Materials Today Bio
·
2025
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IF 10.2
Yi Liao,Shengmei Zhou,Andrew J. Quantock,Wei Li,Yuan Xu,Xie Fang,Robert D. Young,Wanling He,Qingjian Li,Houjian Zhang,Ruochen Wang,Yi Han,Hongwei Cheng,Wenjun Li,Lu Peng,Rongrong Zong,Yi Hong,Zhirong Lin,Zuguo Liu
Although cell-encapsulating hydrogels are of tremendous interest in regenerative medicine, few of them have been used in clinics and rarely used natural extracellular matrices as polymer precursors. One successful example is to use riboflavin (RF)/ultraviolet A (UVA) to cross-link corneal collagen, which has been used in clinics to halt disease progression in patients with corneal ectatic diseases. However, high-energy UVA and its action on RF cause tissue damage, particularly irreversible endothelium loss, and thus standard RF/UVA protocol may have limitations in treating patients at the advanced stage with thin cornea. Photo-initiators lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) has high efficiency under visible 405 nm light, and is widely applied in 3D bioprinting to cross-link synthesized monomers. Here, we introduced a new strategy to cross-link cornea using LAP and visible light (VL). The LAP/VL protocol could effectively increase corneal stiffness with equivalent efficiency to the RF/UVA protocol in both porcine and rabbit cornea. As LAP and VL were used, the loss of corneal endothelial and stromal cells was minimized, and epithelial wound healing and stromal cell repopulation were accelerated. In summary, we propose that the LAP/VL protocol is an effective and safe alternate for cornea CXL with advantages for relatively thin cornea. Our study also expands the application scope of LAP, indicating it is a suitable photocatalyst for in situ natural extracellular matrices CXL.
研究领域:
眼科学
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再生医学
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生物材料
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光生物学
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组织工程
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DOI
PubMed
高影响因子
Alexandria Engineering Journal
·
2024
·
IF 6.2
Huiyuan Peng,Weihua Guo,Jinsong Wang,Weili Xia,Yu Xiong,Wenhua Xu,Weibo Dai,Feng Zheng
Addressing the devastating and often irreversible effects of stroke necessitates innovative therapeutic strategies that go beyond the limitations of current treatments. This study heralds a promising interdisciplinary approach by employing LAP/PDA composite nanomaterials integrated into silk fibroin-based hydrogels. These engineered materials not only demonstrated significantly enhanced mechanical properties, but also showed excellent biocompatibility when co-cultured with human mesenchymal stem cells (hMSCs). Importantly, the application of these composite hydrogels in stroke models yielded remarkable therapeutic outcomes. The results indicated a substantial reduction in cerebral infarction volumes, improved survival rates of transplanted hMSCs, and increased neurogenesis, underlining their transformative potential in stroke management. Molecular markers such as AQP4, α-DG, and β-DG were also explored, revealing modulated astrocyte activation and cellular signaling pathways. In summary, the composite LAP/PDA nanomaterials, in conjunction with hMSCs and Brain-Derived Neurotrophic Factor (BDNF), unveil a compelling and multifaceted approach for addressing the unmet needs in stroke treatment, thereby opening new avenues in regenerative medicine and tissue engineering.
