Journal of Functional Foods
·
2025
·
IF 4
Zhaoxi Deng,Yanfei Ma,Kangwei Hou,Jinyan Dai,Yifan Zhong,Yingying Mao,Jinzhi Zhang,Haifeng Wang
Quorum sensing (QS) is an essential pathway for bacterial communication through autoinducers (AIs). The luxS gene involved in QS has been proven to regulate different behavior of Lactobacillus rhamnosus GG (LGG), whereas it is unclear whether the AI-2/LuxS system have beneficial effects the host by regulating LGG. Our aim was to investigate whether luxS mutation affects the ability of LGG to maintain intestinal microbiota homeostasis, intestinal barrier integrity and inflammatory immune responses in mice infected with Escherichia coli ( E. coli ). This study showed that both wild-type (WT) and luxS mutant (ΔluxS) of LGG can alleviate changes in the microbiota caused by E. coli by down-regulating the relative abundance of Parasturtella . Supplementation with WT and ΔluxS increased the CD4 + /CD8 + ratio in spleen, improved goblet cell numbers and enhanced Muc 2 secretion. Moreover, WT and ΔluxS decreases proinflammatory mediator levels by inhibiting MAPK and NF-κB signal transduction.
研究领域:
微生物学
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免疫学
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分子生物学
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产品关联
DOI
高影响因子
Frontiers in Microbiology
·
2019
·
IF 4.259
Gao Jie,Li Yubin,Wan Yu,Hu Tongtong,Liu Liting,Yang Shaojie,Gong Zelong,Zeng Qing,Wei Yi,Yang Weijun,Zeng Zhijie,He Xiaolong,Huang Sheng-He,Cao Hong
It has long been known that probiotics can be used to maintain intestinal homeostasis and treat a number of gastrointestinal disorders, but the underlying mechanism has remained obscure. Recently, increasing evidence supports the notion that certain probiotic-derived components, such as bacteriocins, lipoteichoic acids, surface layer protein and secreted protein, have a similar protective role on intestinal barrier function as that of live probiotics. These bioactive components have been named ‘postbiotics’ in the most recent publications. We previously found that the Lactobacillus rhamnosus GG (LGG) culture supernatant is able to accelerate the maturation of neonatal intestinal defense and prevent neonatal rats from oral Escherichia coli K1 infection. However, the identity of the bioactive constituents has not yet been determined. In this study, using liquid chromatography-tandem mass spectrometry analysis, we identified a novel secreted protein (named HM0539 here) involved in the beneficial effect of LGG culture supernatant. HM0539 was recombinated, purified, and applied for exploring its potential bioactivity in vitro and in vivo. Our results showed that HM0539 exhibits a potent protective effect on the intestinal barrier, as reflected by enhancing intestinal mucin expression and preventing against lipopolysaccharide (LPS)- or tumor necrosis factor α (TNF-α)-induced intestinal barrier injury, including downregulation of intestinal mucin (MUC2), zonula occludens-1 (ZO-1) and disruption of the intestinal integrity. Using a neonatal rat model of E. coli K1 infection via the oral route, we verified that HM0539 is sufficient to promote development of neonatal intestinal defense and prevent against E. coli K1 pathogenesis. Moreover, we further extended the role of HM0539 and found it has potential to prevent dextran sulfate sodium (DSS)-induced colitis as well as LPS/D-galactosamine-induced bacterial translocation and liver injury. In conclusion, we identified a novel LGG postbiotic HM0539 which exerts a protective effect on intestinal barrier function. Our findings indicated that HM0539 has potential to become a useful agent for prevention and treatment of intestinal barrier dysfunction- related diseases.
