ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Jinhui Liu,Jiasen Cui,Xing Wei,Weitao Li,Chunran Liu,Xuewen Li,Mingli Chen,Yu Fan,Jianhua Wang
It is the first time to investigate local distribution patterns of mercury (Hg) in mice organs after Hg and Se exposure with detection of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Two batch of adult mice were employed to be exposed to inorganic mercury (iHg) and methylmercury (MeHg) with or without Se at the dose of 55 μmol kg −1 . Tissue sections of brain, kidney, liver, and spleen from one batch mice were prepared to get local imaging of Hg by LA-ICP-MS. Tissues from another batch mice were used to quantify Hg and Se in tissues with ICP-MS after acid digestion . The results indicated that, for mice exposed to iHg, Hg mainly distributed in kidney, a little in liver, and hardly in brain and spleen; for mice exposed to MeHg, lower amount of Hg was found in kidney, liver and spleen, and almost no Hg was found in brain. It was interesting that for Hg and Se co-administration groups, higher level of Hg was observed in kidney, liver, spleen and even in brain than single Hg administration groups. In addition, Se level in organ tissues increased obviously not only in Se exposure group but also in MeHg exposure group, while the phenomenon was not observed in iHg exposure group. HepG2 cells were employed to investigate Se and Hg interactions in single cell level, similar bioaccumulation behavior of Hg was found between cells and mice organs. Higher level of Hg was observed in cells cultured with Se and Hg medium than cells cultured with single Hg medium. The results are expected to provide new insight to investigate Hg and Se interactions in animal bodies and in-vitro cells.
研究领域:
环境科学
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毒理学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Feng Zhang,Sen Zhang,Xing Liu,Wei Gao,Jun Luo,Yi-fan Pan,Wei-Juan Zheng,Xin Hu,Hong-zhen Lian
Background Glycoproteins are critical in various biological processes and attract lots of attention of researchers. It still remains a challenge to detect glycopeptides by mass spectrometry because of their low abundance with heterogeneity and lability, as well as signal suppression by non-glycopeptides. The boronate affinity-based enrichment is an effective solution for detecting cis -diols-containing compounds including glycopeptides in complex matrices. However, there has not been any report about absolute quantitation of boron in the absorbent materials for glycopeptides, which obstructed the tailored design of boronate affinity materials and deeper understanding of interaction mechanisms. Results This work presents the development of a boronic acid-functionalized magnetic zirconium metal-organic framework (mMOF–AAPBA) with actual boron content for specific capture of glycopeptides. The synthesized nanocomposite could enrich glycopeptides by magnetic solid-phase extraction (MSPE) via the synergistic effect of hydrophilic interaction and boronate affinity. After full characterization including quantitative analysis of boron by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), mMOF–AAPBA was applied as MSPE absorbent to capture glycopeptides. Under the optimized conditions, it could enrich 28 glycopeptides both from horseradish peroxidase and immunoglobulin digests, with a limit of detection of 1 fmol μL −1 coupled with matrix-assisted laser desorption/ionization time of flight mass spectrometry. The boronate affinity absorbent also possessed great ability of anti-interference and good reusability. Furthermore, it possessed high performance in enriching glycopeptides in real samples including human saliva and traditional Chinese medicine injections. Significance In the preparation of the boron-containing nanocomposites, there is a special interest that an accurate protocol was established to assay boron based on LA-ICP-MS assisted by home-made matrix reference substances for the first time. These findings highlight the quantitative boron-containing mMOF–AAPBA as a robust affinity material for glycopeptide enrichment in real biological samples, offering potential applications in glycoproteomics and other cis -diols compound related researches.
