ENVIRONMENTAL TECHNOLOGY
·
2021
·
IF 3.247
Yangyang Wang,Wenhao Zhan,Yidan Liu,Shanshan Cheng,Chaosheng Zhang,Jianhua Ma,Runhua Chen
Di-n-octyl phthalate (DOP), a plasticizer used in many different industrial products, is a frequently observed pollutant in the environment. Biodegradation by microorganisms is considered to be a realistic choice for the remediation of DOP contamination. In the present research, the halotolerant bacterial consortium (LF) enriched in our previous research was used to degrade DOP. It was found that the optimal conditions for LF to degrade DOP was temperature 30oC, pH 6.0, inoculum size >5%, and salt content <3%. LF could degrade a high concentration of DOP (2000 mg/L) with the removal efficiency of 96.33%. Substrate inhibition analyses indicated that the inhibition constant, maximum specific degradation rate and half-saturation constant were 2544.6 mg/L, 0.7 d−1 and 59.1 mg/L, respectively. Based on the analysis of the gas chromatography-mass spectrometry (GC-MS), the biodegradation pathway for DOP by LF was proposed. Furthermore, LF could degrade DOP in soil (100 mg/kg) with the highest removal efficiency of 89.3%. This study is the first report on DOP biodegradation by bacterial consortium. These results suggest that LF can be used to remediate DOP-contaminated environment.
研究领域:
环境科学
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微生物学
·
生物技术
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yaling Zhang,Shuai Chao,Xi Zhang,Junguo Li,Yiming Duan,Chunhui Gu,Jiale He
The iron and steel industry is facing two urgent needs of comprehensive utilization of bulk solid waste and CO 2 emission reduction. Using LF refining slag as raw material to prepare efficient calcium-based CO 2 adsorbents can achieve the goals of waste-to-resource conversion and cleaner production. In this study, LF refining slag-derived CaO/C 12 A 7 high-temperature calcium-based CO 2 adsorbent materials with abundant pore defects were synthesized by the co-precipitation method, which showed the optimal high-temperature adsorption performance at 700 °C and pure CO 2 atmosphere, with an adsorption capacity of up to 0.681 g/g, which is 1.58 times that of the adsorption capacity of commercial CaO. C 12 A 7 , as an inert skeleton of adsorbent material, can inhibiting the agglomeration of CaO particles and slowing down the sintering deactivation process. After 20 cycles of calcination-carbonation, the adsorption capacity degradation rate was 32.5 %, lower than that of commercial CaO at 37.8 %. The related research effectively enhances the added value of resource utilization from LF refining slag and holds the potential to achieve a balance among environmental, economic, and social benefits in the steel industry.
研究领域:
材料科学
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环境科学
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冶金工程
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DOI
高影响因子
Food & Function
·
2020
·Vol. 11
·期 4
·3235-3244
·
IF 4.171
Yujin Liu,Minhao Xie,Peng Wan,Guijie Chen,Chunxu Chen,Dan Chen,Shijie Yu,Xiaoxiong Zeng,Yi Sun
Dicaffeoylquinic acids (DiCQAs), the main components of kudingcha made from the leaves of Ilex kudingcha, could be transformed by gut microbiota. However, the information about the related microorganisms and enzymes involved in the biotransformation of DiCQAs in the human gut is limited. Therefore, a strain of bacteria that could hydrolyze DiCQAs, belonging to Lactobacillus fermentum named L. fermentum LF-12, was isolated from human feces in the present study. Furthermore, an esterase for the hydrolysis of DiCQAs was purified from L. fermentum LF-12 and heterogeneously expressed in Escherichia coli. The esterase could be induced to exert superior hydrolytic activity in the presence of lactose as the carbon source. The molecular weight of the purified esterase was determined to be 31.9 kDa, and the isoelectric point, optimal pH and temperature for the esterase were 4.71, 6.5 and 45 °C, respectively. The enzyme activity was improved by Mg2+ and Ca2+, and reduced by Co2+, Cu2+, EDTA and some kinds of organic solvents. The present results provide new insights into the metabolism of DiCQAs by the human gut.
