ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2022
·
IF 7.129
Dandan Kong,Xinyue Li,Yang Tang,Ming Sui,Jinping Li,Yonggui Ma,Gaofeng Wang,Wei Gu,Xuegang Guo,Meihua Yang
Environmental pollution and medicine safety have aroused increasing public concerns due to human health. Amongst various contaminants, mercury is of special attention owing to their environmental persistence and biogeochemical recycling and ecological risks. Herein, a simple and highly parallel electrochemical biosensor for Hg determination was designed and investigated. The proposed biosensor was prepared and compared between (1) DTT/MB-DNA/Au with configuration occupation approach and (2) MCH/MB-DNA/Au with passivation approach. According to the combined results of scanning electrochemical microscope (SECM) and Randles-Sevcik equation, the DTT modified electrode exhibited high uniformity on DNA distribution and superb stability on electron transfer in Hg 2+ detection. Evidentially, the response value of proposed DTT/MB-DNA/Au was increased from 57.518% to 97.607%, while RSD% between duplicate runs had dropped from 22.658% to 0.223% (n = 3). Moreover, the increased proportion of effective working area was 467.380% compared with general sensors. Besides, DTT concentration, DNA concentration as well as assembly time were optimized, utilizing electrochemical impedance spectroscopy (EIS), Cyclic Voltammetry (CV) and Square Wave Anode Stripping Voltammetry (SWASV). This optimized biosensor exhibited an excellent selectivity toward Hg 2+ over Cu 2+ , As 2+ , Cd 2+ , Pb 2+ , Cr 3+ , Ni 2+ and Zn 2+ etc., and the stability of DTT/MB-DNA/Au were at least two times better even after 3 days under room temperature. Also, a linear relation was observed between the peak current and Hg 2+ concentrations in a range from 0.25 nM to 2.00 μM with a detection limit of 53.00 pM under optimal conditions. Finally, DTT/MB-DNA/Au was applied for plants and medical products analysis. In all, this optimized DTT/MB-DNA/Au with advantages of high repeatability and sensitivity would provide a new insight into the design and application of biosensor for reliable sensing in safeguarding plant protection and medicinal safety.
研究领域:
环境科学
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生物传感技术
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Oncology Letters
·
2017
·Vol. 14
·期 5
·5307-5312
·
IF 1.39
Binggao Wang,Zhibo Xing,Fengmei Wang,Xinyan Yuan,Yanhui Zhang
In order to improve outcomes after breast cancer treatment, it is essential to understand the mechanisms of action of potential therapeutic agents. The effect of fangchinoline (FAN) on migration and apoptosis of human breast cancer MDA-MB-231 cells and its underlying mechanisms were investigated. MDA-MB-231 cells were treated with different concentrations of FAN, growth inhibition rates were measured by MTT assay and morphological changes of apoptotic cells were observed by Hoechst staining. The wound-healing assay was used to determine of the effect of FAN on the migration of MDA-MB-231 cells. ELISA was used to detect the expression of MMP-2 and -9 in MDA-MB-231 cells treated with different concentrations of FAN and western blot analysis was used to quantify expression of NF-κβ and Iκβ proteins in the same cells. Our results showed that FAN significantly inhibited the growth of MDA-MB-231 cells in concentration-dependent manner and it induced MDA-MB‑231 cell apoptosis. With the high FAN concentrations and long exposure times, the levels of MMP-2 and -9 decreased and the expression of NF-κβ decreased, while the expression of Iκβ protein increased. Based on these results, the antitumor effects of FAN on breast cancer cells can be explained at least partially by inducing apoptosis and inhibiting the migration of MDA-MB-231 cells.