研究领域:
神经科学
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再生医学
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组织工程
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纳米技术
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干细胞生物学
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DOI
高影响因子
Extreme Mechanics Letters
·
2026
·
IF 4.5
Yuan Wang,Yuechen Jiang,Yecheng Wang
Peel and lap shear are commonly used tests for soft materials. Our previous comparison of peel and lap shear for measuring bulk toughness leads to a fundamental question: do peel and lap shear measure identical adhesion toughness? Here we compare peel and lap shear for measuring adhesion toughness and use the adhesion between polyacrylamide hydrogel and polyacrylic acid hydrogel as a model system. To prepare samples, we introduce a precut crack to the adhesion interface of two adhered hydrogels and sandwich the adhered hydrogels between two stiff layers. In both types of tests, we pull one stiff layer at a constant velocity and record the force-displacement curve. In peel, as the displacement increases, the force increases to a peak and subsequently drops to a plateau, the crack propagates steadily in the sample, and the adhesion toughness is determined by the plateau force. In lap shear, as the displacement increases, the force increases to a peak and subsequently drops to zero, the crack propagates unstably in the sample, and the adhesion toughness is determined by the peak force. Our experiments show that the average values of adhesion toughness measured in peel and lap shear are nearly identical. We also prepare hydrogels of different values of pH and measure the adhesion toughness using peel and lap shear. When pH is high, the adhesion is brittle, the crack propagates along the adhesion interface, and adhesive fracture takes place. When pH is low, the adhesion is tough, the crack kinks into the hydrogels, and cohesive fracture takes place. In either case, the average values of adhesion toughness measured in peel and lap shear are comparable. We further measure the adhesion toughness of hydrogels of different crosslink densities, and peel and lap shear give nearly identical adhesion toughness for all the crosslink densities. These results guide the use of peel and lap shear for characterization of adhesion of soft materials.
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高影响因子
ACS Polymers Au
·
2026
·
IF 6.7
Liu Kun,Long Yao,Zhou Jiahao,Tan Haoyun,Han Xupeng,Gao Xihao,Wei Shana,Chen Zhifang,Shang Tingxuan,Tan Jiaping,Liu Meilin,Yi Wenjun,Gu Xiaoxing,Li Lijun
High vinyl polybutadiene (HVPB) liquid rubber has extensive
applications
in multiple fields, such as rubber, coatings, and adhesives. Quantitative
functionalization modification of polybutadiene will further expand
its application scope. In this paper, a classic living anionic polymerization
(LAP) method was adopted, using 1,2-bis(4-ethylpiperazin-1-yl)ethane
(BEPE) as a novel modifier, n -butyllithium as an
initiator, and N,N-dimethylvinylbenzylamine as a functional monomer.
Through an in situ copolymerization mode, the quantitative and chain-middle/end
positioning introduction of tertiary amine functional groups was achieved,
and a series of highly vinyl-functionalized polybutadiene ( f -HVPB) liquid rubbers with controlled molecular weights
and narrow molecular weight distributions were prepared. On the other
hand, m -chloroperbenzoic acid was used as an epoxidizing
agent to quantitatively epoxidize liquid high-vinyl polybutadiene
and functionalized polybutadiene to prepare side-chain highly epoxy/tertiary
amine bifunctional polybutadiene ( bf -HVPB) products.
The effects of different reaction systems, including different solvents,
different epoxidizing agent ratios, different temperatures, different
molecular weights, and amine modification, on the epoxidation of HVPB
and f -HVPB were mainly investigated. The fine structures
of the polymerization products were systematically analyzed by 1 H NMR, FTIR, DSC, and GPC.
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Rihui Su,Gongke Li,Xiaohua Xiao
In this work, Ag nanoparticles (AgNPs) were embedded into a poly(N-isopropylacrylamide)-laponite (Ag/PNIP-LAP) hydrogel membrane for highly sensitive surface-enhancement Raman scattering (SERS) detection. In situ polymerization was initiated by UV light to encapsulate AgNPs in a PNIP-LAP hydrogel to prepare a highly active SERS membrane with a three-dimensional structure. Due to the surface plasmon resonance and high swelling/shrinkage ratio of the Ag/PNIP-LAP hydrogel SERS membrane, its network structure has a “sieving” effect, which makes it easier for hydrophilic small-molecule targets to enter the sterically confined hydrogel, and the AgNPs are close to each other to form a Raman “hot spot” through the shrinkage of the hydrogel, at the same time, the analyte is enriched in the confined space and close to the AgNPs to form a stronger SERS signal. The characterization of the SERS activity of the Ag/PNIP-LAP hydrogel showed that the prepared three-dimensional membrane had high detection sensitivity for urotropine, 2,5-dimethylpyrazine, pyrazinamide, and pyrazine; the detection limits (S/N = 3) were 17.4, 31.0, 53.1, and 1.11 μg/L, and the analytical time was 35 min. Due to the hydrophilicity of the Ag/PNIP-LAP hydrogel membrane, the small molecules can easily enter the SERS membrane, and the hydrophobic macromolecules are blocked outside the SERS membrane. The SERS method has good selectivity, stability, and reproducibility. The SERS method was applied to the detection of urotropine in dried bean curd sticks, 2,5-dimethylpyrazine in nuts and potato chips, and pyrazinamide in human plasma with recoveries of 81.8–116.8% and the relative standard deviation within 4.9–9.9%. The results matched well with that found by the corresponding chromatographic methods. The proposed method has the advantages of simple sample pretreatment, speediness, high sensitivity, and good selectivity to hydrophilic compounds and has potential application in the rapid on-site detection.
