Animal Nutrition
·
2024
·
IF 6.1
Menglin Shi,Haomin Li,Tianyu Chen,Bocheng Huang,Xiaoyue Li,Xiaohui Dong,Shuyan Chi,Qihui Yang,Hongyu Liu,Junming Deng,Beiping Tan,Shuang Zhang,Shiwei Xie
This experiment aimed to evaluate the impact of dietary hydroxyproline (Hyp) supplementation on the flesh quality of juvenile Pacific white shrimp ( Litopenaeus vannamei ) fed a low fishmeal diet. Six formulated diets included one high fishmeal (HF; 25% fishmeal content) and five low fishmeal diets (10% fishmeal content) with 0%, 0.2%, 0.4%, 0.6% and 0.8% Hyp (LF0, LF2, LF4, LF6 and LF8, respectively). Each diet was assigned to four replicates and 40 shrimp (0.32 ± 0.00 g) per replicate were fed four times a day for 8 weeks. Dietary Hyp supplementation had little effects on growth performance, but significantly increased the contents of Hyp, prolyl 4-hydroxylases (P4Hs), and collagen ( P < 0.05). The meat yield, springiness, hardness, chewiness, and cohesiveness of muscle were the highest in the LF2 group. Cooking loss and freezing loss of muscle were the lowest in the LF2 group ( P < 0.05). Dietary supplementation with 0.4% Hyp increased the myofiber density and decreased the myofiber diameter of muscle ( P < 0.05). Supplementation of Hyp in the diet up-regulated the mRNA expression of smyhc5 , smyhc15 , col1a1 , col1a2 , igf-1f , tgf-β and tor , and down-regulated the mRNA expression of smyhc 1 , smyhc 2 , smyhc 6a ( P < 0.05). Supplementation of Hyp in the diet up-regulated the protein expression of P-4E-BP1, P-AKT, AKT and P-AKT/AKT ( P < 0.05). These results suggested that the addition of 0.4% Hyp to low fishmeal diets improved the flesh quality of L. vannamei .
研究领域:
水产养殖
·
营养学
·
海洋生物学
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DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2022
·
IF 4.622
Feng Hu,Yan Wang,Jingjie Hu,Zhenmin Bao,Mengqiang Wang
Lysozyme is relevant to the innate immune system as a vital protein for crustaceans. In the present study, we cloned and characterized a novel c-type lysozyme gene ( Lv LYZ) from the Pacific white shrimp ( Litopenaeus vannamei ). The obtained full-length cDNA of Lv LYZ was 990 bp and contained an open reading frame of 693 bp. Its deduced amino acid sequence consisted of 230 amino acids (aa) with a 17 aa signal peptide at the N-terminal and 130 aa functional domains. The multiple sequence alignment (MSA) indicated that the typical active sites in Lv LYZ were similarly conserved as c-type lysozymes from other species. The transcription of Lv LYZ appeared in all detected tissues and had relatively higher expression levels in hemocytes , hepatopancreas , gill and intestine. The mRNA expression profiles of Lv LYZ were up-regulated in hemocyte and hepatopancreas post the stimulation of Vibrio parahaemolyticus or white spot syndrome virus (WSSV), respectively. The recombinant protein of Lv LYZ (r Lv LYZ) exhibited antibacterial activities against various microbes, including Escherichia coli , Vibrio splendidus , Micrococcaus luteus , Vibrio parahaemolyticus and Staphylococcus aureus . These results indicated that Lv LYZ could cope with bacteria in L. vannamei and may play a significant role in immune response against invading pathogens.
