Buckminster fullerene (C 60 ), the most representative type among fullerenes, has attracted widely attentions because of its many potential applications. The increasing application of fullerene and limited knowledge of its environmental fate are required concerns. Herein, the biotransformation of C 60 by Labrys sp. WJW was investigated. Cell numbers reached 25.76 ± 1.85 folds within 8 days using 100 mg/L C 60 as sole carbon source. The biotransformation of C 60 by Labrys sp. WJW was analyzed by various characterization methods. Raman spectra indicated that strain WJW broke the soccer ball like structure of C 60 . After 12 days, over 60% of C 60 was degraded evidenced by UV–vis spectrophotometry and liquid chromatography-mass spectrometry. The underlying biotransformation mechanism of C 60 through an extracellular Fenton-like reaction was illustrated. In this reaction, the • OH production was mediated by reduction of H 2 O 2 involving a continuous cycle of Fe(II)/Fe(III). Bacterial transformation of C 60 will provide new insights into the understanding of C 60 bioremediation process.
Carbon nanotubes (CNTs) have been widely studied because of their potential applications. The increasing applications of CNTs and less known of their environmental fates rise concerns about their safety. In this study, the biotransformation of multi-walled carbon nanotubes (MWCNTs) by Labrys sp. WJW was investigated. Within 16 days, qPCR analysis showed that cell numbers increased 4.92 ± 0.36 folds using 100 mg/L MWCNTs as the sole carbon source. The biotransformation of MWCNTs, which led to morphology and functional group change, was evidenced by transmission electron microscopy and X-ray photoelectron spectroscopy analyses. Raman spectra illustrated that more defects and disordered carbon appeared on MWCNTs during incubation. The underlying biotransformation mechanism of MWCNTs through an extracellular bacterial Fenton-like reaction was demonstrated. In this bacteria-mediated reaction, the OH production was induced by reduction of H 2 O 2 involved a continuous cycle of Fe(II)/Fe(III). Bacterial biotransformation of MWCNTs will provide new insights into the understanding of CNTs bioremediation processes.
危险品化学品经营许可证（带存储）