Sugar Tech
·
2021
·Vol. 24
·期 3
·832-840
·
IF 1.591
Yang Yujiao,Zheng Rui,Zhang Pingling,Wen Jing,Luo Yue,Zhao Zhengang,You Lijun,Ho Chi-Tang
Sugarcane is one of the most important crops in the world and rich in polyphenols. In this study, quercetin, apigenin, luteolin, tricin and p -coumaric acid, the major polyphenols in sugarcane, were used to evaluate the antiproliferative activities toward MCF-7 human breast cancer cells independently and in combination. These five polyphenols all showed inhibitory effects against MCF-7 cells within different dose ranges. The combinations of luteolin and three polyphenols (quercetin, apigenin, p -coumaric acid) were studied. p -Coumaric acid together with luteolin exhibited synergistic effects at all concentrations tested: the CI (Combination Index) values at 50%, 75% and 90% inhibition of MCF-7 cell growth were 0.68 ± 0.06, 0.70 ± 0.08 and 0.83 ± 0.09, respectively. Although the two-way combination of luteolin and apigenin showed moderate antagonistic effect (CI, 1.26 ± 0.24) at EC 50 , the CI value dropped as the inhibition rate increased, showing a synergistic effect at the inhibition rate above 70%. The combination of luteolin and quercetin indicated antagonistic effect at the inhibition rate above 50% (Fa 0.5, CI = 1.10 ± 0.05). p -Coumaric acid with quercetin exhibited antagonistic actions (Fa 0.5, CI = 1.62 ± 0.22) at all concentrations tested. In addition, the EC 50 values of luteolin and quercetin against MCF-7 cells decreased by 1.91 and 2.62-fold after combining with 20 µM tricin. The results provide evidence of synergistic relation among polyphenols present in sugarcane in inhibition of cancer cell growth and of their potential clinical application.
研究领域:
肿瘤学
·
植物化学
·
药理学
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 10
·1-9
·
IF 5.705
Wang Lude,Chen Yang
A colorimetric and fluorescent pH probe was designed by doping carbon dots (C-dots) with Eu(III), Tb(III) and 2,6-pyridinedicarboxylic acid (DPA). The resulting nanoparticles were applied as fluorescent indicators for pH values (best detected at excitation/emission wavelengths of 272/545, 614 nm). The pH induced optical effects are due to pH induced variations in energy transfer. The fluorescence of the probe shows a continuous color variation, and a linear change with pH values in the range from 3.0 to 10.0 can be established by using a Commission Internationale de L’Eclairage (CIE) chromaticity diagram. This new kind of pH nanoprobe is more accurate than previously reported pH indicator probes because the pH value can be calculated by using chromaticity coordinates that only depend on the chromaticity. The pH nanoprobe was applied to visualize pH values in human breast adenocarcinoma cells (MCF-7).
Carbon dots modified with Eu(III) and Tb(III) complexes of 2,6-pyridinedicarboxylic acid (DPA) were prepared. The doped carbon dots were used as a pH-sensitive nanosensor. The fluorescence chromaticity of the nanoparticles changes with the variation of pH value.
研究领域:
生物化学
·
材料科学
·
医学检测
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产品关联
DOI
PubMed
高影响因子
Future Medicinal Chemistry
·
2022
·Vol. 14
·期 9
·
IF 4.767
Jiao Liu,Xiaoxuan Wang,Yuan Cheng,Yinghua Yu,Maofang Zhao,Jie Huang,Peiquan Yu,Qian Fu,Yilin Song,Yi Liu
Background: H2S is the third gas transmitter affecting the growth, reproduction and survival of cancer cells. However, the H2S anticancer and antitumor mechanism still needs to be further studied. Methods: Here, FHS-1 was synthesized utilizing excited-state intramolecular proton transfer to detect H2S in MCF-7 cells, and investigated the effects of varying concentrations NaHS on apoptosis. Results: The study found that FHS-1 detects H2S levels with high selectivity and pH stability and that H2S may regulate apoptosis in MCF-7 cells through the p53/mTOR/STAT3 pathway. Conclusion: Researching the influence of H2S on apoptosis can serve as a theoretical foundation for future research into H2S-related anticancer medicines, and the H2S probe can be used as an effective cancer screening tool.Graphical abstract
研究领域:
癌症生物学
·
细胞信号传导
·
药物化学
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产品关联
DOI
PubMed
高影响因子
SAUDI PHARMACEUTICAL JOURNAL
·
2017
·
IF 2.302
Yubin Ji,Miao Yu,Zheng Qi,Di Cui,Guosong Xin,Bing Wang,Weiling Jia,Lin Chang
As research was conducted on the early apoptosis of human breast cancer cell MCF-7 caused by lycorine hydrochloride and the expression of the related apoptosis proteins. The early-period apoptosis rate of human breast cancer cell MCF-7 was tested with the AnnexinV/PI double staining and flow cytometry. The Western Blotting method was also used to detect the protein expression conditions of Fas, FasL, Caspase-8 and Bid. The results showed that the higher the dose, the higher the rate of apoptosis and that the rate of apoptosis was dependent on the dose; the relative protein activity of Fas, FasL, Caspase-8 and bid gradually rose with the increase of lycorine dosage and the activities revealed certain dose-independence. Results showed that lycorine hydrochloride could induce the apoptosis of human breast cancer cell MCF-7 through the death receptor pathway.
