JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
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2017
·Vol. 106
·期 2
·408-418
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IF 3.076
Qian Li,Lihong Sun,Lei Zhang,Zhigang Xu,Yuejun Kang,Peng Xue
Polydimethylsiloxane (PDMS) is a commercialized polymer extensively used in the fabrication of versatile microfluidic microdevices for studies in cell biology and tissue engineering. However, the inherent surface hydrophobicity of PDMS is not optimal for cell culture and thus restrains its applications for investigation of long-term behaviors of fibroblasts and stem cells. To improve the surface biocompatibility of PDMS, a facile technique was developed by modifying the PDMS surface with polydopamine-collagen (COL/PDA) complex. The successful synthesis of COL/PDA was verified through proton nuclear magnetic resonance spectroscopy. Compared to surface coating solely with COL or PDA, the surface wettability was significantly improved on COL/PDA-modified PDMS substrates based on water contact angle characterizations. The modified PDMS surface remarkably enhanced the initial adhesion and long-term proliferation of L929 fibroblasts and tendon stem cells (TSCs). Additionally, the effects of COL/PDA coating on cell viability and apoptosis were further investigated under prolonged incubation. We found that the COL/PDA coating on PDMS resulted in a substantial increase of cell viability compared to native PDMS, and the cell apoptosis was considerably impeded on the modified PDMS. This study demonstrated that COL/PDA coating can effectively enhance the surface biocompatibility of PDMS as verified by the enhanced adhesion and long-term proliferation of L929 fibroblasts and TSCs. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 408–418, 2018.
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高影响因子
Antibiotics-Basel
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2022
·Vol. 11
·期 12
·1737
·
IF 5.222
Fang Zhou,Elie Kostantin,De-Quan Yang,Edward Sacher
Although Ag nanoparticles (NPs) have been widely applied in daily life and in biomedical and industrial fields, there is a demand for Ag-based bimetallic nanoalloys (NAs), such as AgCu and AgFe, due to their enhanced antibacterial efficacy and reduced Ag consumption. In this work, we present a comparison study on the antibacterial efficacy and cytotoxicity rates of Ag NPs and AgCu and AgFe NAs to L929 mouse fibroblast cells using the CCK-8 technique based on the relative cell viability. The concept of the minimum death concentration (MDC) is introduced to estimate the cytotoxicity to the cells. It is found that the minimum inhibitory concentrations (MICs) of the NPs againstE. coliandS. aureusdecrease with the addition of both Cu and Fe. There is a strong correlation between the MDC and MIC, implying that the mechanisms of both antibacterial efficacy and cytotoxicity are similar. The enhanced antibacterial efficacy to bacteria and cytotoxicity toward the cell are attributed to Ag+release. The following order is found for both the MIC and MDC: AgFe < AgCu < Ag NPs. However, there is no cytotoxicity to the L929 cells for AgFe and AgCu NAs at their MIC Ag concentrations againstS. aureus.Keywords:AgCu;AgFe;antibacterial efficacy;cytotoxicity;L929 cells;nanoalloy
研究领域:
材料科学
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生物学
·
医学
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DOI
PubMed
高影响因子
Canadian Respiratory Journal
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2019
·
IF 1.803
Sheng Zuo,Zhen Zhu,Yi Liu,Hong Li,Shuang Song,Shaojun Yin
Aim. The transmembrane chemokine (C-X-C motif) ligand 16 (CXCL16) plays a vital role in the pathogenesis of organ fibrosis, including the liver and kidney. However, the detailed biological function of CXCL16 is still not fully understood in the progression of pulmonary fibrosis (PF). The aim of present study is to examine the function of CXCL16 in PF. Materials and Methods. In this study, we constructed the PF model on mouse by using bleomycin and analyzed the effect of the mouse recombinant protein CXCL16 on mouse lung fibroblast L929 (LF) as well. To further examine the connection between CXCL16 and STAT3 in mouse LF cells, the STAT3 inhibitor AG490 was utilized to inhibit the expression of STAT3. Meanwhile, lipopolysaccharide was used to enhance the phosphorylation of STAT3 (p-STAT3) in mouse LF cells. Results. Our results indicated that the level of CXCL16/CXCR6 was significantly upregulated in the mouse PF model. Moreover, the level of p-STAT3 was also promoted. In addition, the mouse recombinant protein CXCL16 not only contributed to the proliferation of mouse LF cells but also induced the expression of p-STAT3 in LF cells. However, the effect of CXCL16 was deeply abolished by the STAT3 inhibitor AG490 in LF cells. Meanwhile, the antibody of CXCL16 deeply reduced the phosphorylation of STAT3 in lipopolysaccharide (LPS) cultured cells. Conclusions. All these results demonstrated that CXCL16 promoted the phosphorylation of STAT3 and further demonstrated that STAT3 was a critical component in CXCL16/CXCR6 signaling pathway. This research not only enhanced the comprehension of CXCL16 but also indicated its potential value as a target in the treatment for human PF.
