FOOD HYDROCOLLOIDS
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2019
·
IF 5.839
Jingrong Liu,Hualin Wang,Min Guo,Linlin Li,Minmin Chen,Suwei Jiang,Xingjiang Li,Shaotong Jiang
This study was to design a novel wide pH–sensing colorimetric film of κ-carrageenan incorporated with extract from Lycium ruthenicum Murr. (Car-LRM) for food freshness monitoring. The structure, basic properties (thermal stability, mechanical, barrier and antioxidant activity), and release kinetics of LRM from the films were investigated. Attentions were centered on colorimetric response of films and their applications for freshness monitoring of milk and shrimps. A low incorporation of LRM (no more than 2.5%) led to an enhancement in thermal stability and water vapor barrier of films to a certain extent. At above level of incorporation, LRM could be well-distributed in Car matrix by the interactions of hydrogen bonds. In addition to good antioxidant activity , the film displayed colorimetric response at a wide range of pH (2−10) and the color change was reversible. The release of LRM from films followed Fickian's law which was controlled by diffusion mechanism rather than swelling mechanism. The successful applications of Car-LRM2.5 for the freshness monitoring of milk and shrimps indicate that Car-LRM2.5 may have great potential for monitoring the freshness of milk and aquatic products.
研究领域:
食品科学
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材料科学
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产品关联
DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2020
·Vol. 85
·期 3
·696-706
·
IF 2.479
Meilun Shen,Kang Liu,Yefei Liang,Guangxin Liu,Jie Sang,Cuiqin Li
The aim of this study was to extract and purify anthocyanins from Lycium ruthenicum Murr. and evaluate their tyrosinase inhibitory activity. Response surface methodology was devoted to optimize enzyme-assisted extraction of anthocyanins from L. ruthenicum dried fruits. Extraction at 38 °C for 37 min using water-containing pectinase (52.04 mg/100 g dried fruit) rendered an anthocyanin extraction yield of 19.51 ± 0.21 mg/g. The purified anthocyanins were separated from the extract by macroporous resin XDA-6. Antioxidant tests in vitro suggested that the extract and the purified anthocyanins exhibited a potent 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity, 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging capacity, hydroxyl radical scavenging capacity, superoxide radical scavenging capacity, and total reducing power. Thirteen anthocyanins from L. ruthenicum dried fruits were analyzed by HPLC-MS. Moreover, the purified anthocyanins had inhibitory effect on tyrosinase monophenolase (IC 50 = 1.483 ± 0.058 mg/mL), and the type of inhibition was competitive inhibition ( K i = 39.83 ± 1.4 mg/mL). The maximum inhibitory activity of the purified anthocyanins (3.00 mg/mL) on tyrosinase diphenolase was 42.16 ± 0.77%, and the type of inhibition was anticompetitive inhibition ( K is = 2.387 ± 0.10 mg/mL). Practical Application The anthocyanins from L. ruthenicum dried fruits can be used as tyrosinase inhibitors in medicine, cosmetics, and food preservation industries.
研究领域:
药理学
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食品科学
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生物化学
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植物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
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IF 5.895
Yi-Kao Hu,Xiao-Lin Bai,Hao Yuan,Yi Zhang,Emmanuel Ayodeji Ayeni,Xun Liao
The fruits ofLycium ruthenicum Murr have long been consumed as health food and used in folk medicine in China. Apart from the well-known polysaccharides, the active small molecular constituents in this fruit have not been fully studied. In this work, a systematic phytochemical study was carried out to investigate the small molecules and their potential health benefits. Nine new polyphenolic glycosides, lyciumserin A–I (1–9), together with 16 known compounds (10–25), were isolated and elucidated by high-resolution electrospray ionization mass spectrometry and comprehensive NMR analyses in combination with chemical hydrolysis. Compounds 1, 2, and 16 exhibited moderate inhibitory activity of monoamine oxidase B (MAO-B), while compounds 1 (50 μM) and 2 (100 μM) displayed significant neuroprotective effects (69.22 and 72.38% of cell viability, respectively) in the 6-hydroxydopamine-induced injury of the PC12 cell model (54.41%), comparable to the positive drug rasagiline (70.45%). The neuroprotective effect of 1 and 2 was further evidenced by the observation of the morphological change and fluorescein diacetate/propidium iodide staining. In addition, the levels of the major active compounds (1, 3, 5/6, and 16–18) vary from 21.5 to 892.3 μg/g. This is the first report on phenolic glycosides from the fruits ofL. ruthenicum Murr that possess both significant MAO-B inhibitory and neuroprotective effects, indicating the promising potential of the fruits for the development of health care products and even therapeutic agents for the treatment of Parkinson’s disease and other neurodegenerative diseases.