研究领域:
分析化学
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材料科学
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食品科学
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生物医学
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产品关联
DOI
PubMed
高影响因子
Carbohydrate Polymer Technologies and Applications
·
2025
·
IF 6.5
Tahirou Sogore,Meimei Guo,Na Sun,Donglei Jiang,Donghong Liu,Mofei Shen,Tian Ding
Cultured meat offers a promising solution to meet global protein demand using stem cells rather than traditional animal agriculture. The production relies on gel-based scaffold materials, requiring photoinitiators like lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) for crosslinking and forming the three-dimensional matrix. However, LAP residues in the final product pose potential health risks, necessitating efficient detection and removal strategies. In this study, we analyzed cultured fish meat samples, revealing LAP residues, then prepared an eco-friendly CD-MOF from edible materials and demonstrated its excellent LAP adsorption capabilities. The adsorption quantity was investigated using high-performance liquid chromatography (HPLC), and the method's versatility was validated by expanding the study to 2-Hydroxy-4′-(2- hydroxyethoxy)-2-methylpropiophenone (Irgacure 2959) and 2,2-dimethoxy-2-phenylacetophenone (Irgacure 651). CD-MOF achieved maximum adsorption rates of 88.19 ± 3.65 mg/g for LAP, 60.88 ± 3.51 mg/g for Irgacure 2959, and 56.52 ± 3.28 mg/g for Irgacure 651. Spiked recoveries in cultured meat samples ranged from 84.36 % to 98.78 %, with RSDs between 2.16 % and 6.87 %, demonstrating high precision. These findings suggest CD-MOF's promise for detecting and removing photoinitiator residues in cultured meat production.
研究领域:
食品科学
·
生物工程
·
材料科学
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产品关联
DOI
高影响因子
COMPOSITES PART A-APPLIED SCIENCE AND MANUFACTURING
·
2021
·
IF 7.664
Guangming Yang,Qin OuYang,Jinrui Ye,Ling Liu
A new carbon nanotube (CNT)/graphene (GN) grid structure is prepared and used to reinforce and health-monitor the epoxy composites. Results reveal that with a 30–50% mesoscopic-porosity of the CNT/GN grid, tensile strength and single-lap-shear (SLS) strength of the CNT/GN/epoxy composites can be improved by 27–41% and 28–49%, respectively. Also, relative resistance change (Δ R / R 0 %) at damage initiation would sudden change more than 10% for the tensile testing and 6% for the SLS testing. Meanwhile, during the damage evolution stage, the gauge factor obtained from tensile testing reaches 10.2–24.9 improved by 23–200%, and Δ R / R 0 % from SLS testing achieves 47.4–60.4% increased by 69–116%, respectively, when compared to the baseline composite. Moreover, an equivalent resistance prediction mode for the CNT/GN grid composites is proposed and which can well explain the resistance response mechanism. The result reveals that the gridded CNT/GN sensor is potential in reinforcing and structural health monitoring the epoxy resin-based composites.