研究领域:
肿瘤学
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-8
·
IF 4.259
Yang Yufang,Shen Dongjun,Long Yijuan,Xie Zhixiong,Zheng Huzhi
Ficin is classified as a sulfhydryl protease isolated from the latex of fig trees. In most cases, a particular enzyme fits a few types of substrate and catalyzes one type of reaction. In this investigation, we found sufficient proofs for the intrinsic peroxidase-like activity of ficin and designed experiments to examine its effectiveness in a variety of scenarios. Ficin can transform peroxidase substrates to colored products in the existence of H 2 O 2 . Our results also indicate that the active sites of peroxidase-like activity of ficin are different from that of protease, which reveals that one enzyme may catalyze more than one kind of substrate to perform different types of reactions. On the basis of these findings, H 2 O 2 releasing from MCF-7 cells was detected successfully. Our findings support a wider application of ficin in biochemistry and open up the possibility of utilizing ficin as enzymatic mimics in biotechnology and environmental monitoring.
研究领域:
生物化学
·
酶学
·
生物技术
·
环境监测
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产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
·
2021
·Vol. 11
·期 10
·378
·
IF 5.519
Jinfeng Quan,Yihan Wang,Jialei Zhang,Kejing Huang,Xuemei Wang,Hui Jiang
Arch-cruciform DNA are self-assembled on AuNPs/VS2scaffold as a highly sensitive and selective electrochemical biosensor for michigan cancer foundation-7 (MCF-7) breast cancer cells. In the construction, arch DNA is formed using two single-strand DNA sequences embedded with the aptamer for MCF-7 cells. In the absence of MCF-7 cells, a cruciform DNA labeled with three terminal biotin is bound to the top of arch DNA, which further combines with streptavidin-labeled horseradish peroxidase (HRP) to catalyze the hydroquinone-H2O2reaction on the electrode surface. The presence of MCF-7 cells can release the cruciform DNA and reduce the amount of immobilized HRP, thus effectively inhibiting enzyme-mediated electrocatalysis. The electrochemical response of the sensor is negatively correlated with the concentration of MCF-7 cells, with a linear range of 10~1 × 105cells/mL, and a limit of detection as low as 5 cells/mL (S/N = 3). Through two-dimensional materials and enzyme-based dual signal amplification, this biosensor may pave new ways for the highly sensitive detection of tumor cells in real samples.Keywords:MCF-7 cells;electrochemistry;2-D materials;signal amplification;DNA assembly
研究领域:
生物传感
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肿瘤检测
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chemistry-An Asian Journal
·
2014
·Vol. 9
·期 8
·2272-2280
·
IF 3.935
Yan-Jie Guo,Fen Yang,Lu Zhang,Jiang Pi,Ji-Ye Cai,Pei-Hui Yang
Graphical Fighting cancer : Quercetin surface-functionalized germanium nanoparticles (Qu-GeNPs) with enhanced antioxidant and anticancer activity were synthesized by a simple method (see scheme). In vitro drug release of Qu from Qu-GeNPs indicated that Qu could principally be distributed around tumor tissues, and Qu-GeNPs were internalized by MCF-7 cells. A simple method for preparing quercetin surface-functionalized germanium nanoparticles (Qu-GeNPs) with enhanced antioxidant and anticancer activity is reported. Spherical germanium nanoparticles (GeNPs) were capped by quercetin (Qu) with a mean particle size of approximately 33 nm and were characterized by TEM, AFM, UV-visible absorption spectroscopy, FTIR, and XRD measurements. The in vitro drug release of Qu from the Qu-GeNPs indicated that Qu could principally be distributed around tumor tissues rather than in the normal section and Qu-GeNPs were internalized by MCF-7 cells. Their biological activity test results indicated that these Qu-GeNPs possessed stronger hydroxyl-scavenging effects and proliferative inhibition effect on MCF-7 cancer cells than quercetin, thus suggesting that the strategy to use GeNPs as a carrier of Qu could be an efficient way to achieve enhanced antioxidant and anticancer activity. In addition, Qu-GeNPs possessed a high apoptotic induction effect in cancer cells, especially in high dosages, and could arrest MCF-7 cells in the S phase.
