BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2016
·Vol. 39
·期 4
·603-611
·
IF 1.901
Zhang Jian-Dong,Cui Zhi-Mei,Fan Xiao-Jun,Wu Hua-Lei,Chang Hong-Hong
Two uncharacterized nicotinamide adenine dinucleotide (NADH) oxidases (named as LpNox1, LpNox2) from Lactobacillus pentosus ATCC 8041 were cloned and overexpressed in Escherichia coli BL21 (DE3). The sequence analysis revealed that the two enzymes are water-forming Noxs with 64 % and 52 % identity to LbNox from Lactobacillus brevis DSM 20054. The optimal pH and temperature of the purified LpNox1 and LpNox2 were 7.0 and 8.0 and 35 and 40 °C, respectively, with K
M of 99.0 μM (LpNox1) and 27.6 μM (LpNox2), and yielding catalytic efficiency k
cat / K
M of 1.0 and 0.2 μM −1 s −1 , respectively. Heat inactivation studies revealed that the two enzymes are relatively instable. The application of LpNox1 for the regeneration of NAD + was demonstrated by coupling with a glycerol dehydrogenase-catalyzed oxidation of glycerol to 1,3-dihydroxyacetone. The characteristics of the LpNox1 could prove to be of interest in industrial application such as NAD + regeneration in dehydrogenase-catalyzed oxidations.
研究领域:
生物化学
·
微生物学
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DOI
PubMed
高影响因子
FOOD CONTROL
·
2020
·
IF 4.258
Xuefei Shao,Baocai Xu,Hui Zhou,Conggui Chen,Peijun Li
The mechanism of decreasing N- nitrosodimethylamine (NDMA) by Lactobacillus pentosus R3 isolated from Chinese dry sausage was assessed in a model system. The model system was devised using a DeMan-Rogosa and Sharp-ferrous sulfate broth supplement with NDMA or its precursors. The results showed that inoculating L. pentosus R3 could significantly decrease the content of NDMA ( P < 0.05). The NDMA precursors (dimethylamine and putrescine) were found to be decreased by L. pentosus R3 ( P < 0.05). Nitrite was degraded to nitrosonium cation by acidolysis, which may promote the formation of NDMA. Direct degradation of NDMA by L. pentosus R3 was also observed. The strain exhibited antioxidant activities , including Fe 2+ chelating activity, which may be responsible for inhibiting the formation reaction and decreasing the precursors of NDMA. Thus, L. pentosus R3 decreased the content of NDMA possibly by three ways: inhibition of the formation reaction, decrease in precursors (DMA and putrescine) and direct degradation. It is interesting to understand the mechanism of decreasing NDMA by L. pentosus R3 to improve the safety of fermented meat products .
研究领域:
食品科学
·
微生物学
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DOI
高影响因子
ANNALS OF MICROBIOLOGY
·
2017
·Vol. 68
·期 1
·27-34
·
IF 1.122
Xiao Yaqing,Li Peijun,Xu Mei,Wang Wu,Chen Conggui
The objective of this study was to evaluate the ability of five strains of meat-borne bacteria to decrease N-nitrosodimethylamine (NDMA) and N-nitrosodiethylamine (NDEA) and to elucidate the mechanism in Mann-Rogosa-Sharp (MRS) broth. Lactobacillus pentosus R3 was found to be the most effective in decreasing the concentration of the two N-nitrosamines (NAs) in MRS broth, with a rate of 22.05% for NDMA and 23.31% for NDEA. The concentration of the two NAs could not be reduced by either extracellular metabolites or intracellular extracts of Lb. pentosus R3 (P > 0.05), and proteinaceous substances in the cell debris were found to be responsible for the decrease. These were surface-layer proteins (SLPs) located on the cell wall. Therefore, the decrease in NDMA and NDEA by Lb. pentosus R3 is associated with its SLPs. Lb. pentosus R3 may be developed as a starter culture in the production of fermented foods with lower NAs.
研究领域:
食品科学
·
微生物学
·
毒理学
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DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2026
·Vol. 14
·期 2
·121745
·
IF 7.5
Jing Peng,Qianzi Liu,Dumengfei Chen,Li Shen,Guanzhou Qiu,Hongbo Zhao,Yuguang Wang,Zhu Chen
Coal fly ash (CFA) disposal poses environmental concerns, yet offers a source for valuable metal recovery. This study introduces a sustainable heterotrophic bioleaching approach utilizing the citrus peel autoclave liquor as a novel and economical carbon source. Hydrothermal pretreatment of citrus peel yielded a liquor rich in glucose (14.9 g/L), fructose (18.4 g/L), and organic acids like citric, gluconic, and malic acid. This liquor effectively supported the growth of two previously verified strains with outstanding bioleaching performance ( Thermotoga sedimenticola and Lactobacillus pentosus ). L. pentosus demonstrated superior metal extraction from CFA, achieving efficiencies of 66.2 % for Zn, 40.1 % for Sr, and 11.8 % for V at 3 wt% pulp density. The innate buffering capacity of the citrus peel autoclave liquor was essential for maintaining a near-neutral pH at high pulp densities (20 wt%), counteracting the high alkalinity of CFA and sustaining L. pentosus ' bioleaching activity. Conversely, under similar high pulp density, pure sugar media failed to support the growth of L. pentosus or effectively leach metals due to the extreme alkalinity. This research successfully validates citrus peel autoclave liquor as an effective and economical substrate for valuable metal recovery from CFA, offering a promising "waste-to-value" strategy and an alternative to conventional methods for CFA bioleaching.