研究领域:
肺科学
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分子生物学
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免疫学
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PubMed
高影响因子
MATERIALS LETTERS
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2022
·
IF 3.574
Cunfang Zhang,Xinyan Zhang,Song Chen,Jianan Zhang,Shuaishuai Li,Xiaona Li,Toshiyuki Ikoma,Weiyi Chen
Novel silk fibroin-decorated TiO 2 nanowire microspheres were synthesized through in situ decoration of TiO 2 nanowire microspheres with silk fibroin. They exhibited a hierarchical and fibrous structure with a diameter of 400–500 µm and consisted of numerous nanowires with the diameter of 10–20 nm. After incubation with fibroblast L929 cells, they showed an enhanced biocompatibility due to the presence of silk fibroin in contrast to TiO 2 nanowire microspheres.
研究领域:
材料科学
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生物医学工程
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纳米技术
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DOI
高影响因子
DYES AND PIGMENTS
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2020
·
IF 4.613
Jing-Yang Ni,Xiao-Feng Weng,Ru Sun,Yu-Jie Xu,Jian-Feng Ge
1,8-Naphthalimide is widely applied in the construction of probes because it is easy to be modified. However, 1,8-naphthalimide has been reported rarely in the application of organelle labelling especially lipid droplets (LDs). Herein, three types of amine compounds, aliphatic amine ( a , b ), aromatic amine ( c , d ) and heterocyclic amine ( e , f ), were chosen to construct fluorescent dyes ( 1a - 1f ) with 4-bromo- N -butylnaphthalimide ( 1 ) by Ullmann reaction. The maximum emission wavelength ranged from 490 nm to 550 nm. What's more, 1a-1e showed high fluorescence quantum yields (0.54–0.93) in chloroform and 1e-1f had excellent photostability (>92%). In addition, the cell imaging experiment showed that they could label lipid droplets for HeLa cancer cells and L929 normal cells with extremely low toxicity. This work realizes the classical fluorophore 1,8-naphthalimide to synthesize a series of LDs markers without complex synthesis steps.
研究领域:
化学
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生物学
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材料科学
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
·
IF 5.162
Ting Su,Wei Zhao,Lipeng Wu,Wei Dong,Xiaoliang Qi
With the rapid development of biomedicine applications, more flexible and efficient fabrication strategies are required for drug delivery vehicles. Naturally resourced polymers have gained considerable attention for construction of drug carriers. Here, biocompatible hydrogel reservoir system (named PHG-PDA) based on pullulan and polydopamine (PDA) are designed and fabricated by one-pot incorporation of PDA fibers into pullulan hydrogel (PHG). The structure and performance of the PHG-PDAs can be nicely adjusted by regulating the content of PDA fibers in precursor hydrogel solutions. Crystal violet is used as a drug prototype and introduced into hydrogels via swelling-diffusion approach. We found that the drug loading process followed the pseudo-second-order model and Langmuir isotherm model, while the drug releasing process was pH-responsive, and the cumulative release reached 60.3% and 87% at pH 7.4 and 5.0, respectively. In vitro cytotoxicity studies on L929 cells demonstrated that our hydrogels were nontoxic even in a high concentration (3.4 mg/mL). In sum, these biocompatible PHG-PDAs with tunable physicochemical properties are promising systems for drug delivery.
研究领域:
生物医学工程
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材料科学
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药剂学
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DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL RESEARCH
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2022
·
IF 1.097
Tiandi Ding,HaiJiao Chen,Yan Li,Ying Li,Ying Zhi,Zhiqiang Qu,Qiang Sun,Qingqiang Yao,Bo Liu
Sphingosine kinase is a lipid kinase that catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate. Sphingosine-1-phosphate is a bioactive lipid that regulates biological processes. The overexpression of sphingosine kinases is related to a variety of pathophysiological conditions. For example, SphK1 has been shown to be highly expressed in various cancer cells including ovarian, cervical, colon, stomach, lung, and brain cancer. Inhibition of sphingosine kinases is a promising way to treat diseases such as cancer. Through computer-aided drug design, we have discovered a new SphK1 inhibitor named Amb30572637 (SAMS10). In this report, we describe the discovery process and biological characteristics. In biochemical experiments, SAMS10 shows a prominent inhibitory effect on SphK1, with an IC50 value of 9.8 μM. Subsequent MTT experiments show that SAMS10 has anticancer effects toward A549, SKVO3, A375, and LOVO cell lines and has essentially no cytotoxicity against the healthy cell L929. SAMS10 has significant inhibitory activity against the A549 and LOVO cell lines, with IC50 values of 14.64 and 14.48 μM, respectively. It belongs to a moderately active SphK1 inhibitor with lower anticancer activity than the control compound cisplatin, but the effect of SAMS10 toward SphK1 and its anticancer activity indicate that it is a promising lead compound for the development of effective SphK1 anticancer inhibitors.
