Pathogens
·
2021
·Vol. 11
·期 1
·7
·
IF 3.492
Fuchun Zhang,Lingling Sun,Mark K. Lafferty,Joseph B. Margolick,Alfredo Garzino-Demo
CD4+CCR6+T cells are highly susceptible to HIV infection, and a high cytokine producing CCR6+T cell subset is selectively lost during HIV infection. The CCR6 chemokine MIP-3α (CCL20) is produced at sites of infection in SIV animal models. Recently, we have shown that MIP-3α inhibits HIV replication. This inhibition of HIV infection is mediated by CCR6 signaling and eventuates in increased APOBEC3G expression. Since there are few existing reports on the role of MIP-3α in health or disease, we studied its production by PBMCs from HIV-seronegative and HIV+ subjects. We evaluated the ability of PBMCs to produce MIP-3α in response to antigen stimulation using cells obtained from two groups: one composed of HIV-seronegative subjects (n = 16) and the other composed of HIV+ subjects (n = 58), some asymptomatic and some with clinically defined AIDS. Antigens included fragment C of the tetanus toxin,Candida albicans, whole-inactivated HIV, and HIV p24. MIP-3α was detected by ELISA in tissue culture supernatants of antigen-stimulated PBMCs. MIP-3α production by antigen-stimulated PBMCs was readily measured for HIV-negative subjects and for HIV-seropositive asymptomatic subjects, but not for patients with AIDS. These results suggest that subversion of the MIP-3α-CCR6 axis by HIV during the course of infection contributes to the loss of immune function that eventually leads to AIDS.Keywords:CCR6;MIP-3α;immune response;antigen stimulation;Th17
研究领域:
免疫学
·
病毒学
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DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2024
·Vol. 141
·期 16
·e55253
·
IF 2.7
Xiaocan Zhang,Siqin Zeng,Jiashu Long,Xuesong Li,Yutong Zhao
Using porous materials to selectively adsorb H 2 O from natural gas is a promising method to dehydrate methane (CH 4 ). The structure of a Zirconium metal-organic framework (Zr-MOF), namely, MIP-202, features a high density of amino groups, which can produce strong hydrogen bonding with H 2 O molecules. MIP-202 is cost-effective, green and scalable, and it exhibits good chemical stability under various conditions. This work incorporated MIP-202 nanoparticles into the high permeability polymer polyetherimide (PEI), and the obtained Mixed Matrix Membranes (MMMs) was used for the gas separation of H 2 O/CH 4 . It is found that MMMs loaded with 20 wt% of MIP-202 demonstrated a high H 2 O/CH 4 selectivity of 832.2, which was about 760 times of that of the pure PEI. This approach offers a new route to fabricate functional membranes for energy-efficient gas separations.
研究领域:
材料科学
·
环境工程
·
化学工程
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Jiaxin Liu,Jia Zheng,Yunshu Lu,Zhongmin Feng,Siqi Zhang,Ting Sun
17β-E 2 is used in animal growth regulation and agricultural fertilizer, and even ng L −1 mass concentration levels can show biological effects. In this work, Ag NPs was used as surface-enhanced Raman spectroscopy (SERS) source and WS 2 was synthesized by a simple method to provide a uniform distribution platform for Ag NPs. The MIP was the shell, which can selectively enrich the target molecule, pull the distance between the target molecule and SERS source, and protect Ag NPs. A cyclable SERS substrate with high sensitivity for detecting 17β-E 2 in food was constructed. The optimized WS 2 /Ag@MIP as SERS substrate has the advantages of high Enhanced Factor (EF = 2.78 × 10 9 ), low detection limit (LOD = 0. 0958 pM), strong anti-interference ability, and good recycling performance. Moreover, the detection of 17β-E 2 in real samples still has good accuracy. This work provides a new possibility for the trace detection of 17β-E 2 in food.
