MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Qianshun Chen,Qiuyan He,Yiting Wang,Chen Huang,Yuyu Lin,Jieyang Wang,Wei Shen,Bin Qiu,Xunyu Xu
Carcinoembryonic antigen (CEA) is the most widely adopted biomarker for the early determination of various cancers, thus it is very important to design a simple and highly effective sensing method to detect CEA. Herein, by synthesizing Fe-MOF nanohybrid loaded with platinum nanoparticles (Pt NPs/Fe-MOF) nanozyme and selecting CEA aptamer (Apt) as the specific recognition element, a simple nanozyme-based voltammetric sensor was designed to detect CEA based on Apt-controlled the catalytic activity of Pt NPs/Fe-MOF nanozyme. The as-synthesized Pt NPs/Fe-MOF nanozyme could catalyze effectively 1,2-diaminobenzene oxidation to produce electroactive 2,3-diaminophenazinc (DAP) which can exhibit a strong reduction current. Nonetheless, when Pt NPs/Fe-MOF is covered fully by Apt of CEA, the peroxidase activity of the as-synthesized Pt NPs/Fe-MOF nanozyme is remarkably restricted and the peak current of DAP would disappear nearly. Interestingly, this catalytic activity could be recovered effectively in the presence of CEA owing to the specific identification of Apt toward CEA. Through optimizing several experimental factors, the as-designed nanozyme voltammetric sensor offers superior detective performance for CEA. In addition, the as-designed sensor possesses superior specificity and selectivity, reproducibility and stability as well as practical applications, showing great potentials for achieving simple, sensitive and highly effective determination of CEA.
研究领域:
生物化学
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医学诊断
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纳米技术
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 6
·1-11
·
IF 6.408
Guo Jingjing,Liu Yangyang,Mu Zhao,Wu Shu,Wang Jueyu,Yang Yue,Zhao Min,Wang Yan
A bifunctional Ni-MOF nanosheet was synthesized and developed for label-free fluorescent detection of H 2 O 2 and glucose. The Ni-MOF exhibited intrinsic peroxidase-like activity and its catalytic activity was demonstrated to be originated from the hydroxyl radicals (•OH) produced in catalytic process. Since the generated •OH enabled terephthalic acid, the non-fluorescent organic ligand of Ni-MOF, to form a strongly fluorescent 2-hydroxy terephthalic acid, the Ni-MOF nanozyme was endowed with dual-function properties of mimicking peroxidase and emitting fluorescence. Based on this bifunctional Ni-MOF nanozyme, the proposed label-free fluorescence sensing strategy was applied to detecting H 2 O 2 and glucose with wide linear ranges of 0.1–20 mM and 8–30 μM, and low detection limits of 4.0 × 10 −5 M and 4.0 × 10 −6 M, respectively. Furthermore, the bifunctional Ni-MOF-based label-free sensing platform was successfully used for the glucose detection in human serum samples, showing good reproducibility and high accuracy. This strategy provides a green and sensitive method for the determination of small biomolecules in practical applications by the combination of enzyme cascade reaction. Graphical abstract
研究领域:
生物化学
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分析化学
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材料科学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Zijing Xu,Dalan Jin,Xiaoyi Lu,Yonglun Zhang,Weilu Liu
Ovalbumin (OVA) is the main allergen in vaccines, and the sensitive detection of it could protect hypersensitive people. However, traditional detection technologies usually require natural antibodies, natural enzymes and long analysis time, which is not conducive to transportation, preservation and rapid detection. To solve this problem, the sandwich colorimetric strategy using Ni-Fe-MOF nanozyme linked with magnetic silicon dioxide surface molecularly imprinted particles (MSi-MIPs) are proposed for the first time to rapidly detect OVA in vaccines. The high sensitivity of this strategy depends on the sandwich structure of surface molecularly imprinted nanoparticles, OVA and nanozymes. Ultra-thin Ni-Fe-MOF nanozyme exhibits large specific surface area and more peroxidase active sites. This has enabled the establishment of a specific and stable detection tool for OVA in vaccines. Under the optimal conditions, the assay exhibited a wide linear response range of 2.5 to 25 ng mL −1 . The low limit of detection (1.02 ng mL −1 ) and the short analysis time (20 min) demonstrated the great potential of the strategy for OVA detection in vaccine samples.