研究领域:
食品科学
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微生物学
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生物化学
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产品关联
DOI
PubMed
高影响因子
PLoS One
·
2018
·Vol. 13
·期 10
·e0204324
·
IF 2.766
Fangfang Li,Yidan Liu,Diwei Wang,Chaosheng Zhang,Zhihui Yang,Siqi Lu,Yangyang Wang
A halotolerant bacterial consortium capable of degrading di-(2-ethylhexyl) phthalate (DEHP) was enriched from activated sludge. Community analysis revealed that LF contained seven families and seven genera of bacteria. The predominant species was Gordonia sp. (54.93%), Rhodococcus . sp. (9.92%) and Achromobacter sp. (8.47%). The consortium could degrade 93.84% of 1000 mg/l DEHP after 48 h incubation. The optimal temperature and pH for LF to degrade DEHP were 30 °C and 6.0, respectively. LF degraded more than 91% of DEHP with salt concentrations ranging from 0–3%. The inoculum size had great effects on DEHP degradation (incubation time < 24h). LF could degrade high concentrations of DEHP (from 100 to 2000 mg/l) with the degradation ratio above 92% after 72 h incubation. Kinetics analysis revealed that the degradation of DEHP by LF was best fitted by the first-order kinetics when the initial concentration ranged from 100 to 2000 mg/l. The main intermediates (2-ethylhexyl pentyl phthalate, butyl (2-ethylhexyl) phthalate (BEHP), mono-ethylhexyl phthalate (MEHP), mono-hexyl phthalate (MHP), mono-butyl phthalate (MBP)) in DEHP degradation process were identified using gas chromatography–mass spectrometry (GC-MS), and a new complex biochemical pathway was proposed. Furthermore, LF could also degrade dimethyl phthalate (DMP), diethyl phthalate (DEP), di- n -butyl phthalate (DBP), di- n -octyl phthalate (DOP) and phthalic acid (PA).
研究领域:
环境科学
·
微生物学
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DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2020
·
IF 3.99
Chengyong Gao,Xueni Hou,Tieling Xing,Guoqiang Chen
The water retention of printing inks is of the utmost importance. According to low -field nuclear magnetic resonance (LF-NMR) to detect the behavior of polyols and water, polyols with excellent water retention performance can be selected quickly. The water retention of disperse ink is closely related to the intramolecular/intermolecular forces of each component in the disperse ink. The relationship between each component of the ink is revealed by LF-NMR. The water retention of ink was also evaluated by LF-NMR. Therefore, the LF-NMR was able to design ink formulation and evaluate ink quality. Compared with NNO and TD-1109, Surfynol CT-136 had a higher grinding efficiency for disperse dyes. The physicochemical properties of disperse ink was evaluated to meet the requirements of inkjet printing ink. The rheological properties of the disperse inks were characterized by rotary rheometer, which were confirmed as Newtonian fluid ; The quality of the ink-jet printing pattern is closely related to the ink jet performance. The formation process of the ink droplet and the parameters of the ink droplets were observed by the ink droplet observer. The ink has excellent jetting properties and a high-quality print pattern at an appropriate viscosity (3.1 mPa.s) and surface tension (34.2 mN/m). The excellent affinity between NVP/VA copolymer (NVP/VA64) and disperse dyes was confirmed by ultraviolet spectrophotometer (UV spectrophotometer). The scanning electron microscope (SEM) images of printed fabrics show that there is a film on the surface. The NVP/VA copolymer can be used as a carrier for disperse dye to improve the fixation and fastness of disperse dye in polyester fabrics.
研究领域:
材料科学
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化学工程
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2024
·
IF 5.3
Ariana Sheng-Chu Leon,Kenneth C. Waterman,Guanhua Wang,Likun Wang,Ting Cai,Xiaohua Zhang
Amorphous solid dispersions (ASDs) represent an important approach for enhancing oral bioavailability for poorly water soluble compounds; however, assuring that these ASDs do not recrystallize to a significant extent during storage can be time-consuming. Therefore, various efforts have been undertaken to predict ASD crystallization levels with kinetic models. However, only limited success has been achieved due to limits on crystal content quantification methods and the complexity of crystallization kinetics . To increase the prediction accuracy, the accelerated stability assessment program (ASAP), employing isoconversion (time to hit a specification limit) and a modified Arrhenius approach, are employed here for predictive shelf-life modeling. In the current study, a model ASD was prepared by spray drying griseofulvin and HPMC-AS-LF. This ASD was stressed under a designed combinations of temperature, relative humidity and time with the conditions set to ensure stressing was carried out below the glass transition temperature (T g ) of the ASD. Crystal content quantification method by X-ray powder diffraction (XRPD) with sufficient sensitivity was developed and employed for stressed ASD. Crystallization modeling of the griseofulvin ASD using ASAP prime ® demonstrated good agreement with long-term (40 °C/75 %RH) crystallinity levels and support the use of this type of accelerated stability studies for further improving ASD shelf-life prediction accuracy.