研究领域:
药理学
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癌症研究
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药物发现
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生物化学
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分子生物学
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高影响因子
POLYMER INTERNATIONAL
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2022
·Vol. 71
·期 11
·1278-1286
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IF 3.213
Miao Dai,Siyuan Li,Yan Cui,Wei Zhang,Hui Shi,Kai Pan,Wei Wei,Xiaoya Liu,Xiaojie Li
Although magnesium (Mg) and its alloys are promising biodegradable materials for orthopedic implants, rapid and uncontrollable degradation of Mg is still a challenge for clinical applications. In this work, electroactive polymer coatings were prepared to improve the corrosion resistance and cytocompatibility of Mg alloys. Amphiphilic electroactive tetraaniline (TANi)-conjugated poly( γ -glutamic acid) ( γ -PGA) with various TANi modification rates ( γ -PGA–TANi 10 , γ -PGA–TANi 20 , γ -PGA–TANi 27 ) were first synthesized. γ -PGA–TANi colloidal particles, prepared by a self-assembly method, were then coated on Mg alloys by electrophoretic deposition. The prepared γ -PGA–TANi coatings showed a homogeneous surface and good adhesion on AZ31 Mg alloys. Electrochemical analysis and in vitro immersion tests revealed that γ -PGA–TANi-coated Mg exhibited superior corrosion resistance, which was mainly attributed to the electroactivity of the γ -PGA–TANi coatings, leading to the rapid formation of a passivation layer to prevent the further corrosion of Mg alloys. Furthermore, the γ -PGA–TANi alloy coatings efficiently regulated Mg 2+ release and pH value of coated Mg alloy during immersion for 40 days. In comparison with bare Mg alloy, the coated Mg alloy also showed better cytocompatibility, where L929 cells emerged with a good cell adhesion and viability. These results indicate that electroactive γ -PGA–TANi coatings have promising potential for corrosion resistance of Mg alloys. © 2022 Society of Industrial Chemistry.
研究领域:
材料科学
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生物医学工程
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腐蚀科学
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表面工程
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Lin Wang,Yu Pang,Meihua Xin,Mingchun Li,Lulu Shi,Yangfan Mao
In this study, three types of dodecyl chitosan quaternary ammonium salts, each with different spacer groups were synthesized. These chitosan derivatives are N′ , N′ -dimethyl- N′- dodecyl-ammonium chloride- N -amino-acetyl chitosan (DMDAC), N′- dodecyl- N -isonicotinyl chitosan chloride (DINCC) and N′ , N′ -dimethyl- N′- dodecyl-ammonium chloride- N -benzoyl chitosan (DMDBC). The synthesized products were characterized using Fourier transform infrared spectrometers, nuclear magnetic resonance, thermogravimetric analysis, and elemental analysis. The antibacterial and antibiofilm activities against Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ) were investigated. The experimental results indicated that the introduction of hydrophobic groups of spacer groups could enhance the antibacterial and antibiofilm activities of the chitosan derivatives. The antibacterial rates of the chitosan derivatives were over 90 % for both E. coli and S. aureus at a concentration of 0.5 mg/mL. The chitosan derivatives removed >50 % of the mature biofilm of E. coli and over 90 % of the mature biofilm of S. aureus at a concentration of 2.5 mg/mL. Further, the synthesized chitosan derivatives were determined to be non-toxic to L929 cells. Among them, DMDBC exhibited the most promising overall performance and show potential for wide-ranging applications in food preservation, disinfectants, medical, and other fields.
