Frontiers in Marine Science
·
2024
·
IF 2.8
Cui Kun,Zhang Hanle,Yun Biao,Wang Jianxue,Qian Xueqiao,Xue Min
We investigated the effects of dietary supplementation with sodium propionate (SP) and tributyrin (TB) on hepatic lipid deposition and antioxidant capacity of spotted seabass (Lateolabrax maculatus) via an 8-week feeding experiment and a hypoxia stress experiment. The fish were fed five experimental diets: a control diet (CON), a diet supplemented with 2 g/kg SP (SP-0.2%), 4 g/kg SP (SP-0.4%), 2 g/kg TB (TB-0.2%), or 4 g/kg TB (TB-0.4%). No significant difference in growth performance was presented among the groups (P > 0.05). The SP-0.4% and TB-0.2% groups presented significantly lower hepatosomatic and viscerasomatic indexes compared with the CON group. Then, the SP-0.4% and TB-0.2% groups presented stronger resistance to hypoxic stress than the other groups and were analyzed further. The hepatic histology and triglyceride levels revealed that SP-0.4% and TB-0.2% reduced hepatic lipid deposition. Similarly, the downregulation of malondialdehyde and the upregulation of total antioxidant capacity, superoxide dismutase, and catalase activities and the related gene expression levels revealed that SP-0.4% and TB-0.2% improved the antioxidant capacity. Additionally, the RNA sequencing demonstrated that SP-0.4% and TB-0.2% regulated gene expression to a similar extent. Among the 117 differentially expressed genes, 67 genes were enriched in the same pattern, and involved the FoxO signaling, PI3K-Akt signaling, and insulin-related pathways. In conclusion, supplementing SP-0.4% and TB-0.2% as feed additives effectively improved hepatic lipid metabolism, antioxidant capacity, and hypoxic stress resistance of spotted seabass.
研究领域:
水产养殖
·
营养学
·
分子生物学
·
生理学
✓ 已验证
产品关联
DOI
高影响因子
AQUACULTURE
·
2024
·
IF 3.9
Tian Xia,Xiao-jiang Mao,Jian Zhang,Samad Rahimnejad,Kang-le Lu
Endoplasmic reticulum (ER) stress and mitochondrial impairment commonly occur during the progression of fatty liver disease. There is a close connection and crosstalk between mitochondria and ER. The potential positive influence of mitigating ER stress (ERS) and mitochondrial damage in the context of preventing and treating fatty liver was explored in spotted seabass ( Lateolabrax maculatus ) as a susceptible model to fatty liver. A normal-fat diet (11% fat, NFD) and a high-fat diet (17% fat, HFD) were formulated and three additional diets were prepared by supplementation of quercetin (QUE) (0.1%), hydroxytyrosol (HT) (0.02%) or their combination (0.1% QUE + 0.02% HT) to the HFD (HFD + QUE HFD + HT and HFD + QUE + HT diets).Three replicate groups of fish (25 ± 0.02 g) were fed the test diets to visual satiety for 8 weeks. The results revealed the deterioration of growth, feed intake and feed utilization in HFD group compared to the NFD group whereas QUE and HT application improved their values and the optimum results were achieved for the HFD + QUE + HT group. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities, and both serum and hepatic triglyceride (TG), total cholesterol (T-CHO) and non-esterified free fatty acids (NEFA) concentrations were enhanced in HFD group, and their values decreased by QUE and HT, particularly their combined supplementation. The results of oil red O staining exhibited a remarkable reduction in number of lipid droplets in the liver of fish from HFD + QUE + HT group compared to the HFD group. Whole-body and muscle fat contents and abdominal fat index decreased by QUE and HT application. Expression of lipid metabolism related genes ( srebp-1c, fas, acc ) were down-regulated by QUE and HT treatment. Co-supplementation of QUE and HT down-regulated the expression of ERS-related genes ( grp78, chop, perk, atf6, ire1 ), improved ER ultrastructure, and enhanced the coupling between mitochondria and ER. Moreover, expression of genes associated with hepatic mitochondrial biogenesis ( pgc1-α, pgc1-β, nrf1 ) and mitophagy ( pink1, atg5, mul1 ) was up-regulated in HFD + QUE, HFD + HT and HFD + QUE + HT groups. These data suggest that combined supplementation of QUE and HT in HFD for spotted seabass can improve growth and feed utilization, reduce hepatic damage and fat accumulation, and alleviate HFD induced ERS and mitochondrial dysfunction.
