MICROCHIMICA ACTA
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2019
·Vol. 186
·期 12
·1-8
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IF 5.479
Yang Guotai,Huang Min,Wang Yutong,Chen Guanhua,Zhao Yu,Xu Hengyi
A lectin magnetic separation (LMS) method for Staphylococcus aureus ( S. aureus ) was developed with the aim to improve the efficiency of magnetic nanoparticles and to expand the scope of bacterial recognition. Poly(ethylene glycol) (PEG)-mediated magnetic nanoparticles modified with streptavidin (MNP-PEG-SA) were synthesized and then applied to a two-step LMS based on the use of wheat germ agglutinin (WGA). Three specific methods for S. aureus detection (suitable for different requirements including detection time and sensitivity) were designed. The new LMS has improved anchoring efficiency (compared to two-step LMS methods) and requires a reduced number of magnetic particles. The Baird–Parker (B-P) method can detect S. aureus with a detection limit of 3 × 10 0 CFU·mL −1 within 15 h; the polymerase chain reaction (PCR) method can be finished within 4 h, with the lowest detection limit (LOD) of 3 × 10 2 CFU·mL −1 . The LOD of HRP-pig IgG-based colorimetric method is 3 × 10 5 CFU·mL −1 , and the method only lasts for 2 h. If combined with specific detection methods, it meets different needs for rapid detection of S. aureus .
Schematic representation of lectin magnetic separation (LMS) based on biotin-wheat germ agglutinin (WGA) and poly (ethylene glycol) (PEG)-mediated streptavidin-modified magnetic nanoparticles (MNP-PEG-SA) and three different quantification strategies (including B-P culture assay, PCR assay, and colorimetric assay) for S. aureus .
研究领域:
微生物学
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纳米技术
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分析化学
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分子生物学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Guotai Yang,Xiangyu Meng,Yutong Wang,Mingyao Yan,Zoraida P. Aguilar,Hengyi Xu
A novel 2-step lectin-magnetic separation (LMS) method combined with gold nanoparticle (AuNPs) -based colorimetric system for specifically and quantitatively detecting S. aureus in blood samples was established. In this study, wheat germ agglutinin lectin from Triticum vulgaris (wheat) (WGA) was applied for the first time as a recognition agent in a 2-step magnetic separation method using magnetic nanoparticles (MNPs) for effective and low-cost bacteria enrichment. A quantitative detection strategy based on the long dsDNA amplicons stabilized with AuNPs against salt-induced aggregation was investigated. The 2-step LMS method resulted in a high enrichment efficiency even at low concentrations (10 0 –10 2 CFU/mL) of S. aureus in pure culture and blood samples. The AuNPs-based colorimetric system was applied for quantitative detection of S. aureus with a limit of detection (LOD) at 3.5 × 10 0 CFU/mL. The complete process starting with the blood samples treatment, 2-step LMS, and AuNPs-based colorimetric detection took only 7 h. The low cost and sensitive 2-step LMS combined with AuNPs-based colorimetric detection holds considerable potential for early sepsis diagnosis.
研究领域:
生物技术
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纳米技术
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微生物学
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临床诊断
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DOI
高影响因子
Scientific Reports
·
2021
·Vol. 11
·期 1
·1-11
·
IF 4.38
Yang Zuoyi,Mao Xingchen,Cui Jiahao,Wang Yujie,Zhang Yaping
This study examines the specific effect of Tween 80 on the conversion of anthracene (ANT) in laccase medium system regarding surfactant chemical changes and mechanism. The conversion rate and degradation products of ANT were investigated in different concentrations of Tween 80 solution. Between Tween 80 concentration 0–40 critical micelle concentrations (CMC), the kinetic parameter-k (h −1 ) and corresponding half-life T 1/2 decreased with increasing concentration. When Tween 80 was above 20 CMC the laccase-medium system converted > 95% of ANT to anthraquinone within 12 h. During the entire enzymatic reaction, the laccase activity in the system increased with increasing Tween 80 concentration. Combined with GC/MS analysis of the product, it was speculated that hydrogens belonging to the ether-oxygen bond and carbon–carbon double bond α-CH of Tween 80, were removed by the laccase-media system, promoting its degradation. Additionally, enhanced activity caused by oxygen free radicals (ROS) such as RO• and ROO•, continuously oxidized Tween 80, which in turn produced free radicals while converting ANT. This study provides new theoretical support toward the application of surfactants in the elimination of polycyclic aromatic hydrocarbons.
