Journal of Innovative Optical Health Sciences
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2022
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IF 2.396
Xiao Ma,Honglian Xiong,Jinhao Guo,Zhiming Liu,Yaru Han,Mingdi Liu,Yanxian Guo,Mingyi Wang,Huiqing Zhong,Zhouyi Guo
Because the breast cancer is an important factor that threatens women’s lives and health, early diagnosis is helpful for disease screening and a good prognosis. Exosomes are nanovesicles, secreted from cells and other body fluids, which can reflect the genetic and phenotypic status of parental cells. Compared with other methods for early diagnosis of cancer (such as circulating tumor cells (CTCs) and circulating tumor DNA), exosomes have a richer number and stronger biological stability, and have great potential in early diagnosis. Thus, it has been proposed as promising biomarkers for diagnosis of early-stage cancer. However, distinguishing different exosomes remain is a major biomedical challenge. In this paper, we used predictive Convolutional Neural model to detect and analyze exosomes of normal and cancer cells with surface-enhanced Raman scattering (SERS). As a result, it can be seen from the SERS spectra that the exosomes of MCF-7, MDA-MB-231 and MCF-10A cells have similar peaks (939, 1145 and 1380 cm − 1 ). Based on this dataset, the predictive model can achieve 95% accuracy. Compared with principal component analysis (PCA), the trained CNN can classify exosomes from different breast cancer cells with a superior performance. The results indicate that using the sensitivity of Raman detection and exosomes stable presence in the incubation period of cancer cells, SERS detection combined with CNN screening may be used for the early diagnosis of breast cancer in the future.
研究领域:
癌症生物学
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生物医学工程
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医学诊断
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机器学习
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
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2023
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IF 7.4
Qunfei Tai,Hailong Yu,Mingxia Gao,Xiangmin Zhang
From metabolic waste to biological mediators, exosomes have emerged as the key player in a variety of pathological processes, particularly in oncogenesis. The exosome-mediated communication network involves nearly every step of cancer progression, promoting the proliferation and immune escape of cancer cells. Therefore, the removal of cancer-derived exosomes has profound clinical significance. Current methods for exosome separation and enrichment are either for large-scale samples or require complex pretreatment processes, lacking effective methods for trace-volume exosome capture in situ. Herein, we have developed an in situ exosome capturing and counting device based on the antibody-functionalized capillary. Specific antibodies targeting exosome biomarkers were immobilized to the inner wall of the capillary via biotin-streptavidin interaction for direct cancer exosome capturing. Subsequent exosome staining enabled imaging and enumeration. Acceptable linearity and reproducibility were achieved with our device, with the capturing and detective range between 3.3 × 104 and 3.3 × 108 particles, surpassing the nanoparticle tracking analysis by 2 orders of magnitude while requiring merely 30 μL sample. We demonstrated that MCF-7-derived exosomes induced epithelial–mesenchymal transition of epithelial cells MCF-10A, and our method was able to completely or partially reverse the transition by complete depletion or specific depletion of cancer exosomes without any preprocessing. Moreover, both whole exosomes and cancer-specific exosomes alone from mimic blood samples were successfully captured and counted, without obvious non-specific adsorption. In all, our approach realized the in situ depletion and number-counting of cancer-derived exosomes directly from the complex humoral environment, having the potential to provide a comprehensive tumor therapeutic and prognosis evaluation tool by targeted hemodialysis and counting of tumor-derived exosomes.
研究领域:
肿瘤学
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生物技术
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纳米技术
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细胞生物学
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癌症生物学
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产品关联
DOI
PubMed
高影响因子
ANALYST
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2019
·Vol. 144
·期 15
·4575-4581
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IF 4.019
Bo Yin,Sha Sha Li,Shao Hua Lu,Jia Ying Mi,Hong Lin Zhai
Although a large number of fluorescent probes have been developed, the simultaneous quantitative analysis of intracellular thiols is still difficult due to the spectral overlap and the complexity of the intracellular environment. In this study, a multi-signal fluorescent probe was employed for the simultaneous quantification of intracellular glutathione (GSH), cysteine (Cys) and homocysteine (Hcy). As the feature variables of the target components, the Tchebichef image moments (TMs) calculated from the grayscale images of the 3D fluorescence spectra were used to establish the quantitative linear models by stepwise regression. The intra-day and inter-day precisions of the proposed method were less than 5.6% and 8.7%, respectively. The recoveries ranged from 97.0% to 105.9%. In addition, the proposed approach was applied to the simultaneous quantitative determination of Cys, GSH and Hcy in the MCF-10A cell (a type of normal cell) and MDA-MB-231 cancer cell. The obtained results indicated that the concentrations of the three thiols in the cancer cell were higher than those in the normal cell. This study not only provides an effective approach for the quantification of multi-target bio-molecules in complicated intracellular environments, but also further extends the applications of multi-signal fluorescent probes, which will promote the design of new multi-signal fluorescent probes.
