RAPID COMMUNICATIONS IN MASS SPECTROMETRY
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2022
·Vol. 36
·期 11
·e9293
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IF 2.586
Tianze Wang,Yanhong Hao,Suming Chen
Rationale Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has exhibited great advantages in rapid analysis of metabolites. However, the influence of lipid fragments generated by in-source fragmentation (ISD) and/or post-source fragmentation (PSD) on the accurate qualification and quantification of metabolites has not been fully demonstrated. Methods Phospholipid standards and serum extract were analyzed by MALDI MS with both TiO 2 nanoparticle (TiO 2 NP) and 2,5-DHB matrices to illustrate the structures of lipid fragments and their influence on the qualitative and quantitative analysis of metabolites in biological samples. Monophasic and biphasic extraction methods were also compared for their efficiency in removing potential interferents. Results The fragment ions derived from the phosphocholine head group of phosphatidylcholines (PC) interfere with peaks of low molecular weight (LMW) metabolites at both the MS and MS 2 levels. The biphasic extraction system with methanol/chloroform very efficiently removed the interference from PC fragments, and the metabolites choline and carnitine in serum were directly and accurately quantified by MALDI MS by using this biphasic extraction. Conclusions The phospholipids could produce fragment ions through ISD and PSD in MALDI MS with both nanoparticle and organic matrices. The fragments exerted influence on the qualification and qualification of metabolites in serum. By choosing the proper extraction method, the interference from lipid fragments could be efficiently alleviated.
研究领域:
分析化学
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生物化学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
Materials Express
·
2023
·Vol. 13
·期 11
·1922-1928
·
IF 0.7
Yang Jing,Deng Qiuhui,Luo Chunhua,Yang Aofeng,Luo Xiaohu,Guo Meng,Fu Lichun,Zhen Deshuai,Zhou Huang
Holmium (Ho) modified TiO2 (Ho–TiO2) nanocomposites have been prepared by a sol–gel process, and were used as an effective adsorbent and matrix for the enrichment and analysis of bisphenol S and Indigo by matrix-assisted laser desorption/ionization
time-of-flight mass spectrometry (MALDI-TOF MS). The low detection limits of bisphenol S (BPS) and Indigo (ID) are 0.1 pg·mL−1 and 5 pg·mL−1, respectively. Extremely BPS detection could be achieved in tea samples with good recovery (81.5–93.0%).
研究领域:
分析化学
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环境科学
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DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2018
·Vol. 42
·期 5
·1037-1044
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IF 2.415
Wenjuan Zhang,Liyan Jiang,Li Fu,Qiong Jia
Efficient separation and enrichment of low-abundance glycopeptides from complex biological samples is the key to the discovery of disease biomarkers. In this work, a new material was prepared by coating copper tetra(N-carbonylacrylic) aminephthalocyanine and iminodiacetic acid onto poly(glycidyl methacrylate-pentaerythritol triacrylate) monolith. The monolith was applied to polymer monolithic microextraction for specific capture of glycopeptides coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The developed monolith exhibited satisfactory efficiency for glycopeptide enrichment with high selectivity and detection sensitivity. When the tryptic digest of immunoglobulin G was used as the sample, total 24 glycopeptides were identified and the detection limit was determined as 5 fmol. When the approach was applied to the analysis of glycopeptides in the mixture of bovine serum albumin and immunoglobulin G (100:1, m / m ) digests, 16 glycopeptides could still be observed. Moreover, the monolith was successfully applied to the selective enrichment of glycopeptides from human serum digests, exhibiting great practicability in identifying low-abundance glycopeptides in complex biological samples.
研究领域:
分析化学
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蛋白质组学
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生物医学
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产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
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2022
·Vol. 16
·期 1
·132-142
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IF 3.498
Pu Keyuan,Qiu Jiamin,Li Jiaying,Huang Wei,Lai Xiaopin,Liu Cheng,Lin Yan,Ng Kwan-Ming
The problem of adulteration and mislabeling in meat products has raised the public concerns globally. An easy-operation, fast, and robust method that is applicable to routine inspections is urgently needed. This study showed that the MALDI-TOF MS protein profiling of four meat species (beef, chicken, duck, and pork) combining with partial least squares discriminant analysis (PLS-DA) discovered 57 feature peaks for their unambiguous differentiation. Among them, 36 were identified in Uniprot. Based on the linear relation between the intensities of feature peaks, the partial least squares regression was successfully applied to build the prediction models for determining the adulteration ratios of beef meat mixtures containing one of the other three species. Blind tests were applied to evaluate the method and the average prediction accuracy at 94.7% was achieved. Taking duck meat as the adulterant, the detection sensitivity of the method could be down to 5%. Moreover, the method has also been successfully applied to analyze market samples and the results were in agreement with the PCR method, showing the potential of its practical application for qualitative and quantitative analysis of adulterated beef products.