研究领域:
肿瘤学
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药理学
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细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CLINICAL LABORATORY ANALYSIS
·
2018
·Vol. 32
·期 6
·e22426
·
IF 1.303
Zhi-yuan Lin,Yi-zhen Fang,Hong-wei Jin,Hua-yue Lin,Zhang Dai,Qing Luo,Hong-wei Li,Yan-ling Lin,Shui-zhen Huang,Lei Gao,Fei-hai Xu,Zhong-ying Zhang
Background To verify and evaluate the performance characteristics of a creatine kinase phosphokinase isoenzymes MB (CK-MB) assay kit, which produced by Xiamen Innodx Biotech Co. Ltd. Methods Evaluation was carried out according to “Guidelines for principle of analysis performance evaluation of in vitro diagnostic reagent.” The performance parameters included detection limit, linearity range, reportable range, recovery test, precision verification, interference test, cross-reactivity, matrix effect, and method comparison. Results The detection limit was 0.1 ng/mL. The assay had clinical linearity over range of 0.1 ng/mL-500 ng/mL. Reportable range was from 0.1 ng/mL to 1000 ng/mL. The average percent of recovery was 99.66%. The coefficient of variation (CV) for within-run and between-run of low CK-MB sample was 5.55% and 6.16%, respectively. As for high-level sample, it was 7.88% and 7.80%. In medical decision level, the relative deviation (Bias) of all interference tests was lower than 15%. When the sample had mild-hemolysis; hemoglobin ≤15 g/L; triglyceride ≤17 mmol/L; bilirubin ≤427.5 μmol/L; rheumatoid factor ≤206U/mL, there was no significant interference to be found. Moreover, assay kit had no cross-reaction with CK-MM and CK-BB. At last, total diagnostic accuracy of kit was 93.24%, when compared with refer kit. Conclusion Overall the results of the verification study indicated the performance of kit is met the requirements of the clinical test.
研究领域:
医学检验
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2024
·
IF 2.7
Zhiren Zhou,Yuting Tian,Lina Zou,Yanan Liu,Xueqing Zhang,Xiaojing Huang,Huanyu Ren,Zheng Li,Huiru Niu,Hao Liao,Xiaojing Zhang,Hongzhi Pan,Shengzhong Rong,Hongkun Ma
Nuclear matrix protein 22 (NMP22) is one of the most important tumor markers of bladder cancer and is significantly elevated in the urine of bladder cancer patients. Therefore, in this work, a highly sensitive ratiometric electrochemical immunosensor was constructed to detect NMP22 based on ZIF-8@MWCNTs@Chit@Fc@AuNPs composites. ZIF-8 had a large surface area and good adsorption ability. Multi-Walled Carbon Nanotubes (MWCNTs) can optimize the electrical conductivity of ZIF-8, so that the electrode surface of ferrocene (Fc) obtains a stable and strong electrochemical signal. In addition, AuPt-MB provided another strong detection signal methylene blue (MB) while immobilizing the secondary antibody (Ab2) through Au–N and Pt–N bonds. A ratiometric electrochemical sensor was formed based on ZIF-8@MWCNTs@Chit@Fc@AuNPs and AuPt-MB, which showed a great linear connection between IMB/IFc and the logarithmic concentration of NMP22 with a detection limit of 3.33 fg mL−1 (S/N = 3) under optimized specifications in the concentration interval of 0.01 pg mL−1 to 1000 ng mL−1. In addition, the ratiometric immunosensor showed good selectivity and stability.
