CHEMISTRY & BIODIVERSITY
·
2025
·
IF 2.5
Chaoting Wen,Zhiqiang Ye,Guoyan Liu,Li Liang,Xiaofang Liu,Youdong Li,Xin Xu,Jixian Zhang
The Lentinus edodes polysaccharide (LEP) was extracted with a new subcritical water extraction (SWE) enhanced with deep eutectic solvent (DES) method and then purified with a DEAE-52 cellulose column and a Sephadex G-100 column. Two purified polysaccharides (LEP1 and LEP2) were obtained, and their structure, antioxidant activity, and immunomodulatory activity were analyzed. LEP1 and LEP2 were composed of mannose, glucose, and galactose with a molar ratio of 1:12.97:7.84 and 1:51.18:5.29, respectively. The molecular weights were 9.878 × 10 4 and 1.976 × 10 4 Da, respectively. Interestingly, both LEP1 and LEP2 were mainly composed of →4)-β- d -Glc p -(1→, →6)-β- d -Glc p -(1→ and →6)-α- d -Gal p -(1→ with different molar ratio. Besides, both LEP1 and LEP2 had strong DPPH free radical scavenging activity and Fe 2+ chelating capacity. Moreover, they could reduce the level of reactive oxygen species (ROS) and regulate the activities of malondialdehyde (MDA), catalase (CAT), and superoxide dismutase (SOD) in HepG2 cells, demonstrating strong antioxidant activity. Furthermore, both LEP1 and LEP2 could improve the phagocytic capacity, nitric oxide (NO) release, and the content of interleukin (IL)-6, IL-1β, and tumor necrosis factor-α (TNF-α) in RAW264.7 cells, exhibiting significant immunostimulatory activity. It was worth noting that LEP2 exhibited stronger biological activities than LEP1. Therefore, the SWE enhanced with DES is an ideal method for extracting polysaccharides, which can be further applied to extract other polysaccharides. Graphical Lentinus edodes polysaccharide was extracted with a new subcritical water extraction enhanced with deep eutectic solvent method and then further purified. Two purified polysaccharides (LEP1 and LEP2) were obtained. LEP1 and LEP2 were characterized and showed significant antioxidant and immunostimulatory activity.
研究领域:
食品科学
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生物化学
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药理学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Xiaowei Huang,Chenyong Huang,Lili Zhou,Guangyue Hou,Jinyuan Sun,Xinai Zhang,Xiaobo Zou
Background Lentinan medicament from Lentinus edodes has been considered as natural medicinal products with minimal side effects for cancer therapy, but Lentinus edodes are easily polluted by nonbiodegradable heavy metals, especially silver ion (Ag + ). Therefore, it is highly desirable to monitor Ag + pollution in Lentinus edodes considering their adverse impact on lentinan medicament. Electrochemical sensor isn't affected from the interference of matrix turbidity and color, and offers a powerful means for determination of variant analytes. As for electrochemical sensing toward Ag + , there is a great need to design efficient signal probes for specific recognition and signal generation. Results We present an appropriate electrochemical aptasensor for Ag + assay based on biomimetic catalysis of porphyrin-encapsulated MOF (PorMOF) and allosteric switch of C-rich DNA . Thanks to the excellent biocompatibility, PorMOFs as nanozyme are used to design signal probes by loading duplex-like DNA scaffold. Owing to the specific recognition of Ag + toward cytosine (C) base-rich DNA, PorMOF at the distal end was close to the underlying electrode via C-Ag + -C formation, leading to an enhanced current of catalytic hydroxylamine oxidation for signal generation. Using the positive correlation between current response and Ag + level, the electrochemical system provides a promising means for on-line monitoring of Ag + in Lentinus edodes with recoveries from 92.8% to 106.4% and relative standard deviation from 3.98% to 8.24%, verifying the applicability of the electrochemical aptasensor toward Ag + in Lentinus edodes . Significance and novelty With merits of portability, simple operation, and rapid response, the electrochemical pattern offers a useful solution for on-line monitoring of Ag + in Lentinus edodes . By altering the DNA sequence , the proposed aptasensor provides a powerful way for monitoring other heavy metals, capable of protecting medicament production from heavy metal pollution.