研究领域:
食品科学
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生物化学
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分析化学
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DOI
高影响因子
ARCHIVES OF MICROBIOLOGY
·
2024
·Vol. 206
·期 3
·1-17
·
IF 2.3
Wang Ze,Zhang Wenwen,Wang Ziyan,Zhang Zhixuan,Liu Yan,Liu Songyi,Wu Qiaoli,Saiding Emilaguli,Han Jiaojiao,Zhou Jun,Xu Jiajie,Yi Xianghua,Zhang Zhen,Wang Rixin,Su Xiurong
A new strain of Bacillus velezensis NDB was isolated from Xiangshan Harbor and antibacterial test revealed antibacterial activity of this strain against 12 major pathogenic bacteria. The whole genome of the bacterium was sequenced and found to consist of a 4,214,838 bp circular chromosome and a 7410 bp circular plasmid. Furthermore, it was predicted by AntiSMASH and BAGEL4 to have 12 clusters of secondary metabolism genes for the synthesis of the inhibitors, fengycin, bacillomycin, macrolactin H, bacillaene, and difficidin, and there were also five clusters encoding potentially novel antimicrobial substances, as well as three bacteriocin biosynthesis gene clusters of amylocyclicin, ComX1, and LCI. qRT-PCR revealed significant up-regulation of antimicrobial secondary metabolite synthesis genes after 24 h of antagonism with pathogenic bacteria. Furthermore, MALDI-TOF mass spectrometry revealed that it can secrete surfactin non-ribosomal peptide synthase and polyketide synthase to exert antibacterial effects. GC-MS was used to analyze methanol extract of B. velezensis NDB, a total of 68 compounds were identified and these metabolites include 16 amino acids, 17 acids, 3 amines, 11 sugars, 11 alcohols, 1 ester, and 9 other compounds which can inhibit pathogenic bacteria by initiating the antibiotic secretion pathway. A comparative genomic analysis of gene families showed that the specificity of B. velezensis NDB was mainly reflected in environmental adaptability. Overall, this research on B. velezensis NDB provides the basis for elucidating its biocontrol effect and promotes its future application as a probiotic.
研究领域:
微生物学
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分子生物学
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生物技术
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DOI
PubMed
高影响因子
Journal of Applied Spectroscopy
·
2019
·Vol. 86
·期 1
·89-95
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IF 0.675
Wang Haojiang,Wang Xuan,Bian Wei,Sun Tijian,Cai Zongwei,Wei Jiancong
Core-shell magnetic metal organic nanocrystals were synthesized and used as matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Four nitropolycyclic aromatic hydrocarbons (nitro-PAHs) were successfully detected by using negative-ion MALDI-TOF MS without background interference. Furthermore, the magnetic nanocomposites (MNCs) coated with zeolitic imidazolate framework-8 (ZIF-8) showed excellent adsorption and enrichment capacity, and can be isolated with a magnet and directly spotted on the stainless steel plate for MALDI measurement. It was also analyzed nitro-PAHs in PM2.5 samples using the ZIF-8-coated magnetic nanocomposite as adsorbent and matrix.
研究领域:
分析化学
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环境科学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2019
·
IF 3.571
Xi Gao,Yu Lu,Ming Wei,MeiFang Yang,CaiXia Zheng,Chengjian Wang,Ying Zhang,Linjuan Huang,Zhongfu Wang
The functional role of human milk oligosaccharides (HMOs) is closely associated with their type, composition, and structure. However, a detailed analysis of HMOs is difficult because neutral oligosaccharides (NHMOs) are mixed with sialylated oligosaccharides (SHMOs) in milk. Here, NHMOs were separated from SHMOs by DEAE-52 anion chromatography, and lactose was removed by graphite carbon solid-phase extraction. Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard’s reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD. Sixty-four oligosaccharides were detected: 36 NHMOs, of which 28 were fucosylated, and 28 SHMOs, of which 8 with α-2,3-linked monosialic acid, 2 with α-2,3-linked disialic acid, 10 with α-2,6-linked monosialic acid, 2 with α-2,6-linked disialic acid, and 5 with both α-2,3- and α-2,6-linked disialic acid. These findings provide the groundwork for further characterization of the structure and activity of HMOs.
研究领域:
生物化学
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营养学
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分析化学
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产品关联
DOI
PubMed
高影响因子
ACS Omega
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2020
·
IF 2.87
Yumeng Yang,Dan Gao,Rui Qian,Yuyang Jiang
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) using conventional organic matrices for detection of small molecules has some limitations, such as heterogeneous analyte/matrix co-crystals, as well as interference of matrices in the low-molecular-weight range. In this work, a zeolite framework nanomaterial, TS-1, was applied as a MALDI matrix for the analysis of small molecules by MALDI-MS for the first time. To improve the signal intensity and reproducibility, TS-1 was modified with polydopamine (TS-1@PDA). Using TS-1@PDA as a matrix, organic substances in the low-molecular-weight region such as amino acids, nucleosides, peptides, oligosaccharides, and fatty acids can be detected by MALDI-MS in positive ion mode. Compared with traditional organic matrices like 2,5-dihydroxybenzoic acid (2,5-DHB) and α-cyano-4-hydroxycinnamic acid (CHCA), TS-1@PDA has the advantages including the formation of uniform sample spots, small background interference at low molecular weight, and better salt tolerance. Furthermore, this matrix was employed for the analysis of endogenous glucose in urine samples, and the level of glucose was quantified with a linear range of 0–10 mM (R2 > 0.98). The results demonstrated that TS-1@PDA has the potential to be used as an effective MALDI matrix for the analysis of small molecules in biological samples with excellent reproducibility and moderate sensitivity.