研究领域:
药剂学
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药物化学
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产品关联
DOI
PubMed
高影响因子
GREEN CHEMISTRY
·
2015
·Vol. 17
·期 6
·3644-3652
·
IF 8.02
Shanshan Li,Ning Li,Guangyi Li,Lin Li,Aiqin Wang,Yu Cong,Xiaodong Wang,Tao Zhang
Protonated phenol-formaldehyde condensation products of sodium lignosulfonate and lignocellulose derived aldehydes were first reported as cost-effective catalysts for the hydroxyalkylation/alkylation (HAA) of 2-methylfuran and furfural. Among them, the LF resin which was prepared with sodium lignosulfonate and formaldehyde exhibited the best performance. With the LF resin, higher activity, catalytic efficiency and stability than those of Amberlyst resins were observed, which can be rationalized by its higher acidic strength. The HAA product of 2-methylfuran and furfural was further converted to diesel and jet fuel range alkanes by the solvent-free hydrodeoxygenation (HDO) over the Ni/H-ZSM-5 catalysts prepared by conventional or complexation impregnation methods. It was found that the presence of complex agents can promote the dispersion of Ni, which leads to the higher HDO activity of Ni/H-ZSM-5 catalysts.
研究领域:
化学工程
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催化材料
·
能源科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 11
·9325
·
IF 5.6
Meng Ji,Liwei Ren,Chunyuan Tian,Xuming Zhuang,Feng Luan
In this paper, glutathione (GSH)-coated Zn-doped CdTe quantum dots (QDs) with different particle sizes were synthesized using the “reflow method”, and the interaction mechanism between the two QDs and lactoferrin (LF) was investigated systemically with different spectroscopic methods. The steady-state fluorescence spectra showed that the LF formed a tight complex with the two QDs through static bursting and that the electrostatic force was the main driving force between the two LF–QDs systems. The complex generation process was found to be spontaneous (ΔG < 0) and accompanied by exothermic and increasing degrees of freedom (ΔH < 0, ΔS > 0) by using the temperature-dependent fluorescence spectroscopy. The critical transfer distance (R0) and donor–acceptor distance (r) of the two LF–QDs systems were obtained based on the fluorescence resonance energy transfer theory. In addition, it was observed that the QDs changed the secondary and tertiary structures of LF, leading to an increase in the hydrophobicity of LF. Further, the nano-effect of orange QDs on LF is much larger than that of green QDs. The above results provide a basis for metal-doped QDs with LF in safe nano-bio applications.Keywords:quantum dots (QDs);lactoferrin (LF);interaction;spectroscopic methods;thermodynamic constants;nano-effects
研究领域:
生物化学
·
纳米生物学
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产品关联
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2016
·
IF 3.42
Yatao Yin,Chaoping Fu,Mei Li,Xiangpen Li,Mengying Wang,Lei He,Li-Ming Zhang,Ying Peng
Gliomas are the most common and lethal type of primary malignant brain tumor. But the existence of blood brain barrier (BBB) and blood-tumor barrier (BTB) hinder drug from reaching the tumor site. To address this problem, we developed a novel prodrug (Lf-HA-DOX) by conjugating hyaluronic acid with doxorubicin (HA-DOX) by an acid-labile hydrazone linkage, which is released in an acidic environment and accumulates in tumor tissues. Lactoferrin (Lf) was coupled for transporting across the BBB. In vitro, the release of DOX from Lf-HA-DOX was pH-dependent. At lower pH (5.0 and 6.0), the release of DOX was much quicker than that at pH 7.4. In the cellular uptake studies, flow cytometry analyses and confocal laser scanning microscopy results showed significantly enhanced cellular uptake of Lf-HA-DOX and HA-DOX in C6 cells compared to DOX. In BALB/C mice bearing C6 glioma, enhanced accumulation of Lf-HA-DOX in the glioma was observed by real time fluorescence image. Correspondingly, glioma-bearing mice treated with Lf-HA-DOX displayed the longest median survival time, which was 2-fold longer than that of saline group. In conclusion, Lf-HA-DOX was able to significantly increase drug delivery to the glioma, which might provide a promising strategy for antiglioma therapy.
