MICROPOROUS AND MESOPOROUS MATERIALS
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2020
·
IF 4.551
Zongjian Liu,Zhicong Sun,Zhao Zhang,Lifei Xu,Lin Zhang,Qun Cui,Haiyan Wang
UiO-66-Z with uniform mesopores using 2-aminoterephthalic acid and terephthalic acid as mixed ligands was synthesized by hydrothermal method in this paper. Thermodynamic and dynamic properties of UiO-66-Z were discussed. Dynamic separation performance and adsorption selectivity of UiO-66-Z for the equimolar n-heptane/methylcyclohexane (nHEP/MCH) binary mixture were investigated. Results show that UiO-66-Z has complete tetrahedral cages with uniform mesopores of 38 Å. Adsorption rate constant of MCH on UiO-66-Z (14.5 × 10 −3 S −1 ) is twice that of UiO-66 (6.7 × 10 −3 S −1 ), while nHEP does not significantly change at 323K with the pressure of 0.05–0.1 kPa. Effective adsorption diffusion coefficient of nHEP and MCH on the UiO-66-Z is about 25 times higher than that on the UiO-66. Compared with UiO-66, the desorption activation energy (Ed 1 ) of the first desorption peak of nHEP and MCH on the UiO-66-Z is reduced by 53.75% and 76.66%, respectively. The separation selectivity of MCH to nHEP on the UiO-66-Z reaches up to 3.13 at 473 K. Results indicate that the introduction of mesopores in the UiO-66-Z can enhance mass transfer of nHEP and MCH in the pores, especially which is more obvious mass transfer effect for MCH with a larger molecular dynamics diameter under low partial pressure. The retention of most tetrahedral cages in the UiO-66-Z is conducive to improving its inverse shape separation selectivity for MCH/nHEP. Compared with UiO-66, nHEP and MCH have higher desorption efficiency on UiO-66-Z at the same temperature and time.
研究领域:
化学工程
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材料科学
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物理化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF BIOLOGICAL CHEMISTRY
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2025
·
IF 3.9
Qingquan Guo,Yudong Zhang,Jianhua Zhang,Xiaoyu Tian,Yawen Zhou,Yaxin Wang,Mingjie He,Lu Chen,Jiaqi Zeng,Chuanjin Tang,Qiuru Li,Zhenming He,Bingji Ma,Chenyang Jiang,Haishan Zhao
Melanin-Concentrating Hormone (MCH) is a cyclic peptide initially isolated from salmon and later found to be conserved in mammals. It plays a role in regulating melanin changes and rhythmic behaviors such as sleep and feeding, though its relationship with these processes is not fully understood. Our preliminary research revealed significant differences in melanin degradation in zebrafish under varying light conditions, suggesting a link to MCH. This study aims to explore MCH's role in lighting-induced changes in rhythmic behavior patterns and melanin of zebrafish. Using the zebrafish model, we evaluated MCH expression under different lighting conditions and analyzed the effects of arousal-promoting and sleep-inducing agents. We also investigated the impact of exogenous MCH and its inhibitors on melanin degradation, behavioral changes, and differences in MCH expression to uncover potential regulatory relationships between MCH, sleep pressure, and melanin. In-depth research using flow cytometry, AO staining, Lyso-Tracker Red staining, and RT-qPCR analysis of autophagy- and apoptosis-related genes showed that melanin degradation regulation depends on MCH expression levels. Sleep pressure can intervene in MCH's effects, forming a regulatory loop to jointly regulates melanin degradation. The influence of the MCH-sleep pressure loop on melanin degradation is closely tied to autophagic and lysosomal pathways. Our findings reveal a mutually regulatory loop in zebrafish between MCH and sleep pressure, affecting melanin degradation through these pathways.
研究领域:
神经生物学
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细胞生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
TALANTA
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2018
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IF 4.244
Meichuan Liu,Hongyang Ke,Caiqin Sun,Guoqiang Wang,Yu Wang,Guohua Zhao
In the present work, a convenient signal-on electrochemical label-free aptasensor for 17β-estradiol (E2), a typical steroidal hormones endocrine disrupting chemicals , was proposed. 6-mercapto-1-hexanol (MCH) self-assembled monolayer (SAM) modified Au (MCH/Au) electrode was used as the substrate electrode. Graphene is used with bi-functions, not only to adsorb E2 binding aptamer, serving as the recognition element to E2, but also to be assembled onto MCH/Au electrode when sensing E2, to controllably turn on the electron transfer (eT), and further indicate the signal to E2 concentration. With the synergistic effect of DNase I enzyme, highly sensitive detection of E2 was achieved at this aptasensing system, with a linear range from 0.07 to 10 pM and a detection limit of 50 fM. An outstanding selectivity towards E2 was proven for the sensing system by simultaneously detecting 100-fold potential co-existing interferences. The stability and reproducibility were also evaluated to be satisfactory. Spiked real water analysis further indicated its reliability and potential in practical environmental monitoring.