研究领域:
环境科学
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生物医学工程
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分析化学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 8
·1872
·
IF 4.2
Daihua Hu,Yulin Wang,Fanshu Kong,Danni Wang,Chingyuan Hu,Xu Yang,Xiaohua Chen,Wang Chen,Zili Feng
Further assessment of ultraviolet C light-emitting diode (UVC-LED) irradiation for influencing shiitake mushrooms’ (Lentinus edodes) volatile and sensory properties is needed. In this study, a comparison of UVC-LED irradiation treatment on the flavor profiles in various parts of shiitake mushrooms was conducted using gas chromatography–ion mobility spectrometry (GC-IMS) and sensory analysis. Sixty-three volatile compounds were identified in shiitake mushrooms. The fresh shiitake mushrooms were characterized by the highest values of raw mushroom odors. After UVC-LED treatment, the content of C8 alcohols decreased, especially that of 1-octen-3-ol, while the content of aldehydes increased, especially the content of nonanal and decanal. The score of fatty and green odors was enhanced. For fresh samples, the mushroom odors decreased and the mushroom-like odors weakened more sharply when treated in ethanol suspension than when treated with direct irradiation. The fruit odors were enhanced using direct UVC-LED irradiation for fresh mushroom samples and the onion flavor decreased. As for shiitake mushroom powder in ethanol suspension treated with UVC-LED, the sweaty and almond odor scores decreased and the vitamin D2content in mushroom caps and stems reached 668.79 μg/g (dw) and 399.45 μg/g (dw), respectively. The results obtained from this study demonstrate that UVC-LED treatment produced rich-flavored, quality mushroom products.
研究领域:
食品科学
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农业工程
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分析化学
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感官评价
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产品关联
DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2012
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IF 2.739
Qingye Liu,Xiaojuan Xu,Lina Zhang,Junping Yu
The interaction between polydeoxyadenylic acid (poly(dA)) and single chains of Lentinan (s-LNT) was investigated by circular dichroism spectra (CD), UV–Vis spectra, nano-differential scanning calorimetry (nano-DSC), dynamic light scattering (DLS), and atomic force microscopy (AFM). All the experimental results indicated that poly(dA) really interacted with s-LNT having molecular weight of 5.3 × 10 5 to form a novel composite with stiff conformation through hydrogen bonding , whereas the triple helical Lentinan (t-LNT) could not, implying that it was the single chain but not the triple helical chain interacted with poly(dA). Meanwhile, the interaction strongly depended on the concentration of s-LNT and pH. When the poly(dA) concentration was fixed at 5.3 μg/ml, the interaction between poly(dA) and s-LNT increased with an increase in s-LNT concentration, then reached the maximum at ∼60 μg/ml, finally decreased with further increase of s-LNT concentration. This was due to self-renaturation of s-LNT into triple helix or incomplete species confirmed by nano-DSC. The poly(dA)/s-LNT complex could exist in solutions with pH 5.5–11.5, and pH 7–10 was the optimal condition for the complex, showing higher stability against pH than the positive control of poly(dA)/s-SPG. The dynamic behaviors of the complex demonstrated that the interaction between s-LNT and poly(dA) occurred rapidly, and reached stable within 3–7 h. Moreover, the thermal stability of poly(dA) was enhanced by complexation with s-LNT. The topograph of the poly(dA)/s-LNT complex was shown as rod-like stiff architecture. This work enlarges the application of LNT in the biomedical field.
研究领域:
生物化学
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分子生物学
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产品关联
DOI
高影响因子
ENVIRONMENTAL RESEARCH
·
2022
·
IF 8.431
Xiao Liu,Ziyi Shao,Yuxin Wang,Yufei Liu,Shiyao Wang,Feng Gao,Yingjie Dai
The abuse of antibiotics poses a threat to the ecological environment and biological health, and how to effectively reduce the residue of tetracycline (TC) in the environment has attracted much attention. In this study, three types of pristine biochar (BCs: PBC300, PBC500, and PBC700) were prepared using agricultural waste shiitake mushroom bran at different pyrolysis temperatures to remove TC from water. The structure and surface chemistry of the adsorbents were characterized using different analytical techniques such as scanning electron microscopy, Fourier transform infrared spectroscopy , X-ray diffraction, and thermogravimetric analysis . These changes in physicochemical properties improve the adsorption capacity of BC. The PBC300 and PBC500 conform to the Langmuir isothermal adsorption model, while the PBC700 is more compatible with the Freundlich model. According to the fitting results of the Langmuir isotherm model, the maximum saturated adsorption capacities of PBC300, PBC500 and PBC700 for TC were 7.568 mg/g, 14.994 mg/g and 17.684 mg/g, respectively. The correlation coefficients of the pseudo-second-order kinetic models were 0.9882, 0.9882 and 0.9996, respectively, which could well fit the adsorption process of TC by the three BCs, indicating that chemical adsorption was dominant. With the help of machine learning, the relationship between the physicochemical properties of BC and the adsorption capacity of TC was effectively explored. The random forest model was able to fit the adsorption process of BC on TC better. It is expected that this study will guide the rational application of BC in the treatment of TC wastewater.
