LWT-FOOD SCIENCE AND TECHNOLOGY
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2021
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IF 4.952
Zhaoxia Yang,Ruohan Wu,Xiaohan Wei,Zuoli Zhang,Wenqing Wang,Aoxue Liu,Jing Yang,Chaofan Ji,Huipeng Liang,Sufang Zhang,Xinping Lin
Little is known about the relationship between the chemical and microbial properties of Chouguiyu, a traditional Chinese fermented fish with unique aroma and plentiful taste. Therefore, we explored this relationship by determining the bacterial and fungal distribution of 14 brands of Chouguiyu . The distribution of microorganism and their effect on product quality, such as volatile flavor compounds (VFCs), free amino acids (FAA), physicochemical features and organoleptic properties were evaluated. Five VFCs (2-ethyl-1-hexanol, eucalyptol, 1-octen-3-ol, nonanal and indole) and three FAAs (Glu, Vla and Ala) were considered as core components. LAB ( Vagococcus and Lactococcus ) were found to contribute greatly to critical aroma formation and inhibition of TVB-N, leading to a moderate fermentation and good quality. While excessive increases in TVB-N, VFCs and FAA contents were found to be related with Psychrilyobacter and Fusobacterium , leading to poor organoleptic properties. The addition of Sufu to these products may be associated with the accelerating the fermentation process. Moderate fermentation with LAB is beneficial to typical aroma and good organoleptic properties, while excessive fermentation related with Psychrilyobacter and Fusobacterium may lead to overpowering flavors and lowered organoleptic properties.
研究领域:
食品科学
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微生物学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2025
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IF 7.7
Hong Luo,Yifan Yang,Gongjie Xu,Bin Cai
Accurate detection of molecules at ultra-low concentrations is essential for applications such as environmental monitoring, early-stage diagnostics, and precision medicine. Among various sensing platforms, optical fiber and waveguide-based systems are particularly valuable due to their immunity to electromagnetic interference, absence of electrical hazards, flexibility, and low attenuation over long distances. Lab-on-a-fiber (LoF) integrates sensing elements directly onto optical fibers, transforming them into versatile platforms for diverse sensing applications. However, LoF faces limitations in sensitivity due to the reliance on surface-adsorbed analytes and weak interactions with evanescent waves. Lab-in-a-fiber (LiF) sensors improve sensitivity by modifying the fiber’s geometry to create microfluidic channels, but they are hindered by significant optical losses and structural complexity. To overcome these challenges, we propose a novel LiF sensor by coaxially grafting a hydrogel waveguide with optical fibers (LiF-hydrogel). This hybrid structure combines the advantages of conventional waveguides with the infiltrability properties of hydrogels, allowing analyte molecules to penetrate the waveguide and enhancing sensitivity. This design achieves an exceptionally low detection limit for the fluorescent spectrum of rhodamine B (Rh B), reaching approximately 2.0 × 10 −20 M, equivalent to the detection of only 4–6 individual Rh B molecules. Furthermore, by embedding Rh B-lactam-ethylenediamine within the hydrogel waveguide, the LiF-hydrogel can selectively detect mercury (Hg 2+ ) ions with a detection limit of approximately 1.0 × 10 −13 M in aqueous solutions, underscoring its remarkable sensitivity.
研究领域:
光学传感
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生物传感器
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环境监测
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分析化学
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纳米技术
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光子学
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高影响因子
ADVANCED MATERIALS
·
2024
·Vol. 37
·期 4
·2411510
·
IF 27.4
Tiansheng Huang,Li-Peng Sun,Xiangping Li,Bai-Ou Guan
Despite the great success in deploying metal-organic frameworks (MOFs) as efficient electrocatalysts, the low adoption of operando methods hinders the understanding of underlying mechanism. By leveraging the subtle refractive index evolution, including both the real and the imaginary parts, an entirely new concept of a lab-on-fiber operando method and its feasibility for “pristine-immersion-operando-post analysis” of electrocatalyts are demonstrated. Concurrent collection of absorption spectra and surface plasmon resonance is achieved by engineering fiber-optic waveguides to simultaneously induce guided light attenuation and plasmonic coupling. In situ-formed Co hydroxide and oxide reactive intermediates in zeolitic imidazolate framework-67 (ZIF-67) electrocatalyst are optically identified, which shows its underlying self-reconstruction conversions at different stages during electrocatalytic oxygen evolution reactions, and address the gap in knowledge concerning whether ZIF-67 is a precatalyst for real catalyst production. This illuminating operando method offers intriguing opportunities to collect all the observables in a single fiber, provides an exciting potential of a new class of device with long-sought integration and miniaturization capability, and is expected to enable the electrocatalysis community to conquer challenges with conducting multimodal operando experiments outside the laboratory.