研究领域:
生物医学工程
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肿瘤学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2021
·Vol. 22
·期 21
·11466
·
IF 5.924
Miaomiao Qi,Xiang Liu,Ying Zhou,Haoyu Wang,Ying Zhao,Jing Ren,Jin Xiang
G protein-coupled estrogen receptor 1 (GPER1) is a potential therapeutic target for treating triple-negative breast cancers (TNBC). However, modulators for GPER1 that can be used to treat TNBC have not appeared. Berberine (BBR) is a bioactive isoquinoline alkaloid with high oral safety. In recent years, BBR has shown an inhibitory effect on TNBC tumors such as MDA-MB-231, but the molecular target remains unclear, which hinders related clinical research. Our work proved that BBR is a modulator of GPER1 that can inhibit cell viability, migration, and autophagy of MDA-MB-231 cells. The inhibitory effect of BBR on MDA-MB-231 cells has a dependence on estrogen levels. Although BBR promoted the proteasome, which is a major factor in the degradation of GPER1, it could still induce the protein level of GPER1. Correspondingly, the transcription of cellular communication network factor 2 (CCN2) was promoted. BBR could bind to GPER1 directly and change the secondary structure of GPER1, as in the case of 17β-estradiol (E2). In addition, BBR induced not only a high degree of co-localization of GPER1 and microtubule-associated protein 1 light chain 3 (MAP1LC3), but also the accumulation of sequestosome 1 (SQSTM1/p62) by the inhibition of the nuclear translocation of the nuclear factor-kappa B (NF-κB) subunit (RELA/p65), which indicates NF-κB inhibition and anti-cancer effects. This result proved that the promotional effect of BBR on the GPER1/NF-κB pathway was closely related to its inhibitory effect on autophagy, which may serve as a new mechanism by which to explain the inhibitory effect of BBR on MDA-MB-231 cells and expand our understanding of the function of both BBR and GPER1.Keywords:berberine;binding;co-localization;GPER1;MAP1LC3;NF-κB
研究领域:
肿瘤学
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药理学
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细胞生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Junhui Du,Haibin Liu,Hongshuo Chen,Chuanjin Cui,Wensi Zhang,Xuechao Zhang,Zhang Bin
A competitive electrochemical aptamer biosensor for the selective detection of Pb 2+ was developed in this study. The PAMAM@Au hybrid materials and the bioprobe MB-GNPs-ssDNA were synthesized with chemical methods . Modified electrode with carboxylated graphene oxide was prepared via electrodeposition . PAMAM@Au was immobilized on the modified electrode using the EDC/NHS crosslinker, and aptamer (Apt) was bound to PAMAM@Au hybrid materials using the glutaraldehyde crosslinker. The assembly of MB-GNPs-ssDNA/Apt/PAMAM@Au/GO/SPCE biosensors was achieved through immobilizing bioprobe onto electrodes, based on the theory of aptamer-complementary chain reactions. The fabricated biosensor was characterized through electrochemical experiments, SEM, AFM , TEM, XPS and FTIR . The biosensor's performance was evaluated in a series of Pb 2+ aqueous solutions by differential pulse voltammetry (DPV), and the results demonstrated this biosensor had excellent repeatability, stability, and specificity. The detection range spanned from 0.5 pM/L to 500 nM/L, with a detection limit of 5 fM/L. Consequently, the proposed biosensor can serve as a reliable analytical tool for the sensitive detection of lead ions .
研究领域:
生物化学
·
分析化学
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2019
·
IF 3.973
Lingli Zhang,Chengguang Wu,Shansong Mu,Wei Xue,Dong Ma
To enhance the chemotherapy effect to MDA-MB-231, a glutathione (GSH)-sensitive amphiphilic hyperbranched poly (amide-amine) (mPEG-PLGA-HPAA) was synthesized, and the anti-cancer drug, paclitaxel (PTX) was then encapsulated into the mPEG-PLGA-HPAA micelles. The mPEG-PLGA-HPAA containing a large number of disulfide bonds could degrade and then consume the GSH intracellularly, which was expected to enhances the sensitivity of MDA-MB-231 cells to PTX. It was found that the mPEG-PLGA-HPAA/PTX nanoparticles could respond to the GSH and showed a GSH-controlled PTX release. Simultaneously, the mPEG-PLGA-HPAA/PTX nanoparticles significantly enhanced the efficacy of PTX by inhibiting MDA-MB-231 cells proliferation and inducing cells apoptosis, performing the self-sensitization effect of chemotherapy. Moreover, the drug carrier of mPEG-PLGA-HPAA exhibited excellent biocompatibility in vitro and in vivo . These results indicated that the self-sensitized drug carrier is a promising route to maximize the therapeutic effect and minimize the side effects of chemotherapy drugs used currently in clinical.