研究领域:
药理学
·
神经科学
·
肿瘤学
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
CEMENT AND CONCRETE RESEARCH
·
2025
·
IF 13.1
Jing Qiao,Chunsheng Zhou,Jingjing Feng,Miao Miao,Yanliang Ji,Linan Gu
Limestone powder (LS) with large particle sizes is widely used, but its effect on water migration behavior remains unclear. This study investigates the influence of LS and viscosity-enhancing admixtures (VEAs) on the water migration behavior and water distribution in fresh cement paste using 1 H low-field nuclear magnetic resonance (LF-NMR). Results indicate that LS increases the size of water migration channels, accelerates the water migration rate, and raises the bleeding ratio, while VEAs substantially mitigate the effects of LS. Compared to polyacrylamide, cellulose ether significantly slows down the water migration rate before 40 min, thereby reducing the bleeding ratio. Additionally, relaxation times were found to decrease during bleeding and can reflect the sedimentation process. It is confirmed that the pore structure of fresh paste exhibits fractal characteristics, with the fractal dimension closely correlating with water migration rates. The underlying mechanisms and their implications on material behavior are thoroughly analyzed and discussed.
研究领域:
材料科学
·
土木工程
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Gongduan Fan,Chenjian Cai,Zhuoyi Chen,Jing Luo,Banghao Du,Shangwu Yang,Jiaxin Wu
The poor light transmittance , low recycling rate and potential secondary pollution problems of powder photocatalysts limit the practical application of photocatalytic technology. Therefore, there is an urgent need to develop stable floating photocatalysts to alleviate the problem of harmful algal blooms (HABs). In this work, a floating Ag 2 MoO 4 /TACN@LF composite was synthesized for the inactivation of Microcystis aeruginosa ( M. aeruginosa ) by employing a simple oscillatory impregnation method. The Ag 2 MoO 4 /TACN@LF floating photocatalyst exhibited stable algae removal performance under various conditions, and 100% chlorophyll a removal rate could be achieved within 4 h of visible light irradiation under the optimal photocatalyst loading of 6 g/L. The effects of Ag 2 MoO 4 /TACN@LF floating catalyst on algal cell membrane permeability, phycobiliprotein, intracellular antioxidant enzyme activities and algal organic matter were further studied. Moreover, the scavenging experiments demonstrated that •OH was the predominant reactive oxygen species (ROSs). Ag0 and 3 g-C 3 N 4 * also made a non-negligible contribution to the inactivation of algal cells. What's more, the Ag 2 MoO 4 /TACN@LF still exhibited 92% inactivation efficiency for M. aeruginosa after 5 cycles reused experiments, indicating its promising potential for practical application of alleviating HABs.
研究领域:
环境科学
·
微生物学
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DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Dan Peng,Qi Zhou,Min Su,Shaoshuai Zheng,Shuli Xie,Jun Li
Low-field nuclear magnetic resonance (LF-NMR) combined with chemometrics was used for the rapidly and nondestructively quantitative determination of carbonyl value (CV) of frying oils during frying process. The quality indices of frying oils during frying process were analyzed as well as the changes of its LF-NMR signal, followed by exploring the correlation between the quality indices of frying oils and the relaxation-characteristic indices and investigating the influences of modeling data and preprocessing methods on the modeling performance. Based on the echo decay curves data of LF-NMR and the relaxation-characteristic indices data, the prediction model of CV was established by partial least square (PLS), principal component regression (PCR), multiple linear regression (MLR), and support vector machine regression (SVR). Results showed that there was a significant correlation between CV and relaxation-characteristic indices ( p < 0.01) with both modeling data and preprocessing methods affecting the model performance. The prediction performance of non-linear SVR model was significantly superior to that of linear modeling methods (PLS, PCR, and MLR). The best prediction model of CV was built using the SVR method based on echo decay curves data. The root mean square error of prediction and coefficient of determination were 2.599 mmol/kg and 0.9873, respectively.
研究领域:
食品科学
·
分析化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）