研究领域:
分子生物学
·
免疫学
·
水产养殖学
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产品关联
DOI
PubMed
高影响因子
AQUACULTURE
·
2025
·
IF 3.9
Letian Li,Yuan Wang,Laizhuang Jin,Lei Feng,Qianhui Song,Di Fang,Na Ying,Xincang Li,Wenhong Fang
The gastric sieve (GS) is a filtering structure found in the posterior stomach chamber (PSC) of Litopenaeus vannamei , which allows small particles to enter the hepatopancreas directly. However, it remains unclear if pathogens can also enter the hepatopancreas through this pathway. In this study, we examined the internal structural changes of the PSC via histological and ultra-structure analysis. We also orally administered fluorescent microspheres and bioparticles ( Vibrio parahaemolyticus and WSSV) of varying sizes to shrimp to determine their passage under hypoxia stress. The results showed that the PSC could be divided into four segments based on structural changes, and the GS performed its filtering function through two kinds of setae with different morphologies. Under normal dissolved oxygen levels (approximately 7.6 mg/L), V. parahaemolyticus and 1 μm fluorescent microspheres were unable to enter the hepatopancreas via GS. In contrast, under hypoxia stress (1.5–2 mg/L), both vibrio and 1 μm fluorescent microspheres could enter the hepatopancreas via GS in a short timeframe. WSSV and 0.1 μm fluorescent microspheres could enter the hepatopancreas via GS irrespective of hypoxia stress, while 5 μm fluorescent microspheres could not enter the hepatopancreas via GS even under hypoxia. These results suggest that GS functions as a particle diameter-dependent filter, with its filtering function influenced by hypoxia stress. Pathogens around 1 μm in size can invade the hepatopancreas through this pathway under hypoxia stress, providing novel insights into the mechanisms of pathogen invasion in shrimp.
CAS / MDL:
研究领域:
水产养殖学
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海洋生物学
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产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Qingfang Ying,Shengnan Zhan,Qiuyu Lu,Wenge Yang,Ru Jia,Gaoshang Li,Tao Huang
This study utilized κ-carrageenan (κC), konjac glucomannan (KGM), and xanthan gum (XG) to develop fish gelatin-anthocyanin intelligent hydrogels. The gelation properties, structural characteristic of hydrogels in monitoring shrimps (Litopenaeus vannamei) freshness were explored. The results showed that the addition of polysaccharides to fish gelatin (FG) notably enhanced gelation properties and color stability. During storage of shrimp at 4 °C, the intelligent hydrogels exhibited a progressive color change from transparent to brown. This color change demonstrated a strong linear relationships with total viable counts (TVC) and a significant nonlinear correlations with total volatile basic nitrogen (TVB-N) levels, indicating the intelligent hydrogels’ high reliability and potential for practical application. This approach offers a novel method for assessing and monitoring the quality of fresh aquatic products.
研究领域:
食品科学
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材料科学
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生物技术
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产品关联
DOI
高影响因子
AQUACULTURE INTERNATIONAL
·
2025
·Vol. 33
·期 7
·1-22
·
IF 2.4
Chen Zhengxu,Jing Futao,Zhang Tengwen,Yu Xin,Liao Liwen,Li Yaobing,Wen Yuxin,Pan Luqing
This study integrates network pharmacology, molecular docking, and a 56-day feeding trial to evaluate the effects of dietary pterostilbene (PTE) on growth, physiological status, and underlying regulatory pathways in Litopenaeus vannamei . The results indicate that dietary supplementation with a specific dose of PTE enhances shrimp growth, improves metabolic capacity, boosts antioxidant defenses, strengthens immune responses, and reduces inflammation. The regulatory mechanism of PTE involves targeting and binding to key proteins in lipid metabolism-related signaling pathways, including AKT , INSR , and SIRT1 within the insulin and SIRT1 - AMPK pathways. This binding significantly increases the gene expression levels of these targets ( P < 0.05), thereby activating the respective signaling pathways and enhancing glycolipid metabolism capacity. In terms of immune function, PTE targets and binds to key antioxidants and immune proteins, including Nrf2 , Relish , and Dorsal , resulting in a significant increase in the expression levels of these genes ( P < 0.05). Consequently, the expression of downstream antioxidant enzymes associated with Nrf2 ( SOD , CAT , GPX ) was significantly elevated ( P < 0.05), enhancing antioxidant capacity. Additionally, the expression of antibacterial lysosomal factors ( PEN3 , CRU , ALF , and LYC ) also increased ( P < 0.05), thereby strengthening immune function. Moreover, dietary PTE reshapes the structure of gut microbiota and increases the abundance of beneficial bacteria. This study elucidates the molecular mechanisms and optimal dosage of dietary PTE in improving the physiological function of shrimp by combining molecular docking with in vivo findings, providing a theoretical basis for the development of efficient and safe aquaculture feed additives.