研究领域:
药理学
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神经科学
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植物化学
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天然产物
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产品关联
DOI
PubMed
高影响因子
Frontiers in Sustainable Food Systems
·
2022
·
IF 5.005
Dong Yuhe,Zhong Wenting,Yang Chunmiao,Zhang Yongze,Yang Dongsheng
The objectives of this study are to optimize the ultrasonic microwave synergistic extraction (UMSE) of anthocyanins from Lycium ruthenicum Murr (ALRM) by response surface methodology and also to investigate its antioxidant activity in vitro. Based on the single-factor experiments, we optimized the ALRM extraction process by response surface methodology assuming anthocyanin extraction rate as the response point and microwave power, ultrasonic power, extraction time, and liquid-to-material ratio as the investigating parameters as well as performed correlation analysis between DPPH·, ·OH, ABTS·, O2-· radical scavenging potential, and ferric-reducing antioxidant power. The results showed that the influence on anthocyanin yield was in the order of liquid to material ratio > microwave power > ultrasonic power > extraction time; the elicited extraction conditions were: ultrasonic power 216.253 W, microwave power 89.311 W, time 26.141 min, liquid to material ratio 17.294 mL/g, the forecast content was 10.157 mg/g, while the actual value was consistent with the preview value. Meanwhile, ALRM obtainable by UMSE was identified as yielding favorable antioxidant properties firstly, when the concentration of purified ALRM (ALRM-1) was increased, the antioxidant capacity was strengthened. Theoretically, we provide a basis for the extraction procedure of ALRM and its antioxidant activity, which serves as a promising antioxidant and free radical scavenger.
研究领域:
食品科学
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植物化学
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分析化学
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营养学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
·
IF 6.2
Yi-Kao Hu,Ayzohra Ablat,Xiao-Lin Bai,Xiang Yin,Chao Ma,Xun Liao
Lycium ruthenicum Murr is an important edible and medicinal plant widely cultivated in Northwest China. Our previous study found that its fruit possessed rich polyphenols exhibiting significant neuroprotective effects, inspiring us to investigate the active constituents of its rootstocks, which have never been reported. In this study, we rapidly identified four phenolamides (1–4) possessing monoamine oxidase B (MAO-B) inhibitory effect by using MAO-B-functionalized cellulose filter paper-based ligand fishing, and their structures were elucidated with NMR and MS. Compounds 1, 2, and 4 exhibited moderate inhibitory effect on MAO-B with IC50 values of 71.44 ± 1.81, 28.97 ± 1.20, and 25.58 ± 0.74 μM, respectively, and enzyme kinetic analysis, molecular docking, and molecular dynamics further revealed the inhibition mechanism. All four phenolamides displayed a neuroprotective effect against 1-methyl-4-phenylpyridinium -induced SH-SY5Y cells model, among which 2 and 4 significantly reduced intracellular MAO-B activity and improved the ATP content. Western blot revealed that compounds 2 and 4 alleviated the oxidative stress through up-regulation of the expression of Nrf2 and HO-1 protein, promoting mitophagy via activating the PINK1/Parkin pathway. Moreover, compounds 2 and 4 extended the lifespan, dopamine and ATP levels, locomotor behavior, and olfactory ability of the PINK1B9 flies, a drosophila model of Parkinson’s disease. Meanwhile, compounds 1–4 showed potent antioxidative activities on DPPH, ABTS+, and PTIO radical scavenging assays. These results revealed the health-promoting effects of the rootstocks in preventing and treating neurodegenerative diseases, laying the scientific foundation for the exploitation of this plant byproduct for the development of functional food formulations and pharmaceutical products.