研究领域:
材料科学
·
传感器技术
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Yiqian Ren,Yao Qiang,Beibei Zhu,Wei Tang,Xinrui Duan,Zhengping Li
The abnormally expressed peptidases in human tissues are associated with many kinds of cancers. Monitoring of endogenous peptidase activity could allow us for pathophysiology elucidation and early clinical diagnosis. Herein, we developed a general strategy for bioluminescence (BL) sensing of peptidase activity in vivo based on tumor-targeting probiotics. The probiotic that harbored a luciferase-encoding plasmid was used to target and colonize tumor and provide luciferase for BL imaging. The peptide-based probes Lc and GPc were applied to track leucine aminopeptidase (LAP) and dipeptidyl peptidase IV (DPPIV) activity, respectively, by simply adding l-leucine and Gly-Pro dipeptides at the N-terminus of d-cysteine, which were specifically controlled by peptidase cleavage and released free d-cysteine to conduct a subsequent click condensation reaction with 2-cyano-6-hydroxybenzothiazole (HCBT) to produce firefly luciferin in situ, giving rise to a strong BL signal. Neither gene modification of cells of interest nor complicated synthesis was required in this BL system. Encouraged by these advantages, we successfully used our probes to monitor LAP and DPPIV activities in vitro and in vivo, respectively. A good linearity between BL and peptidase was obtained in the concentration range of 2.5–40.0 mU/mL with a limit of detection (LOD) of 1.1 mU/mL (55 ng/mL) for LAP and 2.0–40.0 mU/mL with a LOD of 0.78 mU/mL (1.15 ng/mL) for DPPIV, respectively. Additionally, approximately 5-fold (LAP) and 10-fold (DPPIV) differences in the BL signal before and after treatment with a specific inhibitor were also obtained for in vivo BL imaging. All these results reflected the potential application value of our probes in BL sensing of peptidase activity. We envision that our strategy may be a useful approach for monitoring a wide range of peptidases in tumors, especially in primary tumors.
研究领域:
生物技术
·
癌症研究
·
分子生物学
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DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2019
·
IF 0
Feiyi Wang,Sisi Hu,Qi Sun,Qiang Fei,Chao Ma,Cuifen Lu,Junqi Nie,Zuxing Chen,Jun Ren,Guo-Rong Chen,Guichun Yang,Xiao-Peng He,Tony D James
Currently, chemotherapy is a widely used and important treatment for cancer. However, almost all of the treatments have shortcomings associated with poor specificity and high toxicity, which results in severe side effects to normal cells and tissue. This is a very important problem, and yet, it currently remains unanswered. Therefore, the development of the method for the more effective delivery of anticancer drugs to their targets and real-time monitoring of the localization of the drugs are very important. Herein, we designed a theranostic prodrug: CPT-p-Leu, which was constructed using fluorescent camptothecin (CPT), a self-immolative linker and leucine (Leu) residue. Upon exposure to LAP (leucine aminopeptidase: LAP), the amide bond in CPT-p-Leu will be cleaved, followed by an intramolecular 1,6-elimination, which triggers the active anticancer drug (CPT) release and recovers the fluorescence of CPT. With our design, the anticancer drug, CPT, can be used as both a drug and a fluorescence reporter, making our system suitable to accurately and effectively track the released CPT distribution. Based on this strategy, CPT-p-Leu could achieve the chemoselective detection of LAP and monitoring of the anticancer drug release. Furthermore, it also provides a very convenient way to accurately determine the location of the released drug in living samples. In addition, CPT-p-Leu shows a good cell membrane permeability and enhanced cytotoxicity toward LAP overexpressing cancer cells. We anticipate that our research will facilitate the development of improved theranostic systems for cancer therapy.