研究领域:
环境科学
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生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2018
·
IF 1.206
Shuangyang Chen,Jiajia Xu,Guitang Chen,Qiuhui Hu,Liyan Zhao
Humans are exposed to arsenic by inhalation and ingestion and are therefore may be affected by its toxicity. Arsenic may enter the human body by inhalation and ingestion. Cooking may alter the contents and chemical forms of arsenic. The determination of arsenic species in Lentinus edodes after microwave blanching was performed by high-performance liquid chromatography–inductively coupled plasma–mass spectrometry. Using a physiologically based extraction, the bioaccessibility of arsenic species in raw L. edodes and microwave blanching treated L. edodes were determined after the simulated gastrointestinal digestion. The arsenate (AsV), arsenite (AsIII), monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenobetaine, and arsenocholine did not undergo decomposition and transformation in this study. Furthermore, the total contents of arsenic in L. edodes samples were in the range of 0.1378 ± 0.0044–0.2347 ± 0.0144 mg/kg. Approximately 3.38–43.27% were released from samples into the blanching water after various microwave blanching treatments. The oxidation of AsIII and demethylation of DMA and MMA were observed in L. edodes during digestion, increasing the likelihood of arsenic toxicity in the liver. The health risk for arsenic in L. edodes was decreased after microwave blanching because the potentially available arsenic in microwave blanching treatments L. edodes samples (83.78 ± 0.9103%) were lower than those in raw L. edodes samples (88.33 ± 0.7983%). L. edodes subjected to microwave blanching prior to consumption significantly decreased the total arsenic content and the risk of arsenic exposure to consumers (p < 0.05).
研究领域:
食品科学
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环境化学
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毒理学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2018
·
IF 3.909
Hongji Zhu,Li Tian,Lei Zhang,Jingxiu Bi,Qianqian Song,Hui Yang,Jianjun Qiao
This study explored the potential of spent Lentinus edodes substrate, a by-product of mushroom industries causing environmental pollution, serving as materials to produce antioxidant polysaccharide. The extraction process of spent Lentinus edodes substrate polysaccharide (SLSP) was optimized and the effects of drying methods on chemical composition, morphological property and antioxidant activity were investigated. Results showed that freeze-dried SLSP (SLSP-F) exhibited the best quality in terms of the polysaccharide yield (13.00%) and antioxidant activity. The EC 50 values of SLSP-F on DPPH, ABTS and superoxide anion radicals was 0.051 mg/mL, 0.379 mg/mL, 0.719 mg/mL, respectively, which was significantly lower than that of freeze-dried Lentinus edodes polysaccharide (LP-F). After purification by Sephadex G-150, the purified SLSP-F (PSP) has a molecular weight of 16.77 kDa. Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition. FT-IR and NMR spectroscopy analysis revealed that PSP has both α and β glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked α-D-Man p residue with some acetyl groups. The findings provided a reliable approach for the development of antioxidant polysaccharide from spent Lentinus edodes substrate.
研究领域:
农业科学
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食品科学
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生物技术
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药理学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Xian Qu,Yahui Ji,Jieyi Long,Donglin Zheng,Zhonghui Qiao,Yue Lin,Chang Lu,Yifa Zhou,Hairong Cheng
The β-1,6-glucans from Lentinus edodes have a variety of biological activities. However, the research on extraction and separation of β-1,6-glucans from L. edodes is limited and the yield is low. In the present study, we obtained the high-yield and -purity β-1,6-glucans (ALEPA) from L. edodes by using a sequential extraction and separation process, which is simple and suitable for industrialization . ALEPA significantly promoted the proliferation of splenic T lymphocytes and enhanced the phagocytosis activity of peritoneal macrophages in vivo . 16S rRNA sequencing results showed that ALEPA significantly increased the α diversity of gut microbiota and upregulated the relative abundances of short chain fatty acids (SCFAs)-producing bacterial species. Consistently, the SCFAs in the cecum of mice were upregulated. On a mechanical level, we found that the immunomodulatory effect of ALEPA depended on gut microbiota. Collectively, ALEPA is a promising functional food ingredient that regulates gut microbiota and enhances immune function.