研究领域:
生物化学
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免疫学
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材料科学
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产品关联
DOI
高影响因子
Nano Today
·
2025
·
IF 10.9
Mingkai Wang,Ruiyang Li,Shihao Sheng,Huijian Yang,Xuan Tang,Jian Wang,Fuxiao Wang,Qin Zhang,Long Bai,Xiao Chen,Jie Gao,Xiaoxiang Ren,Han Liu,Jiacan Su
The rising prevalence of multidrug-resistant pathogens poses a significant threat to human health, with methicillin-resistant Staphylococcus aureus (MRSA) being particularly concerning. Although vancomycin has long been the standard treatment for MRSA, its repeated use can lead to antibiotic tolerance and resistance. The accumulation of bacterial metabolites contributes to this tolerance, suggesting that modulation of bacterial metabolic pathways may provide a novel solution. Herein, we developed Ga/Cu-MOF nanomyze, characterized by a high specific surface area, which could be capable of loading antibiotics and serve as a dual-metal ion reservoir for the sustained release of gallium ions (Ga 3 + ) and copper ions (Cu 2+ ). These ions independently inhibited nitric oxide (NO) production and promoted the decomposition of S-nitrosothiols (RSNO), significantly reducing MRSA's tolerance to vancomycin. Moreover, Ga/Cu-MOF induced programmed cell death in MRSA, specifically targeting ferroptosis and cuproptosis. Antibiotic-loaded nanomyze exhibited robust synergistic antibacterial properties, including the inhibition of biofilm formation and the clearance of intracellular bacteria in macrophages. In preclinical models of skin defect infections in mice and osteomyelitis in rats, Ga/Cu-MOF showed the ability to regulate the host inflammatory response, facilitating faster recovery. Our goal is to reduce bacterial tolerance by modulating metabolic pathways and enhance antibacterial efficacy through bacterial cell death, thereby presenting a potential novel therapeutic strategy for the clinical management of multidrug-resistant bacteria.
研究领域:
微生物学
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药理学
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纳米医学
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产品关联
DOI
高影响因子
Food Chemistry-X
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2025
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IF 8.2
Xiang-Yi He,Ya Wang,Qin Xue,Wan-Fen Qian,Guang-Li Li,Qing Li
Reliable and accurate determination of chloramphenicol (CAP) is urgently needed due to its significant implications for food safety and human health concerns. In this study, we successfully synthesized MIL-101(Fe)-NH 2 @MIP, which exhibits peroxidase activity and a specific recognition function for CAP, through in situ polymerization of dopamine on the surface of MIL-101(Fe)-NH 2 , utilizing molecular imprinting technology. Our bifunctional MIL-101(Fe)-NH 2 @MIP probe offers a ratiometric fluorescent and colorimetric dual-mode sensing strategy for sensitive and selective detection of chloramphenicol. The detection limits for CAP can reach down to 36.45 nM and 93.38 nM, respectively. Furthermore, we developed a smartphone-based visual sensing platform that employs MIL-101(Fe)-NH 2 @MIP nanozymes for rapid, portable, low-cost on-site detection of CAP. Successful spike recovery experiments conducted in fresh milk samples further validate the potential practical application of our proposed dual-mode strategy. Thus, this approach expands the applications of MOFs-based nanozymes and holds promise for antibiotic determination.
研究领域:
食品安全
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生物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
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IF 4.9
Changchang Li,Yue Zhi,Minnan Zhu,Chengyu Yang,Yayang Tian,Tao Chen,Yuan Zhao,Jie Gao,Linfeng Chen
The development of simple, efficient and low-cost methods for rapid on-site monitoring have always been a challenging topic in the field of analysis. Here, we successfully constructed a selective, sensitive and rapid colorimetric sensor for the sensing of diethylstilbestrol (DES) by using molecular imprinting technology to construct specific recognition sites on the surface of Fe-based metal organic frameworks (Fe-MOF) peroxidase-like nanozyme. The surface of obtained sensor (Fe-MOF@MIP) had abundant channels and imprinted cavities that allowed the substrate to enter and responded with Fe-MOF core to drive the chromogenic reaction of 3,3′,5,5′-tetramethylbenzidine (TMB). When DES was present, it could specifically enter the cavities and obstruct the channels of substrate into the Fe-MOF core, thereby inhibiting the chromogenic reaction. The experimental results demonstrate that the sensor had excellent selectivity, anti-interference and catalytic stability. In the linear range of 0 – 1.67 μ M, the detection limit is as low as 8.43 nM, which is superior to peer sensors. To better adapt to in-situ analysis, we have further developed a rapid analysis method based on photo colorimetry, which performed well in real sample analysis and recovery test with the results consistent with the gas chromatograph-mass spectrometer (GC–MS), demonstrating its great potential for rapid on-site monitoring.