研究领域:
分析化学
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生物化学
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纳米技术
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质谱学
·
糖蛋白质组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
GEOSTANDARDS AND GEOANALYTICAL RESEARCH
·
2018
·Vol. 43
·期 1
·93-111
·
IF 3.929
Shenyang Y. Zhang,Hongluo L. Zhang,Zhenhui Hou,Dmitri A. Ionov,Fang Huang
A rapid sample preparation procedure is described to determine trace element compositions of peridotites using LA-ICP-MS. Peridotite powders were fused with albite in a molybdenum–graphite assembly to obtain homogeneous glasses. Best conditions for the fusion procedure (heating at 1500–1550 °C for 10–15 min with a sample-to-flux ratio of 1:2) were constrained with melting experiments on two USGS reference materials, PCC-1 and DTS-2B. Mass fractions of first series transition elements, Ba and Pb, in quenched glasses of PCC-1 and DTS-2B are consistent with published data within 10% RSD. Three spinel peridotite xenoliths from eastern China were analysed following both our method and conventional solution ICP-MS. Compared with solution ICP-MS, the relative deviations of our method for most elements were within 10%, while for the REE, Ta, Pb, Th and U, the relative deviations were within 20%. In particular, volatile elements (e.g., Pb and Zn) are retained in the glass. Compared with conventional wet chemistry digestion, our method is faster. Additional advantages are complete sample fusion, especially useful for samples with acid-resistant minerals (spinel and rutile), and long-term conservation of glasses allowing unlimited repeated measurements with microbeam techniques. The same approach can be used for analyses of other mantle rocks, such as eclogites and pyroxenites.
研究领域:
地球化学
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矿物学
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Zewei Cui,Man He,Beibei Chen,Bin Hu
Due to the enormous interest in plants related to bioscience, environmental and toxicological research, analytical methods are expected with the ability of getting information on elemental transfer, distribution and contents in plants. In this work, a mixture of gelatin (GA) and hydroxypropyl methyl cellulose (HPMC) was prepared to simulate plant matrix, a method based on laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) with matrix-matching external calibration was proposed for direct quantification of multiple elements in plants. The composition of GA&HPMC substrate was optimized, such as the concentration of spiked nitric acid , the mass fraction of both GA and HPMC in the substrate and the mass ratio of GA: HPMC. After spiking elemental solution, coating the mixture onto a glass slide and drying overnight at room temperature , GA&HPMC substrate was obtained. The substrate obtained with GA: HPMC of 8: 2 was used to fabricate the standard series, which exhibited good elemental homogeneity and similar elemental signal intensities in LA-ICP-MS detection to that obtained for plant Certified Reference Material (CRM). CRMs of different plants including Citrus leaf (GBW10019), Tea (GBW07605), Beans (GBW10021) and Scallions (GBW10049) were further pressed into pellets and subjected to the proposed method, and the quantification accuracy was demonstrated. The limits of detections of this method were found to be 0.003 (Ce)-104 (Ca) μg g −1 , with a wide linear range (0.01–10000 μg g −1 ) for 17 target elements. The application potential of the method was further demonstrated by performing elemental imaging in Trigonotis peduncularis leaves. Rapid in-situ quantitative imaging of Zn, Cu, Sr and Mn was achieved, and the elemental quantitative distributions were discussed. The constructed substrate helped direct elemental quantification in plants. It provided a powerful and efficient tool for the investigation of the distribution and transfer of elements in plants, favoring further exploration of elemental bioavailability , transport and toxicity mechanisms.
研究领域:
植物学
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环境科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2022
·
IF 6.556
Wenxin Cui,Zhaoqing Cai,Qing Li,Haiyun Qu,Jiaqian Zheng,Dengguang Yu,Junfeng Chen,Zheng Wang
In this study, a laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) method for in-situ determination of yttrium and trace elements in yttrium-doped barium fluoride (BaF 2 : Y) crystals was proposed. A facile, micro-damage procedure for quantifying the segregation coefficient of doping elements was investigated, and it was found that the actual yttrium doping concentration increases from the seed end to the tail end in BaF 2 : Y crystals. In micro-area analysis, this method has higher mass sensitivity which was applied to quantify the impurity content and distribution during the growth of BaF 2 : Y crystals. Regression coefficient of calibration curve for each element ranged from 0.9918 to 0.9995. Detection limits (DLs) were 0.05, 0.03, 0.01 and 0.01 μg g −1 for Mg, Zn, Sr and Pb, respectively. The accuracy of the proposed method was verified by inductively coupled plasma mass spectrometry/atomic emission spectroscopy (ICP-MS/AES) with wet-chemical pretreatment. The objective of the presented work was to provide a less damaging and more novelty approach for crystal sample analysis.