研究领域:
水产养殖
·
营养学
·
生理学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Animal Nutrition
·
2024
·
IF 6.1
Xiao Li,Jing Li,Kangle Lu,Xueshan Li,Kai Song,Ling Wang,Chunxiao Zhang
A 10-week feeding trial, followed by 24-h nitrite stress, was performed to evaluate the effects of dietary selenium-L-methionine (Se-Met) on growth, Se accumulation, antioxidant capacity, transcripts of selenoproteins and histological changes of muscle as well as resistance to nitrite stress in spotted seabass ( Lateolabrax maculatus ) reared at optimal (27 °C) and high (33 °C) temperatures. Five experimental diets were formulated to contain 0, 0.9, 1.8, 3.5, and 7.0 mg Se-Met/kg. Each diet was fed to fish (2.60 ± 0.2 g) in two parallel treatments at 27 or 33 °C. The results showed that elevated temperature (33 °C) induced thermal stress in fish, and fish under thermal stress exhibited lower weight gain (WG) and hepatosomatic index (HSI) but a higher condition factor (CF) compared to those reared at 27 °C. However, the growth and feed utilisation were promoted in L. maculatus with 0.9 to 3.5 mg/kg Se-Met treatments. The protein and lipid content in the muscle increased with the dietary Se-Met level, and the total Se level in the whole body and muscle showed a linear increase with dietary Se-Met supplementation. Thermal stress changed the histology of the muscle, leading to raised levels of malondialdehyde (MDA), reduced antioxidant parameters in the serum and liver, and a decrease in the transcripts of selenoprotein genes in the muscle. Meanwhile, increased antioxidant capacity of serum and liver and up-regulated transcripts of selenoprotein of muscle were observed in L. maculatus reaching a maximum with 3.5 mg Se-Met/kg treatment. After 24 h of nitrite stress, thermal stress exacerbated oxidative damage caused by nitrite stress in L. maculatus . In contrast, dietary Se-Met enhanced the resistance to nitrite stress of L . maculatus fed with Se-Met enriched diets containing 0.9 to 1.8 mg Se-Met/kg. Based on the effects of dietary Se-Met on the growth, antioxidant capacity and resistance to nitrite stress of L. maculatus , this study suggests that the optimal range of Se-Met supplementation in L. maculatus diets is 1.80 to 2.39 mg Se-Met/kg of diet at 27 °C and 1.80 to 4.46 mg Se-Met/kg of diet at 33 °C.
研究领域:
水产养殖
·
营养学
·
生理学
·
应激生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Aquatic Food Product Technology
·
2023
·
IF 1.6
Jie Zheng,Hao Ding,Lirong Bai,Shuai Zhu,Hongyuan Hui,Xinyue Zhu,Wen Chen,Aijun Hu
The Chinese traditional food, fermented semi-dried sea bass (FSSB), is famous for its unique flavor. The microbiota succession and flavor formation during fermentation remain unknown. This study utilized high-throughput sequencing to investigate the evolving microbiota and chemical determination to explore volatile compounds. The relevant abundance of microorganisms in the middle-fermented stage was significantly (p <.05) higher than that of the post-fermented stage. In addition, the main microbial phyla during fermentation were Firmicutes and Proteobacteria. In conclusion, this study contributes to understanding the changes in flavor substances during fermentation and the varying patterns of change among different floras.