研究领域:
分析化学
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生物化学
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癌症研究
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DOI
PubMed
高影响因子
MOLECULES
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2023
·Vol. 28
·期 9
·3935
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IF 4.6
Peng Lu,Xiangjian Cao,Jinghui Zheng,Ying Sun,Ziyu Tang,Meiping Zhao
As a major apurinic/apyrimidinic endonuclease and a redox signaling protein in human cells, APE1 plays a crucial role in cellular function and survival. The relationship between alterations of APE1 expression and subcellular localization and the initiation, development and treatment of various cancers has received extensive attention. However, comparing the in-vivo activity of APE1 in normal and cancerous breast live cells remains challenging due to the low efficiency of commonly used liposome transfection methods in delivering DNA substrate probes into human normal breast epithelial cells (MCF-10A). In this work, we develop a DNA/RNA hybrid-based small magnetic fluorescent nanoprobe (25 ± 3 nm) that can be taken up by various live cells under magnetic transfection. The D0/R-nanoprobe demonstrates an outstanding specificity toward APE1 and strong resistance to the cellular background interference. Using this nanoprobe, we are not only able to visualize the intracellular activity of APE1 in breast ductal carcinoma (MCF-7) live cells, but also demonstrate the APE1 activity in MCF-10A live cells for the first time. The method is then extended to observe the changes in APE1 levels in highly metabolically active neuroendocrine cells under normal conditions and severe attacks by reactive oxygen species in real-time. The fluorescent nanoprobe provides a useful tool for studying the dynamic changes of intracellular APE1 in normal or cancerous live cells. It also displays the potential for visible and controllable release of miRNA drugs within live cells for therapeutic purposes.Keywords:apurinic/apyrimidinic endonuclease 1 (APE1);fluorescent nanoprobe;live cell fluorescence imaging;human normal breast epithelial cells (MCF-10A);human breast adenocarcinoma cells (MCF-7)
研究领域:
癌症生物学
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细胞生物学
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纳米技术
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分子生物学
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DOI
PubMed
高影响因子
TALANTA
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2021
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IF 6.057
Chunrong Li,Yuzhi Xu,Si-Yang Liu,Yanfei Zhang,Wen Yin,Zong Dai,Xiaoyong Zou
Ascorbic acid (AA) and glutathione (GSH), the most abundant intracellular reductive substances, have been widely used as biomarkers for cancer cells identification. The current methods relying on imaging of AA or GSH alone to identify cancer cells may cause systematic errors, since a mutual conversion relationship exists between AA and GSH. In this work, we propose a fluorescent nanosensor for the simultaneous imaging of intracellular reductive substances including AA and GSH. Biocompatible fluorescent silicon nanoparticles (SiNPs) with rich surface amine and carboxyl groups were synthesized. The fluorescence of the SiNP was initially quenched by chelation of Fe 3+ ions, forming SiNP/Fe 3+ complex as the fluorescent nanosensor. Upon the redox reaction with reductive substances, the nanosensor showed sensitively fluorescent recovery. Moreover, benefited from the efficient cellular uptake of the SiNP/Fe 3+ and the overexpressed intracellular reductive substances in cancer cells, the fluorescent nanosensor was used to accurately identify the human breast carcinoma (MCF-7) cells from normal mammary epithelial (MCF-10A) cells by imaging of intracellular AA and GSH simultaneously. This strategy would be promising in imaging-guided precision cancer diagnosis.