研究领域:
材料科学
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电化学
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光学工程
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DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2021
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IF 6.475
Daqun Liu,Chengcheng Zhang,Jianming Zhang,Xiaoting Xin,Xiaojun Liao
Fermented vegetable flavors are closely associated with microbial metabolism. Here, shifts in flavor metabolites and their correlations to the structure and function of fermentative microbial communities were explored during the spontaneous fermentation process of potherb mustard ( Brassica juncea var. multiceps ), a traditionally fermented vegetable from China. Halophilic bacteria (HAB, i.e. , Halomonas , Salinivibrio , and Vibrio ) and lactic acid bacteria (LAB, i.e. , Lactobacillus -related genera and Weissella ) became highly abundant after potherb mustard fermentation. Further, HAB and LAB abundances exhibited significant, positive correlations with metabolites important in fermented potherb mustard flavoring (e.g., organic acids, amino acids, alcohols, aldehydes, and nitriles). Metagenomic analysis indicated that Halomonas , Salinivibrio , Weissella , and Lactobacillus -related genera were likely actively engaged in pyruvate metabolism (ko00620) and citrate cycle (TCA cycle, ko00020), leading to higher lactic and acetic acid concentrations, along with lower pH, which would affect levels of volatile isothiocyanates and nitriles that contribute to flavoring of fermented potherb mustard. Further, HAB and LAB were the primary populations inferred to be responsible for amino acid and fatty acid metabolism in addition to the biosynthesis of numerous volatile flavor compounds. This study highlights the predominance and importance of LAB and HAB during spontaneous fermentation of potherb mustard and provides new insights into their roles in this process.
研究领域:
微生物学
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食品科学
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代谢组学
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DOI
PubMed
高影响因子
ACS Nano
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2025
·
IF 16
Mohammad Zaki,Carsten Prinz,Bastian Ruehle
The recent emergence of self-driving laboratories (SDL) and material acceleration platforms (MAPs) demonstrates the ability of these systems to change the way chemistry and material syntheses will be performed in the future. Especially in conjunction with nano- and advanced materials which are generally recognized for their great potential in solving current material science challenges, such systems can make disrupting contributions. Here, we describe in detail MINERVA, an SDL specifically built and designed for the synthesis, purification, and in line characterization of nano- and advanced materials. By fully automating these three process steps for seven different materials from five representative, completely different classes of nano- and advanced materials (metal, metal oxide, silica, metal organic framework, and core–shell particles) that follow different reaction mechanisms, we demonstrate the great versatility and flexibility of the platform. We further study the reproducibility and particle size distributions of these seven representative materials in depth and show the excellent performance of the platform when synthesizing these material classes. Lastly, we discuss the design considerations as well as the hardware and software components that went into building the platform and make all of the components publicly available.
研究领域:
材料科学
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化学工程
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人工智能
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2020
·
IF 10.257
Rui Jin,Lianjing Zhao,Xu Yan,Xiaosong Han,Mengqi Liu,Yue Chen,Qingyun Li,Dandan Su,Fangmeng Liu,Peng Sun,Xiaomin Liu,Chenguang Wang,Geyu Lu
Oxalate is commonly employed as adjuvant of pesticide agent, causing renal injury of human even in trace residues. Despite the great achievements of the existing point-of-care testing (POCT) technology, accurate on-site screening of oxalate remains a tricky issue. To this aim, we proposed a “lab in a tube” platform which integrated portable hydrogel kit with smartphone for real-time monitoring of oxalate to achieve quantitatively precise analysis. In this work, a stimuli-responsive hydrogel-based kit was constructed via embedding manganese dioxide (MnO 2 ) nanosheets into sodium alginate hydrogel system. Based on the intrinsic oxidase-like activity, MnO 2 nanosheets-based nanozyme triggered color reaction by introducing a common sensing probe 3,3′,5,5′-tetramethylbenzidine. Meanwhile, the presence of oxalate would decompose MnO 2 nanosheets, inducing the decrease of nanozyme activity, which resulted in the color response of portable kit. Coupling with ImageJ software, the image information of kit captured via smartphone could be transduced into the hue intensity, which provided a directly quantitative tool to detect oxalate with a detection limit of 8.0 μmol L −1 . This portable smartphone biosensor was successfully applied for screening urine sample within 10 min for high-throughput analysis (twelve samples) without the need for any advanced analytical instruments. Based on the merits of simple operation, cost-efficiency, and good selectivity, the availability of the miniaturized biosensor platform for POCT will achieve the requirements of routine screening and disease prevention.
研究领域:
分析化学
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生物医学工程
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DOI
PubMed
高影响因子
Biomicrofluidics
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2016
·Vol. 10
·期 3
·
IF 2.708
Morton J. A. S.,Bridle H.
