Biomed Research International
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2022
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IF 3.246
Bingmin Wu,Yange Wang,Lishan Zeng,Wengkun Li,Lin An,Jingyan Li,Ruoting Zhan,Huasheng Kang,Li Liu,Ying Lin,Guifang Zhang
In this paper, Lignosus rhinocerotis (Cooke) Ryvarden ( L. rhinocerotis ) cultivated in rice medium (LRR) and in sawdust medium (LRS) was harvested. Then, in terms of the LRR, LRS, and wild L. rhinocerotis (LRW), the total flavonoid contents, total polyphenol contents, total polysaccharide contents, and metabolites were detected; antioxidants of their aqueous extracts and anti-inflammatory of their polysaccharides were performed. In addition, the possible mechanism of the polysaccharides of L. rhinocerotis inhibiting lung damage was elucidated. The results showed that 32 compounds were characterized in L. rhinocerotis , including flavonoids, terpenoids, lignans, and steroids and there were 20 compounds in cultivated and wild L. rhinocerotis ; LRR has the highest total polyphenol and flavonoid contents, as well as ABTS and DPPH scavenging capacity. The total polysaccharide contents and the FRAP scavenging capacity of wild L. rhinocerotis were higher than those of cultivated L. rhinocerotis . The inhibition of polysaccharides of LRW (PLRW) on LPS-induced MRC-5 damage was stronger than that of the polysaccharides from cultivated L. rhinocerotis . The PLRW may alleviate lung damage by inhibiting the NLRP3 pathway and thereby suppressing the inflammatory response. In summary, both cultivated and wild L. rhinocerotis are abundant in bioactive components and have antioxidant and anti-inflammatory activities.
研究领域:
药理学
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生物化学
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真菌学
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药物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2021
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IF 4.36
Suresh Veeraperumal,Hua-Mai Qiu,Chon-Seng Tan,Szu-Ting Ng,Wancong Zhang,Shijie Tang,Kit-Leong Cheong,Yang Liu
Ethnopharmacological relevance Lignosus rhinocerotis (Cooke) Ryvarden cultivar TM02, also known as tiger's milk mushroom, is regarded as important folk medicine in Malaysia, while is used for the treatment of liver cancer, chronic hepatitis, gastric ulcer in traditional Chinese medicine . However, there is no compilation of scientific evidence that its protection for gastric, and no attempts have been made to understand how polysaccharides in Lignosus rhinocerotis might promote intestinal mucosal wound healing. Aim of the study: This study aimed to investigate the effect and mechanism of β-glucan prepared from L. rhinocerotis using an enzymatic method on epithelial restitution during intestinal mucosal damage. Materials and methods Based on FT-IR, MALDI-TOF-MS, HPSEC-MALLS-RID, and AFM , the structure of polysaccharides from L. rhinocerotis was analysed. In addition, polysaccharides were used to test for wound healing activity in IEC-6 cells by measuring cell migration, proliferation, and expression of cell division control protein 42, Rac-1, RhoA, and Par-3. Results β-glucan was extracted using enzyme-assisted extraction, and a yield of approximately 8.5 ± 0.8% was obtained from the dried biomass. The β-glucan extracted by enzyme-assisted extraction (EAE) of polysaccharides was composed entirely of D-glucose with a total carbohydrate content of 95.5 ± 3.2%. The results of HPLC, FTIR, and MALDI-TOF-MS analyses revealed EAEP to be confirmed as β-glucan. The molecular weight of prepared β-glucan was found to be 5.315 × 10 4 g/mol by HPSEC-MALLS-RID. Furthermore, mucosal wound healing studies showed that the treatment of IEC-6 with a β-glucan concentration of 200 μg/mL promoted cell migration and proliferation, and it enhanced the protein expression of cell division control protein 42, Rac-1, RhoA, and Par-3. Conclusions The present study reveals that the prepared β-glucan accelerates intestinal epithelial cell proliferation and migration via activation of Rho-dependent pathway. Hence, β-glucan can be employed as a prospective therapeutic agent for the treatment of diseases associated with gastrointestinal mucosal damage, such as peptic ulcers and inflammatory bowel disease .
研究领域:
药理学
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细胞生物学
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胃肠病学
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民族药理学
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传统中医
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2017
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IF 4.529
Ting Hu,Qilin Huang,Kahing Wong,Hong Yang,Jingsi Gan,Yanru Li
An alkali-soluble polysaccharide was extracted from Lignosus rhinocerotis sclerotia (LRP). Its structural characteristics were determined by GC–MS, FT-IR, GC, 1D and 2D NMR combined with Smith degradation and methylation analysis. The LRP had a (1 → 3)-β- d -Glcp backbone with every three residues bearing a (1 → 6)-linked and hyperbranched side chain that contained three (1 → 6)-β- d -Glcp residues as secondary main chain and two terminal β- d -Glcp residues linked at O3. The degree of branching was 0.76 from GC–MS analysis, implying a highly branched structure for LRP. The M w , < S 2 > z 1/2 , R h and [ η ] values of LRP in 0.25 M LiCl/DMSO were measured by SEC-MALLS-Vis-RI combination technology to be 2.88 × 10 5 g/mol, 30.36 nm, 22.34 nm and 131.50 ml/g, respectively. Furthermore, the exponent α of [ η ] –M w , β of < S 2 > z 1/2 –M w , the fractal dimension d f and molecular parameter ρ were determined to be 0.20, 0.33, 2.50 and 1.36, demonstrating that the LRP was a hyperbranched polysaccharide and adopted a compact coil conformation in LiCl/DMSO.