研究领域:
食品科学
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免疫学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Heliyon
·
2020
·
IF 0
Tao Feng,Zhongshan Hu,Yanzun Tong,Lingyun Yao,Haining Zhuang,Xiao Zhu,Shiqing Song,Jun Lu
Chicken liver is a main protein source to prepare attractant for dog food. However, animal proteins are costly. Seeking high quality and low-cost protein sources has been a goal for the industry. Mushroom Lentinus edodes (L. edodes) and Mealworm Tenebrio molitor (T. molitor) are novel protein sources, showing high potential as raw material of attractants. In this paper, chicken liver, L. edodes, and T. molitor were used as three different protein sources to prepare attractants. Their palatability to dogs were then compared. Firstly, the enzymatic hydrolysis process of three proteins was optimized, with a degree of hydrolysis of 54.82%, 36.10% and 30.14% for chicken liver, L. edodes , and T. molitor respectively. Secondly, volatile compounds of three attractants were identified by HS-SPME/GC-MS and SDE/GC-MS. Using OAV and PLRS method, it was found that bis(2-methyl-3-furyl) disulfide, indole, methional, 2-(methyl thio) phenol, γ -butyrolacton, furfuryl alcohol, acetic acid and isovaleraldehyde were the key components. Although both T. molitor and L. edodes attractant showed less palatability than that of chicken liver, they could be readily improved via adding key palatable volatile compounds. The ingestion rate of dog food with attractant showed a similar trend and was higher than that of food without attractant.
研究领域:
食品科学
·
动物营养学
✓ 已验证
DOI
PubMed
Food Bioscience
·
2024
·
IF 4.8
Qinglong Kong,Songyi Lin,Menglong Sheng,Yue Geng,Zike Zhang,Dong Chen
The concept of healthiness and sustainability has promoted the innovation and development of “clean-label” products. Physico-chemical, oxidation and flavor characteristics of carp surimi with different amounts of Lentinus Edodes (LE) (0–10%) as a “clean-label” ingredient were investigated. Specifically, the color changes induced by the LE addition were not visually obvious. The 4%–10% LE addition could enhance the water holding capacity , gel strength , hardness, total reducing power and free radical scavenging rate of the samples. The cooking loss was at good levels with 6%–8% LE and negatively related to gel strength by Pearson correlation analysis. The rheological characteristics, immobilize water distribution, microstructure varied with the LE addition, and 6% addition amount was their important turning point. The total reducing power and free radical scavenging rate were positively correlated with the amounts of LE, and the minimal lipid and protein oxidation was observed at 6%–8% LE. Besides, LE addition enhanced salty and umami tastes and enriched the alcohols, aldehydes and ketones of samples. As a result, the moderate addition of LE could improve the quality of carp surimi and LE had potential to be a “clean-label” ingredient in carp surimi.
研究领域:
食品科学
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营养学
·
化学
·
农业
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Qi Kong,Honglei Mu,Yanchao Han,Weijie Wu,Chuan Tong,Xiangjun Fang,Ruiling Liu,Hangjun Chen,Haiyan Gao
In order to develop biodegradable phase change materials (PCMs) with high latent heat for cold chain logistics, superabsorbent resin (SAR) was prepared based on starch graft copolymerization. FTIR and DSC demonstrated that acrylic acid was successfully grafted onto starches and optimum latent heat of PCM was 330.4 J/g with 10% (w/w) starch. The water retention of PCM with 10% (w/w) starch was 0.49 after heating at 50 °C for 200 h, which was 4.9 folds higher than that of non-starch PCM. Biodegradation rate of PCM was 60.12% within 75-day burial, which was 6 folds higher than that of non-starch PCM. Moreover, significant reduction in browning index, odor, decay, relative conductivity and malondialdehyde (MDA) content was observed in Lentinus edodes treated by biodegradable PCM. These results indicated that the application of biodegradable PCM could extend the shelf life of fresh L. edodes stored at 25 °C.
研究领域:
食品科学
·
材料科学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yuechao Zhao,Junqi Li,Hongyu Li,Lijia Zhang,Fengshi Deng,Abdulhakeem S. Alamri,Majid Alhomrani,Fahad Al-Asmari,Wenjuan Wu,Yuntao Liu,Zhen Zeng
Lentinus edodes is widely cultivated and utilized globally, and Lentinus edodes polysaccharides (LEPs) are recognized as its primary bioactive components. However, the influence of regional variations LEPs has not yet been elucidated. This study aimed to investigate the differences in the and activity of LEPs from various sources following simulated in vitro digestion, as well as their impact on gut microbes. Our work demonstrated that while the three LEPs were partially digested during digestion progress, yet their structural integrity remained largely unchanged. These LEPs exhibited variations in the monosaccharide molar ratios and molecular weights (Mw), which contributed to differences in their biological activities. Furthermore, the three LEPs were found to promote the growth of Megasphaera and Bacteroides and enhance the production of short-chain fatty acids (SCFAs). Finally, we investigated the correlations between monosaccharide composition, SCFAs, and the growth of gut microbes. Taken together, these results provide important insights into the regional characteristics of LEPs, supporting their personalized application in various contexts.
研究领域:
食品科学
·
微生物学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）