研究领域:
食品安全
·
分析化学
·
纳米材料科学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Haiyuan Chi,Chengjun Li,Mei Huang,Jinquan Wan,Xiaoxia Zhou,Bing Yan
Sulfate radicals-based advanced oxidation processes are regarded as effective methods for removing organic contaminants. However, current removal technologies are often unable to achieve satisfactory results due to the low concentrations and high stability that are frequently characteristics for many types of organic contaminants. To improve the efficacy of using sulfate radicals to remove organic contaminants, we designed metal–organic frameworks (Fe(II)-MOFs) modified with enrichment and specific recognition of molecular imprinting layer (Fe(II)-MOFs@MIP). Using dibutyl phthalate (DBP) as the model target contaminant, the selectivity coefficient towards DBP was 7.28 for adsorption and 4.46 for catalytic degradation of Fe(II)-MOFs@MIP, which is excellent for most of the imprinted material. Additionally, we achieved the accurate recognition (169.25 μg × g −1 ) and efficient degradation (0.071 min −1 ) of DBP reducing levels to 100 μg × L -1 . UV–vis analysis confirmed that specific recognition of DBP was mainly through hydrogen bonding and π-π interaction, while in-situ Raman and radical quenching experiments showed that the imprinting layer could accurately adsorb and produce the spatial confinement effects on target contaminants, thereby reducing the transfer distance of free radicals. As a result, we show that Fe(II)-MOFs@MIP can achieve efficient targeted degradation of DBP, opening up a new avenue for the removal of this and other highly stable organic contaminants present at low concentrations in environmental systems.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Suqi Liu,Xiaowei Wu,Shihao Chen,Cong Liu,Wanqi Zhang,Mingyi Yang,Jiyu Cheng,Guoquan Lu,Zhenhe Wang,Wei Chen,Yuge Guan,Linjiang Pang,Xinghua Lu
Linalool, a terpenoid alcohol with bacteriostatic properties, was detected in sweetpotato suffering from black spot disease. In this research, a composite material based on HKUST-1/MWCNT-gel@MIP was employed to detect the linalool content in these infected sweetpotatoes. The micro-morphology and crystal structure of the composite were characterized using scanning electron microscopy (SEM), fourier transform infrared (FTIR), and x-ray diffraction (XRD). Experimental results demonstrated that the quartz crystal microbalance (QCM) gas sensor of HKUST-1/MWCNT-gel@MIP composite exhibited high sensitivity (0.873 Hz/ppm), a low detection limit (0.237 ppm), a small standard deviation (0.83 %), good reproducibility, and outstanding selectivity. The QCM gas sensor data from actual samples showed a strong correlation with gas chromatography-mass spectrometry (GC–MS) results (r = −0.976, p < 0.01), demonstrating its exceptional ability to identify linalool. Thus, it can be used for early detection of sweetpotato black spot disease.
研究领域:
植物病理学
·
分析化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Mingcai Yao,Liang Dong,YuHang Fu,Haijun Li,Guang Luo
We have carefully built a new chloramphenicol (CAP) electrochemical sensor, which takes the zinc tungstate @ cobalt magnetic nanoporous carbon @ molecularly imprinted polymer (ZnWO 4 @Co-MNPC@MIP) as the core. First, we successfully prepared Co-MNPC nanomaterials using an efficient one-step hydrothermal method and a direct carbonization method. Next, we recombined ZnWO 4 with Co-MNPC and synthesized the completely new ZnWO 4 @Co-MNPC complex by using the hydrothermal method. To further improve its performance, we combined ZnWO 4 @ Co -MNPC with a molecular imprinted polymer and coated a molecular imprinted (MIP) shell on the surface of ZnWO 4 @ Co -MNPC by precipitation polymerization. This shell not only gives the sensor a new performance but also gives it a stronger peak current, resulting in a more accurate detection of CAP. Under optimal conditions, the ZnWO 4 @Co-MNPC@MIP (MMIP) electrode has a stronger CAP detection peak current than the one-component electrode, with a fairly wide linear range: 0.007–200 μM and 200–1400 μM. Even more surprisingly, the detection limit is as low as 0.0027 μM, which allows the sensor to maintain excellent selectivity and stability in the face of various interferences, making it an excellent electrochemically modified electrode. Compared to magnetic non-molecular imprint sensors (MNIPs), MMIP sensors have higher detection efficiency. After practical application, we found that the ZnWO 4 @Co-MNPC@MIP modified electrode was satisfactory in milk samples.
