BIOSENSORS & BIOELECTRONICS
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2023
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IF 12.6
Liyan Bi,Huangruici Zhang,Wenchao Hu,Jiadong Chen,Yixuan Wu,Hao Chen,Bingqian Li,Zhiyang Zhang,Jaebum Choo,Lingxin Chen
Self-assembled functional nanomaterials with electromagnetic hot spots are crucial and highly desirable in surface-enhanced Raman scattering (SERS). Due to its versatile biological scaffold, the M13 phage has been employed to produce novel nano-building blocks and devices. In this study, we propose a novel M13 phage-based SERS nanocarrier , that utilizes the pVIII capsid in M13 to conjugate Au@Ag core-shell nanorod (Au@AgNR) with linker carboxy-PEG-thiol (M13-Au@AgNR) and the pIII capsid to specifically target Escherichia coli ( E. coli ). The M13-Au@AgNR@DTTC (3,3′- diethylthiocarbocyanine iodide) SERS probe was used to detect E. coli in a concentration range of 6 to 6 × 10 5 cfu/mL, achieving a limit of detection (LOD) of 0.5 cfu/mL. The proposed SERS platform was also tested in real samples, showing good recoveries (92%–114.3%) and a relative standard deviation (RSD) of 1.2%–4.7%. Furthermore, the system demonstrated high antibacterial efficiency against E. coli , approximately 90%, as measured by the standard plate-count method. The investigation provides an effective strategy for in vitro bacteria detection and inactivation.
研究领域:
纳米材料
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生物医学工程
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分析化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2022
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IF 9.221
Jinxiu Hou,Yi Xu,Shasha Sun,Xiaoli Zhong,Chih-Tsung Yang,Xin Zhou
Label-free SPR is rarely used for biomarker detection in real samples due to its low sensitivity. Herein, we report a phage-based SPR (P-SPR) strategy for the detection of carcinoembryonic antigen (CEA) in undiluted serums. M13 phage is genetically engineered to display an anti-CEA single-chain variable region fragment on pIII protein for antigen targeting and to display the gold binding peptide (GBP) at the pVIII protein for gold nanoparticles (GNPs) binding to form the GNPs decorated M13 phage probe that can amplify the plasmon resonance signal. The proposed P-SPR method demonstrated ultrasensitive detection of CEA within 10 min. Under ideal conditions, P-SPR showed exceptional sensitivity with a limit of detection (LOD) of 0.83 fM, which is approximately 2000 times that of the conventional SPR and four orders of magnitude more sensitive than enzyme-linked immunosorbent assay. In particular, the proposed strategy can cross-validate the SPR results through naked-eye counting of the plaques generated by the P-SPR probes, enabling a reliable dual detection system. In addition, P-SPR scheme has excellent sensing performance for the detection of real samples at concentrations lower than LOD of ELISA. Therefore, the proposed P-SPR strategy holds potential for early detection of tumour biomarkers in liquid biopsy.
研究领域:
生物传感器
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纳米技术
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癌症诊断
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分析化学
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分子生物学
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产品关联
DOI
高影响因子
PHYTOMEDICINE
·
2022
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IF 6.656
Chuan Li,Liru Tian,Yihai Wang,Huan Luo,Jia Zeng,Peiqiang Su,Shulin Chen,Zhiheng Liao,Weimin Guo,Xiangjiu He,Shuqing Chen,Caixia Xu
Background Morinda officinalis (MO) is a herb used in Traditional Chinese Medicine (TCM) for the treatment of osteoporosis. M13, a MO-based anthraquinone compound is known to suppress osteoclast activity. However, whether M13 promotes MSCs osteogenic differentiation and its potential mechanism remains unknown. Purpose To examine the influence of M13 on MSCs proliferation and osteogenic differentiation and elucidate the underlying mechanism. Methods/Study designs The effect of M13 exposure on MSCs proliferation was assessed via CCK8 assay, clone formation assay, immunofluorescence, RT-qPCR, and Western blot. The M13-mediated osteogenesis in vitro and ex vivo were evaluated via ALP and Alizarin red S staining, osteogenesis-associated gene (Runx2, Col1a1 and Opn) expression, and fetal limb explants culture. Molecular docking was employed for target signal pathway screening. The potential signaling mechanisms of M13-promoted MSCs osteogenic differentiation were analyzed by introducing XAV939 (Wnt/β-catenin signaling inhibitor). Results M13 induced certain obvious positive effects on MSCs proliferation and osteogenic differentiation. Treatment with M13 enhanced MSCs viability and clone numbers. Meanwhile, M13 promoted osteogenic gene expression, enhanced ALP intensity and Alizarin red S staining in MSCs. In terms of mechanism, M13 strongly interacted with the docking site of the WNT signaling complex, thereby activating the Wnt/β-catenin pathway. Furthermore, the M13-mediated osteogenic effect was partially inhibited by XAV939 both in vitro and ex vivo , which confirmed that the Wnt/β-catenin axis is a critical regulator of M13-induced osteogenic differentiation of MSCs. Conclusion Our study elucidated for the first time that M13 significantly promoted osteogenic differentiation of MSCs via stimulation of the Wnt/β-catenin pathway in vitro and ex vivo .Our findings offered new additional evidence to support the MO or M13-based therapy of osteoporosis.