研究领域:
水产养殖
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分子生物学
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营养生理学
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免疫学
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生物信息学
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药理学
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产品关联
DOI
高影响因子
Journal of Marine Science and Engineering
·
2021
·Vol. 9
·期 6
·677
·
IF 2.458
Kangxuan Chen,Shihao Li,Jianhai Xiang,Amir Sagi,Fuhua Li
The insulin-like androgenic gland hormone (IAG) plays a key role in male sexual differentiation and spermatogenesis in crustaceans. The expression of IAG is usually negatively regulated by neuropeptide hormones through the “eyestalk-AG” endocrine axis. However, the underlying mechanism is still largely unknown. In the present study, we performed a comparative transcriptome analysis on the androgenic gland (AG), the main secretory organ of IAG, ofL. vannameibefore and after unilateral eyestalk ablation. A total of 67 differentially expressed genes (DEGs) were identified, including some putative genes involved in sexual development. Interestingly, several genes related to molting and endocrine processes were found differentially expressed between the two treatments. Further investigation on the expression profiles of these genes andLv-IAGshowed that their expression patterns were closely correlated throughout the molting cycle. In addition, injection of 20-hydroxyecdysone (20E) could inhibit the expression ofLv-IAGat different molting stages in a dosage-dependent manner. The data indicated that ecdysteroids played important roles in regulating theLv-IAGexpression and might bridge the endocrine axis between eyestalk and AG. The present study provided new insights into understanding the regulation of sexual development in male crustacean.Keywords:insulin-like androgenic gland hormone;sexual development;endocrine axis;ecdysteroids;crustacean
研究领域:
内分泌学
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分子生物学
·
发育生物学
·
水产养殖
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DOI
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2022
·
IF 9.336
Na Li,Ke-Xin Zhang,Jia-Yi Du,Zhi-Feng Tan,Yun-Peng Xu,Xiao-Yang Liu,Da-Yong Zhou,De-Yang Li
The effects of high-intensity ultrasound on the physicochemical and gelling properties of Litopenaeus vannamei ( L. vannamei ) myofibrillar protein (MP) were investigated. MP solutions were subjected to ultrasound treatment (power 100 W, 300 W, and 500 W). It was found that the carbonyl and free amino contents of MP increased significantly with increasing ultrasound power, accompanied by enhanced emulsification properties. The increase of free radical and carbonyl content indicated that ultrasound induced the oxidation of MP. With the increase of ultrasound power, it was found that the total sulfhydryl content of the shrimp MP decreased, but the surface hydrophobicity increased significantly, which might be closely related to the conformational changes of MP. Meanwhile, a significant increase of β-sheet but a decrease of α-helix in the secondary structure of MP was observed with increasing ultrasound power, indicating that ultrasound treatment induced the stretching and flexibility of MP molecules. SDS-PAGE showed that L. vannamei MP consisted of myosin heavy chain, actin, myosin light chain, paramyosin and tropomyosin. Ultrasound treatment could lead to some degree of oxidative aggregation of MP. The results of rheological properties indicated that ultrasound treatment enhanced the viscoelasticity of MP and further improved the gel strength of MP gel. This study can provide a theoretical basis for the functional modification of shrimp MP and the processing of its surimi products.