研究领域:
食品科学
·
微生物学
·
化学分析
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Weiqing Lan,Mingxing Zhou,Bingjie Zhang,Shucheng Liu,Jing Xie
Phenolic acid-chitosan derivatives have received extensive attention due to their greatly enhanced mechanical, antibacterial and antioxidant properties, especially in food preservation. The chitosan-gentianic acid (CS-g-GA) was prepared and its impact on myofibrillar proteins (MPs) in sea bass ( Lateolabrax maculatus ) during refrigerated storage was investigated in this study. Fish fillets were immersed in distilled water, CS, GA and CS-g-GA solutions, respectively, followed by an 18-day refrigerated storage. MPs were extracted from fish fillets every 3 days, and then the degree of oxidation and conformational changes of MPs were evaluated. The results of myofibril fragmentation index (MFI) and surface hydrophobicity confirmed that CS-g-GA can retard the fragmentation and degradation of myofibrils. Additionally, the total sulfhydryl content of CS-g-GA-treated samples in the later storage was significantly higher than those of other groups, confirming that CS-g-GA treatment protected the structure and function of protein. Results of Fourier transform infrared spectroscopy (FT-IR) and inner fluorescence intensity (IFI) showed that CS-g-GA maintained the secondary and tertiary structure of MPs. The results from scanning electron microscope (SEM) further clarified that CS-g-GA can maintain the tight arrangement of muscle fibers and protect the microstructure of myofibrils. Furthermore, the observation of confocal laser scanning microscopy (CLSM) demonstrated that CS-g-GA can prevent protein cross-linking and aggregation. Therefore, CS-g-GA treatment can effectively protect the structure and delay the oxidation of MPs.
研究领域:
食品科学
·
蛋白质化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Xiao-jiang Mao,Xue-shan Li,Ling Wang,Kai Song,Chun-xiao Zhang,Ke-Quan Chen,Kang-le Lu
Coenzyme Q10 (COQ10), a natural fat-soluble compound, is known to protect mitochondria, though the exact mechanism remains unclear. Here, we explored the mechanism through which COQ10 enhances mitochondrial function using hepatocytes from spotted seabass ( Lateolabrax maculatus ). Three experimental groups were established: a normal medium control group, a fatty acid (FA) group containing 100 μM FA, and a group containing100 μM FA plus 5 μM COQ10 (FA + COQ10). After incubation, the results showed that FA treatment significantly increased triglyceride content in the cells, while COQ10 treatment reduced triglycerides content ( P < 0.05). Additionally, COQ10 alleviated FA-induced hepatocyte damage, restored mitochondrial membrane potential and increased ATP content ( P < 0.05). Further analysis showed that FA downregulated key mitophagy genes ( PINK1 , PARKIN , and LC3B ) while upregulating P62 ( P < 0.05). To investigate whether the enhancement of mitochondrial function by COQ10 is dependent on mitophagy activation, we used the mitophagy inhibitor Mdivi-1 and set it to FA + COQ10 + Mdivi-1 group. After mtphagy Dye staining, the number of autophagosomes in the FA + COQ10 + Mdivi-1 group was lower than in the FA + COQ10 group, indicating that the mitophagy activation by COQ10 was inhibited. Overall, these results demonstrated that COQ10 enhances mitochondrial function and alleviates excessive fat deposition in hepatocytes via PINK1-mediated mitophagy. This also indicates that COQ10 can be potentially applied to improve the metabolic disorders of fish in aquaculture.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jiaxin Zhao,Weiqing Lan,Jing Xie
In this study, the soybean protein isolate / chitosan oligosaccharide (SPI/COS) conjugates were developed using glycosylation and used as a stabilizer of carvacrol-loaded emulsions for refrigerating sea bass ( Lateolabrax maculatus ). The results of Fourier transform infrared spectroscopy and fluorescence spectroscopy showed that COS modified the secondary and tertiary structure of SPI by covalent binding. The SPI/COS conjugate with a mass ratio of SPI and COS of 4:1 (SPI/COS-4) exhibited high glycosylation degree, great water solubility and better emulsification properties. Carvacrol-loaded emulsion stabilized by the SPI/COS-4 (CE-SPI/COS-4) had nanoscale droplet