研究领域:
纳米医学
·
药物递送
·
肿瘤学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 21
·15549
·
IF 5.6
Weihong Lai,Jiaxin Chen,Xinjiao Gao,Xiaobao Jin,Gong Chen,Lianbao Ye
Chalcone is a common simple fragment of natural products with anticancer activity. In a previous study, the research group discovered a series of chalcone derivatives with stronger anticancer activities. To find better anticancer drugs, novel chalcone derivativesA1–A14,B1–B14have continuously been designed and synthesized. The antiproliferative activity of these compounds against breast cancer cells (MCF-7) was investigated by the Cell Counting Kit-8 (CCK-8) method with 5-fluorouracil (5-Fu) as the control drug. The results showed that compoundA14exhibited excellent antiproliferative ability compared to the control drug 5-Fu. Scratch experiments and cloning experiments further confirmed that compoundA14could inhibit the proliferation and colony formation activity of MCF-7 cells. In addition, molecular docking primarily explains the interaction between compound and protein. These results suggested that compoundA14could be a promising chalcone derivative for further anti-breast cancer research.Keywords:chalcones;synthesis;anti-breast cancer (MCF-7);molecular docking
研究领域:
药理学
·
肿瘤学
·
药物化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2025
·
IF 3.1
Yingxia Guo,Meichen Liu,Yajie Cheng,Jiye Tian,Chunpeng Feng,Qingbin Wang,Xuan Zhao,Lei Yin
Methoxy-polyethylene glycol amine (mPEG-NH₂), an important pharmaceutical excipient, has been extensively utilized in the field of biomedicine. To advance the development of mPEG-NH₂-related drug delivery systems, it is crucial to understand the safety profile and cellular pharmacokinetic behavior of mPEG-NH₂ polymers. In this study, a straightforward analytical assay based on ultra-high performance liquid chromatography and tandem mass spectrometry (UHPLC-MS/MS) for quantifying mPEG 5 -NH₂ in biological matrices was established and validated. Multiple reaction monitoring (MRM) transitions were selected for quantification of mPEG 5 -NH₂ (mass-to-charge ratio, m/z 252.1 → 87.7) and octaethylene glycol (OH-PEG 8 -OH) (mass-to-charge ratio, m/z 371.2 → 89.2). The UHPLC-MS/MS assay demonstrated excellent linearity within the concentration range of 0.01–10 μg/mL, with an R² value of 0.9996. Both intra-day and inter-day accuracies and precisions of the analytical method were within ± 9.19 %. This analytical assay was successfully applied to study the in vitro cellular toxicity and uptake behavior of mPEG 5 -NH₂ in MCF-7 cells. The results indicate that high doses of mPEG 5 -NH₂ may have potential toxicity to MCF-7 cells.
研究领域:
药物分析
·
毒理学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
STEROIDS
·
2017
·
IF 2.282
Xiao-Long He,Yajing Xing,Xiang-Zhong Gu,Jie-Xin Xiao,Ying-Ying Wang,Zhengfang Yi,Wen-Wei Qiu
In this paper, a new and concise synthetic route of lithocholic acid (LCA) using commercially available steroid source deoxycholic acid is reported. A series of amide derivatives of LCA were also synthesized and investigated for their activity against the growth of MCF-7 and MCF-7/ADR cells using the sulforhodamine B assay. For MCF-7, the most potent compound 20 showed a 20-fold higher antitumor activity than LCA. For MCF-7/ADR, the most potent compound 24 showed a 22-fold higher antitumor activity than LCA. The transwell migration assay of 20 was evaluated on MDA-MB-231 cells. The colony formation and apoptosis assays of 20 were performed on MCF-7 and MCF-7/ADR cell lines.