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Li Liu,Tianhong Liu,Yuanhui Zhao,Mingyong Zeng,Xinxing Xu
The study investigates the impacts of indigenous bacterial strains inoculation specifically L. pentosus , on the flavor characteristics, microbial composition, and metabolite profiles of fermented oyster hydrolysates. This research aimed to elucidate potential mechanisms underlying the reduction of off-flavors in fermented hydrolysates. A total of 46 and 57 volatile compounds were detected by GC–MS and GC-IMS in hydrolysates inoculated with different core microbes, respectively. The 9 key volatile compounds detected by GC–MS analysis. ( E , E )-2,4-heptadienal, heptanal, octanal, pentanal, and ( E )-2-octenal reduced the off-flavor of the fermented oyster hydrolysate. Meanwhile 1-octen-3-ol, 3-octanone, 4-octanone, and ( E , Z )-2,6-nonadienal enhanced the direct contribution of desirable flavors. Variation in 16 amino acids, 10 organic acids and 3 nucleotides were monitored to further understand the metabolic changes affecting flavor quality. Moreover, pyruvate decarboxylase [EC 4.1.1.1], phosphomannanase [EC 3.2.1.109], lipoyl-CoA synthetase [EC 6.3.2.4], and arginine kinase [EC 2.7.3.3] were the main microbiologically active enzymes. An increase in the content of aromatic compounds and a decrease in the content of C6-C9 unsaturated aldehydes through Lys, Phe, Asp, Glu, phosphoenolpyruvate, oleic acid, and linoleic acid metabolism pathways improved the flavor of oyster hydrolysates fermented by L. pentosus . This research provides a theoretical basis for leveraging autochthonous microbial fermentation to systematically improve flavor characteristics in fermented products.
研究领域:
食品科学
·
微生物学
·
代谢组学
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DOI
PubMed
高影响因子
Bioresources and Bioprocessing
·
2021
·Vol. 8
·期 1
·1-17
·
IF 4.578
Wang Jianfei,Guo Huanyu,Huang Jiaqi,Jiang Shaoming,Hou Shibo,Chen Xingyu,Lv Hujie,Bi Xudong,Hou Maolin,Lin Hebei,Lu Yuming,Qiao Jinyue,Yang Ruiyi,Liu Shijie
Under the optimal conditions of immobilization and fermentation, the highest LA yield of 0.966 ± 0.006 g/g fructose and production rate of 2.426 ± 0.018 g/(L × h) with an error of -0.5% and -0.2% to the predicted results were obtained from batch fermentation by the CS film-coated SA-PVA immobilized L. pentosus cells. The LA yield and production rate of these immobilized cells were 2.7% and 10.1% higher than that of normal SA-PVA immobilized cells respectively, and they were 5.7% and 48.4% higher than that of free cells, respectively. The effect of temperature on different types of immobilized cells and free cells was significantly different, but the effect of pH on different types of cells was not much different. The kinetic models could effectively describe the different fermentation performances of three types of cells. The immobilized cells have excellent reusability to conduct 9 runs of repeated batch fermentation.
研究领域:
生物技术
·
微生物发酵
·
生物化学工程
·
应用微生物学
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Fang Liu,Jie Bai,Wenkang Huang,Fuhou Li,Wencan Ke,Yixin Zhang,Dongmei Xie,Bo Zhang,Xusheng Guo
In this study, a novel beta-cypermethrin (beta-cyp)-degrading strain Lactobacillus pentosus 3–27 (LP3–27) was screened from beta-cyp-contaminated silage. The strain could degrade 96% of beta-cyp (50 mg/L) in MSM medium after 4 d of culture, while the strain lost its degradation ability when the beta-cyp concentration reached 250 mg/L. The effects of LP 3–27 on fermentation, bacterial community, and bioremediation of contaminated alfalfa silage at two dry matter (DM) contents were studied. The results showed that inoculation with LP3–27 not only degraded beta-cyp, but also improved the fermentation quality of alfalfa silage after 60 d of ensiling. Meanwhile, L. pentosus dominated the bacterial community during ensiling in LP3–27 inoculated silages, whereas Pediococcus acidilactici was the dominant species in the control silage. LP3–27 inoculation also simplified the bacterial interaction networks of ensiled alfalfa. Beta-cyp degradation was positively correlated with L. pentosus in LP- inoculated silages, which confirmed the function of beta-cyp degradation by L. pentosus . In addition, higher beta-cyp degradation was observed in silage with 35% versus 43% DM. In summary, strain LP3–27 could be used as a candidate inoculum for bioremediation of beta-cyp-contaminated silage and to produce safe silage for animal production.
研究领域:
微生物学
·
环境科学
·
动物营养学
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DOI
PubMed
高影响因子
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