阿拉丁SCI论文搜索工具

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY · 2023 · IF 6.1

Response of Odorant Receptors with Phenylacetaldehyde and the Effects on the Behavior of the Rice Water Weevil (Lissorhoptrus oryzophilus)

Xinxin Zhang,Xiao Wang,Shiwen Zhao,Kui Fang,Zhun Wang,Jianan Liu,Jinghui Xi,Shang Wang,Juhong Zhang

The rice water weevil (RWW), Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae), is a destructive rice pest that threatens the rice industry worldwide. Odorant receptors (ORs) and odorant receptor coreceptors (Orcos) play an important role in the process of insects’ whole life activities; however, there are no related functional studies on RWW. On this basis, a heterologous study of LoryOR20/LoryOrco in Xenopus laevis oocytes was performed to detect the effects of certain natural compounds on RWWs and four active compounds were found. Electroantennogram (EAG) recordings and a behavior test showed that RWWs exhibited a significant response to phenylacetaldehyde (PAA) and an EAG measurement of dsRNA-LoryOR20-treated RWWs revealed a significant decrease in response to PAA. Our results revealed an olfactory molecular mechanism for the recognition of PAA by RWWs, thus providing a potential genetic target at the peripheral olfactory sensing level, contributing to the development of novel control strategies for pest management.

研究领域: 昆虫学 · 分子生物学 · 农业科学

✓ 已验证 DOI PubMed 高影响因子

查看原文查看 PubMed

PEST MANAGEMENT SCIENCE · 2026 · IF 4.2

Response of Odorant Receptor LoryOR24 to (E,E)-2,4-Heptadienal and the Effects on the Behavior of the Rice Water Weevil Lissorhoptrus oryzophilus (Coleoptera: Curculionidae)

Xing-Yuan Wang,Xin-Xin Zhang,Xiao Wang,Yi-Fan Zhang,Jia-Rui Tao,Ju-Hong Zhang,Jun Wang,Shang Wang,Jing-Hui Xi

BACKGROUND Insect odorant receptors (ORs) play a crucial role in the peripheral olfactory system in detecting odorants. The ORs of Lissorhoptrus oryzophilus (RWW), namely LoryOR7, LoryOR20 and LoryOR27, have been identified, yet only LoryOR20 has been functionally characterized and shown to respond to phenylacetaldehyde (PAA). Most LoryORs remain to be further explored. RESULTS Quantitative real-time (qRT)-PCR revealed significant expression of LoryOR24 in the antennae. We used the Xenopus laevis oocyte expression system and two-electrode voltage clamp (TEVC) technique to express LoryOR24/LoryOrco to detect the effects of rice volatiles, and two rice volatiles were found to be active. As the concentration of ( E , E )-2,4-heptadienal gradually increased, the EAG response amplitude of RWWs also increased, peaking at 100 μg μL −1 (8.09 ± 0.61 mV). The behavioral assay confirmed that RWWs exhibited a significant response to 10 μg μL −1 ( E , E )-2,4-heptadienal, with an attraction rate of 77.51%. After silencing LoryOR24 via RNAi, RWWs showed significantly reduced electrophysiological and behavioral responses. Furthermore, molecular docking and site-directed mutagenesis identified that residue F133 is critical for the ligand binding and functional activation of LoryOR24. CONCLUSION The results of our study reveal the molecular basis underlying RWW's recognition of ( E , E )-2,4-heptadienal, providing a foundation for the discovery of potential genetic targets at the peripheral olfactory perception level. © 2026 Society of Chemical Industry.

✓ 已验证 DOI PubMed 高影响因子

查看原文查看 PubMed

查找使用阿拉丁产品的科研文献

与阿拉丁产品相关的已发表科研成果

热门产品

Response of Odorant Receptors with Phenylacetaldehyde and the Effects on the Behavior of the Rice Water Weevil (Lissorhoptrus oryzophilus)

Response of Odorant Receptor LoryOR24 to (E,E)-2,4-Heptadienal and the Effects on the Behavior of the Rice Water Weevil Lissorhoptrus oryzophilus (Coleoptera: Curculionidae)