研究领域:
材料科学
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生物医学工程
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腐蚀科学
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表面工程
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Lin Wang,Yu Pang,Meihua Xin,Mingchun Li,Lulu Shi,Yangfan Mao
In this study, three types of dodecyl chitosan quaternary ammonium salts, each with different spacer groups were synthesized. These chitosan derivatives are N′ , N′ -dimethyl- N′- dodecyl-ammonium chloride- N -amino-acetyl chitosan (DMDAC), N′- dodecyl- N -isonicotinyl chitosan chloride (DINCC) and N′ , N′ -dimethyl- N′- dodecyl-ammonium chloride- N -benzoyl chitosan (DMDBC). The synthesized products were characterized using Fourier transform infrared spectrometers, nuclear magnetic resonance, thermogravimetric analysis, and elemental analysis. The antibacterial and antibiofilm activities against Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ) were investigated. The experimental results indicated that the introduction of hydrophobic groups of spacer groups could enhance the antibacterial and antibiofilm activities of the chitosan derivatives. The antibacterial rates of the chitosan derivatives were over 90 % for both E. coli and S. aureus at a concentration of 0.5 mg/mL. The chitosan derivatives removed >50 % of the mature biofilm of E. coli and over 90 % of the mature biofilm of S. aureus at a concentration of 2.5 mg/mL. Further, the synthesized chitosan derivatives were determined to be non-toxic to L929 cells. Among them, DMDBC exhibited the most promising overall performance and show potential for wide-ranging applications in food preservation, disinfectants, medical, and other fields.
研究领域:
材料科学
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生物技术
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微生物学
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2012
·
IF 3.456
Junping Zeng,Haitao Huang,Shiyuan Liu,Haibo Xu,Jin Huang,Jiahui Yu
This research is aimed to develop a kind of hollow nanosphere based on β-cyclodextrin-grafted α, β-poly(aspartic acid) (β-CD- graft -PAsp) as drug carrier to enhance the stability of camptothecin (CPT) in aqueous media. Firstly, mono(6-(2-aminoethyl) amino-6-deoxy)-β-cyclodextrin (β-CDen) was synthesized by a substitution reaction between mono-6-deoxy-6-(p-tolylsulfonyl)-β-cyclodextrin (6-OTs-β-CD) and ethylenediamine; and then, the five-member rings in poly( l -succinimide) (PSI) were successively opened by β-CDen to obtain β-CD- graft -PAsp. The synthesized β-CD- graft -PAsp was found to form the unique hollow nanosphere with the internal hole of about 17 nm and many β-CD cavities of 0.7 nm interspersed on the shell. The drug-loading behavior of the hollow nanosphere was also evaluated by using CPT as guest molecule of β-CD. It was worth of note that the β-CD- graft -PAsp hollow nanosphere as carrier enhanced the stability of CPT in aqueous media, and the CPT from the β-CD- graft -PAsp hollow nanosphere displayed a sustained release profile and hence resulted in the essential decrease of cytotoxicity to L929 cell line. Furthermore, almost no cytotoxicity of the β-CD- graft -PAsp is desirable for the application of drug carrier. As a result, the β-CD- graft -PAsp hollow nanosphere showed a great potential as the carrier of CPT.
研究领域:
药学
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纳米技术
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材料科学
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药物递送系统
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产品关联
DOI
PubMed
高影响因子
ACS Applied Bio Materials
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2020
·
IF 0
Faming Wang,Pengfei Ren,Katrien V. Bernaerts,Yifu Fu,Wanjun Hu,Naizhen Zhou,Tianzhu Zhang
As one of the nonenzymatic cell-harvesting technologies, a thermal-responsive surface based on poly(2-oxazoline)s has achieved initial success in supporting the adhesion and thermal-induced detachment of animal cells. However, because of the laborious preparation procedure, this technique was only limited to research purposes. In this work, through using poly(glycidyl methacrylate) (PGMA) as the anchor layer, poly(2-propyl-2-oxazoline)s (PPOx) were grafted onto glass wafers through a facile two-step coating and annealing procedure for nonenzymatic cell harvesting. In the first step, the piranha solution-activated glass wafers were immersed into the chloroform solution of PGMA and then annealed for a given period of time to immobilize PGMA onto the glass wafers through the bonding between epoxy groups and hydroxyl groups. In the second step, the PGMA-coated glass wafers were further immersed into the chloroform solution of carboxyl-functionalized PPOx. After annealing, PPOx were immobilized onto the PGMA layer through the bonding between carboxyl groups and the residual epoxy groups. Atomic force microscopy, X-ray photoelectron spectroscopy, and ellipsometry were used to characterize the modified glass wafers. The results of cytocompatibility evaluation showed that the PPOx-coated glass wafers were almost nontoxic and were able to support the adhesion and proliferation of L929 cells well. By lowering the temperature to 8 °C, L929 and Vero cells were successfully detached from the PPOx-coated glass wafers without any enzymatic treatment. Further cultivation has demonstrated that the cooling procedure had little effect on cell viability, and the cells still retained good viability after harvesting.