研究领域:
分析化学
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环境科学
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纳米技术
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生物化学
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2018
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IF 5.667
Xing-Pei Liu,Pan-Pan Li,Chang-jie Mao,He-Lin Niu,Ji-Ming Song,Bao-Kang Jin
A novel ultrasensitive photoelectrochemical aptasensing was developed to detect mucin 1 (MUC1) based on TiO 2 /CdS:Eu nanocomposite. The TiO 2 /CdS:Eu nanocomposite modified fluorine-doped tin oxide (FTO) electrode was prepared by successive ionic layer adsorption and reaction technique (SILAR) and then was employed as matrix for immobilization of SH-DNA (DNA1). After 6-mercaptohexanol (MCH) blocked unbound sites of the electrode surface, the MUC1 aptamer(DNA2) was introduced, allowing MUC1 aptamer to hybridize with DNA1. Subsequently, CdS-COOH QDs as signal amplification elements were successively labeled on the terminal of MUC1 aptamer. In this case, the target MUC1 detection was based upon the photocurrent change originated from quantities change of the signal amplification elements CdS. In the presence of MUC1, the MUC1 aptamer was specifically bound with MUC1, and meanwhile MUC1/DNA2/CdS was released from the electrode surface, resulting in significantly decreased photocurrent intensity. The novel ultrasensitive photoelectrochemical aptasensing presented a highly selective, sensitive and reproductive detection of MUC1 from 1 nM to 5 μM with a lower detection limit of 0.3 nM. At the same time, it also provided a good specificity, reproducibility, stability and promising strategy to detect a variety of other protein targets for early clinical diagnosis of different diseases.
研究领域:
生物化学
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分析化学
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生物传感器
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纳米技术
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癌症诊断
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产品关联
DOI
高影响因子
Analytical Methods
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2021
·Vol. 13
·期 38
·4442-4451
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IF 2.896
Huanhuan Li,Tongji Cai,Yi Ren,Jing Huang,Hanbing Jiang,Yucui Hou,Chunhua Tang,Jie Yang,Jia Zhao,Peng Yu
As an essential biochemical indicator in the fields of pregnancy and oncology, human chorionic gonadotropin (HCG) can be evaluated using colloidal gold immunochromatographic paper and quantified using a biochemical analyzer based on the principle of the antibody sandwich method. In view of the inaccuracy of the former and the complication of the latter, this study constructed an accurate, sensitive and simple unlabeled biosensor based on peptide aptamer CGGGPPLRINRHILTR for HCG detection. Molecular Operating Environment (MOE) was used to simulate the aptamer and protein docking, and western blot (WB) was used to verify the binding effect and ratio. The peptide aptamer was characterized and was then reduced with tris-(2-carboxyethyl)-phosphine hydrochloride (TCEP). After electrochemical deposition of chloroauric acid on the screen-printed electrode (SPE), the aptamer was self-assembled on the electrode surface under optimal conditions. The active site of the electrode surface was blocked with 6-mercapto-1-hexanol (MCH) and BSA. The electrochemical impedance spectrum (EIS) was used to quantify HCG in the matrix. Showing a good linear relationship in the range of 5–1500 mIU mL−1, with a detection limit of 1 mIU mL−1, the biosensor remained stable at room temperature for 14 days. Because of its small size, stability, sensitivity and accuracy, this biosensor has great potential to become a portable diagnostic device for HCG.
研究领域:
生物化学
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生物传感器
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分析化学
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妊娠诊断
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肿瘤学
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产品关联
DOI
PubMed
高影响因子
ACS Sensors
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2022
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IF 9.618
Xinyu He,Xiaoli Wang,Chuang Ge,Shunbo Li,Li Wang,Yi Xu
For the rapid and sensitive detection of vascular endothelial growth factor 165 (VEGF165) in clinical blood samples, a microfluidic sensing chip that integrates a centrifugal separation pretreatment unit and a composite nanosensing film was proposed in this paper. An efficient sensing strategy and method was established. The blood sample was first separated and extracted by centrifugal force on the centrifugal microfluidic chip within 5 min after injection. The separated plasma can be automatically transferred through the designed microchannels to the detection area integrated electrodes for subsequent differential pulse voltammetric detection. The Au NPs/MCH/Apt2 sensing film was constructed on the surface of the Au working electrode. A sandwich sensing strategy based on “double aptamers” and “nanoprobe” for VEGF165 detection was established, by which the synthetic Apt1/PThi/Au NP nanoprobe was applied to capture VEGF165 in plasma and bind to the sensing film. By this method, the detection limit of VEGF165 in whole blood was 0.67 pg/mL and the linear range was between 1 pg and 10 ng, which met the needs of clinical VEGF165 detection. It was illustrated that the proposed methodology based on the centrifugal microfluidic chip had potential application prospects in the development of the point-of-care testing fields.
研究领域:
生物医学工程
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分析化学
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即时诊断
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微流控技术
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生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）