研究领域:
植物化学
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神经科学
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药理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2018
·Vol. 83
·期 10
·2448-2456
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IF 2.018
Xiaohui Zhao,Banmacailang Dong,Pi Li,Wei Wei,Jun Dang,Zenggeng Liu,Yanduo Tao,Hongping Han,Yun Shao,Huilan Yue
Lycium ruthenicum Murr. (LRM, Solanaceae), also called “black wolfberry” in China, is extremely tolerant drought. Although black wolfberry has seeds that are rich in oil, being considered as a potential alternative for edible oil production, little information has been found regarding its characterization. The present study evaluated the fatty acid profile of the seed oil and its phytosterol contents. Moreover, its antioxidant potential and its inhibition activities on pancreatic lipase, and cholesterol esterase (CEase) were also determined. The key fatty acids of studied oil were linoleic (74.58%), oleic (11.82%), γ -linolenic (6.60%), palmitic (4.88%), and stearic (1.40%) acids, which together comprised 99.28% of the total fatty acids. Phytosterols including 24-methylenecholesterol, campesterol, stigmasterol, dihydrolanosterol, 24-methyldesmosterol, β -sitosterol, Δ 5 -avenasterol, cycloartenol, and Δ 7− avenasterol were identified for the 1st time. The inhibition values (IC 50 ) for the DPPH and ABTS radical scavenging capacities of the oil were 7.86 and 15.49 mg/mL, respectively. Additionally, LRM seed oil was also demonstrated to possess strong pancreatic lipase, and CEase inhibition activities with IC 50 values of 12.38 and 2.63 mg/mL, respectively. The mechanism study indicated that the inhibitory manner of the oil on CEase belonged to uncompetitive inhibition of reversible inhibition. The results derived from above studies suggest that LRM seed oil could be a potential source of high value phytochemicals for developing novel functional food.
研究领域:
食品科学
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植物化学
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营养学
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DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Shihai Zhang,Fei He,Xia Chen,Kan Ding
Crude polysaccharides were obtained from fruits of Lycium ruthenicum Murr using hot water extraction followed by ethanol precipitation. A homogeneous polysaccharide, LRP3-S1 with a relative molecular weight of 114.8 kDa was purified by anion-exchange chromatography on DEAE Sepharose ™ Fast Flow and Sephacryl S-300 HR column. Monosaccharide composition analysis revealed that LRP3-S1 was composed of rhamnose, galacturonic acid, galactose, xylose and arabinose in a molar ratio of 14.4: 17.7: 26.6: 16.4: 24.9. LRP3-S1 contained a rhamnogalacturonan I (RG-I) backbone partially substituted at C-4 of rhamnose units by side chains, which included T-linked β-D-Gal p , 1,3-linked β-D-Gal p , 1,6-linked β-D-Gal p , 1,3,6-linked β-D-Gal p , 1,5-linked α-L-Ara f , 1,3,5-linked α-L-Ara f , T-linked α-L-Ara f and T-linked β-D-Xyl p . Biological activity tests showed that LRP3-S1 could inhibit the growth of pancreatic cancer cells. In addition, LRP3-S1 could attenuate invasion ability of BxPC-3 cells and down-regulate protein expression of p-FAK, p-AKT, p-GSK-3β and p-p38 MAP kinase.