研究领域:
材料科学
·
生物技术
·
细胞生物学
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表面化学
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产品关联
DOI
PubMed
EUROPEAN POLYMER JOURNAL
·
2018
·
IF 3.741
Yu Zhang,Pan He,Xinming Liu,Hongyu Zhang,Huailin Yang,Chunsheng Xiao,Xuesi Chen
Synthetic polymers that can change their physicochemical properties in response to physiological stimuli have attracted particular interest for constructing smart drug delivery systems. In the present work, a novel oxidation-responsive block copolymer bearing thioether pendants was designed and synthesized by ring-opening alternating copolymerization (ROAP) of phthalic anhydride (PA) and 2-(methylthio)ethyl glycidyl ether (MTG) monomer using mPEG-OH as the macroinitiator and a phosphazene base, t -BuP 1 , as the catalyst. The obtained mPEG- b -P(PA- alt -MTG) copolymer could self-assemble into spherical micelles in water with an average size of about 30 nm and a critical micelle concentration value of 7.44 × 10 −3 mg/mL. 1 H NMR results indicated that the hydrophobic thioether group can be selectively oxidized to hydrophilic sulfoxide by H 2 O 2 , leading to disassociation of the micelles as illustrated by the TEM observation. Nile Red, as a model drug, was loaded into the copolymer micelles. The intensity of the fluorescence emission decreased sharply after addition of H 2 O 2 , indicating an oxidation-triggered drug release. Moreover, the drug-loaded micelles could be effectively internalized into MCF-7 cells, as indicated by the confocal laser scanning microscopy (CLSM) and flow cytometry (FCM) studies. MTT assay further demonstrated that the synthesized copolymer showed good biocompatibility towards L929 cell, even at a concentration up to 0.5 mg/mL. In sum, this kind of copolymer may have great potential applications in oxidation responsive drug delivery.
研究领域:
材料科学
·
药学
·
聚合物化学
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药物递送系统
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Ling Zhou,Weihua Zhang,Changwen Zhao,Wantai Yang
Developing coating materials with low cytotoxicity and high antimicrobial activity has been recognized as an effective way to prevent medical device-associated infections. In this study, a maleic anhydride terpolymer (PPTM) is synthesized and covalently attached to silicone rubber (SR) surface. The formed coating can be further cross-linked (SPM) through the self-condensation of pendent siloxane groups of terpolymer. No crack or delamination of SPM was observed after 500 cycles of bending and 7 day immersion in deionized water. The sliding friction force of a catheter was reduced by 50% after coating with SPM. The SPM coating without adding any extra antibacterial reagents can kill 99.99% of Staphylococcus aureus and Escherichia coli and also significantly reduce bacterial coverage, while the coating displayed no antimicrobial activity when maleic anhydride groups of SPM were aminated or hydrolyzed. The results of the repeated disinfection tests showed that the SR coated with SPM could maintain 87.3% bactericidal activity within 5 cycles. Furthermore, the SPM coating only imparted slight toxic effect (>85% viability) on L929 cells after 36 h of coculture, which is superior to the coating of aminated SPM conjugated with the antimicrobial peptide E6. The terpolymer containing maleic anhydride units have great potential as a flexible and durable coating against implant infections.
研究领域:
材料科学
·
生物医学工程
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微生物学
·
表面化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 30
·18448-18457
·
IF 3.361
Ying Zhao,Xi He,Han Wang,Jiufu Zhu,Huimin Wang,Yan Zheng,Song Zhu,Zhanchen Cui
Hydrolysis resistance is essential to the durability of the dentin bonding interface. Urushiol is a natural monomer that has been used in different fields over thousands of years but has the disadvantage of a long drying time. In this study, we evaluated a novel photocurable derivative of urushiol as the main monomer for polymerization in dentin adhesive and its effect on hydrolysis resistance. The derivative was characterized by Fourier transform infrared spectroscopy and 1H nuclear magnetic resonance spectroscopy. Compared with the Adper Single Bond 2, the experimentally synthesized adhesives had higher contact angles. In particular, the water sorption/solubility of the experimental samples were significantly lower than that of Adper Single Bond 2. The microtensile bond strengths of the test groups were higher than that of the control group, even after 5000 thermocycles. Cytotoxicity test results showed that adhesives based on the original derivative induced low toxicity to L929 cells. The results of this study may shift the focus of future research to natural monomers and even their derivatives which may perform well in dentistry.
研究领域:
牙科学
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材料科学
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高分子化学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Zexiang Zheng,Xing Yang,Yifan Zhang,Weiqiang Zu,Minna Wen,Taisheng Liu,Changren Zhou,Lihua Li
To achieve the pH-responsive release of metformin in tumor acidic microenvironment, we prepared OHA-Met by covalently grafting metformin (Met) onto oxidized hyaluronic acid (OHA) through imine bonds, and then prepared carboxymethyl chitosan (CMCS)/OHA-Met drug loaded hydrogels. The CMCS/OHA-Met hydrogels showed the in-situ injection performance. At pH = 7.4, the cumulative release rate of metformin from CMCS/OHA-Met20 hydrogel was 42.7 ± 2.6 % in 6 h, and the release tended to balance after 72 h. At pH = 5.5, the release kept constant and the cumulative release rate was 79.3 ± 4.7 % at 6 h, showing good pH-responsive behavior. Metformin induced apoptosis of MCF-7 cells through the caspase 3/PARP pathway. CMCS/OHA-Met20 hydrogel could effectively kill MCF-7 cells, while reducing the cytotoxicity of free metformin to L929 cells. In vivo breast cancer recurrence experiments showed CMCS/OHA-Met20 hydrogel could achieve local injection and pH-responsive smart drug delivery at the tumor resection site, inhibiting breast cancer recurrence. Compared with direct administration, CMCS/OHA-Met20 hydrogel reduced the metformin dosage, frequency of administration and systemic side effects.
研究领域:
药学
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生物医学工程
·
癌症研究
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）