研究领域:
药理学
·
有机合成化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
American Journal of Translational Research
·
2021
·
IF 4.06
Xu Xiao,Xiaolei Hou,Wenda Shi,Chanchan Hu,Yanan Cui,Jiuli Hu,Zongfang Piao,Xiaoqin Zhu,Qingshan Li,Fan Xu
Objective: Oxymatrine has shown strong anti-cancer ability, but its mechanism is not well-studied. Methods: The inhibitory rates of oxymatrine with various concentrations (0, 1, 2, 4, 6, 8, 16, 32 mg/ml) on MCF-7 cells were detected by CCK-8. The effects of oxymatrine on the expression of miRNA-140-5P in MCF-7 cells were detected by real-time fluorescent quantitative PCR (RT-PCR). miRNA-140-5P mimics or NC mimics were transfected into cells using Lipofectamine 2000. Eventually, the cells were divided into control-group, drug-group, miRNA-140-5P mimics group, NC mimics group, and miRNA-140-5P mimics + drug group. Cell viability was detected by CCK-8 assay and apoptosis rate of each group were measured by using Flow cytometry. Western blot was carried out to detect the protein expression of TGFBR1 and FGF9. Results: Oxymatrine at various concentrations had conspicuous inhibitory effect on the proliferation of MCF-7 cells ( P<0.05 ), and the inhibitory effect of oxymatrine on MCF-7 cells showed both dose- and time-dependent manners. The relative expression of miRNA-140-5P in MCF cells was remarkably lower than that in MCF-10A. Oxymatrine could effectively promote the expression of miRNA-140-5P in MCF-7 cells, and the relative expression of miRNA-140-5P increased significantly with the increased dose of oxymatrine ( P<0.05 ). Both transfection of miRNA-140-5P mimics and oxymatrine treatment could reduce the proliferation of MCF-7 cells ( P<0.05 ), and the proliferation of cells in miRNA-140-5P mimics + drug-group was significantly lower than that of other groups ( P<0.05 ). Compared with the control-group, the protein expressions of TGFbR1 and FGF9 in low-dose, medium-dose and high-dose groups were dramatically decreased ( P<0.05 ), in a dose-dependent manner ( P<0.05 ). Conclusion: Oxymatrine inhibits proliferation and promotes cell apoptosis of breast cancer MCF-7 cells. The mechanism may contribute to the regulation of miRNA-140-5p and its target genes.
研究领域:
肿瘤学
·
分子生物学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
Microsystems & Nanoengineering
·
2025
·Vol. 11
·期 1
·1-10
·
IF 9.9
Ma Yanmei,Li Xinhao,Hu Weikang,Ruan Muyang,Yang Ming,Chang Lingqian,Gu Hongri,Hu Chengzhi
Ferroptosis is an iron-dependent form of regulated cell death driven by phospholipid peroxidation and the accumulation of reactive oxygen species (ROS), holding significant importance for therapeutic applications via its induction or inhibition. Accurate detection of intracellular Fe 2+ and ROS is essential, as these molecules play essential roles in initiating and propagating ferroptosis. In this study, we present a novel electrochemical nanoprobe for real-time, highly selective detection of intracellular Fe 2+ . The nanoprobes are prepared by coating gold nanoparticles (AuNPs) and poly(3,4-ethylenedioxythiophene) (PEDOT) onto silicon carbide nanowires (SiC NWs), which are subsequently functionalized with ferrocenyl endoperoxide carboxylic acid (FDCA) and integrated with a liquid metal-filled glass nanopipette. FDCA is specifically synthesized to enable precise electrochemical detection of Fe 2+ with high selectivity (0.1 nM to 1 µM) and exceptional specificity. PEDOT and AuNPs can improve electrical conductivity and provide a versatile interface for further FDCA decoration. We use the nanoprobes to evaluate the intracellular change of Fe 2+ in MCF-7 breast cancer cells during erastin-induced ferroptosis. We observe a significant increase in intracellular Fe 2+ levels in MCF-7 cells undergoing ferroptosis, accompanied by a notable rise in ROS levels. These findings underscore the potential of this nanoprobe to enhance our understanding of the mechanism of ferroptosis in tumor development and as a potential treatment target.
研究领域:
生物化学
·
细胞生物学
·
肿瘤学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）