研究领域:
材料科学
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生物技术
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微生物学
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药学
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DOI
PubMed
高影响因子
CARBON
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2024
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IF 10.5
Jian Yu,Xin Huang,Xinhao Chen,Pengyu Hu,Tao Liu,Tingting Zhang,Rui Cheng,Tingting Cui,Jun Li
The abuse of antibiotics leading to bacterial resistance has emerged as a significant impediment to wound healing. Consequently, this study developed antibacterial and anti-inflammatory bi-functional carbon dots (AAB-CDs) as the primary active material for the preparation of hydrogel wound dressings, aiming to promote wound healing. The synthesized AAB-CDs exhibited ultra-small size (2.3 nm), abundant surface functional groups, high Zeta potential (30.9 mV), and excellent biocompatibility. The positively charged AAB-CDs with outstanding antibacterial properties, mitigating the risk of bacterial resistance, while the abundant surface functional groups imparted stable antioxidant performance for sustained wound anti-inflammatory effects. Moreover, in vitro experiments demonstrated that the hydrogel dressings exhibited remarkable antibacterial properties against Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ), and significantly promoted the proliferation of L929 fibroblasts. In vivo experiments further substantiated the feasibility of the hydrogel dressings in promoting wound healing and histological analysis revealed the biological mechanisms underlying the promotion of wound healing. In conclusion, AAB-CDs with robust antibacterial and anti-inflammatory properties hold tremendous potential for promoting wound healing, providing practical insights for the biological application of CDs.
研究领域:
材料科学
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生物医学工程
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药物科学
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伤口愈合
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高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2012
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IF 3.456
Junping Zeng,Haitao Huang,Shiyuan Liu,Haibo Xu,Jin Huang,Jiahui Yu
This research is aimed to develop a kind of hollow nanosphere based on β-cyclodextrin-grafted α, β-poly(aspartic acid) (β-CD- graft -PAsp) as drug carrier to enhance the stability of camptothecin (CPT) in aqueous media. Firstly, mono(6-(2-aminoethyl) amino-6-deoxy)-β-cyclodextrin (β-CDen) was synthesized by a substitution reaction between mono-6-deoxy-6-(p-tolylsulfonyl)-β-cyclodextrin (6-OTs-β-CD) and ethylenediamine; and then, the five-member rings in poly( l -succinimide) (PSI) were successively opened by β-CDen to obtain β-CD- graft -PAsp. The synthesized β-CD- graft -PAsp was found to form the unique hollow nanosphere with the internal hole of about 17 nm and many β-CD cavities of 0.7 nm interspersed on the shell. The drug-loading behavior of the hollow nanosphere was also evaluated by using CPT as guest molecule of β-CD. It was worth of note that the β-CD- graft -PAsp hollow nanosphere as carrier enhanced the stability of CPT in aqueous media, and the CPT from the β-CD- graft -PAsp hollow nanosphere displayed a sustained release profile and hence resulted in the essential decrease of cytotoxicity to L929 cell line. Furthermore, almost no cytotoxicity of the β-CD- graft -PAsp is desirable for the application of drug carrier. As a result, the β-CD- graft -PAsp hollow nanosphere showed a great potential as the carrier of CPT.
研究领域:
药学
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纳米技术
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材料科学
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药物递送系统
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产品关联
DOI
PubMed
高影响因子
ACS Applied Bio Materials
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2020
·
IF 0
Faming Wang,Pengfei Ren,Katrien V. Bernaerts,Yifu Fu,Wanjun Hu,Naizhen Zhou,Tianzhu Zhang
As one of the nonenzymatic cell-harvesting technologies, a thermal-responsive surface based on poly(2-oxazoline)s has achieved initial success in supporting the adhesion and thermal-induced detachment of animal cells. However, because of the laborious preparation procedure, this technique was only limited to research purposes. In this work, through using poly(glycidyl methacrylate) (PGMA) as the anchor layer, poly(2-propyl-2-oxazoline)s (PPOx) were grafted onto glass wafers through a facile two-step coating and annealing procedure for nonenzymatic cell harvesting. In the first step, the piranha solution-activated glass wafers were immersed into the chloroform solution of PGMA and then annealed for a given period of time to immobilize PGMA onto the glass wafers through the bonding between epoxy groups and hydroxyl groups. In the second step, the PGMA-coated glass wafers were further immersed into the chloroform solution of carboxyl-functionalized PPOx. After annealing, PPOx were immobilized onto the PGMA layer through the bonding between carboxyl groups and the residual epoxy groups. Atomic force microscopy, X-ray photoelectron spectroscopy, and ellipsometry were used to characterize the modified glass wafers. The results of cytocompatibility evaluation showed that the PPOx-coated glass wafers were almost nontoxic and were able to support the adhesion and proliferation of L929 cells well. By lowering the temperature to 8 °C, L929 and Vero cells were successfully detached from the PPOx-coated glass wafers without any enzymatic treatment. Further cultivation has demonstrated that the cooling procedure had little effect on cell viability, and the cells still retained good viability after harvesting.
研究领域:
材料科学
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生物技术
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细胞生物学
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表面化学
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产品关联
DOI
PubMed
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