研究领域:
微生物学
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胃肠病学
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免疫学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROBIOLOGICAL RESEARCH
·
2024
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IF 6.1
Li-Li Zhang,Jia-Ying Xu,Yifei Xing,Pengcheng Wu,Yi-Wen Jin,Wei Wei,Lin Zhao,Jing Yang,Guo-Chong Chen,Li-Qiang Qin
Radiation injury to the intestine is one of the most common complications in patients undergoing abdominal or pelvic cavity radiotherapy. In this study, we investigated the potential protective effect of Lactobacillus rhamnosus GG (LGG) on radiation-induced intestinal injury and its underlying mechanisms. Mice were assigned to a control group, a 10 Gy total abdominal irradiation (TAI) group, or a group pretreated with 10 8 CFU LGG for three days before TAI. Small intestine and gut microbiota were analyzed 3.5 days post-exposure. LGG intervention improved intestinal structure, reduced jejunal DNA damage, and inhibited the inflammatory cGAS/STING pathway. Furthermore, LGG reduced M1 proinflammatory macrophage and CD8+ T cell infiltration, restoring the balance between Th17 and Treg cells in the inflamed jejunum. LGG also partially restored the gut microbiota. These findings suggest the possible therapeutic radioprotective effect of probiotics LGG in alleviating radiation-induced intestinal injury by maintaining immune homeostasis and reshaping gut microbiota.
研究领域:
放射生物学
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免疫学
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微生物学
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胃肠病学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2025
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IF 8.2
Hafza Behroz Mehvish,Shirong Zhang,Yi Liang,Da Sun,Xu Qiu,Kai Qu,Xian Qin,Jiawei Li,Fei Yan,Chunhui Lang,Lixin Xu,Guixue Wang,Jian Chen,Bo He,Kun Zhang,Wei Wu
Osteoporosis is the most common systemic skeletal disorder, particularly associated with aging and postmenopausal women. With the growing knowledge about the gut–bone axis, the therapeutic strategies for osteoporosis have been shifted toward regulating gut microbiota to promote positive bone metabolism. AlthoughLactobacillus rhamnosus GG (LGG) is widely reported to positively regulate bone metabolism by restoring the dysbiotic microbiome, oral administration is associated with sensitivity to gastric fluid and low bioavailability. Other studies also demonstrated that bisphosphonates ameliorate osteoporosis by directly regulating bone metabolism, especially inhibiting osteoclast activity. However, the side effects caused by bisphosphonate treatment still represent a significant problem. In this study, we assembled alendronate, a clinically used bisphosphonate, with DSPE-phospholipid nanoencapsulation and LGG (ADB), to protect LGG from the acidic environment of the stomach, while simultaneously reducing the gastrointestinal side effects associated with the oral administration of alendronate. We further investigated the potential of these DSPE-phospholipid nanoencapsulated bacteria ADB to repair osteoporotic bone deterioration, and their ability to regulate gut microbiota in vivo, which is strongly associated with the intrinsic environment. Compared with the same dosage of LGG and alendronate alone, ADB positively regulated bone metabolism, and osteoporosis was significantly revised in ovariectomized mice models.
研究领域:
医学
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药理学
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
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2023
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IF 6.1
Ge Guo,Su-Hui Xu,Yi-Tian Du,Tie-Min Jiang,Jia-Le Song,Zhen-Quan Yang,Ya-Jun Gao
In this paper, the potassium cobalt hexacyanoferrate (II), K 2 CoFe(CN) 6 , with peroxidase-like activity was used for the fabrication of a novel label-free Lactobacillus rhamnosus GG (LGG) electrochemical immunosensor. The K 2 CoFe(CN) 6 nanocubes were made by a simple hydrothermal method and followed by low-temperature calcination. In addition to structural characterization, the peroxidase-mimicking catalytic property of the material was confirmed by a chromogenic reaction. It is known that H 2 O 2 can oxidize electroactive thionine molecules under the catalysis of horseradish peroxidase (HRP). In this nanozyme-based electrochemical immunoassay , due to the steric hindrance , the formation of immune-complex of LGG and LGG antibody on the modified GCE inhibits the catalytic activity of the peroxidase mimics of K 2 CoFe(CN) 6 and thus reduced the current signal. Therefore, the developed electrochemical immunosensor achieved quantitative detection of LGG. Under optimal conditions, the linear range of the sensor was obtained from 10 1 to 10 6 CFU mL −1 with a minimum detection limit (LOD) of 12 CFU mL −1 . Furthermore, the immunosensor was successfully applied in the quantitative detection of LGG in dairy product samples with recoveries ranging from 93.2% to 106.8%. This protocol presents a novel immunoassay method , which provides an alternative implementation pathway for the quantitative detection of microorganisms.