研究领域:
分析化学
·
纳米技术
·
食品安全
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Han Li,Donghui Wang,Wei Liu,Zhan Ma,Dingnan Wang,Yang Li,Weiwei Huan,Yiming Zhang
The development of high-performance specific sensors is promising for the rapid detection of harmful residues in animal-derived foods. Recently, luminescent metal-organic framework/molecularly imprinted polymer (LMOF/MIP) materials have been developed as ideal candidates for the analysis of harmful residues. Here, we reported a simple fabrication protocol of paper-based chip through in-situ growth of LMOF on a negatively charged modified filter paper, a paper-based molecularly imprinting layer (FP@BA-Eu@MIP) was thereafter successfully prepared via the boronate affinity-based controllable oriented surface imprinting strategy. The paper-based chips obtained were used to construct a rapid test strip of tetracycline (TC). After addition of TC, significant fluorescence changes on the surface of the FP@BA-Eu@MIP paper-based chip could be observed from blue to red via inner filter effect and photo-induced electron transfer under the excitation of 360 nm. The adsorption kinetics was explored in detail. The presented strip exhibited satisfied selectiveness and sensitivity with a limit of detection of 8.47 μg L −1 for TC. It was confirmed that LMOF/MIP as a biomimetic recognition module can play a crucial role in enrichment and fluorescence response. This study provided a real application case for an in-situ fabricated fluorescence paper-based chip in rapidly detecting harmful residues.
研究领域:
分析化学
·
食品科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2020
·Vol. 10
·期 9
·1655
·
IF 4.324
Yuqiong Shi,Yuxuan Wang,Jinhua Zhu,Wei Liu,Md. Zaved H. Khan,Xiuhua Liu
MIL-based molecularly imprinted polymer (MIP) nanocomposites were successfully synthesized through a simple and versatile stirring auxiliary encapsulation method. MIP as a carrier has been applied to the highly efficient selective recognition and sustained release of doxorubicin hydrochloride (DOX). The adsorption mechanism and release behavior of[email protected]in vitro were also discussed. Adsorption studies showed that MIP using DOX as template had specific selectivity to DOX, and its optimal drug loading efficiency reached 97.99%. The adsorption isotherm accorded with Freundlich models. The cumulative release curve showed that at the conditions of pH 5.5 and 7.4, the nanomaterials have a slow-release effect on the release of DOX. In addition, the cytotoxicity and bioactivity of MIP nanoparticles on HepG2 and HL-7702 cell lines measured by MTT assay also proved their low toxicity and biological activity. The cell activity of HepG2 and HL-7702 incubated with MIP for 24 h was 69.9% and 76.07%, respectively. These results collectively illustrated that the MIP nano-materials synthesized in this study can be efficiently employed to the drug delivery systems.Keywords:MIP;nanocomposites;drug delivery;DOX;controlled release
研究领域:
材料科学
·
药学
·
纳米技术
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 7
·2241-2248
·
IF 4.58
Zhang Yufeng,Zhou Zhiping,Zheng Jiahong,Li Hongji,Cui Jiuyun,Liu Siwei,Yan Yongsheng,Li Chunxiang
The authors describe a fluorescence based assay for determination of the macrolid antibiotic erythromycin (ERY). It is based in the use of fluorescent gold nanoclusters (AuNCs) coated first with silica (to obtain AuNC@SiO 2 ) and then with a molecularly imprinted polymer (MIP). The MIP was synthesized from 3-aminopropyltriethoxysilane as the functional monomer, ERY as the template, and tetraethoxysilane as the cross-linker via a sol-gel process that leads to surface imprinting. By using this method, the strong fluorescence of AuNCs was maintained in the resultant MIPs. After optimization of the experimental conditions, the signal (measured at excitation/emission wavelengths of 396/585 nm) decreases linearly with increasing concentration of ERY in the 0.1 to 11.9 μM ERY concentration range. The limit of detection is 12 nM, and the imprinting factor is 4.0. The method was successfuly applied to the determination of ERY in spiked human urine and saliva.
Schematic of the preparation of a core-shell sensor material with the silica coated by fluorescent gold nanoclusters as core and a molecularly imprinted polymer. It can be used to selectively detect residual erythromycin in biological samples.
