FOOD CHEMISTRY
·
2025
·
IF 9.8
Zhang-Na Qin,Jia-Min Chen,Jia-Wei Li,Zhen-Peng Zhen,Qi-Zhao Chen,Zhi-Qiang Zhang,Gui-Hua Wang,Yu-Feng Gao
The slight structural differences between carbohydrate isomers pose considerable difficulties for their identification and quantification. Herein, a rapid method for the absolute quantification of glucose and fructose isomers using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight tandem mass spectrometry (MALDI-TOF/TOF MS/MS). A novel reagent, 4-trimethylamino-6-(4-methoxy-1-naphthyl)-1,3,5-triazine-2-(3-aminophenylboronic acid) (TMNTA), was synthesized and employed as a reactive matrix. This matrix significantly enhances the ionization efficiency of monosaccharides while avoiding matrix interferences. Glucose- 13 C 6 was utilized as an internal standard for the quantitative analysis of both monosaccharides, exhibiting a strong linear correlation (R 2 ≥ 0.990). By integrating the concentration relationships of glucose and fructose derived from two diagnostic ions produced from the TMNTA-derivatized monosaccharides, this approach facilitates the absolute quantification of glucose and fructose. The method was successfully applied to the absolute quantification of these monosaccharides in honey. The proposed approach offers a simple, rapid, and high-throughput analysis of glucose and fructose isomers in complex samples.
研究领域:
分析化学
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食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Lingpeng Zhan,Xi Huang,Jinjuan Xue,Huihui Liu,Caiqiao Xiong,Jiyun Wang,Zongxiu Nie
Mass spectrometry imaging (MSI) is a powerful technique for investigating the biomolecular locations within tissues. However, the isomeric compounds are rarely distinguished due to inability of MSI to differentiate isomers in the probing area. Coupling tandem mass spectrometry with MSI can facilitate differentiating isomeric compounds. Here MALDI-TOF/TOF tandem mass spectrometry imaging approach was applied to probing the spatial distributions of isomeric disaccharides in plant tissues. First, MS/MS imaging analysis of disaccharide-matrix droplet spots demonstrated the feasibility of distinguishing isomeric species in tissues, by measuring the relative intensity of specific fragments. Then, tandem MS imaging of disaccharides in onion bulb tissues indicated that sucrose and other unknown non-sucrose disaccharides exhibit heterogeneous locations throughout the tissues. This method enables us to image disaccharide isomers differentially in biological tissues, and to discover new saccharide species in plant. This work also emphasizes the necessity of considering isobaric compounds when interpreting MSI results.
研究领域:
植物学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Analysis and Testing
·
2021
·Vol. 6
·期 3
·318-326
·
IF 0
Chen Ya-Shun,Feng Yu-Qi
20-Hydroxyecdysone (20E) derived from plants has a wide range of physiological and pharmacological effects on animals and humans, and rapid and sensitive methods for screening of the endogenous 20E in plants are thus required. Herein, a matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF/TOF MS) method is described for rapid and sensitive determination of endogenous 20E in plants. It is based on the use of the (3-(acridin-9-ylamino) phenyl) boronic acid (AYPBA) as the mass tag to assist the MS and tandem MS (MS n ) analysis of 20E on MALDI-TOF/TOF MS. Good linearity was obtained with a determination coefficient ( R 2 ) larger than 0.99 in the range of 0.025–2.5 μΜ. The limit of detection (LOD) was 2.4 fmol. Acceptable precision and accuracy were gained by intra- and inter-day analysis with relative standard deviations less than 19.5% and relative recoveries ranging from 85.7 to 105.2%. In addition, the AYPBA labeled 20E produced abundant characteristic fragment ions under the high energy collision-induced dissociation, which facilitated the identification of 20E by MS 2 analysis on MALDI-TOF/TOF MS. Using the method, we enabled the identification and quantification of endogenous 20E in four herbs including Cyanotis arachoidea , Achyranthes bidentata , Spinacia oleracea and Chenopodium quinoa willd., demonstrating the feasibility of the proposed method for screening of the endogenous 20E in plants.
研究领域:
植物学
·
分析化学
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产品关联
DOI
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Yuanqing Luo,Xiaoyong Zhao,Zhan Gao,Huiwen Wang,Yaqing Liu,Cheng Guo,Yuanjiang Pan
Oligosaccharides play a key role in many biological functions, and the accurate identification of oligosaccharide structures is an important prerequisite for a comprehensive understanding of the biological functions of oligosaccharides. MALDI-TOF/TOF tandem mass spectrometry has been considered as a potential technique for the structural characterization of oligosaccharides. In this work, palladium nanoparticles decorated thiol-functionalized metal organic framework nanocomposite (UiO-66-(SH) 2 @Pd NPs) was fabricated as an efficient matrix to assist laser desorption/ionization mass spectrometry (LDI-MS) for oligosaccharides analysis. The ionization efficiency of oligosaccharides was significantly improved owning to the synergistic effect of MOF and Pd nanoparticles, which is favorable for further oligosaccharide structure identification. By combining LDI-LIFT-TOF/TOF, 24 oligosaccharide isomers including disaccharides, trisaccharides and tetrasaccharides, were effectively distinguished. In addition, the relative quantification curves for isomeric oligosaccharides were established with good linear correlations. The method was successfully applied to the identification and quantification of sucrose and maltose in three batches of Asian ginseng and American ginseng respectively, showing potentiality of MOF materials and metal nanomaterials assisted structural analysis of oligosaccharide isomers.
研究领域:
分析化学
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质谱学
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纳米技术
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Arabian Journal of Chemistry
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2015
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IF 3.725
Vikramjeet Singh,Amita Nand,Sarita,Jiwen Zhang,Jingsong Zhu
Comparative study for non-specific adsorption of cell lysate and serum on recently developed biosensor surfaces is reported. Different surface chemistries including, polyethylene glycol (PEG), α-cyclodextrin (CD), hydrogel dextran and surface initiated polymerization (SIP) based gold surfaces were taken into account for this study. Various techniques including, surface plasmon resonance imaging (SPRi) and matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry (MALDI−TOF/TOF MS) technique to evaluate the surfaces with Fourier transform infrared spectroscopy (FTIR) for the confirmation of surface fabrication were used. A high non-specific adsorption response of cell lysate and serum was observed on these so-called non-fouling surfaces. The obtained results from this comparative study provided some hope to explore SIP and dextran surface as a universal platform for biosensor applications. SIP produced best result and showed high sensitivity and minimum non-specific adsorption. We believe that this comparative study will surely help the researchers to further upgrade these surfaces and especially SIP to make it more universal for biosensor microarray applications including biomarker discovery in high throughput format.
研究领域:
生物技术
·
医学诊断
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
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