研究领域:
分析化学
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生物医学
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质谱技术
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2014
·
IF 4.517
Xiao Li,Jie Tan,Jiekai Yu,Jiandong Feng,Aiwu Pan,Shu Zheng,Jianmin Wu
Small peptides in serum are potential biomarkers for the diagnosis of cancer and other diseases. The identification of peptide biomarkers in human plasma/serum has become an area of high interest in medical research. However, the direct analysis of peptides in serum samples using mass spectrometry is challenging due to the low concentration of peptides and the high abundance of high-molecular-weight proteins in serum, the latter of which causes severe signal suppression. Herein, we reported that porous semiconductor-noble metal hybrid nanostructures can both eliminate the interference from large proteins in serum samples and significantly enhance the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) yields of peptides captured on the nanostructure. Serum peptide fingerprints with high fidelity can be acquired rapidly, and successful discrimination of colorectal cancer patients based on peptide fingerprints is demonstrated.
研究领域:
医学
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分析化学
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生物技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2015
·
IF 2.979
Hua Li,Dan Li
In this study, a novel molecularly imprinted polymer (MIP) monolith for highly selective extraction of enkephalins was synthesized and prepared in a micropipette tip using epitope imprinting technique. The synthesized MIPs were characterized by scanning electron microscope (SEM) and infrared spectroscopy. A molecularly imprinted solid-phase microextraction (MISPME) method was developed for extraction of enkephalins in aqueous solutions. The parameters affecting MISPME were optimized. The results indicated that this MIP monolith exhibited specific recognition capability, high enrichment efficiency and excellent reusability for enkephalins. MALDI-TOF MS analysis demonstrated that this MIP monolith can act as a useful tool for highly selective purification and enrichment of enkephalin, a kind of low abundance protein, from high-abundance proteins in human cerebrospinal fluids (CSF). Employed this MIP monolith as solid-phase microextraction column, quantitative assay of enkephalins in human CSF was developed by HPLC–ultraviolet (UV) detection in this work. The detection limits were 0.05–0.08 nM. This MISPME/HPLC–UV method was used to quantify Met-enkephalin and Leu-enkephalin levels in the CSF of patients with cancer pain.
研究领域:
分析化学
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医学
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY
·
2017
·Vol. 55
·期 15
·2537-2543
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IF 2.952
Jiatao Wu,Chunyan Tan,Xinwen Zhou,Ying Tan,Pengyuan Yang,Yuyang Jiang
Molecular weights of seven poly(phenylene ethynylene)-based water-soluble conjugated polyelectrolytes (CPEs) obtained through Sonogashira coupling are determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). A standard sample preparation protocol is developed to characterize the seven CPEs using 2,5-dihydroxybenzoic acid as the matrix (M) and AgTFA as the cationization reagent (CR). High-quality MALDI mass spectra are obtained at volume mixing ratios (CPE/M/CR) of 5/5/1 for anionic polymers (P1–P4) and 5/50/1 for cationic polymers (P5–P7). Molecular weight, molecular weight distribution, and end-group information are analyzed. The effects of molecular weight of CPEs on optical and quenching properties are also studied. © 2017 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2017 , 55 , 2537–2543
研究领域:
材料科学
·
化学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
·
2023
·
IF 2.6
Dai Yan,Jia Jia,Kang Tong,Jiuhong Ouyang,Yi Pan
Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) is a widely used method for analysing environmental pollutants and proteins. However, this technology has been limited in its ability to analyse small molecules (<700 Da) due to interference from conventional organic matrix background ions, leading to poor signal-to-noise ratios and reproducibility. In an effort to address this limitation, we have developed a new method for synthesising titanium metal organic frameworks (MOFs) as matrices for MALDI-TOF analysis. Specifically, we used a microwave-assisted facile synthesis method that results in smaller MOF particle sizes and requires less reaction time and simpler procedures. Our MOF, also known as NTU-9, was constructed using 2,5-dihydroxybenzoic acid as a ligand and titanium as a metal site. These constituents make NTU-9 highly efficient for photon absorption and target ionisation, allowing for improved MALDI-TOF analysis of diverse molecules, including environmental pollutants and other small molecules.
研究领域:
分析化学
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环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）