Probiotics and Antimicrobial Proteins
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2024
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IF 4.4
Feng Xiaoyan,Wang Mengqi,Wen Siyue,Hu Liehai,Lan Yuzhi,Xu Hengyi
Mitigating steatosis is essential for delaying the progression of alcoholic liver disease. The effect and mechanism of Lactiplantibacillus plantarum P101 (LP.P101) on alleviating alcohol-induced hepatic lipid accumulation were investigated in our study. The mouse model was constructed by a short-term (10-day)-plus-binge ethanol feeding and gavaged with 10 8 CFU/mL of LP.P101 daily. Lipid droplet in the liver was significantly reduced by LP.101 intervention on AMPK activation. However, when AMPK was inhibited by dorsomorphin, the levels of related indicators (ALT, TG, etc.) and the expression levels of AMPK and relevant genes in the liver converged to that of the alcohol-fed group. Compared with the alcohol-fed group, LP.P101 reduced the relative abundance of Firmicutes and increased that of Bacteroidetes. Parabacteroides merdae was negatively correlated with lipid accumulation, and unclassified Negativibacillus was negatively associated with AMPK activation. Importantly, LP.P101 modified the compositions of the serum metabolites. The potential biomarker stercobilinogen was positively correlated with AMPK activation and negatively associated with lipid accumulation. This work confirmed that LP.P101 attenuated alcohol-induced hepatic lipid accumulation in mice through AMPK activation, and the alterations in gut microbiota and metabolites may play a significant role on AMPK activation.
研究领域:
肝病学
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微生物学
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代谢组学
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分子生物学
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DOI
PubMed
高影响因子
International Journal of Nanomedicine
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2024
·
IF 6.6
Lixin Xie,Lihua Feng,Xiaomin Tang,Yunping Xu,Hengyi Xu,Yang Liu
Purpose To evaluate the therapeutic effect of oral administration of Lactiplantibacillus plantarum P101 (P101) on skeletal injury in young rats exposed to titanium dioxide nanoparticles (TiO2 NPs), and explore the potential mechanism.Methods Four-week-old male rats were orally administration to TiO2 NPs and supplemented with P101 2 hours later for 4 weeks. The growth and development, food intake, bone metabolism and serum inflammatory markers of the rats were evaluated. Their tibias were observed and evaluated using microcomputed tomography (micro-CT), tartrate-resistant acid phosphatase (TRAP) staining, immunohistochemistry (IHC) and real-time quantitative PCR (RT-qPCR). We observed the tibia growth plate using safranin and fast green staining. 16S rDNA sequence analysis of fecal samples was performed to observe changes in the gut microbiota.Results Our results showed that TiO2 NPs can lead to bone growth inhibition and osteoporosis, induce intestinal flora imbalance, and induce inflammation in young rats. Further mechanistic studies suggested that TiO2 NPs disrupts intestinal flora and increases serum IL-1β levels, which increased the expression of RANKL in bone, thereby enhancing osteoclast differentiation and function, leading to bone loss. Through a P101 supplementation experiment, we found that P101 ameliorated the inflammation and osteoporosis on bone caused by TiO2 NPs.Conclusion This study showed that the mechanism by which P101 alleviates bone damage caused by TiO2 NPs may be through restoring intestinal microbial homeostasis and inhibiting inflammatory response.
研究领域:
微生物学
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免疫学
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骨骼生物学
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纳米技术
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药理学
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DOI
PubMed
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
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2024
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IF 3.9
Penghui Nie,Siyue Wen,Mengqi Wang,Hengyi Xu
The decline in male sperm quality caused by multiple factors has become a widespread concern. Alcohol excessive consumption is one of the factors that induce testicular dysfunction. Testicular dysfunction caused by alcohol abuse is related to oxidative stress and inflammation. Probiotics can ameliorate alcohol-induced testicular dysfunction. However, the specific mechanism is not explicit. This study aimed to elucidate the underlying mechanism by which Lactiplantibacillus plantarum P101 ameliorates the alcohol-induced testicular dysfunction. The model of alcohol-induced testicular dysfunction in C57B/6 male mice was established according to the National Institute on Alcohol Abuse and Alcoholism, and Lactiplantibacillus plantarum P101 supplementation was orally administered to mice during the experiment. The results showed that Lactiplantibacillus plantarum P101 promoted androgen production, reduced testis inflammation, and improved testis antioxidant capacity , thereby improving sperm quality and sperm motility and ultimately ameliorating alcohol-induced testicular disorder. Three key metabolite pathways and six key metabolites were identified by metabolome analysis.
研究领域:
微生物学
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生殖生物学
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代谢组学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
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2026
·Vol. 91
·期 3
·e71000
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IF 4.1
Xiaoyan Feng,Xiaowei Xu,Jinfeng Zhang,Hengyi Xu
Surging Luffa sponge demand yields high by-product wastage, impacting resource efficiency. Noted for boosting resource efficiency, lactic acid bacteria fermentation is widely used in agricultural by-product processing. In this study, we used Lactiplantibacillus plantarum P101 (LpP101) to ferment mature Luffa to prepare the Luffa fermentation broth (LF). Moreover, we found that LpP101 fermentation changed the physicochemical properties and chemical compositions of the Luffa solution and improved its potential function. Furthermore, in vivo experimental results showed that LF performed superior protective effect against cyclophosphamide (CTX)-induced hepatotoxicity than the unfermented Luffa juice (LJ). Therefore, the LpP101 fermentation improved the resource efficiency and functional characteristics of Luffa . Practical Applications This study fermented the mature Luffa using LpP101 to obtain the LF. The LF showed an excellent antioxidant ability, and included various bioactive components than the LJ. Moreover, the LF showed a better protective effect against CTX-induced hepatotoxicity than the LJ. Our study aims to seek the comprehensive utilization method for Luffa by-products, and further improve their nutritional contents. It offers a way to boost the economic and additional value of Luffa by-products, increasing the wide application of mature Luffa .
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高影响因子
危险品化学品经营许可证（带存储）