研究领域:
生物化学
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免疫学
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微生物检测
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产品关联
DOI
PubMed
高影响因子
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
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2022
·Vol. 38
·期 12
·1-14
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IF 4.253
Wang Ju,Yin Lifen,Zheng Wenxiu,Shi Shengnan,Hao Wenzhuo,Liu Changhong,Zheng Lei
Intestinal peristalsis is essential for gastrointestinal function, which could maintain the appropriate progression and digestion of food and reduce bacterial aggregation through mixing function. Even though certain ingredients of foodstuff are known to increase or decrease intestinal peristalsis, the role of environmental pollutants on intestinal peristalsis is relatively unknown. Therefore, the effects of four typical environmental pollutants (oxytetracycline, arsenic, polychlorinated biphenyls and chlorpyrifos) on intestinal peristalsis in the zebrafish model and then tested the recovery effect of the constipation-resistant probiotic. The results showed that 4-day environmental pollutants exposures on the zebrafish embryos at 1 day post fertilization clearly decreased the intestinal peristalsis through decreasing the serotonin (5-HT) production and down-regulating the expression of key genes involved in 5-HT synthesis. Pollutants-evoked change of gut motility could be normalized in the presence of Lactobacillus rhamnosus GG (LGG) via increasing 5-HT secretion. Exogenous 5-hydroxytryptophan (100 µg/L) could also rescue the dysfunction of gut motility in pollutants-treated zebrfish. The data identified that LGG normalized disorder of intestinal peristalsis induced by environmental pollutants through increasing 5-HT level. The stimulant effect of LGG on peristalsis may be associated with 5-HT system, which could provide references for the application of probiotics in regulation of gut dysmotility. Graphical abstract
研究领域:
环境毒理学
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胃肠病学
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微生物学
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产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
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2023
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IF 4.4
Yu-Chi Tseng,Chengfeng Xue,I-Son Ng
The abundance and biodiversity of intestinal bacteria play a critical role in immune system. Escherichia coli Nissle 1917 (EcN), a tractable probiotic cell has emerged as a ‘smart microbe’ that can provide therapeutic modalities at the sites of disease. Additionally, Lactobacillus rhamnosus GG (LGG) represents one of the key probiotics in clinical studies with the ability to enhance intestinal immunity in symbiosis with other cells. To fully exploit the advantages of EcN and LGG, we optimized the commensal culture medium for LGG and EcN, succeeded by the establishment of a novel fluorescence-based colony quantification. From the metabolite analysis, more lactate was consumed when LGG is co-cultured with EcN; thus, YkgG was further explored as it was foreseen to be the enzyme responsible for lactate conversion to pyruvate. The YkgG enzyme from EcN has V max of 64.5 mM min -1 and catalytic ability k cat /K m of 2055 mM -1 min -1 , that is 6-fold compared to E. coli MG1655. Co-culture strategy of EcN with LGG in the mimic gastrointestinal tract increased the CFU by 50 % and 40 % within 5 mins and 10 mins, respectively. This practical, feasible, and promising symbiosis system aims to accelerate the application of probiotic in healthy food industries.
研究领域:
微生物学
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免疫学
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食品科学
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产品关联
DOI
高影响因子
Food Analytical Methods
·
2022
·Vol. 15
·期 12
·3379-3389
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IF 3.498
Wang Jiamin,Zhou Wenyuan,Gao Lu,Yang Zhenquan,Yang Zhanjun,Chen Dawei,Gao Yajun
In this paper, we have developed a fast and low-cost label-free electrochemical immunosensor for detection of Lactobacillus rhamnosus GG (LGG). The proposed sensor is based on a glassy carbon electrode modified with Cu@Cu 2 O nanoparticles anchored B, N co-doped porous carbon (BNPC) composite. The copper-derived nanoparticles with multiple valence states undergo dramatic changes in surface areas, conductivity, and actives sites due to their combination with BNPC, resulting in enhanced electrochemical performance. In the amperometric immunoassay, the immune complex of antigen and antibody inhibits the transfer of electrons on the electrode interface, causing a change in the current. Thus the quantitative detection of LGG is achieved. Differential pulse voltammetry (DPV) was performed at a LGG concentration of 10 2 to 10 8 colony-forming units CFU mL −1 . The immunosensor showed a good linear relationship between the decrease in peak current and the concentration of LGG ( R 2 = 0.9934), with a short detection time and a detection limit of 12 CFU mL −1 . In short, we have developed a highly sensitive electrochemical immunosensor for the specific detection of LGG using the current-mode, label-free immunoassay method evaluated in this study, providing a reliable mean for quantifying probiotics.