Microfluidics has shown rapid growth in both research and development and offers significant commercialisation potential for biomedical and diagnostic applications in particular. However, there is a lack of awareness of microfluidics outside the field of study, and few dedicated educational programmes are available. While many topics incorporate microfluidics teaching, reported initiatives in the literature have not yet taken a problem based learning (PBL) approach to the delivery of practical sessions. The educational approaches already reported typically focus upon production and testing of pre-determined device designs for specific applications, using a “recipe” style of lab teaching. Here, we report on a newly designed lab section of a microfluidic teaching component utilising problem based learning (PBL) to involve the students in all aspects of design, manufacture, and performance characterisation of microfluidic solutions. Details of the lab design and development are given enabling others to replicate the lab structure described here or use it as a basis for the design of similar PBL microfluidics teaching labs. A key focus of the work has been the evaluation of the student experience, and the results of a survey indicate a high degree of student satisfaction and skills development due to the PBL approach.
研究领域:
生物医学工程
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教育学
·
微流控技术
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DOI
PubMed
高影响因子
International Journal of Pavement Engineering
·
2022
·
IF 4.178
Zhiqiang Jiang,Chenhao Tang,Jian Yang,Yujing You,Zhongda Lv
Conventional pavement materials like SMA and GA do not meet the requirement for steel bridge decks. Epoxy asphalt concrete (EAC) has good performances but also has fatigue problems stemming from their inherent chemical structures, which, in some cases, led to short service life. Herein, we report a polyurethane concrete (PUC) consisting of a one-phase tough polyurethane binder as the matrix and dolerite aggregates as filler. First, PUC samples are prepared by mixing liquid reactants like polycarbonate diol, aliphatic isocyanate and glycerine with dolerite aggregates. The optimum formula of the PUC (binder-aggregate ratio of 8.5%) is determined by the Marshall stability and compressive strength tests. Second, Hamburg wheel tracking, freeze-thaw splitting, low temperature bending and linear contraction coefficient measurements are conducted on the PUC samples. Test results show that PUC composites have strong low-temperature toughness (the flexural strength 24.3 ± 1.3 MPa and flexural strain of 7020*10−6 at −10°C) and high dynamic stability (153,000 cycles/mm, about as 5 times as epoxy asphalt concrete (EAC). The alkaline hydrolysis tests show PUC has excellent durability (remained strong after 24 days of hot alkaline water treatment). These results support PUC is a promising material for bridge deck pavement.
研究领域:
土木工程
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材料科学
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DOI
高影响因子
JOURNAL OF CHEMICAL EDUCATION
·
2021
·
IF 2.979
Qiaomei Chen,Yang Yang,Ying Yu,Huaping Xu
This article introduces a novel experiment that involves vitrimers, a new type of polymer, in polymer chemistry and physics lab courses for undergraduate students. Vitrimers are permanently cross-linked networks that can be reprocessed under certain external stimuli. Therefore, they combine the advantages of traditional thermoplastics (excellent reprocessability) with thermosets (outstanding mechanical properties and environmental stability). They can be regarded as a kind of “special thermoset”, which moves this industry toward a more sustainable path, as they are conducive to decreasing thermosetting wastes, reducing environmental pollution, and cutting down the waste of resources. From this lab class, students obtained more experience in the preparation and characterization of polymers. They also learned the concepts of dynamic chemistries and vitrimers and gained a better understanding of the advantages and limitations of thermosets and thermoplastics, as well as a strategy for making a reprocessable thermoset polymeric material. It also fostered students’ interest in functional materials and inspired them to design other functional materials. Moreover, this experimental setup is convenient and safe for implementation and dissemination in most undergraduate laboratory classes.
