Journal of Materials Chemistry B
·
2014
·Vol. 2
·期 13
·1825-1837
·
IF 0
Changcan Shi,Fanglian Yao,Jiawen Huang,Guoliang Han,Qian Li,Musammir Khan,Yakai Feng,Wencheng Zhang
Herein, we developed a novel biodegradable gene carrier for rapid endothelialization of endothelial cells (ECs) in vitro. Three triblock amphiphilic copolymers, methoxy-poly(ethylene glycol)-block-poly(3(S)-methyl-2,5-morpholinedione-co-glycolide)-graft-polyethyleneimine (mPEG-b-P(MMD-co-GA)-g-PEI) with different 3(S)-methyl-2,5-morpholinedione and glycolide contents were synthesized. Microparticles (MPs) were obtained via self-assembly of these copolymers. The hydrophobic core composed of P(MMD-co-GA) segments provide crosslinking points for numbers of PEG and short PEI chains to form a highly hydrophilic and positively charged corona/shell of MPs. Using these MPs, potential genes (ZNF580) for rapid endothelialization were efficiently transported into EA.hy926 cells. Because of the hydrophilic PEG chains and low molecular weight PEI in the triblock copolymers, the cytotoxicity of these MPs and their complexes with pEGFP–ZNF580 was decreased significantly. The transfection efficacy of MPs/pEGFP–ZNF580 complexes was as high as Lipofectamine™ 2000 reagent to EA.hy926 cells in vitro. The proliferation and migration of EA.hy926 cells were improved greatly by the expression of pEGFP–ZNF580 after 60 hours. Our results indicated that the mPEG-b-P(MMD-co-GA)-g-PEI based MPs could be a suitable non-viral gene carrier for ZNF580 gene to enhance rapid endothelialization.
研究领域:
生物医学工程
·
基因治疗
·
材料科学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
FOOD CONTROL
·
2026
·
IF 7
Zhenbin Liu,Jia Luo,Jiayi Zhang,Rui Weng,Wenchao Liu,Hongbo Li,Huan Cheng,Dong Wang,Hengchao E,Lu Tian,Haizhen Mo,Liangbin Hu
Our previous studies have demonstrated that multiphase microwave drying (MMD) can simultaneously achieve high drying efficiency and superior product quality. This study further investigated the quality formation mechanism during the MMD process. Results indicated that with increasing drying time, free and bound water were progressively removed, resulting in the formation of a uniform and well-connected porous structure and a marked improvement in rehydration capacity. Micro-CT and TEM analyses confirmed that MMD enabled efficient dehydration while largely preserving cell wall integrity. FTIR and DSC results indicated that the secondary structure and thermal stability of mushroom proteins remained generally stable throughout the drying process. Texture analysis together with LF-NMR and Micro-CT results indicated that drying for approximately 90 min achieved the best balance between structural stability and rehydrated texture, as reflected by the formation of a well-connected porous network, enhanced rehydration capacity, and coordinated hardness and springiness. Metabolomic analysis further revealed that drying time primarily regulated amino acid, lipid, and carbohydrate metabolic pathways, thereby promoting the formation of flavor precursor compounds. Overall, this study elucidates the intrinsic relationships among moisture migration, structural evolution, and quality formation during MMD, providing a theoretical basis for parameter optimization and high-quality processing of shiitake mushrooms.
✓ 已验证
DOI
高影响因子
TRANSACTIONS OF TIANJIN UNIVERSITY
·
2012
·Vol. 18
·期 5
·315-319
·
IF 0
Feng Yakai,Chen Chengbin,Zhang Li,Tian Hong,Yuan Wenjie
A series of novel copolymers were successfully synthesized by ring-opening polymerization (ROP) of 3(S)-methyl-morpholine-2,5-dione (MMD) and 5-methyl-5-benzyloxycarbonyl-1,3-dioxan-2-one (MBC) using stannous octoate as catalyst. The copolymers were characterized by means of 1H-NMR and FT-IR spectroscopy. Gel permeation chromatography (GPC) test shows that the average-number relative molecular mass and average-weight relative molecular mass slightly increase with the increase of MBC content in feed. The results of differential scanning calorimetry (DSC) demonstrate that the glass transition temperature of copolymers increases with the increase of MBC content in copolymers. The copolymers of MMD and MBC are amorphous copolymers, as indicated by DSC results, while the homopolymer of MMD is semicrystalline.
研究领域:
高分子化学
·
材料科学
✓ 已验证
产品关联
DOI
Food and Bioprocess Technology
·
2025
·
IF 5.8
Liu Zhenbin,Luo Jia,Zhang Jiayi,Liu Wenchao,Li Hongbo,Hu Liangbin,Wang Dong,Hengchao E,Sun Zhenying,Xia Yu,Weng Rui,Mo Haizhen
Multi-phase microwave drying (MMD) uses microwaves as a heat source while adjusting chamber pressure to dry materials, enabling solid-liquid-gas phase transitions of water. This method significantly shortens drying time and reduces energy consumption. This study explores MMD’s feasibility as an efficient and high-quality drying technique for shiitake mushrooms, comparing it with hot air drying (HAD), heat pump drying (HPD), freeze drying (FD), and microwave vacuum drying (MVD). The results show MMD dries mushrooms in 105 minutes, achieving speeds 5, 7, and 13 times faster than HAD, HPD, and FD, respectively. MMD also significantly reduces shrinkage (14.0%) and improves rehydration (519.4%), surpassing HAD, HPD, and MVD by 66.79%, 60.99%, and 269.53%. Additionally, MMD better preserves volatile compounds and the mushrooms’ original flavor, reducing losses due to overheating. Nutritionally, MMD retains higher levels of amino acids (34.92 mg/g) and nucleotides (160.31 mg/100 g), boosting umami flavor (786.96 g MSG/100 g). This method combines microwave drying’s efficiency with freeze drying’s quality benefits.