研究领域:
微生物学
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分析化学
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材料科学
✓ 已验证
DOI
高影响因子
Nanomaterials
·
2021
·Vol. 11
·期 3
·803
·
IF 5.076
Penghui Nie,Mengqi Wang,Yu Zhao,Shanji Liu,Ling Chen,Hengyi Xu
The potential toxicity of titanium dioxide nanoparticles (TiO2NPs) to mammals has become a widespread concern. Young individuals exposed to TiO2NPs have a higher risk than adults. In this study, the protective effects ofLactobacillus rhamnosusGG (LGG) on liver toxicity in young rats induced by TiO2NPs were explored. Results show that the four-week-old rats that underwent LGG after the oral intake of TiO2NPs could prevent weight loss, reduce hematological indicators (WBC and NEUT) and serum biochemical indicators (AST, ALT, AST/ALT, and ALP). Moreover, it alleviated the pathological damage of the liver (as indicated by the disordered hepatocytes, more eosinophilic, ballooning degeneration, and accompany with blood cells), but it did not reduce the Ti contents in the liver. In addition, RT-qPCR results indicated that LGG restored the expression of anti-oxidative stress-related genes, such asSOD1,SOD2,CAT,HO-1,GSH,GCLC, andGCLMin the liver. In summary, the hepatotoxicity of TiO2NPs in young rats is closely related to oxidative stress, and the antioxidant effect of LGG might protect the harmful effects caused by TiO2NPs.Keywords:titanium dioxide nanoparticles;Lactobacillus rhamnosusGG;liver;toxicity;oxidative stress
研究领域:
毒理学
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微生物学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
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IF 7.7
Yan Hu,Shengpeng Zhu,Xuexin Ye,Zhijie Wen,Hudie Fu,Jiasi Zhao,Mohan Zhao,Xinxi Li,Yuqing Wang,Xiaojun Li,Li Kang,Ainiwaer Aikemu,Xinzhou Yang
Probiotics regulate intestinal flora balance and enhance the intestinal barrier, which is useful in preventing and treating colitis. However, they have strict storage requirements. In addition, they degrade in a strongly acidic environment, resulting in a significant decrease in their activity when used as microbial agents. Lactobacillus rhamnosus GG (LGG) was loaded into acid-resistant and colon-targeting double-layer microgels. The inner layer consists of guar gum (GG) and low methoxyl pectin (LMP), which can achieve retention and degradation in the colon. To achieve colon localization, the outer layer was composed of chitosan (CS) and sodium alginate (SA). The formulation demonstrated favorable bio-responses across various pH conditions in vitro and sustained release of LGG in the colon lesions. Bare LGG survival decreased by 52.2 % in simulated gastric juice (pH 1.2) for 2 h, whereas that of encapsulated LGG decreased by 18.5 %. In the DSS-induced inflammatory model, LGG-loaded microgel significantly alleviated UC symptoms in mice and reduced inflammatory factor levels in the colon. Encapsulation of LGG improved its stability in acidic conditions, thus increasing its content at the colon lesions and reducing pathogenic bacteria. These findings provide an experimental basis and a technical reference for developing and applying probiotic microgel preparations.