研究领域:
分析化学
·
纳米技术
·
生物传感器
·
药物分析
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2015
·
IF 4.321
Jie-Ping Fan,Xiao-Kang Xu,Rui Xu,Xue-Hong Zhang,Jian-Hang Zhu
In this work, the superparamagnetic Fe 3 O 4 particles prepared by the coprecipitation method were coated successively by SiO 2 , vinyl end groups and molecularly imprinted polymer (MIP) shells to afford a pomegranate-like core/shell superparamagnetic particle (Fe 3 O 4 @SiO 2 @MIP), and the MIP shell preparation conditions were optimized by multi-objective response surface methodology. In order to simultaneously enrich and separate the four taxoids (10-DAB, baccatin III, cephalomannine and paclitaxel), cephalomannine was selected as the template because of its similar chemical structure to other three taxoids and relative inexpensiveness. Especially, to avoid template leaking which may influence the accuracy of identification and quantitation of the analytes, we selected paclitaxel as the target molecular during the investigation of the adsorption property of Fe 3 O 4 @SiO 2 @MIP particles. This strategy was similar to the dummy molecular imprinting technique in which the dummy molecularly imprinted polymer was prepared by using a dummy template, structural analog of the target analyte. The as-prepared Fe 3 O 4 @SiO 2 @MIP particles were characterized by transmission electron microscopy (TEM), vibrating sample magnetometer (VSM), and Fourier transform infrared spectroscopy (FTIR). The adsorption kinetics, isotherms and reusability were also investigated. Finally, the Fe 3 O 4 @SiO 2 @MIP particles were successfully applied to simultaneously enrich and separate these four taxoids in the extract of Taxus × media ( T. × media ).
研究领域:
分析化学
·
材料科学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
PROCESS BIOCHEMISTRY
·
2018
·
IF 2.616
Qi Liu,Junfen Wan,Xuejun Cao
Spiramycin I is a main component of spiramycin and a structural analogue of neospiramycin I, which is an impurity in spiramycin production. Molecular imprinting technology (MIT) has many advantages in selectivity and environmental compatibility in biomolecular separation. In this study, core-shell molecularly imprinted polymers (MIPs) were prepared for spiramycin I. Methyl methacrylate (MMA) was used to prepare the seed core, and acrylamide (AM)/methacrylic acid (MAA) was chosen to prepare the shell. The structure of polymerized MIPs was successfully characterized by transmission electron microscopy, dynamic light scattering, Fourier transform infrared spectroscopy and elemental analysis. The adsorption results showed that MIPs polymerized by different monomers exhibited very different adsorption for spiramycin I. MIPs with monomeric AM achieved a fast mass transfer, high adsorption capacity and high selectivity. Then, these MIPs were applied to separate spiramycin I from the fermentation broth in the chromatographic column, and its recovery and purity were 61% and 83%, respectively. Finally, regeneration of the MIP chromatographic column was studied to explore its recycling performance in application.
研究领域:
高分子化学
·
分析化学
·
分离科学
·
制药技术
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DOI
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2022
·
IF 3.898
Daofei Lv,Junhao Xu,Pingjun Zhou,Shi Tu,Feng Xu,Jian Yan,Hongxia Xi,Zewei Liu,Wenbing Yuan,Qiang Fu,Xin Chen,Qibin Xia
The separation of propylene and propane is an important but challenging process, primarily achieved through energy-intensive distillation technology in the petrochemical industry. Here, we reported two natural C 4 linkers based metal–organic frameworks (MIP-202 and MIP-203) for C 3 H 6 /C 3 H 8 separation. Adsorption isotherms and selectivity calculations were performed to study the adsorption performance for C 3 H 6 /C 3 H 8 separation. Results show that C 3 H 6 /C 3 H 8 uptake ratios (298 K, 100 kPa) for MIP-202 and MIP-203 are 2.34 and 7.4, respectively. C 3 H 6 /C 3 H 8 uptake ratio (303 K, 100 kPa) for MIP-203 is up to 50.0. The mechanism for enhanced separation performance of C 3 H 6 /C 3 H 8 on MIP-203 at higher temperature (303 K) was revealed by the in situ PXRD characterization. The adsorption selectivities of C 3 H 6 /C 3 H 8 on MIP-202 and MIP-203 (298 K, 100 kPa) are 8.8 and 551.4, respectively. The mechanism for the preferential adsorption of C 3 H 6 over C 3 H 8 in MIP-202 and MIP-203 was revealed by the Monte Carlo simulation. The cost of organic ligands for MIP-202 and MIP-203 was lower than that of organic ligands for those top-performance MOFs. Our work sets a new benchmark for C 3 H 6 sorbents with high adsorption selectivities.
研究领域:
化学工程
·
材料科学
·
石油化工
✓ 已验证
DOI
高影响因子
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