FOOD CHEMISTRY
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2025
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IF 9.8
Shaojin Ma,Yongyu Li,Yankun Peng,Sen Nie,Xue Bai,Jincheng Zhang
An accurate yet rapid assessment of pungency and color values is pivotal for the commercial processing of pepper, underscoring the critical need for high-throughput detection techniques. This study investigates a rapid method for determining the pungency and color value of paprika via LED–induced fluorescence spectroscopy. A simultaneous detection instrument was developed with a proposed fluorescence correction method to reduce the impact of excitation light reflected by the sample. The corrected spectra could improve the performance of pungency prediction. The best pungency model achieved a R c 2 of 0.941, R cv 2 of 0.935, RMSEC of 6813.41 SHU and RMSECV of 6959.97 SHU. The best color value model achieved an R c 2 of 0.862, R cv 2 of 0.822 RMSEC of 1.233 and RMSECV of 1.402. This study offers a practical solution for simultaneous quality assessment suitable for industrial deployment, with the potential to extend this methodology to diverse cultivars.
研究领域:
食品科学
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分析化学
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光谱学
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农业工程
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DOI
PubMed
高影响因子
ELECTROPHORESIS
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2018
·Vol. 40
·期 3
·425-430
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IF 2.569
Yating Zhang,Yan Zhang,Luqi Zhu,Pingang He,Qingjiang Wang
DNA methylation is a significant epigenetic modification and the methods for the detection of DNA methyltransferase (MTase) activity are important due to aberrant methylation closely related to the occurrence of cancer. In this study, a simple and rapid microchip electrophoresis (ME) coupled with LED-induced fluorescence (LEDIF) method was presented for the detection of Dam MTase activity. This strategy was based on methylation-sensitive endonuclease DpnⅡ which could recognize the same specific site 5′-GATC-3′ with Dam MTase in double-stranded DNA (dsDNA). The adenines in the specific site could be methylated by Dam MTase, then the special site could not be digested by DpnⅡ. Both methylated dsDNA and unmethylated dsDNA could be analyzed by ME-LEDIF after stained by SYBR gold. The results showed the fluorescence intensities of methylated dsDNA were directly proportional to Dam MTase activities in the range of 0.5–20 U/mL with a detection limit of 0.12 U/mL. Furthermore, the method could successfully be applied to evaluation experiments of Dam MTase inhibitors. The results confirmed the ME-LEDIF method is a promising approach for inhibitors screening of DNA MTase and development of anticancer drugs
研究领域:
分子生物学
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分析化学
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医学
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DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2015
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IF 2.979
Xiupei Yang,Huanhuan Gao,Fan Qian,Chuan Zhao,Xiangjun Liao
An internal standard method has been developed for the simultaneous determination of anthracycline antibiotics, doxorubicin (DOX) and daunorubicin (DAN), in rabbit plasma using capillary electrophoresis (CE) with in-column double optical-fiber LED-induced fluorescence detection (CE-ICDOF-LED-FLD). Rhodamine B (RhB) was selected as an internal standard because its emission wavelength is similar to that of the anthracycline antibiotics. Parameters including buffer pH, buffer concentration, organic solvents and separation voltage have been investigated to explore the sensitivity and separation efficiency of DOX and DAN. The optimal electrophoretic separation conditions were a borate buffer (15 mM, pH 9.0) containing 50% acetonitrile (v/v), 10 s hydrodynamic injection at a height of 20 cm and a separation voltage of 15 kV. The developed CE-ICDOF-LED-FLD method provides limits of detection of 18 and 13 ng/mL for DOX and DAN in rabbit plasma samples, respectively. The recoveries ranging from 93.7 to 104.8% and the relative standard deviations at 1.1–1.7% were achieved for DOX and DAN in spiked rabbit plasma samples.
研究领域:
分析化学
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药理学
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生物医学工程
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2019
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IF 3.858
Xixue Chen,Feng Hong,Weilin Zhang,Dazhen Wu,Tianhua Li,Futao Hu,Ning Gan,Jianyuan Lin,Qiqin Wang
It is crucially important to rapidly, simultaneously, and sensitively determine trace amounts of heavy metal ions in complex samples. Herein, a stirring bar modified with two kinds of encoded hairpin DNA probes (H 0 and H 0 ′) was used in a multiplexed strategy allowing for specific extraction of Hg 2+ and Ag + coupled to microchip electrophoresis (MCE) separation and LED induced fluorescence (LIF) detection. The extraction step utilizes stir bars, which are functionalized with designed hairpin DNA probes (H 0 with T T and H 0 ′ with C C mismatches in stems). This allows the specific capture of Hg 2+ and Ag + through C Ag + C and T Hg 2+ T interactions. These complexes are then enzymatically degraded by the action of exonuclease III (Exo III). The ions released during this enzymatic reaction can initiate a new cycle of interactions with hairpin structures and enzymatic reactions and so on. This cyclic step is specific to the presence of Hg 2+ and Ag + and represents the first round of amplification of the presence of the selected ions. The resulting single strand DNAs on the stirring bars after enzymatic degradation were used in the second step as primers to trigger the catalytic hairpin assembly (CHA) in the presence of a couple of hairpin structures in solution. Such a reaction allows producing duplexes that can be monitored by MCE-LIF. The fluorescence intensity of CHA products (I P ) increased and that of hairpin DNAs (I R ) decreased with the increase of target concentrations. The signal ratios (I P /I R and I P ′/I R ′) consisted of targets. The assay was employed for Hg 2+ and Ag + detection in several mediums including water, milk, and fish samples with complex matrices. The results showed that the assay could avoid matrix interference to increase the sensitivity. Therefore, the multiplexed assay was ideal to simultaneously and quickly detect metal ions in complex samples.
研究领域:
分析化学
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环境科学
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生物技术
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）