Journal of Materials Chemistry B
·
2019
·Vol. 8
·期 16
·3542-3549
·
IF 5.047
Mohamed Ibrahim Halawa,Fengxia Wu,Muhammad Nadeem Zafar,Islam M. Mostafa,Abubakar Abdussalam,Shuang Han,Guobao Xu
In this work, a glutathione (GSH) sensing nano-platform using lucigenin as a fluorescent probe in the presence of MnO2 nanosheets was reported for the first time. Unlike the earlier fluorescent detection systems based on MnO2 nanosheets, which depend on Förster resonance energy transfer (FRET) or the dynamic quenching effect (DQE), the mechanism of the quenching process of MnO2 nanosheets on lucigenin fluorescence was attributed mainly to a static quenching effect (SQE) with a minor contribution of the inner filter effect (IFE). A double exponential fluorescence decay of lucigenin was obtained in various MnO2 nanosheet concentrations as a result of their SQE and IFE. Based on this phenomenon and taking advantage of the redox reaction between GSH and MnO2 nanosheets, we have developed a switch-on sensitive fluorescent method for GSH via the recovery of the MnO2 nanosheet-quenched fluorescence of lucigenin. A good linearity range of 1.0–150.0 μM with a low limit of detection (S/N = 3) of 180.0 nM was achieved, revealing the higher sensitivity for GSH determination in comparison with the previously reported MnO2 nanosheet-based turn-on fluorescent methods. The developed fluorescent nano-platform exhibits excellent selectivity with successful application for GSH detection in human serum plasma, indicating its good practicability for GSH sensing in biological and clinical applications.
研究领域:
分析化学
·
生物医学工程
·
纳米技术
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PubMed
高影响因子
LUMINESCENCE
·
2015
·Vol. 30
·期 7
·990-995
·
IF 1.518
Quan Chi,Wanying Chen,Zhike He
The chemiluminescence (CL) of lucigenin (Luc 2+ ) can be enhanced by different alcohols in alkaline solution. The effect of different fatty alcohols on the CL of lucigenin was related to the carbon chain length and the number of hydroxyl groups. Glycerol provides the greatest enhancement. UV/Vis absorption spectra and fluorescence spectra showed that N -methylacridone (NMA) was produced in the CL reaction in the presence of different alcohols. The peak of the CL spectrum was located at 470 nm in all cases, indicating that the luminophore was always the excited-state NMA. The quenching of lucigenin CL by superoxide dismutase (SOD) and the electron spin resonance (ESR) results with the spin trap of 5,5-dimethyl-1-pyrroline N -oxide (DMPO) demonstrated that superoxide anions (O 2 •– ) were generated from dissolved oxygen in the CL reaction and that glycerol and dihydroxyacetone (DHA) can promote O 2 •− production by the reduction of dissolved oxygen in alkaline solution. It was assumed that the enhancement provided by different alcohols was related to the solvent effect and reducing capacity. Glycerol and DHA can also reduce Luc 2+ into lucigenin cation radicals (Luc •+ ), which react with O 2 •− to produce CL, and glycerol can slowly transform into DHA, which is oxidized quickly in alkaline solution. Copyright © 2015 John Wiley & Sons, Ltd.
研究领域:
生物化学
·
分析化学
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Xiangui Ma,Wenyue Gao,Mohamed Ibrahim Halawa,Yixiang Lan,Jianping Li,Guobao Xu
Tyrosinase is a key enzyme in melanin biosynthesis and its serum level is an important biomarker for some dermatoses such as vitiligo and melanoma. Thus, a sensitive and facile assay for tyrosinase activity is in urgent demands. Lucigenin is a well-known chemiluminescent luminophore, but has been rarely been employed for fluorescence detection. In this work, a facile method to detect tyrosinase activity based on lucigenin fluorescence has been reported for the first time. When tyrosinase was mixed with tyrosine, tyrosine was oxidized to generate melanin-like polymers which can effectively quench the fluorescence of lucigenin. A linear range of tyrosinase from 0.25 to 25 μg/mL was obtained with detection limit of 0.2 μg/mL. In addition, a useful platform for screening potential tyrosinase inhibitors was constructed based on the proposed system. The linear range is from 2.5 to 62.5 μg/mL and the detection limit is as low as 1.0 μg/mL for kojic acid , a typical tyrosinase inhibitor.
研究领域:
生物化学
·
医学检测
·
皮肤病学
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高影响因子
JOURNAL OF FOOD AND DRUG ANALYSIS
·
2022
·
IF 6.157
Shuai Quan,Kaixiang Ji,Fangshuo Liu,Tesfaye Hailemariam Barkae,Mohamed Ibrahim Halawa,Saima Hanif,Baohua Lou,Jianping Li,Guobao Xu
Tetracycline was reported to trigger intense lucigenin chemiluminescence (CL). Based on its significant quenching to lucigenin-tetracycline CL, procyanidin was detected. The mechanism of the lucigenin-tetracycline CL and its quenching by procyanidin were proposed. Under the optimum conditions, the calibration curve is linear in the range of 10–1000 nM for procyanidin with a detection limit of 4.4 nM. The RSD for nine replicate determinations of procyanidin was less than 1.7%. Moreover, this method was satisfactorily applied to the determination of procyanidin in human urine samples and health food capsule of grape seed.