研究领域:
药学
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肿瘤学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2020
·
IF 4.939
Hongmei Zhao,Tingting Zhang,Dongyu Shan,Ying Zhu,Ge Gao,Ye Liu,Jing Liu,Meiying Liu,Wansheng You
An intervening barrier for photocatalytic water decomposition and pollutant degradation is the frustratingly quick recombination of e − - h + pairs. Delicate design of heterojunction photocatalysts by coupling the semiconductors at nanoscale with well-matched geometrical and electronic alignments is an effective strategy to ameliorate the charge separation. Here a facile and environment-friendly l -cysteine-assisted hydrothermal process under weakly alkaline conditions is demonstrated for the first time to fabricate ZnIn 2 S 4 /In(OH) 3 hollow microspheres with intimate contact, which are verified by XRD, SEM, (HR)TEM, XPS , N 2 adsorption-desorption, UV–Vis DRS and photoluminescence spectra. ZnIn 2 S 4 /In(OH) 3 heterostructure (L-cys/Zn 2+ = 4, molar ratio) with a band-gap of 2.50 eV, demonstrates the best photocatalytic performance for water reduction and MB degradation under visible light, outperforming its counterparts (In(OH) 3 and ZnIn 2 S 4 ). The excellent activity of ZnIn 2 S 4 /In(OH) 3 heterostructure arises from the intercrossed band-edge positions as well as the unique hollow structure with large surface area and wide pore-size distribution, which are beneficial for the efficient charge migration from bulk to surface as well as at the interface between ZnIn 2 S 4 and In(OH) 3 . This work provides an efficient and eco-friendly strategy for one-pot synthesis of heterostructured composites with intimate contact for photocatalytic application.
研究领域:
材料科学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Keliang Wu,Pengcheng Wu,Junfang Zhu,Chang Liu,Xuejun Dong,Jianning Wu,Guihua Meng,Kangbo Xu,Juan Hou,Zhiyong Liu,Xuhong Guo
During the photocatalytic production of hydrogen gas, close interface with a catalyst is very important. We designed a noble-metal-free photocatalytic system that is spatially separated to produce hydrogen by high frequency visible light. We used a new type of a catalyst consisting of hollow core-shell CdS@TiO 2 /Ni 2 P structures prepared using sacrificial template method. Photocatalytic decomposition of water was tested using this catalyst. Hydrogen production yield using 420 nm light was 14.80 mmol/g after 2.5 h and using Xe lamp with AM1.5G filter was 34.78 mmol/g after 2.5 h. Hollow core shell CdS@TiO 2 /Ni 2 P particles were more stable than hollow core shell CdS@TiO 2 /Pt structures because TiO 2 protected CdS from photo-corrosion. Combination of CdS and Ni 2 P increased absorption range of TiO 2 at 720 nm. Holes and electrons produced during the photo-reaction can migrate to the CdS on the inside and to the Ni 2 P on the outside of the TiO 2 protective coating. Such design exposes more surface, thus, increases hydrogen production.
研究领域:
材料科学
·
光催化
·
能源转换
·
环境科学
✓ 已验证
DOI
高影响因子
Heliyon
·
2024
·
IF 3.4
Wen-Juan Tang,Di Xu,Ming-Xing Liang,Guan-Qun Wo,Wen-Quan Chen,Jin-Hai Tang,Wei Zhang
Triple-negative breast cancer (TNBC) is more prone to recurrence and metastasis relative to other subtypes of breast cancer, leading to an extremely poor prognosis. The increasing potential chemoresistance of TNBC patients is mainly due to that tumor cells escape from apoptosis. In recent years, statins have demonstrated extensive anti-tumor effects. It is worth noting that statins have more effective anti-tumor effects on TNBC cells and drug-resistant breast cancer cells. Therefore, this study examines the superior cytotoxic effects of statins on TNBC cell lines and further explores their potential therapeutic mechanisms. We detected different cell phenotypes and found that statins significantly reduced the cell viability of TNBC cells. Specifically, pitavastatin showed an obvious induction in cell death, cell cycle arrest and oxidative stress in TNBC MDA-MB-231 cells. The reversal effect of iron chelator desferrioxamine (DFO) on the morphological and molecular biological changes induced by pitavastatin has revealed a new mode of cell death induced by pitavastatin: ferroptosis. This ferroptotic effect was strengthened by the decreased expression of glutathione peroxidase 4 (GPx4) as well as newly discovered ferroptosis suppressor protein 1 (FSP1). The data showed that ferroptotic death of MDA-MB-231 cells is autophagy-dependent and mediated by the mevalonate pathway. Finally, we found that therapeutic oral doses of statins can inhibit the growth of transplanted tumors, which establishes statins as a potential treatment for TNBC patients. In conclusion, we found pitavastatin could induce autophagy-dependent ferroptosis in TNBC cells via the mevalonate pathway which may become a potential adjuvant treatment option for TNBC patients.