研究领域:
药理学
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癌症研究
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生物化学
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植物科学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Chu Zhang,Wenyan Wu,Lei Zhou,Huan Cheng,Xingqian Ye,Yong He
Black goji berry ( Lycium ruthenicum Murr.) has great commercial and nutritional values. Near-infrared hyperspectral imaging (NIR-HSI) was used to determine total phenolics, total flavonoids and total anthocyanins in dry black goji berries. Convolutional neural networks (CNN) were designed and developed to predict the chemical compositions. These CNN models and deep autoencoder were used as supervised and unsupervised feature extraction methods, respectively. Partial least squares (PLS) and least-squares support vector machine (LS-SVM) as modelling methods, successive projections algorithm and competitive adaptive reweighted sampling (CARS) as wavelength selection methods, and principal component analysis (PCA) and wavelet transform (WT) as feature extraction methods were studied as conventional approaches for comparison. Deep learning approaches as modelling methods and feature extraction methods obtained good and equivalent performances to the conventional methods. The results illustrated that deep learning had great potential as modelling and feature extraction methods for chemical compositions determination in NIR-HSI.
研究领域:
食品科学
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植物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 10
·1247-1257
·
IF 2.073
Jun Sang,Bing Li,Ya-ya Huang,Qun Ma,Kang Liu,Cui-qin Li
This study aimed to extract and separate total anthocyanins from Lycium ruthenicum Murr. by combining deep eutectic solvents (DES) with macroporous resin chromatography and to develop green analytical methods for the determination of anthocyanins. Choline chloride/1,2-propanediol (1 : 2, mol mol−1) containing 10% water (v/v) was screened as the optimal extraction solvent, and extraction at 52 °C for 45 min using a liquid/solid ratio of 20 : 1 gave the maximum extraction yield of total anthocyanins (4.45 ± 0.07 mg g−1). According to the static/dynamic adsorption and desorption tests, LS-32 macroporous resin has the ability to separate anthocyanins from DES with a recovery ratio above 95%. An off-line heart-cutting two-dimensional HPLC-DAD/ESI-MS method was developed for the qualitative and quantitative analysis of L. ruthenicum anthocyanins using environmentally friendly mobile phases. Ethanol and a tartaric acid solution (0.3 mol L−1) were used as the mobile phases of the first-dimensional HPLC-DAD method, and 15 anthocyanin peaks were detected within 17 min. The second-dimensional HPLC-MS method was used to identify the main anthocyanin peaks in the first dimension. Method validation showed that the developed method was accurate, stable and reliable for the analysis of L. ruthenicum anthocyanins. The present study provides an excellent alternative green method for the preparation and determination of anthocyanins from plant material.
研究领域:
分析化学
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植物化学
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食品科学
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产品关联
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2023
·Vol. 46
·期 21
·2300090
·
IF 3.1
Qiangqiang Jia,Zufan Yang,Qian Wang,Haishan Yang,Xiaofeng Tang,Hongyang Zhang,Lingling Cao,Gong Zhang
It has been proved that purine metabolites are implicated in various biological syndromes and disorders. Therefore, the realization of panoramic detection of purine metabolites will be of great significance to the pathogenesis of purine metabolic disorders. In the present study, an ultra-high performance liquid chromatography with tandem mass spectrometry method was developed for the comprehensive quantification of purine metabolites in rat plasma. The 17 purine metabolites were separated and quantified in the short running time of 15 min. The proposed method was strictly validated by applying SeraSub solution as a matrix and proved to be linear (R 2 ≥ 0.9944), accurate (the recoveries of all analytes ranged from 85.3% to 103.0%, with relative standard deviation values ≤ 9.3%), and precise (the intra- and inter-day precisions were less than 10.8% and 12.4%, respectively). The method was then successfully applied to the qualification of the endogenous purine metabolites in acute gouty arthritis rats, as well as colchicine and anthocyanin-intervened rats. Results showed that uric acid, xanthine, hypoxanthine, and xanthine were considered the key factors of acute gouty arthritis. The established method and measurement of purines in rat plasma might help the investigation of the action mechanisms between purine disorders and related diseases.
研究领域:
药理学
·
分析化学
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生物化学
·
医学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）