研究领域:
分析化学
·
药物分析
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高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Yixiang Lan,Fan Yuan,Tadesse Haile Fereja,Chao Wang,Baohua Lou,Jianping Li,Guobao Xu
Lucigenin-riboflavin chemiluminescence is reported for the first time. Moreover, most dopamine chemiluminescence (CL) detection methods are based on the quenching of CL by dopamine. In contrast, we find that dopamine can significantly enhance lucigenin/riboflavin CL and establish a highly sensitive turn-on method for dopamine detection based on the enhancement of lucigenin/riboflavin CL. Under the optimal conditions, the CL intensity of the lucigenin/riboflavin system increased linearly with the concentration of dopamine in the range of 0.0056–55.56 μM, and the limit of detection is 1.87 nM.
研究领域:
分析化学
·
生物化学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2026
·
IF 6.2
Fugang Li,Qiang Li,Feng Chen,Chenxu Yan,Yongxi Zhao,Zhiqian Guo
High sensitivity and accuracy in detecting the myocardial damage biomarker, human cardiac troponin T (cTnT), is critical for the early diagnosis of acute myocardial infarction. However, conventional chemiluminescent (CL) probes suffer from instability, poor solubility, and inadequate bioavailability, which limit their clinical utility. Here, we describe a novel nanosized CL system (lucigenin@PS-COOH) for cTnT detection, wherein carboxyl polystyrene (PS-COOH) microspheres encapsulate lucigenin, significantly enhancing stability, water dispersibility, and antibody conjugation. Compared with traditional CL probes, lucigenin@PS-COOH exhibits exceptional stability, biocompatibility, and CL signal intensity. In addition, lucigenin@PS-COOH demonstrates excellent linearity with clinical data from Longhua Hospital, suggesting strong potential as a clinical assay for cTnT detection in real-world patient samples. Thus, lucigenin@PS-COOH can serve as a powerful tool for accurate cTnT detection in clinical settings and facilitate further research into the role of cTnT in disease.
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PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2016
·
IF 5.886
Wenyue Gao,Zhongyuan Liu,Liming Qi,Jianping Lai,Shimeles Addisu Kitte,Guobao Xu
Glutathione (GSH) is a crucial antioxidant produced endogenously and plays key roles in biological systems. It is vitally important to design simple, selective, and sensitive methods to sense GSH and monitor changes of GSH concentration. In this work, the cathodic electrochemiluminescence (ECL) of lucigenin in the presence of MnO2 nanosheets at a glassy carbon electrode was utilized for GSH detection. GSH can reduce MnO2 nanosheets into Mn2+ which can obviously inhibit the ECL of lucigenin. The ECL inhibition efficiencies increase linearly with the concentrations of glutathione in the range of 10 to 2000 nM. The detection limit for GSH measurement is 3.7 nM. This proposed method is highly sensitive, selective, simple, fast, and cost-effective. Moreover, this approach can detect GSH in human serum samples with excellent recoveries, which indicates its promising application under physiological conditions.
研究领域:
分析化学
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生物医学工程
·
生物化学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
·
IF 4.831
Kaixiang Ji,Fangshuo Liu,Tesfaye Hailemariam Barkae,Shuai Quan,Abdallah M. Zeid,Wei Zhang,Jianping Li,Guobao Xu
N -hydroxyphthalimide (NHPI) is an efficient organic catalyst and an important chemical raw material which can be used as an intermediate in organic synthesis of drugs and pesticides. In this study, NHPI has been used as a coreactant of lucigenin chemiluminescence (CL) for the first time. The CL of the developed system is significantly enhanced in the presence of Co 2+ . Therefore, we developed a novel lucigenin-NHPI CL method coupled with flow injection analysis for the sensitive, precise, and selective determination of Co 2+ . The linear range of this method is 1–1000 nM, and the detection limit is 67 pM (S/N = 3). In addition, this method has a good selectivity for Co 2+ . It has been applied to the detection of Co 2+ in lake water, and the standard recovery rate is 95.9–103.2%, indicating that the method is feasible.