研究领域:
食品科学
·
蛋白质化学
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产品关联
DOI
PubMed
高影响因子
MARINE POLLUTION BULLETIN
·
2020
·
IF 4.049
Lefei Jiao,Tianmeng Dai,Tinglan Cao,Min Jin,Peng Sun,Qicun Zhou
Heavy metal pollution arising from agricultural and industrial activities poses a significant threat to the aquatic environment, especially the increasing levels of chromium (Cr) that is exacerbating marine pollution. Given the economic importance of the Pacific white shrimp Litopenaeus vannamei ( L. vannamei ), understanding the impact of marine Cr pollution is deemed to be significant. In this study, we used the transcriptome sequencing (RNA-seq) technique to characterize the molecular mechanism of Cr exposure in L. vannamei . Gene ontology enrichment analysis showed substrate-specific and ion transport-related functions were mainly influenced by Cr exposure. We further identified genes involved in protein digestion and absorption (PEPT1, BAT1, MDU1), chemical carcinogenesis (GST and UGTs), ABC transporters (ABCC2), apoptosis (CAPN1, CASP10, PARP), implying the potentially Cr disintoxication mechanisms in L. vannamei . Genes within pancreatic secretion (ALT, LDH), lysosome (CTSL and HEXB), and peroxisome (ACOX1, ECI2, NUDT12) pathways implied the potentially Cr toxicity mechanisms in L. vannamei .
研究领域:
环境科学
·
分子生物学
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DOI
PubMed
高影响因子
Journal of Future Foods
·
2025
·
IF 7.2
Xin Zhao,Shunliang Zhang,Xiaoyu Cheng,Bing Zhao,Shouwei Wang,Yan Zhao,Le Wang
Pretreatment methods and drying process play vital role in the quality and flavor changes of shrimp. The volatile and taste profiles in high pressure processing (HHP at 300MPa-5min, 300MPa-10min, 500MPa-5min, and 500MPa-10min) pretreatment shrimps after hot-air drying process were investigated by using the electronic tongue, electronic nose, high performance liquid chromatography, and gas chromatography/mass spectrometry. The results showed that HHP pretreatment endowed shrimps with better volatile and taste attributes to varying degrees, which significantly ( P < 0.05 ) increased total umami free amino acids (1.18−2.16 times), total sweet free amino acid (1.09−1.27 times) contents ( P < 0.05), promoted the production of pleasant taste nucleotides (1.52−1.83 times) and organic acids (1.19−1.78 times), and produced more volatile components (1.10−1.46 times) when compared to untreated group. Glycine, alanine, valine, lysine, histidine, arginine, guanosine monophosphate, inosine monophosphate, and succinic acid were main non-volatile active components. The equivalent umami values is: 500MPa-5min > 500MPa-10min > 300MPa-10min > 300MPa-5min > control group. Compared with control group, HHP pretreatment endowed shrimps with richer aldehydes, ketones, alcohols and pyrazine compounds, while control endowed shrimps with richer hydrocarbons. Based on these flavor profiles, the principal component analysis of E-tongue and E-nose data showed a distinct difference among the different pretreatment group. Generally, HHP at 500 MPa-5min was beneficial for improving EUC and volatile compounds of dried shrimps. The findings may provide theoretical reference for the development of high-quality drying technology for shrimps.
研究领域:
食品科学
·
食品加工
·
风味化学
·
水产制品
·
分析化学
·
感官分析
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产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
·
IF 6.2
Xianbin Su,Teng Li,Xiaowen Zhu,Peihua Zheng,Huakang Pan,Hui Guo
Nonylphenol (NP) is one of the common pollutants in the environment that have toxic effects on aquatic animals. Nevertheless, little is known about the possible toxicity mechanism of NP on the hepatopancreas of Litopenaeus vannamei . In the present study, the detrimental effects of NP on the hepatopancreas of the L. vannamei were explored at the histological and transcriptomic levels. The findings indicated that after NP exposed for 3, 12, and 48 h, the hepatopancreas histology was changed significantly. Transcriptomic analysis showed that a total of 4302, 3651, and 4830 differentially expressed genes (DEGs) were identified at 3, 12, and 48 h following NP exposure. All these DEGs were classified into 12 clusters according to the expression patterns at different time points. GO and KEGG enrichment analyses of DEGs were also performed, immunological, metabolic, and inflammatory related pathways, including arachidonic acid metabolism (ko00590), the PPAR signaling pathway (ko03320), and the regulation of TRP channels by inflammatory mediators (ko04750) were significantly enriched. Six DEGs were selected for validation by quantitative real-time PCR (qRT-PCR) and the results confirmed the reliability of transcriptome data. All results indicated that NP is toxic to L. vannamei by damaging the histopathological structure and disrupting the biological function. The findings would provide a theoretical framework for lowering or limiting the detrimental impacts of NP on aquaculture and help us to further study the molecular toxicity of NP in crustaceans.