研究领域:
药理学
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细胞生物学
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分子生物学
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传统中药
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骨生物学
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产品关联
DOI
PubMed
高影响因子
ACS Nano
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2025
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IF 16
Xiaotong Chen,Lei Lei,Jiayao Yan,Xingzhou Wang,Lin Li,Qin Liu,Ying Wang,Tianran Chen,Jie Shao,Lixia Yu,Zijian Li,Lijing Zhu,Lifeng Wang,Baorui Liu
Although personalized neoantigen cancer vaccines have emerged as a promising strategy for cancer treatment, challenges remain to develop immune-stimulatory carriers which allow simultaneous transport of adjuvants and vaccines to lymph nodes (LNs). With inherent immunogenicity, genetic plasticity, and efficiency for large-scale production, M13 phages represent an attractive platform for vaccine delivery as natural bionanomaterials. Here, we report the discovery of an anti-CD40 designed ankyrin repeat protein (DARPin) and propose a bifunctional M13 ph age for neoantigen delivery based on this anti-CD40 DARPin protein (M13CD40). M13CD40-based neoantigen vaccines show improved accumulation and prolonged antigen retention in LNs compared with nontargeting phage vaccines due to the abundance of CD40-positive cells in LNs. Besides the intrinsic immunogenicity of phages, M13CD40-based neoantigen vaccines also benefit from additional CD40 stimulation due to multiple copies of anti-CD40 DARPins displayed on M13CD40 phages. Subcutaneous immunization with M13CD40-based neoantigen vaccines results in more robust antigen-specific immune responses and superior antitumor efficacy in poorly immunogenic tumor models compared with nontargeting phage vaccines. Combination therapy with PD-1 blockade further enhances T cell cytotoxicity and improves tumor control. To summarize, our findings highlight M13CD40 as a CD40 nanoagonist as well as an efficient vehicle for LN-targeted delivery of personalized neoantigen vaccines.
研究领域:
肿瘤学
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免疫学
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纳米医学
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产品关联
DOI
PubMed
高影响因子
ADVANCED MATERIALS
·
2025
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IF 26.8
Zhixin Zhu,Lulu Jin,Qiaoxuan Wang,Haifei Shi,Ke Cheng,Zhengwei Mao
There is a desperate need for precise nanomedications to treat ischemic cerebral injury. Yet, the drawbacks of poor delivery efficiency and off-target toxicity in pathologic parenchyma for traditional antioxidants against ischemic stroke result in inadequate brain accumulation. M13 bacteriophages are highly phagocytosed by M1-polarized microglia and can be carried toward the neuroinflammatory sites. Here, a bio-active, inhalable, Ce 0.9 Zr 0.1 O 2 -backpacked-M13 phage (abbreviated as CZM) is developed and demonstrates how M13 bacteriophages are taken up by different phenotypes’ microglia. With the M1 microglia's proliferating and migrating, CZM can be extensively and specifically delivered to the site of the ischemic core and penumbra, where the surviving nerve cells need to be shielded from secondary oxidative stress and inflammatory cascade initiated by reactive oxygen species (ROS). With non-invasive administration, CZM effectively alleviates oxidative damage and apoptosis of neurons by eliminating ROS generated by hyperactive M1-polarized microglia. Here, a secure and effective strategy for the targeted therapy of neuroinflammatory maladies is offered by this research.