研究领域:
分析化学
·
环境科学
·
化工
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高影响因子
ANALYST
·
2026
·
IF 3.6
Runqi Yao,Jixing Huang,Zelin Gao,Hengbiao Zhang,Li Yang,Dekui Zhang,Tuanjie Che,Yuchao Li,Honghan Dong,Xusheng Xia,Ning Wang,Liyun Ding
Detecting the concentration of chloride ions in water and soil is of great significance for understanding chloride behavior in the ocean and monitoring environmental pollutants. A novel fluorescent chloride ion optical fiber sensor has been developed based on UiO-66-lucigenin complex, in which UiO-66 enhances the photostability of the fluorescent probe lucigenin and facilitates the adsorption of chloride ions. UiO-66-lucigenin complex were encapsulated in cellulose acetate through the physical embedding method to form a chloride ion sensitive fluorescence probe. This portable optical fiber sensor is capable of detecting chloride ions in both water and soil environments simultaneously with high sensitivity, excellent stability, rapid response time and resistance to ion interference. These features make it a promising tool for chloride ion detection in complex environmental conditions. Looking ahead, this sensor holds broad application prospects for detecting chloride ions in complex environments.
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Wenyue Gao,Liming Qi,Zhongyuan Liu,Saadat Majeed,Shimeles Addisu Kitte,Guobao Xu
Thiourea dioxide, a well-known eco-friendly, stable and cost-effective industrial reductant, has been used as the coreactant of lucigenin chemiluminescence for the first time. This chemiluminescence system is highly efficient, and its chemiluminescence peak intensity is about 75 times higher than that of the famous lucigenin/H 2 O 2 system. Interestingly, dopamine dramatically suppresses the chemiluminescence of lucigenin/thiourea dioxide system. Based on this newly-developed system, highly sensitive detection of dopamine, lucigenin, and thiourea dioxide was achieved. The linear ranges are 20–800 nM, 20 nM–0.1 mM, and 0.01–10 mM for dopamine, lucigenin, and thiourea dioxide, respectively. The detection limits are 14.7 nM, 8.0 nM, and 2.4 μM for dopamine, lucigenin, and thiourea dioxide, respectively. Moreover, this method shows excellent selectivity for the detection of dopamine against many compounds, such as ascorbic acid, uric acid, amino acids and sugars. This study suggests that the newly-found user-friendly lucigenin/thiourea dioxide system is a promising chemiluminescence system with broad applications.
研究领域:
分析化学
·
生物医学工程
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DOI
高影响因子
DYES AND PIGMENTS
·
2024
·
IF 4.1
Di Wu,Yi Wang,Wenjing Qi,Jianyang Xu,Kanglin Pei,Lingyan Ma,Lin Qi
Long-lasting chemiluminescence of lucigenin/p-aminophenol (4-AP) system is explored for the first time and possible chemiluminescence enhancement mechanism is also proposed in this work. 4-AP can enhance chemiluminescence of lucigenin nearly 150 times and is stable for nearly 5 min, which overcomes the need of flow syringe pump. While o-aminophenol (2-AP) slightly enhances chemiluminescence of lucigenin and m-aminophenol (3-AP) does not. Thus, it can be applied in discrimination of three aminophenol isomers. More importantly, cyclic voltammetry proves the lowest oxidation peak for 4-AP at 115 mV, which is speculated to be the main reason for 4-AP induced chemiluminescence enhancement. It has been applied in chemiluminescence enhancement detection of 4-AP and p-nitrophenol (4-NP) from 80 nM to 60 μM, from 0.5 μM to 60 μM with limit of detection (LOD) of 26 nM and 0.17 μM, respectively. It shows high selectivity and anti-interference in chemiluminescence enhancment detection of 4-AP and 4-NP towards other organic compounds and metal ions. It also exhibits high accuracy in chemiluminescence enhancment detection of 4-AP and 4-NP in lake water with recoveries from 98.7% to 101.5% for and recoveries from 98.7% to 102.2%, respectively. It expands the study of long-lasting lucigenin chemiluminescence system and chemiluminescence method utilized in discrimination of aminophenols isomers.
研究领域:
分析化学
·
环境科学
·
电化学
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高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.1
Jing Wang,Fuge Chai,Mengya Fan,Yongping Dong
The present work focuses on developing a highly sensitive ECL sensor for Pb 2+ detection based on WS 2 quantum dots (QDs). A top-down sonication-assisted liquid-phase exfoliation method was employed to synthesize WS 2 QDs which were characterized by TEM, XPS, Raman spectroscopy and electrochemistry. It was found that WS 2 QDs could not only generate mild cathodic ECL itself but also significantly promote the cathodic ECL of lucigenin. The electrochemical results suggested that WS 2 QDs could effectively accelerate the reduction process of dissolved oxygen and lucigenin, thereby greatly amplifying the ECL intensity. The specific interaction between Pb 2+ and WS 2 QDs could significantly decrease ECL signal due to the depletion of WS 2 QDs, based on which an ECL sensor for detecting Pb 2+ was developed. Under the optimal condition, a good linear relationship between the inhibited ECL signal and the logarithm of Pb 2+ concentration was obtained in the range from 1 nM to 50 μM, with a low detection limit of 10.4 pM. Moreover, the fabricated sensor exhibited high sensitivity and excellent selectivity, and could be used for the sensitive detection of Pb 2+ in real samples.
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高影响因子
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