研究领域:
高分子化学
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材料科学
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化学教育
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DOI
高影响因子
Nutrients
·
2024
·Vol. 16
·期 5
·600
·
IF 4.8
Juan Yang,He Gao,Tiantian Zhang,Yong Fan,Yuwei Wu,Xinyu Zhao,Ying Li,Lei Wu,Hui Zhao,Lingshuang Yang,Haojie Zhong,Longyan Li,Xinqiang Xie,Qingping Wu
Hepatocellular carcinoma (HCC), being ranked as the top fifth most prevalent cancer globally, poses a significant health challenge, with a considerable mortality rate. Hepatitis B virus (HBV) infection stands as the primary factor contributing to HCC, presenting substantial challenges in its treatment. This study aimed to identify lactic acid bacteria (LAB) with anti-HBV properties and evaluate their impact on the intestinal flora in HBV-associated HCC. Initially, two LAB strains,Levilactobacillus brevisSR52-2 (L. brevisSR52-2) andLeviLactobacillus delbrueckiisubsp.bulgaicusQ80 (L. delbrueckiiQ80), exhibiting anti-HBV effects, were screened in vitro from a pool of 498 LAB strains through cell experiments, with extracellular expression levels of 0.58 ± 0.05 and 0.65 ± 0.03, respectively. These strains exhibited the capability of inhibiting the expression of HBeAg and HBsAg. Subsequent in vitro fermentation, conducted under simulated anaerobic conditions mimicking the colon environment, revealed a decrease in pH levels in both the health control (HC) and HCC groups influenced by LAB, with a more pronounced effect observed in the HC group. Additionally, the density of total short-chain fatty acids (SCFAs) significantly increased (p< 0.05) in the HCC group. Analysis of 16S rRNA highlighted differences in the gut microbiota (GM) community structure in cultures treated withL. brevisSR52-2 andL. delbrueckiiQ80. Fecal microflora in normal samples exhibited greater diversity compared to HBV-HCC samples. The HCC group treated with LAB showed a significant increase in the abundance of the phyla Firmicutes, Bacteroidetes and Actinobacteria, while Proteobacteria significantly decreased compared to the untreated HCC group after 48 h. In conclusion, the findings indicate that LAB, specificallyL. brevisSR52-2 andL. delbrueckiiQ80, possessing antiviral properties, contribute to an improvement in gastrointestinal health.
研究领域:
微生物学
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医学研究
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癌症生物学
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病毒学
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胃肠病学
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DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2026
·
IF 9.1
Tianwen Zheng,Kangping Huang,Naijin Wu,Moxi Wang,Liqin Zhi,Xiao Zhang,Yaling Gou,Peizhong Li,Wenxia Wei,Huilong Luo,Xiang Li,Zhongping Sun
Microbial induced calcium carbonate precipitation (MICP) has attracted considerable attention as an environmentally friendly method for the preparation of CaCO 3 . In this paper, the microorganism producing carbonic anhydrase (CA) was selected to prepare biological CaCO 3 , and a 30 L reactor was constructed to enable batch preparation at the laboratory scale. Subsequently, the key influencing factors in the preparation process of biological CaCO 3 were studied based on enzyme activity and Michaelis-Menten equation, and the performances of biological CaCO 3 were explored through various characterization methods. Finally, the preparation mechanism of biological CaCO 3 was revealed. The results of reaction kinetics induced by CA indicated that the Michaelis constant ( K m ) during the formation of biological CaCO 3 was 4.438 mM, and the reaction rate ( V max ) when CA was saturated with substrate was 1.737 mM·h −1 . Moreover, the hydration reaction of CO 2 catalyzed by CA was the key influencing factor in the formation process of biological CaCO 3 . Various test results showed that calcite biological CaCO 3 had a porous structure with a particle size distribution of 3–8 μm, a specific surface area of 83.19 m 2 /g, and an adhesion force of 829 nN. Meanwhile, the microorganism acted as nucleation sites, promoting the nucleation and crystal growth of biological CaCO 3 . In general, this paper provides a new method and approach for the batch preparation of biological CaCO 3 , which is beneficial for advancing its preparation and application in the future
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高影响因子
npj Science of Food
·
2026
·
IF 9.7
Shi Cong-Rong,Zhang Xiao-Juan,Xie Han,Zhang Wei,Tang Rui-Xin,Lu Zhen-Ming,Geng Yan,Lu Ran,Wang Fang-Zhou,Chai Li-Juan,Zhang Hui-Ling,Xu Hong-Yu,Shi Jin-Song,Xu Zheng-Hong
Hyper-osmotic stress in high-sugar fruits inhibits the fermentation performance of lactic acid bacteria (LAB). Besides, there is a prevailing trend of low-sugar diets for health concern. Therefore, it is essential to develop LAB strains with high-sugar tolerance and efficient sugar metabolism. In this study, three high-sugar-adaptive strains (2–12 mutations) were obtained through random mutagenesis and screening at 150 g/L glucose. When applied to high-sugar goji juice fermentation, they reduced glucose from 92.26 to 28.85 g/L, reached 2.56*10 9 CFU/mL, and shifted the sensory profile from sweet and herbal to fruity and pleasantly sour. Metabolomics revealed enrichment of bioactive metabolites such as indole-3-lactate (+22.32%), linoleate (+16.37%) and spermine (+64.50%) in strain LP-4-3*. Mechanistically, high-glucose-adaptive LAB accumulated more maltose, galactitol, and glycosylated phenolic compounds to alleviate osmotic pressure induced by high glucose. This study highlights the potential of high-glucose-adaptive LAB in developing low-sugar and health-promoting fermented fruit juices.
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PubMed
高影响因子
危险品化学品经营许可证（带存储）