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
·
2020
·Vol. 35
·期 5
·886-895
·
IF 3.498
Yuqiu Ke,Jianzong Zhou,Xiaoqing Yi,Yijian Sun,Jianfeng Shao,Shiyong You,Wei Wang,Yunzhi Tang,Chaoyang Tu
Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been widely used for in situ microanalysis of elemental concentrations in solid samples, but its application is hampered by a lack of matrix-matched and homogeneous reference materials (RMs). In this work, a new method for preparing matrix-matched and homogeneous LA-ICP-MS reference materials has been developed. A multiple rare earth element (REE) doped CaWO4 single crystal (CaW-1) was successfully prepared by the Czochralski technique. The matrix composition and crystal structure were confirmed by X-ray diffraction (XRD) and electron microprobe analysis (EMPA), which indicates that these crystals have a matrix composition of CaWO4 and are matrix-matched with natural scheelite. The homogeneity of REE distribution was systematically studied by seven methods and the results demonstrated that REEs are distributed homogeneously in the CaWO4 single crystal with slight fluctuation (RSDs: ca. 4%). It can be concluded that the synthesized CaWO4 single crystal meets both crucial requirements, matrix-matched and homogeneous distribution, for an LA-ICP-MS reference material, and therefore it was used to quantitatively calibrate REE concentrations in natural scheelite. The results showed that acceptable analytical errors (<8%) were obtained, which were more desirable than those obtained with non-matrix-matched NIST SRM 610 as the RM. It robustly validates the ability of the developed method for preparing CaWO4 single crystals as possible LA-ICP-MS reference materials to calibrate REE concentrations in natural scheelite.
研究领域:
矿物学
·
分析化学
✓ 已验证
DOI
高影响因子
OPTICAL MATERIALS
·
2019
·
IF 2.687
Nan Jiang,Yu Zhao,Zhiwei Zhu,Wenxue Li,Haiyun Qu,Haohong Chen,Lin Ge,Tengfei Xie,Qiang Liu,Yubai Pan,Jiang Li
Planar waveguide LuAG/10 at.%Yb:LuAG/LuAG laser ceramics were fabricated by the tape casting method combined with the vacuum pre-sintering and hot isostatic pressing (HIP) post-treatment. The morphologies of the raw materials, tapes and green bodies were observed. The microstructure, optical transmittance and the Yb 3+ ions distribution of the LuAG/10 at.%Yb:LuAG/LuAG ceramics with different pre-sintering temperatures after HIP post-treatment were mainly studied. The experimental results showed that the average grain size and in-line transmittance of the HIPed ceramics increased with the increase of pre-sintering temperature. For the LuAG/10 at.%Yb:LuAG/LuAG ceramics pre-sintered at 1825 °C for 20 h and then HIPed at 1700 °C for 3 h under 200 MPa Ar atmosphere, the in-line transmittance of the sample with the thickness of 2 mm was about 74% at 1100 nm and the maximum diffusion distance of Yb 3+ was about 166 μm. The bulk and grain boundary diffusion coefficients of Yb 3+ ions for the samples pre-sintered at 1775 °C–1825 °C after HIP post-treatment were calculated by Fick's second law by using the LA-ICP-MS. What's more, CW laser at 1030 nm was demonstrated and the maximum output power of 0.4 W with a slop efficiency of 9.4% was achieved.
研究领域:
材料科学
·
光学
·
激光物理
·
陶瓷
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）