研究领域:
药理学
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微生物学
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胃肠病学
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANIMAL FEED SCIENCE AND TECHNOLOGY
·
2023
·
IF 3.2
Lijie Zhang,Chuankai Zhang,Mingming Song,Danqi Dang,Jianan Zhao,Liyang Zhang,Tong Fu
The aim of the present study was to characterize the fermentation properties of Lactobacillus rhamnosus GG (LGG; ATCC 53103) in vitro, and investigate the effects of inoculation with LGG on fermentation quality of whole plant corn silage (WPCS) and fermented total mixed ration (FTMR). The antifungal activity of LGG against 4 prevalent fungi isolated from corn stalk was determined using the Oxford cup method. The binding capacity of LGG to aflatoxin B 1 , zearalenone and deoxynivalenol was examined by in vitro adsorption experiments using a batch mode in phosphate buffered saline. The antioxidant activity of LGG in vitro was evaluated by determining the resistance to hydrogen peroxide, free radical scavenging capacity and reducing activity. Three batches of whole plant corn and total mixed ration were collected separately. Each batch of raw material was divided into equal portions and applied to 2 treatments: (1) control with distiller water (CON), and (2) treatment inoculated with LGG (LGG). After 48 d of ensiling, the chemical composition, ruminal degradability, microbial community and mycotoxin concentrations of WPCS and FTMR were determined. The results showed that lactic acid was the predominant component among organic acids in LGG culture process, with a rapid generated occurring at 4 h after inoculation and reaching a stable state at approximately 48 h. Lactobacillus rhamnosus GG could effectively inhibit the growth of 4 prevalent fungi isolated from corn stalk, the diameter of inhibition zone was higher for Mucor circinelloides than that of the other moulds ( P = 0.016). With increasing of LGG cell amount, the binding capacity to 3 mycotoxins increased ( P < 0.01); the heat and acid treated LGG also enhanced the binding capacity to mycotoxins ( P < 0.01). Lactobacillus rhamnosus GG displayed tolerance to hydrogen peroxide (0.8 mmol/L) for 8 h at 37 °C (96%, viability), but the viability decreased sharply (52.39%, viability) under the concentration of H 2 O 2 increased to 1.2 mmol/L ( P < 0.001). The free radical scavenging activity and reducing activity of viable LGG were greatest, followed by cell-free extracts and inactivated cells ( P < 0.001). Ensiling of WPCS and FTMR inoculated with LGG decreased dry matter loss ( P < 0.05), increased lactic acid and acetic acid production ( P < 0.05), enhanced some nutrients ruminal degradability ( P < 0.05). Meanwhile, the inoculation of LGG reduced the counts of yeast, mold and coliform bacteria ( P < 0.05) and, more importantly, alleviated mycotoxin contamination ( P < 0.05). These results indicated that LGG could be a novel inoculant to improve the quality and safety of WPCS and FTMR.
研究领域:
微生物学
·
动物营养
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2020
·
IF 4.872
Yu Zhao,Yizhou Tang,Ling Chen,Sidi Lv,Shanji Liu,Penghui Nie,Zoraida P. Aguilar,Hengyi Xu
Human were given a lot of opportunities to ingest TiO 2 NPs in the environment. Children have low, sensitive intestinal tolerance, and they could be exposed to higher levels of TiO 2 NPs than adults. Few studies have been conducted on the interaction between TiO 2 NPs and juvenile intestine phase models. Thus, in this work, weaning rats were orally exposed to TiO 2 NPs for 7 and 14 days. Results indicate that Ti accumulated in the intestine, liver, and feces. Inflammatory infiltration damage was observed in the colonic epithelial tissue, and gut microbiota fluctuated with a decreased abundance of Lactobacilli in feces. Oral supplementation with Lactobacillus rhamnosus GG (LGG) lessened TiO 2 NPs-induced colonic inflammatory injury, which might due to downregulation of nuclear factor kappa-B (NF-κB). Meanwhile, LGG maintained normal intestinal microbiome homeostasis, thereby improving TiO 2 NPs-induced colon injury in juvenile rats. Moreover, fecal microbiota transplant (FMT) experiment indicated possible TiO 2 NPs-induced intestinal microbiota disorder led to colonic inflammation. Our works suggested the urgent need for additional studies on the risk safety assessment, mechanism, and prevention of juvenile health damage from exposure to TiO 2 NPs.
研究领域:
毒理学
·
微生物学
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儿科
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营养科学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）