COLLOIDS AND SURFACES B-BIOINTERFACES
·
2022
·
IF 5.999
Yuhui Wang,Shilong Ma,Xuanyu Liu,Yan Wei,Haofeng Xu,Ziwei Liang,Yinchun Hu,Xiaojie Lian,Di Huang
Hepatocellular carcinoma (HCC) is one of the deadliest tumors in the world with a high rate of recurrence and metastasis. Therefore, the most pressing issue today is the development of new drugs, diagnostic and therapeutic approaches for effective cancer treatment. Cancer stem cells (CSCs) play a pivotal role in tumor recurrence, tumor resistance, and tumor metastasis, which provides a new perspective on the development of liver cancer. In the study, a high-temperature thermal breakdown approach was used to create composite magnetic nanocubes modified by polyethyleneimine (PEI) and hyaluronic acid (HA). The Fe 3 O 4 nanocubes can recognize HCC stem cells via receptor−ligand binding of HA and CD44 (HA receptor). While loading a small molecule LDN193189 inhibited the expression of stemness-related genes OCT4 and Nanog . More crucially, the Fe 3 O 4 nanocubes significantly suppressed HCC cell proliferation and migration by regulating the expression of epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin , and N-cadherin. Dual targeting using magnetic and receptor-mediated targeting improved the uptake of the drug delivery system. Our findings imply that the medication delivery method might be a potential therapeutic strategy for HCC.
研究领域:
癌症生物学
·
纳米技术
·
药学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Biomaterials Science & Engineering
·
2026
·
IF 6
Yanpei Zou,Yining Huang,Chenyu Wang,Tianlong Zhang,Haocheng Xu,Hailong Li,Yitong Xue,Xiaosheng Ma,Linli Li,Fan Zhang,Feizhou Lyu
Bone regeneration is a multifaceted process that requires the coordinated interactions among osteoblasts, endothelial cells, and even osteoclasts. Current biomaterials, however, rarely combine the capacity for multicellular modulation with ease of fabrication and high biocompatibility. Herein, a simple strategy of introducing tannic acid (TA) and MgFe layered double hydroxide (MgFe-LDH), as metal-organic frameworks (MOFs), into silk fibroin (SF) hydrogel is developed to construct a highly biocompatible, multi-functional hydrogel (SF/TA/MgFeLDH) system. The MOFs structure not only enhances the mechanical properties but also avoids rapid degradation of conventional SF/TA hydrogel, providing a good match to bone regeneration. More importantly, the SF/TA/MgFeLDH hydrogel system exhibits good promotion of osteogenic differentiation and angiogenesis while simultaneously suppressing the osteoclastic differentiation in vitro, due to the sustained release of bioactive Mg2+ and Fe3+ ions. Mechanistically, bulk RNA sequencing (RNA-seq) revealed that the hydrogel promotes osteogenesis by upregulating endogenous bone morphogenetic protein-2 (BMP2), which subsequently activates the downstream BMP2/SMAD/RUNX2 signaling pathway. Finally, in multiple bone defect animal models, the system exhibits superior vascularized bone regeneration ability compared to SF/TA controls. This study provides a promising biomaterial with potential clinical application for targeting bone regeneration.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
American Journal of Translational Research
·
2018
·
IF 3.061
Qian Tang,Minji Tong,Gang Zheng,Liyan Shen,Ping Shang,Haixiao Liu
The Masquelet’s induced membrane (IM) technique is widely used to treat large segmental bone defects due to its physical priority and biological function. However, the underlying molecular mechanism of the IM on bone formation remains unknown. In the present study, rat bone marrow-derived mesenchymal stem cells (BMSCs) were used as an in vitro model and bone morphogenetic protein 2 (BMP-2) was used as a positive control to evaluate the effects of the IM on the osteogenic differentiation of BMSCs. Although the IM group did not exhibit a significant increase in the expression of Runt-related transcription factor 2 (Runx2), Collagen I (Col I), osteocalcin (OCN) and alkaline phosphatase (ALP) relative to the BMP-2 administration, the IM was considerably effective compared with the untreated group. Mechanistically, we found that the IM activated the Smad and mitogen-activated protein kinase (MAPK) pathways, which was further confirmed by application of specific inhibitors of Smad1/5/8 (LDN-193189) and ERK1/2 (U0126). After the combined treatment of the IM and LDN-193189 as well as U0126, the IM-induced increase in Runx2, Col I, and OCN expression was significantly inhibited. These results suggest that IM promotes the osteogenic differentiation of rat BMSCs by activating the Smad1/5/8 and MAPK pathways.
研究领域:
骨科
·
干细胞生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Wangyu Bi,Shanglin Cai,Liping Zhou,Jinglei Zheng,Yanxin Cui,Yingxian Li,Shuting Liu,Hongyu Chen,Shuang Sha,Jiaxin Li,Qiansu Ma,Zhaoshi Bao,Cencan Xing,Hongwu Du,Lei Wang
3D culture of mesenchymal stem cells (MSCs) more effectively supports spheroid formation and sustained growth factor secretion, creating a microenvironment conducive to neural differentiation compared to traditional monolayer culture. However, challenges in long-term culture, including heterogeneity, central necrosis, and scalability, hinder its application in neural regeneration therapies. This study presents an automated microfluidic hydrogel-enhanced spheroid formation and maintenance array (M-HESMA) chip, combining a basement membrane-like hydrogel, microwell array, and dynamic culture system. The hydrogel, comprising a dual-network of Pluronic F-127-heparin (PF127-Hep) and crosslinked hyaluronic acid (XL-HA) with growth factors, supports spheroid formation and growth. The M-HESMA chip is validated using stem cells from human exfoliated deciduous teeth (SHEDs), forming uniform spheroids with minimal necrosis. The derived neurospheroids (MSNSs) exhibit enhanced neural differentiation and paracrine potential. Dissociated MSNSs (MSNS diss ) are applied to 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson's disease (PD) mice, improving motor function and reducing pathological symptoms compared to monolayer-cultured SHEDs treatment. MSNS diss -treated mice show increased brain-derived neurotrophic factor (BDNF) levels, phosphoinositide 3-kinase/protein kinase b (PI3K/Akt) pathway activation, and decreased neuronal apoptosis. The M-HESMA chip provides a scalable, efficient platform for spheroid generation, offering a promising approach for MSCs-based therapies targeting neurodegenerative diseases.
研究领域:
再生医学
·
干细胞研究
·
神经科学
·
生物工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）