size and remained stable during 40 days of storage. SPI/COS-4 aqueous solution (SPI/COS) and CE-SPI/COS-4 aqueous solution (CE-SPI/COS) in the form of immersion were applied to investigate their preservation effects on sea bass, with sterile water as control check (CK). Sea bass were stored at 4 °C and quality assessments of fish were performed periodically for up to 21 days, which included microbiological and physicochemical analysis. As suggested by microbiological analysis, CE-SPI/COS treatment could significantly inhibit the bacterial growth. The low total volatile basic nitrogen value, thiobarbituric acid value and K-value indicated that CE-SPI/COS greatly retarded protein and lipid oxidation in fish and significantly suppress the degradation of nucleotide. Moreover, fish treated with CE-SPI/COS had minimal color variation and water loss, and maintained hardness, adhesiveness, chewiness, and resilience of fish to the highest degree. Therefore, emulsions stabilized with SPI/COS conjugates may be employed as a potential strategy for the quality maintenance of sea bass.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Coatings
·
2019
·Vol. 9
·期 8
·489
·
IF 2.33
Qianqian Zhou,Peiyun Li,Shiyuan Fang,Wenru Liu,Jun Mei,Jing Xie
This research was to evaluate the effects of gelatin (G) active coating containing eugenol/β-cyclodextrin (βCD) emulsions combined with superchilling (−0.9 °C) on physicochemical, microbiological, and organoleptic properties of Chinese seabass samples during 30 days of storage. Results showed that seabass samples dipped in G-βCD coatings containing 0.15% or 0.3% eugenol combined with superchilling could significantly lower the total volatile basic nitrogen, K value, total viable count, H2S-producing bacteria,Pseudomonasspp. and Psychrophilic counts, and free fatty acids. Further, G-βCD coatings containing eugenol with superchilling (−0.9 °C) were more effective in retarding the water migration by low field NMR and MRI results, maintaining quality of seabass during storage according to organoleptic evaluation results.Keywords:Chinese seabass;superchilling;active coating;eugenol;shelf life
研究领域:
食品科学
·
水产保鲜
✓ 已验证
产品关联
DOI
高影响因子
FISH PHYSIOLOGY AND BIOCHEMISTRY
·
2025
·Vol. 51
·期 3
·1-20
·
IF 2.5
Cao Jie,Guo Meijie,Wang Jinfeng,Mei Jun,Xie Jing
Plant-based essential oils have been considered to be used as fish anesthetics and as suitable alternatives to synthetic anesthetics. In this study, we evaluated the effect of Cinnamomum camphora var. linaloofera Fujita essential oil (CFEO), MS-222 and linalool on anesthetic induction and recovery of spotted seabass, Lateolabrax maculatus , and verified their sedative effects during simulated transportation. A total of 198 live spotted seabass samples were subjected to various concentrations of MS-222, CFEO, and linalool: 10, 20, 30, 50, 100, 150, and 200 mg L −1 , individually, to evaluate the anesthetic induction time. MS-222, CFEO, and linalool all showed a deep sedation in spotted seabass at 20 mg L −1 . The minimum concentration required to achieve profound anesthesia in spotted seabass was found to be 50 mg L −1 MS-222, 100 mg L −1 CFEO, and 50 mg L −1 linalool, respectively. In addition, 120 healthy spotted seabass (400 g ± 3.5 g) were randomly divided into four groups, which were tranquilized by 20 mg L −1 CFEO, linalool, and MS-222, and the control fish were kept in clean seawater without anesthetics and essential oils. Then, the spotted seabass was simulated to be transported in a vibrating conveyor under 100 rpm at 12 °C for 24 h. The results showed that the fish transported with MS-222 and CFEO showed lower expressions in cytokine (tumor necrosis factor-α, interleukin-6, and interleukin-1 beta) compared to fish transported with linalool. Sedation with linalool reduced oxidative stress (higher superoxide dismutase, catalase, and glutathione peroxidase activities) in transport compared to the control but did not prevent changes in the activities of heat shock protein. In general, the efficacy of CFEO in anesthetizing and sedating spotted seabass and mitigating transport stress was as good as or better than that of MS-222, demonstrating it has the potential to be used as a new and natural sedation for spotted seabass during transport.