研究领域:
肿瘤学
·
药理学
·
细胞生物学
·
癌症研究
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Yuting Zhou,Xiang Chen,Xianlu Lei,Meiqi Huang,Miaomiao Ma,Jian Zeng,Fuyan Zhang,Shuang Li,Qi Sun,Tao Le
Zearalenone (ZEN), a prevalent mycotoxin found in cereal crops, poses a significant threat to food safety and human health. To address this issue, there is an urgent need for rapid, sensitive, and cost-effective detection methods. In this study, we developed a novel bimodal aptasensor based on NH 2 -UiO-66@MB composites for ZEN detection. Methylene blue (MB) was encapsulated within NH 2 -UiO-66 nanoparticles to enhance fluorescence intensity, and the ZEN aptamer was attached to NH 2 -UiO-66 via Zr–O–P bonds and Coulombic forces to create a gating effect. In the presence of ZEN, the aptamer bounded to ZEN, triggering a conformational change that released MB, resulting in decreased fluorescence intensity and absorbance. Under optimal conditions, the bimodal aptasensor exhibited wide linear ranges (2.5–300 ng/mL for colorimetry and 0.125–200 ng/mL for fluorescence) and low limits of detection (1.16 ng/mL for colorimetry and 0.03 ng/mL for fluorescence). Furthermore, the aptasensor demonstrated high specificity and reproducibility, with recovery rates ranging from 97.43 % to 103.71 % in spiked cereal products. These findings highlight the potential of the developed bimodal aptasensor as a novel tool for the rapid, accurate, and cost-effective detection of ZEN in cereal products, contributing to improved food safety monitoring.
研究领域:
食品安全
·
分析化学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
·
2018
·Vol. 106
·期 7
·2544-2552
·
IF 3.373
Chenhui Ji,Zhongzhong Lu,Yonghui Xu,Beibei Shen,Shiyong Yu,Dan Shi
Photodynamic therapy (PDT) holds promise in biochemical study and tumor treatment. A novel multifunctional nanosystem CaO 2 /MnO 2 @polydopamine (PDA)-methylene blue (MB) nanosheet (CMP-MB) was designed. CaO 2 nanoparticles were encapsulated by MnO 2 nanosheet, and then PDA was coated on the surface of CaO 2 /MnO 2 nanosheets, which could adsorb photosensitizer MB through hydrophobic interaction or π-π stacking. In this nanosystem, CaO 2 /MnO 2 had the ability of self-production of oxygen, which solved the problem of tumor hypoxia largely. Moreover, it is worth mentioning that the fluorescence of MB was suppressed by MnO 2 , while its emission was triggered in the simulated tumor microenvironment. Therefore, CMP-MB nanosheet could be used to switch-control cell imaging potentially. 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide testing and Live/Dead assay confirmed CMP-MB nanosheet had fewer side effects without illumination while it destroyed Hela cell with the illumination of light. Vitro cell experiment demonstrated CMP-MB nanosheet could achieve tumor microenvironment responsive imaging and inhibit tumor cell growth under illumination effectively. Therefore, the system has great potential for PDT application and switch-control tumor cell imaging. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2544–2552, 2018.
研究领域:
生物医学工程
·
肿瘤学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）