研究领域:
神经科学
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纳米医学
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药理学
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产品关联
DOI
PubMed
高影响因子
Polymer Chemistry
·
2022
·Vol. 13
·期 36
·5200-5211
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IF 5.364
Yuhan Wang,Xiaonan Zheng,Weiting Zhong,Zihan Ye,Xinzhi Wang,Ziyue Dong,Zhenkun Zhang
Anisotropic hydrogel fibers consisting of distinct blocks with heterogeneous internal structures and chemical components are expected to have many advanced functionalities. Based on the chiral liquid crystals (CLCs) of rod-like cellulose nanocrystals (CNCs) and M13 bacteriophages (M13), the current work reports a versatile method to prepare hydrogel fibers with block configurations that have chiral internal structures and multiple components. The effects of confinement on the CLC phases and blockwise photopolymerization were simultaneously explored to address the challenges of how to maintain the delicate chiral ordering of the biological nanorods during gelation and how to seamlessly fuse together the hydrogel blocks containing the CLC phases of different nanorods. The hydrogel precursors containing the CLC phases of either CNCs or M13 were confined in cylindrical glass capillaries and sequentially photopolymerized into hydrogels. Chiral hydrogel fibers with di- or tri-block configurations were prepared, in which hydrogel blocks containing the CLC phases of either CNCs or M13 fuse together seamlessly and arrange alternatively. Due to the unique physiochemical properties specific to CNCs and M13, each hydrogel block has different properties, including chiral fingerprints with various pitches, swelling/deswelling behaviors, mechanical strength, stretchability, strain-sensitive birefringent colors, etc. The synergic effects of these properties endow the CNC–M13 chiral hydrogel fibers with block dependent swelling/deswelling, asymmetric strain deformation and stretchability, which can be tuned by surface chemical modifications specific to the biological rods. These block chiral hydrogel fibers might have applications in multi-task strain sensing, actuations, soft chiral optics, etc.
研究领域:
材料科学
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生物物理学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2026
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IF 11.8
Wenyi Dong,Ajuan You,Yulou Qiu,Wanwan Liu,Jia Qiu,Yutao Zhu,Biao Zhang,Xianshu Fu,Zihong Ye,Xiaoping Yu
Cow's milk allergy is a significant global health concern, with bovine casein recognized as its predominant allergen. Rapid, sensitive, and accurate detection of bovine casein in food products is therefore essential. Here, a triple-signal lateral flow immunoassay (LFIA) for casein detection was developed by integrating an M13 phage-displayed nanobody (M13-Nb) with multifunctional MnO 2 nanosheets (MnO 2 NSs) as the signal probe. Anti-casein M13-Nb was selected from a naïve phage-displayed nanobody library through four rounds of biopanning. MnO 2 NSs exhibiting intrinsic colorimetric properties, oxidase-like catalytic activity, and photothermal capability were synthesized and conjugated with M13-Nb, enabling the MnO 2 NSs@M13-Nb-based LFIA to generate colorimetric, amplified colorimetric, and photothermal signals simultaneously. The visual limits of detection (vLOD) for these modes were 125, 15.63, and 31.25 ng/mL, respectively, with corresponding quantitative LODs of 60.97, 6.72, and 23.88 ng/mL. The assay showed high specificity and accuracy, with recovery rates of 87.92%–114.25% in spiked food samples. This triple-signal LFIA provides a rapid and reliable platform for casein detection in foods and highlights the potential of combining M13-Nb and MnO 2 NSs for on-site monitoring of food allergens.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2026
·
IF 11.8
Wanwan Liu,Wenyi Dong,Yulou Qiu,Yutao Zhu,Jia Qiu,Jianping Wang,Weiwei Qin,Xuping Shentu,Zihong Ye,Xiaoping Yu
Bovine lactoferrin (LF) is widely used as a nutritional additive in infant formula and functional foods, but is also a milk allergen. Therefore, rapid and sensitive detection of LF is crucial for ensuring food quality and protecting public health. Here, we report a multimodal lateral flow immunoassay (LFIA) for LF detection that integrates an M13 phage-displayed nanobody (M13-Nb) with a “four-in-one” multifunctional nanozyme, namely an AuPt nanoparticle-loaded CoCu-embedded nitrogen-doped carbon nanocomposite (CoCu-NC@AuPt). The specific M13-Nb against LF was isolated from a naïve nanobody library. The synthesized CoCu-NC@AuPt exhibits magnetic, colorimetric, peroxidase-mimetic, and photothermal properties, enabling magnetic enrichment and triple-mode sensing (colorimetric, catalytic colorimetric, and photothermal modes). The proposed assay achieved detection limits of 98 pg/mL, 8.49 pg/mL, and 37 pg/mL for the three detection modes, respectively. Practical applicability was verified in pasteurized milk, UHT milk, and yogurt samples, with recoveries of 86.2%–112.5% and coefficients of variation below 15%. This work provides a reliable and sensitive platform for on-site LF detection and demonstrates the potential of combining CoCu-NC@AuPt nanozyme with phage-displayed nanobodies to improve point-of-care testing performance.