研究领域:
水产养殖学
·
药理学
·
环境生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Marine Science
·
2021
·
IF 4.912
Dong Yan-Zou,Xia Tian,Lin Ji-Bin,Wang Ling,Song Kai,Zhang Chun-Xiao
This study aimed to investigate the effects of quercetin (QUE) on fat deposition and the underlying mechanism. Fish were fed four test diets: normal fat diet (NFD), high-fat diet (HFD), and HFD supplemented with 0.5 or 1.0 g/kg quercetin (QUE0.5 or QUE1.0). The results showed that HFD feeding resulted in poor growth and feed utilization while QUE treatment reversed this. The fat contents of serum and liver were increased by HFD and QUE supplementation significantly decreased fat content. Furthermore, gene expressions and ultrastructure observation showed that mitochondrial biogenesis and mitophagy were inhibited and endoplasmic reticulum stress (ERS) in the HFD group. QUE can activate the biogenesis and autophagy of mitochondria and suppress ERS, which is related to its fat-lowering effect.
研究领域:
水产养殖
·
营养学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Coatings
·
2021
·Vol. 11
·期 2
·147
·
IF 2.881
Xuan Ma,Qianqian Zhou,Weiqiang Qiu,Jun Mei,Jing Xie
The purpose of this study was to evaluate the effect of an active gelatin coating containing eugenol and vacuum on the microbial diversity of Chinese seabass (Lateolabrax maculatus) during cold (−0.9 °C) storage. The bacterial sequences in Chinese seabass were observed using a high-throughput sequencing technique targeting the V3–V4 region of the 16S Ribosomal DNA (rDNA) on 0, 12th, and 24th day, which showed a more comprehensive estimate of the microbial diversity in seabass samples compared with microbial enumeration. The results revealed that the species diversity of fresh seabass was rich, mainly includingCarnobacterium,Glutamicibacter, andPseudomonas, with abundance ratios of 0.286, 0.160, and 0.130, respectively.PseudomonasandShewanellawere the primary contaminants in the spoiled control samples, where the abundance ratios increased from 0.220 and 0.174 on the 12th day to 0.802 and 0.163 on the 24th day, respectively. Vacuum treatment could inhibit the growth ofPseudomonasandShewanellasuch that when stored on the 12th day,Brochothrixbecame the superior genus. However,PseudomonasandShewanelladominated the storage until the 24th day, where their abundance ratios were 0.343 and 0.279, respectively. The inhibition ofPseudomonasandCarnobacteriumwas gradually enhanced with increasing concentrations of eugenol. Furthermore, an active gelatin coating containing eugenol and vacuum treatment was more effective at inhibiting the increase of the total volatile basic nitrogen. This study confirmed that an active gelatin coating containing eugenol and vacuum could reduce the species of bacteria, inhibit the growth and reproduction of the main dominant spoilage bacteria, and delay the spoilage of seabass.Keywords:coating preservation;high-throughput sequencing;shelf life;diversity
研究领域:
食品科学
·
微生物学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 18
·3292
·
IF 3.06
Shiyuan Fang,Qianqian Zhou,Yan Hu,Feng Liu,Jun Mei,Jing Xie
The objective of this research was to explore the antimicrobial activity and mechanism of carvacrol againstVibrio Parahemolyticus,Shewanella putrefaciens,Staphylococcus aureusandPseudomonas fluorescensand evaluate the effect of the addition of carvacrol/β-cyclodextrin emulsions to flaxseed gum (FSG)-sodium alginate (SA) edible films on the preservation of Chinese sea bass (Lateolabrax maculatus) fillets during refrigerated storage. The minimum inhibitory concentration (MIC) of carvacrol againstV. parahemolyticus,S. putrefaciens,S. aureusandP. fluorescenswere 0.5, 0.5, 0.125, and 0.5 mg/mL, respectively. Alkaline phosphatase activity assay, nucleotide and protein leakage, and scanning electron microscope demonstrated that carvacrol damaged the external structure of the tested bacterial cells causing leakage of cytoplasmic components. At the same time, when FSG-SA films containing carvacrol used as coating agents for Chinese sea bass fillets cold storage, FSG-SA films containing 1.0 or 2.0 mg/mL carvacrol could significantly reduce TVB-N content, K-value, the degree of microbial deterioration and maintain quality of sea bass fillets according to organoleptic evaluation results.
研究领域:
食品科学与技术
·
食品微生物学
·
活性包装
·
水产品保鲜
·
天然抗菌剂
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）