研究领域:
生物化学
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多糖化学
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药学化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2016
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IF 3.138
Ting Hu,Qilin Huang,Kahing Wong,Hong Yang
Four polysaccharide fractions, LRP-1, LRP-2, LRP-3 and LRP-4 were extracted stepwise from Lignosus rhinocerotis sclerotia with distilled water at 25, 95, 120 °C and 1.0 M NaOH solution at 4 °C. Their structure, molecular size and chain conformation were clarified using SEC-MALLS-RI, GC, FT-IR and UV–vis. Furthermore, their immunomodulatory activities were evaluated by the model of cyclophosphamide (Cy)-induced immunosuppression. The LRP-1 and LRP-2 were polysaccharide-protein complexes (46–68% β- d -glucan and 27–48% protein), while LRP-3 and LRP-4 were absolutely composed of β- d -glucose. The LRP-4 with low polydispersity had much higher molecular weight ( M w , 5.86 × 10 6 g/mol) and intrinsic viscosity ([ η ], 202.6 ml/g) than other LRP fractions. Based on M w , radius of gyration ( z 1/2 ) and [ η ] data with the exponent β of z 1/2 –M w and its U-shaped curve, all four LRP fractions were highly branched macromolecules and LRP-3 showed a more compact sphere-like conformation than LRP-2 in aqueous solution. Additionally, all four LRP fractions exhibited protective effects against Cy-induced immunosuppression in mice by improving immune organs as well as stimulating the release of major cytokines TNF-α and INF-γ. This work provides a theoretical basis for the application of polysaccharides and their protein complexes from Lignosus rhinocerotis sclerotia in food- or drug-based therapies.
研究领域:
药理学
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免疫学
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生物化学
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真菌学
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产品关联
DOI
PubMed
高影响因子
ULTRASONICS SONOCHEMISTRY
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2017
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IF 4.218
Wenfei Cai,Ting Hu,Amr M. Bakry,Zhaomin Zheng,Yidong Xiao,Qilin Huang
The differences between ultrasonic and non-ultrasonic approaches in synthesizing Lignosus rhinocerotis polysaccharide-selenium nanoparticles (LRP-SeNPs) were compared in terms of size, morphology, stability and antioxidant activity by UV-VIS, FT-IR, X-ray diffraction (XRD), dynamic light scattering (DLS), transmission electron microscopy (TEM), and energy dispersive X-ray (EDX) with high resolution TEM. Results indicated that the SeNPs were associated with the LRP macromolecules in a physical adsorption pattern without breaking chemical bonds, and the ultrasonic treatment reduced the size of SeNPs, narrowed the size distribution as well as improved the stability. Due to the LRP compact coil structure loosed under ultrasonic cavitation, the SeNPs could be easily diffused into the LRP internal branches instead of gathering on the LRP surface and were well dispersed and eventually stabilized throughout the extended branches. After ultrasound treatment, the SeNPs had a minimum average diameter of ∼50 nm and the LRP-SeNPs could remain homogeneous and translucent for 16 days within 200 nm size. Furthermore, the ultrasound-treated LRP-SeNPs exhibited higher DPPH and ABTS radical-scavenging abilities than those untreated with ultrasound. This difference may be attributed to the reason that ultrasound can reduce the SeNPs size and increase the specific surface area, which provides sufficient active sites to react with the free radicals and suppress the oxidizing reactions. The integrated results demonstrated that ultrasound played a crucial role in the dispersion, size control, stabilization and antioxidant activity of SeNPs.
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DOI
高影响因子
FOOD CHEMISTRY
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2025
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IF 9.8
Wudan Cai,Shuai Chen,Xiao Zhang,Dan Wu,Xinyi Zhu,Honglin Zhu,Yangchao Luo,Qilin Huang
The current study aimed to investigate the effect of ultrasound assisted H 2 O 2 /Vc degradation on the digestive characteristics of Lignosus rhinocerotis polysaccharide (LRP) and gut microbiota regulation. Results showed that during in vitro digestion, no changes were observed in molecular weight ( M w ) of LRP and its antitumor activity remains stable. However, M w of degraded LRP (DLRP) slightly decreased from 45.66 × 10 4 Da to 36.61 × 10 4 Da with no free monosaccharides released, enhancing its antitumor activity. The supplementation with LRP or DLRP significantly elevated mice fecal water content and modulated microbiota composition. The total short chain fatty acids concentration of control, LRP, and DLRP groups were 20.07, 24.13, and 28.08 mmol/L, respectively. Compared with LRP, DLRP significantly increased the abundance of beneficial bacteria such as Alloprevotella and Mucispirillum , exhibiting protective effects on intestinal health. This study provides a theoretical foundation for targeted regulation of intestinal flora by LRP and DLRP.
研究领域:
食品科学
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微生物学
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药理学
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）