研究领域:
水产养殖学
·
环境毒理学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Jie Feng,Min Hu,Chuandong Fang,Soottawat Benjakul,Huimin Lin,Bin Zhang
Modified atmosphere packaging (MAP) is a widely used technique for preserving aquatic products, but research on the application of hydrogen (H 2 ) in seafood preservation remains limited. This study evaluated the effects of two MAP (MAP1: 50% carbon dioxide (CO 2 )/50% N 2 ; MAP2: 75% CO 2 /25% N 2 ) and two H 2 - reducing atmosphere packaging (RAP, H 2 -RAP1: 0.5% H 2 /50% CO 2 /49.5% N 2 ; H 2 -RAP2: 0.5% H 2 /75% CO 2 /24.5% N 2 ) on protein degradation and microbial communities in Litopenaeus vannamei stored 4 °C. Protein degradation was assessed by trichloroacetic acid (TCA)-soluble peptides, myofibrillar protein (MP), free amino acids (FAA), and biogenic amines (BA), while microbial dynamics were analyzed using total viable count (TVC) and 16S rRNA high-throughput sequencing. Results showed that the H 2 -RAP2 group exhibited the best preservation effect. After 12 d, MP content in the H 2 -RAP2 group remained at 63.23 mg/g, significantly higher than in the other groups. The H 2 -RAP2 group also effectively delayed the accumulation of TCA-soluble peptides, FAA, and BA (mainly histamine, putrescine, tyramine, and cadaverine), while markedly suppressing TVC growth (5.91 log CFU/g). This study confirms the synergistic preservation effect of H 2 and CO 2 , supporting the application of H 2 -RAP preservation of Litopenaeus vannamei and other aquatic products.
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DOI
PubMed
高影响因子
Viruses-Basel
·
2022
·Vol. 14
·期 12
·2731
·
IF 5.818
Xu Zhang,Li-Peng Shan,Qi Zhao,Lei Liu,Xu OuYang,Yang Hu,Chen-Jie Fei,Jiong Chen
An outbreak of white spot syndrome virus (WSSV) can hit shrimp culture with a devastating blow, and there are no suitable measures to prevent infection with the virus. In this study, the activity of active molecules from Chinese herbs against WSSV was evaluated and screened. Taxifolin had the highest rate (84%) of inhibition of the WSSV infection. The viral infectivity and genome copy number were reduced by 41% when WSSV virion was pretreated with taxifolin prior to shrimp infection. A continuous exchange of taxifolin significantly reduced the mortality of shrimp infected with WSSV. Due to the WSSV virion infectivity being affected by taxifolin, the horizontal transmission of the virus was blocked with an inhibition rate of up to 30%, which would further reduce the cost of a viral outbreak. Additionally, the viral genome copy number was also reduced by up to 63% in shrimp preincubated in taxifolin for 8 h. There may be a connection to the enhancement of innate immunity in shrimp that resulted in a 15% reduction in mortality for taxifolin-fed shrimp after the WSSV challenge. After dietary supplementation with taxifolin, the resistance of larvae to WSSV was improved, indicating that taxifolin may be a potential immunostimulant for shrimp to prevent WSD. Therefore, the results indicate that taxifolin has application potential for blocking a WSSV outbreak and reducing the loss of shrimp culture.Keywords:taxifolin;WSSV;antiviral;Chinese herbs;Litopenaeus vannamei
研究领域:
海洋生物学
·
免疫学
·
病毒学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）