Inhibition of fish rhabdovirus demonstrates application prospect of two methylimidazole phenylpropanoid-based small molecules in aquaculture
Aquaculture faces significant challenges, notably spring viremia of carp (SVC) caused by spring viremia of carp virus (SVCV), which is particularly devastating due to its high mortality rates and rapid transmission among fish populations. This study introduces two novel methylimidazole phenylpropanoid-based small molecules, LML1013 and LML1014, synthesized for combating SVCV in aquaculture environments. Comprehensive in vitro and in vivo evaluations demonstrated that these compounds have potent antiviral activities against SVCV. They effectively inhibited SVCV replication in epithelioma papulosum cyprinid (EPC) cells and significantly reduced mortality rates in infected juvenile common carp. Further analyses revealed that daily administration of these compounds not only blocked SVCV replication but also stimulated the innate immune responses of the fish, thereby enhancing their overall resistance to viral infection. Notably, the antiviral efficacy was observed in a dose- and time-dependent manner without inducing cytotoxic effects, emphasizing their therapeutic potential. The stability of LML1013 and LML1014 in aquacultural water and their ability to prevent horizontal viral transmission were also confirmed, further supporting their feasibility as treatment options in aquaculture. Overall, the development of LML1013 and LML1014 provides a promising strategy for controlling SVCV outbreaks and enhancing the sustainability of fish farming operations. This approach offers significant implications for improving aquaculture biosecurity and effectively managing viral disease threats.
危险品化学品经营许可证（带存储）