研究领域:
环境科学
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生物化学
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DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2025
·Vol. 36
·期 3
·1-7
·
IF 2.8
Luo Zhenli,Cai Yiyang,Yin Changzhi,Cheng Mingfei,Lei Weicheng,Lu Wenzhong,Song Xiaoqiang,Lei Wen
A novel BaMgSi 4 O 10 ceramic was fabricated using a conventional solid-state method. The BaMgSi 4 O 10 accompanied with BaMgSiO 5 , Ba 2 MgSi 2 O 7 , and SiO 2 was detected for all compositions. Furthermore, microwave dielectric properties with ε r = 5.7 , Q × f = 17,900 GHz, and τ f = − 19.1 ppm/°C were obtained at 1100 °C. To meet the requirement of LTCC technology, the optimum temperature can be lower than 850 °C by composing with 2 wt% LMS glass with a short soaking time of 0.5 h. A patch antenna was fabricated using BaMgSi 4 O 10 -2 wt% LMS as the substrate with a bandwidth of 120 MHz at 4.12 GHz. All result indicates BaMgSi 4 O 10 -2 wt% LMS ceramic has large application prospect in wireless communication systems.
研究领域:
材料科学
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电子工程
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DOI
高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-7
·
IF 5.228
Jin Xiaoting,Yu Xiangyang,Zhu Guangyan,Zheng Zuntao,Feng Fayun,Zhang Zhiyong
A high capacity of laccase from Trametes versicolor capable of degrading pesticides has been revealed. The conditions for degrading of five selected pesticides including chlorpyrifos, chlorothalonil, pyrimethanil, atrazine and isoproturon with the purified laccases from Trametes versicolor were optimized. The results showed that the optimum conditions for the highest activity were pH at 5.0 and temperature at 25 °C. The best mediators were violuric acid for pyrimethanil and isoproturon, vanillin for chlorpyrifos, and acetosyringone and HBT for chlorothalonil and atrazine, respectively. The laccase was found to be stable at a pH range from 5.0 to 7.0 and temperature from 25 to 30 °C. It was observed that each pesticide required a different laccase mediator concentration typically between 4.0–6.0 mmol/L. In the experiment, the degradation rates of pyrimethanil and isoproturon were significantly faster than those of chlorpyrifos, chlorothalonil and atrazine. For example, it was observed that pyrimethanil and isoproturon degraded up to nearly 100% after 24 hours while the other three pesticides just reached up 90% of degradation after 8 days of incubation.
研究领域:
环境生物学
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酶学
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DOI
PubMed
高影响因子
Inorganic Chemistry Frontiers
·
2025
·
IF 6.4
Yuhui Weng,Xingyu Sun,Siying Li,Zhuoying Wu,Ziwei Chen,Yongqun Ma,Zefan Zheng,Feng Lin,Ming Zhang,Kejun Zhang,Min Ling,Keli Yang,Chengdu Liang,Jun Chen
High-capacity silicon (Si)-based anodes have been recognized as particularly promising candidates for high-energy-density lithium-ion batteries (LIBs). Rational design and tailoring of an artificial interface for Si anodes can effectively mitigate volume expansion, suppress detrimental side reactions, and enhance lithium-ion (Li+) diffusion kinetics—three critical factors widely acknowledged as fundamental prerequisites for enabling long-term cycling stability and fast-charging capabilities. Therefore, we introduced an organic/inorganic composite interface design strategy combining high elasticity and ion-conductive properties. Lithium metasilicate (LMS) not only functions as an effective protective layer and Li+ conductor but also inhibits lithium hexafluorophosphate (LiPF6) hydrolysis to suppress the generation of corrosive hydrofluoric acid (HF). When integrated with carboxyl-rich polyacrylic acid (PAA), the nanocomposite coating layer demonstrates high elasticity to accommodate volume expansion, establishes continuous Li+ diffusion pathways, and promotes the formation of a robust lithium fluoride (LiF)-rich artificial solid-electrolyte interphase (SEI). Consequently, the as-developed Si@PL-10 electrode demonstrates significantly enhanced cycling performance (2297 mAh g−1 after 200 cycles at 1 A g−1) and high-rate capability (1854 mAh g−1 at 6 A g−1). This work provides valuable insights for designing scalable multifunctional coatings for high-performance Si anodes.
研究领域:
储能技术
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材料科学
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电化学
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纳米技术
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电池技术
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DOI
高影响因子
危险品化学品经营许可证（带存储）