研究领域:
肿瘤学
·
药物化学
·
分子生物学
·
药物递送系统
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产品关联
DOI
PubMed
ACS Omega
·
2021
·
IF 3.512
Zhifeng Hu,Haijuan Kong,Lei Tao,Mengmeng Qiao,Dongzi Yu,Feiyang Lu,Ahmed Dawelbeit,Muhuo Yu
The present paper provides a solution to enhance the reliability of bonding. The effect of the nonwoven carbon tissue (NWCT) composite adhesive layer on the bonding strength and reliability of aluminum alloy of single lap joints (SLJ) was investigated by embedding NWCT into the epoxy adhesive layer. The bonding strength, Weibull distribution, metallography of cross section, and fracture surface morphology of NWCT specimens were investigated. The results showed that the average bonding strength and Weibull characteristic strength (WCS) of NWCT-reinforced specimen were 16.78 and 17.17 MPa, which increased by 70.2 and 66.7%, respectively, compared with the neat specimen, and the Weibull modulus increased from 11.46 to 22.83, which indicated that NWCT specimens had higher bonding reliability. The mechanism of microcrack formation was obtained by analyzing the cross section of specimen loaded 95% WCS without macroscopic damage. The metallographic section showed that the microcrack of the neat specimen originated from the adhesive–aluminum interface, while the microcracks of the NWCT specimen originated from the interface between short carbon fibers (SCF) and adhesive. Typical failure modes were gained from visual observation and SEM. The failure mode of the neat specimen included more Al–adhesive interface failure, while the NWCT specimen included more internal failure of adhesive–SCFs with the fracture, pullout, peeling, and slippage of SCFs improving the toughness and bonding strength of the adhesive layer. The bridging effect of SCFs in the adhesive layer reinforced by NWCT can even the load and release the stress to improve the bonding reliability.
研究领域:
材料科学
·
工程学
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DOI
高影响因子
JOURNAL OF THERMOPLASTIC COMPOSITE MATERIALS
·
2026
·
IF 4
Zhenyang Qi,Jianxiang Lin,Shiqiang Yan,Kezhang Zhang,Wenhao Fan,Qiang Zhang,Shaoping Chen
Carbon fiber-reinforced polymer (CFRP) composites are increasingly employed in automotive, aerospace, and related industries to achieve lightweight structural designs. Although friction stir lap welding (FSLW) has been widely utilized for metal-thermoplastic composite joining, most existing studies have focused on static strength and fracture behavior, with limited attention to fatigue performance and dynamic damage evolution. In this work, high-aluminum-content particle-filled PA66 composites were manufactured using an electric-field-activated hot-press sintering process and subsequently joined to 6061-T6 via FSLW. The dissimilar joints consistently failed on the composite side under both static and cyclic loading, exhibiting a static tensile strength of 1935 N. As the composite material governed joint failure, fatigue characterization concentrated on the composite side. At 41.7% of the static strength, the composite achieved a fatigue life of 602,999 cycles. These results demonstrate that the proposed dissimilar FSLW joint possesses promising fatigue resistance, supporting its potential application in lightweight transportation structures. The results also provide a basis for future studies aimed at optimizing interfacial design and enhancing the long-term durability of metal-polymer hybrid joints.
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DOI
高影响因子
JOURNAL OF ADHESION SCIENCE AND TECHNOLOGY
·
2026
·
IF 3.4
Ying Yang,Kaiming Li,Guorong Cui,Xinbo Wang
A series of bismaleimide-based prepolymers via the Michael addition reaction of N,N’-4,4’-diphenylmethane-bismaleimide (BDM) and 4,4’-diaminodiphenyl ether (ODA) were prepared and used as adhesives for bonding CFRP/stainless steel, which present excellent performance. The manipulating mechanism of the molar ratio on the structure-performance relationship was revealed. Stronger interaction of macromolecular chains (i.e. hydrogen bond force) becomes increasingly obvious with increase of the molecular weight (molar ratio of BDM/ODA decreases), evidenced by FTIR peak shift of characteristic functional group in macromolecular chains. The optimal BDM/ODA molar ratio of bismaleimide-based prepolymers is 6:5, on which lap shear strength (LSS) reaches a maximum value of 20.59 MPa; though the thermal stability (T5d = 301 °C) and compressive strength (123.94 MPa) are not the best amongst these prepolymer adhesives, it really meets the requirement for high-temperature resistance as adhesive for bonding CFRP/stainless steel; it is the manipulating of molecular weight that achieves a balance between the cohesive strength and interfacial adhesion strength, which contributes to the highest LSS value (20.59 MPa) and testified by fracture morphology of the bonding interface. This is a facile and low-cost method for enhancing the lap shear strength between CFRP and metal substrate.
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产品关联
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高影响因子
危险品化学品经营许可证（带存储）