研究领域:
癌症生物学
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纳米技术
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生物医学工程
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细胞生物学
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荧光成像
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产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
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2021
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IF 5.097
Siying Liu,Hai-Sheng Su,Zhengxia Yang,Yun Zhang
The identification of cancer cells and tumors significantly advances the accurate diagnosis of cancer and improves the patient survivor rates. However, it remains challenging to achieve the rapid and label-free differentiation of cancer cells from their normal counterparts. Herein, we developed a rapid and effective method for the synthesis of Ag nanorods (NRs) with high uniformity and yield. The surface-enhanced Raman scattering (SERS) substrates demonstrated uniformity and reliability, with a low relative standard deviation (RSD) of 10% and an enhancement factor (EF) of 3.32 × 105. The optimized AgNR SERS substrates were then applied in the quantitative analysis of creatinine and furthermore the bioanalysis of cell lysates for cell classification. Specifically, a high sensitivity with a low detection limit (LOD) of 5.3 μM and a limit of quantification (LOQ) of 17.68 μM was achieved for the detection of creatinine. More importantly, label-free SERS analysis showed the distinct spectral features of the cell lysates for normal breast cell strains (MCF-10A) and breast cancer cell strains (MCF-7, MDA-MB-231). Statistical analysis, such as t-test, principal component analysis (PCA), and orthogonal partial least-squares discriminant analysis (OPLS-DA) aided in the clear discrimination between the normal and cancer groups and further in differentiating the two cancer groups. These results demonstrate the great potential of the combination of a high-quality AgNR SERS substrate and statistical analysis for the identification of cancers.
研究领域:
纳米技术
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癌症诊断
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生物医学工程
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分析化学
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生物信息学
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产品关联
DOI
高影响因子
ANALYST
·
2023
·Vol. 148
·期 10
·2352-2361
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IF 4.2
Xiwen Li,Peiran Meng,Mingyang Sun,Yue Chen,Zhiyi Song,Xinyao Wang,Na Li,Yue Sun
Phospholipase C (PLC) has important biological functions in specific cancer types, immune disorders and neurodegeneration. Here, an ultrasensitive electrochemical sensor for PLC was developed via signal amplification based on breathing atom transfer radical polymerization (ATRP). First, phosphatidylethanolamine (PE) was immobilized on the surface of a gold electrode by L-cysteine and cross-linker. Then, PE was specially hydrolyzed by PLC to obtain the phosphate groups and tethered with the ATRP initiator α-bromophenacetic acid (BPAA) by the coordination action of Zr4+. After the breathing ATRP, a large number of electroactive monomers (ferrocenylmethyl methacrylate, FcMMA) were successfully grafted from BPAA. The experimental results indicated that the detection signal of the obtained electrode (sensor) was proportional to the concentration of PLC. The sensor showed a low detection limit of 0.270 U L−1 and a wide linear range of 1–40 U L−1 (R2 = 0.997). Most importantly, the sensor was successfully applied to detect PLC in breast cancer cells (MCF-7, MDA-MB-231) and nontumor cells (MCF-10A). The value obtained by our electrochemical sensor had no obvious difference from that determined by the commercial ELISA kit. These results showed that the fabricated PLC sensor had acceptable potential in clinical applications.
研究领域:
生物化学
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生物技术
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分析化学
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癌症研究
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免疫学
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神经科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2025
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IF 6.7
Chenyu Zhang,Zutao Chen,Guohua Qi,Yu Tian,Xiongjian Zheng,Xingkang Diao,Jiao Kong,Xingkai Ju,Jing Li,Shaojun Dong,Yongdong Jin
Accompanying the occurrence of inflammatory reaction to release cytokines, pyroptosis can activate an immune response for resistance against cancer. Consequently, elevated levels of cytokines released by cancer cells are highly correlated with the effectiveness of cancer treatment. Herein, a noninvasive surface-enhanced Raman spectroscopy (SERS) immunosensor was developed to sensitively and specifically measure the tumor necrosis factor-α (TNF-α), a proinflammatory cytokine, during the cell pyroptosis process. The sandwiched structure of the sensor is functionalized with a TNF-α binding antibody for detecting TNF-α at concentrations as low as 1 pg/mL. Importantly, electrical stimulation (ES) can fleetly trigger cancer cell pyroptosis to induce the overexpression of receptor interacting protein 3 (RIP3), which is a significant protein that regulates the inflammatory response. The overexpression of RIP3 can activate caspase-1 to promote the upregulation of cytokine levels. Notably, the cytokine levels of TNF-α released from cancer cells (MCF-7 cells) were apparently higher than those of normal cells (MCF-10A cells) during pyroptosis detected by the SERS immunosensors. Due to its obvious superiorities of simple fabrication and fast readout without sample pretreatment, the developed SERS platform has a potential application value for diagnosis and treatment of cancer.
研究领域:
生物医学工程
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癌症生物学
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免疫学
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分析化学
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产品关联
DOI
PubMed
高影响因子
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