研究领域:
食品科学
·
农产品加工
·
干燥技术
·
食品质量
·
食品营养
✓ 已验证
产品关联
DOI
高影响因子
BIOMATERIALS
·
2021
·
IF 12.479
Xianzhen Dong,Ping Wu,Lesan Yan,Kun Liu,Wenying Wei,Qiang Cheng,Xinyue Liang,Yun Chen,Honglian Dai
Delayed injured nerve regeneration remains a clinical problem, partly ascribing to the lack of regulation of regenerative microenvironment , topographical cues, and blood nourishment. Functional electrospun conduits have been established as an efficacious strategy to facilitate nerve regeneration by providing structural guidance, regulating the regenerative immune microenvironment, and improving vascular regeneration. However, the synthetic polymers conventionally used to fabricate electrospinning scaffolds, such as poly(L-lactic acid), poly(glycolic acid), and poly(lactic-co-glycolic acid), can cause aseptic inflammation due to acidic degradation products . Therefore, a poly[3(S)-methyl-morpholine-2,5-dione- co -lactic] [P(MMD- co -LA)] containing alanine units with good mechanical properties and reduced acid degradation products, was obtained by melt ring-opening polymerization (ROP). Here, we aimed to explore the effect of oriented nanofiber/Deferoxamine (DFO, a hydrophilic angiogenic drug) scaffold in the rapid construction of a favorable regenerative microenvironment, including cell bridge, polarized vascular system, and immune microenvironment. In vitro studies have shown that the scaffold can sustainably release DFO, which accelerates the migration and tube formation of human umbilical vein endothelial cells (HUVECs), as well as the expression of genes related to angiogenesis . The physical clues provided by the arranged nanofibers can regulate the polarization of macrophages and reduce the expression of inflammatory factors. Furthermore, the in vivo results demonstrated a higher M2 polarization level of the oriented nanofibrous scaffold treatment group with reducedinflammation reaction in the injured nerve. Moreover, the in-situ release of DFO up-regulated the expression of HIF1-α and SDF-1α genes, as well as the expression of HIF1-α′s target gene VEGF, further promoting revascularization and enhancing nerve regeneration at the defect site . The obtained results provide essential insights on accelerating the creation of the nerve regeneration microenvironment by combining the physiological processes of nerve regeneration with topographical cues and chemical signal induction.
研究领域:
生物材料
·
组织工程
·
再生医学
·
神经科学
·
免疫学
·
血管生成
✓ 已验证
DOI
PubMed
高影响因子
Materials Today Bio
·
2022
·
IF 10.761
Lei Han,Xianzhen Dong,Tong Qiu,Zhaona Dou,Lin Wu,Honglian Dai
Wallerian degeneration after peripheral nerve injury (PNI), that is, the autonomous degeneration of distal axons, leads to an imbalance of iron homeostasis and easily induces oxidative stress caused by iron overload. Inspired by the process of nerve degeneration and regeneration, the design of a functional electrospinning scaffold with iron chelating ability exhibited the importance of reconstructing a suitable microenvironment. Here, an electrospinning scaffold based on deferoxamine and poly(3(S)-methyl-morpholine-2,5-dione -co- lactone) (PDPLA/DFO) was constructed. This work aims to explore the promotion of nerve regeneration by the physiological regulation of the scaffold. In vitro , PDPLA/DFO films mitigated the reduction of glutathione and the inactivation of Glutathione peroxidase 4 caused by iron overload. In addition, they decreased reactive oxygen species, relieve the stress of the endoplasmic reticulum and mitochondria, and reduce cell apoptosis. In vivo , PDPLA/DFO conduits constructed the anti-inflammatory microenvironment and promoted cell survival by alleviating iron overload and organelle stress. In conclusion, PDPLA/DFO guidance conduits targeted the distal iron overload and promoted nerve regeneration. It provides novel ideas for designing nerve conduits targeting the distal microenvironment.
研究领域:
神经科学
·
材料科学
·
再生医学
✓ 已验证
DOI
PubMed
高影响因子
Advanced Materials Interfaces
·
2023
·
IF 5.4
Weixing Wang,Yifeng Yu,Hao Zhang,Zhiwei Wang,Honglian Dai,Aixi Yu
To solve the problem of tendon adhesion after an operation, an anti-adhesion membrane is designed, which can inhibit the exogenous healing of tendons and promote endogenous healing. Here, poly[3(S)-methyl-morpholine-2,5-dione- co -lactic acid] P(MMD- co -LA) containing alanine units is obtained by melt ring-opening polymerization (ROP). It can effectively reduce the production of acidic degradation products that cause aseptic inflammation. Then a kind of oriented P(MMD- co -LA)/deferoxamine (DFO) anti-adhesion membrane with good mechanical properties, cell adhesion properties, and induced macrophage polarization properties is constructed by electrospinning. In vitro and in vivo studies have shown that oriented fibrous membranes can down-regulate the expression of inflammatory cytokines. In addition, the fibrous membrane can up-regulate the expression of angiogenesis-related genes, namely HIF1-α, SDF-1α, and VEGF, by releasing DFO in situ, thus promoting the revascularization of tendon defects and providing nutritional support for endogenous tendon healing. This method provides a new barrier strategy to reduce tendon adhesion through anti-inflammation combined with vascularization to inhibit exogenous tendon healing while promoting tendon endogenous healing. The results provide essential insights into the corresponding regulation of the tendon healing microenvironment.
研究领域:
生物医学工程
·
组织工程
·
材料科学
·
再生医学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）