✓ 已验证
DOI
PubMed
高影响因子
Nanoscale
·
2021
·Vol. 13
·期 42
·17871-17880
·
IF 7.79
Wenjing Qi,Huimin Yu
Surface immobilization of enzymes on magnetic-recoverable carriers is of great interest and importance for the biocatalysis of relatively large molecules. In this work, the nanosized amino-rich filamentous M13 virus, a versatile biological scaffold, was applied as the unique soft backbone for lipase immobilization. Based on the structure and capsid proteins of M13 phages, the magnetic-recoverable mimic-free-lipases (MFLs) composed of the M13 hydrogels and magnetic particles were developed in two designs. In the first design, nanosized wild M13 phages were crosslinked into a phage hydrogel through the N-terminals of pVIII peptides while NH2-Fe3O4 magnetic nanoparticles (MNPs) were attached to the M13 virus through glutaraldehyde, forming the M13-(NH2-Fe3O4) magnetic phage hydrogel. In the second design, special M13 with Fe3O4 affinity pIII-peptide (FAP-M13) was biopanned for strongly binding towards bare Fe3O4 with the “hook”-like pIII-peptide (N-LPLSTQH-C). TEM observation confirmed the direct grasp of FAP-M13 on bare Fe3O4, forming the magnetic (FAP-M13)-Fe3O4 virus hydrogel. Lipases were uniformly anchored on the phage surface of nanoscale by crosslinking with the N-terminals of pVIII peptides, and then lipase@M13-(NH2-Fe3O4) and lipase@(FAP-M13)-Fe3O4 MFLs were constructed. For both MFLs, high activity recovery yield (>95%) and efficient magnetic separation were characterized. Significantly reduced MNP-usage-amount and enhanced lipase-loading-amount both by about 40 folds were obtained, compared with the conventional NH2-Fe3O4 carriers. The quantified Km and Vmax/Km values were almost equal to those of the free lipases, verifying free-enzyme-mimicking features of the MFLs. High pH-tolerance, wide temperature adaptability, enhanced thermal stability and stable magnetic separation capability of both MFLs were also observed. In particular, the (FAP-M13)-Fe3O4 magnetic virus hydrogel simply using bare Fe3O4 MNPs would be more convenient and economical in the scaled-up biocatalysis.
研究领域:
生物技术
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材料科学
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生物化学
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化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2014
·Vol. 4
·期 3
·413-419
·
IF 4.88
Ye Tian,Man Wu,Xiangxiang Liu,Zhi Liu,Quan Zhou,Zhongwei Niu,Yong Huang
Viral nanoparticles have attracted extensive research interests in diverse applications of diagnosis and therapy. In particular, filamentous M13 bacteriophages have shown great potential in biomedical applications. However, its pathways entering into cells still remain unclear, and this greatly hinders its further use as a drug or gene carrier. Here, a ratiometric M13 pH probe is designed by conjugating two fluorescent dyes onto the surface of M13. Since the intensity ratio is not influenced by probe concentration, ion strength, temperature, photobleaching, and optical path length, this ratiometric probe can be used to investigate the intracellular pH map of M13. More importantly, the internalization mechanism of M13 can be elucidated. It is found that this filamentous phage shows great cell-type dependence in interaction with cells and internalization mechanism. The phage tends to be bounded on the cell membrane of only epithelial cells, not endothelial cells. Furthermore, the M13 phage enters into cells through endocytosis with specific mechanism: clathrin-mediated endocytosis and macropinocytosis for HeLa; vesicular transport, clathrin-mediated endocytosis, and macropinocytosis for MCF-7; caveolae-mediated endocytosis for human dermal microvascular endothelial cell (HDMEC). This work provides key notes for cancer diagnosis and therapy based on filamentous bacteriophage, especially for design of pH-sensitive drug delivery systems.
研究领域:
生物技术
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纳米技术
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细胞生物学
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）