研究领域:
分析化学
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生物医学工程
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环境科学
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DOI
高影响因子
CRYSTENGCOMM
·
2022
·Vol. 25
·期 23
·3472-3483
·
IF 3.756
Yiqian Su,Hongjiao Wu,Jiaqi Chen,Huiqin Li,Pengcheng Lin,Wei Xiao,Donglin Cao
Emerging peroxidase (POD)-like nanozymes have been exploited to construct biosensors towards biomolecular detection. However, the enzymatic activity, sensing mechanism and detection method of the biosensors towards ascorbic acid (AA) analysis are still challenging research topics. Herein, a novel scandium-MOF (Sc-MOF) nanozyme is synthesized via a wet-chemical method. An efficient AA biosensor is developed owing to the POD-like characteristics of the Sc-MOF. The Sc-MOF nanozyme can convert hydrogen peroxide into hydroxyl radicals. Afterwards, colorless 3,3′,5,5′-tetramethylbenzidine (TMB) is converted into blue oxidized TMB by hydroxyl radicals. The Michaelis–Menten constants of the Sc-MOF nanozyme (Km = 0.013 mM for TMB and 0.246 mM for H2O2) are obviously superior to those of horseradish peroxidase. The AA concentration can be determined by putting forward a colorimetric method and an absorbance turn-off mechanism. The linear range of the Sc-MOF based colorimetric method is 0.2–20 μM. The limit of detection is 0.174 μM. The AA detection also demonstrates superior specificity and anti-interference features. Furthermore, the Sc-MOF-based biosensors are applied to qualitative and quantitative AA detection in human serum. The Sc-MOF nanozyme can inspire researchers to develop novel transition metal based biological enzymes and expand their applications in enzymatic biochemical reactions.
研究领域:
生物传感
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材料科学
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医学检测
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2025
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IF 9.8
Lu Gao,Qianwen Long,Baizhi Cen,Qi Gao,Mengyuan Tan,Ling Zhang,Juanli Yang,Yuan Ma,Xuechao Xu,Zhen-quan Yang
A chromogenic system utilizing a novel bacteriophage-integrated nanozyme was established for Proteus penneri detection. Initially, a novel bacteriophage PpZDSS02 was isolated and identified, demonstrating exceptional biological properties and harboring genes that exhibit specific recognition ability. Afterwards, a PpZDSS02-integrated Cu-MOF nanozyme (Cu-MOF@PpZDSS02) with peroxidase-mimicking activity was prepared, catalyzing 3, 3′, 5, 5′-tetramethylbenzidine (TMB) chromogenic reaction. Its peroxidase-mimicking activity can be modulated by P. penneri . Interestingly, low concentration of P. penneri can enhance its activity and high concentration of P. penneri inhibits its activity. Based on it, a visual quantification method with high sensitivity and specificity for P. penneri was achieved, offering a low limit of detection (3.3 CFU·mL −1 ). Moreover, Cu-MOF@PpZDSS02-based chromogenic system shows exceptional performance in the detection of authentic food samples, achieving ideal recoveries (89.00 % ∼ 111.81 %). These results indicate the potential application of Cu-MOF@PpZDSS02-based chromogenic system for colorimetric detection of P. penneri .
研究领域:
生物传感
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微生物学
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食品科学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2024
·
IF 10.7
Jingyu Hou,Jun Wang,Jiawen Han,Juan Wang,Daiyong Chao,Qing Dong,Daoqing Fan,Shaojun Dong
As the well-known test-indicator for early prostate cancer (PCa), sarcosine (SA) is closely related to the differential pathological process, which makes its accurate determination increasingly significant. Herein, we for the first time expanded the peroxidase (POD)-like property of facile-synthesized Zn-TCPP(Fe) MOF to fluorescent substrates and exploited it to ratiometric fluorescent (RF) sensing. By harnessing the effective catalytic oxidation of MOF nanozyme toward two fluorescent substrates (Scopoletin, SC; Amplex Red, AR) with contrary changes, and target-responsive (SA + SOx)/MOF/(SC + AR) tandem catalytic reaction, we constructed the first MOF nanozyme-based RF sensor for the quantitative determination of SA. Superior to previous works, the operation of this RF sensor is under the guidance of AND-(ANDˆNAND) contrary logic circuit. The dual-channel binary output changes (from 1/0 to 0/1) not only enable the intelligent logical recognition of SA, bringing strengthened reliability and accuracy, but also manifest the proof-of-concept discrimination of PCa individuals and healthy ones. Through recording the fluorescence alterations of SC (F 465 ) and AR (F 585 ), two segments of linear relationships between ratiometric values (F 585 /F 465 ) and varied contents of SA are realized successfully. The LOD for SA could reach to as low as 39.98 nM, which outperforms all nanozyme-originated SA sensors reported till now. Moreover, this sensor also demonstrates high selectivity and satisfactory performance in human serum samples. Furthermore, the portable sensing of SA is realized under the assistance of smartphone-based RGB analysis, demonstrating the potential of point-of-care diagnostics of PCa in the future.
研究领域:
生物技术
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分析化学
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癌症诊断
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纳米技术
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生物化学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Ziyan Chen,Shen Song,Huajing Zeng,Zhenlin Ge,Bin Liu,Zengjie Fan
Promoting rapid healing of diabetic wounds caused by hyperglycemia, bacterial infection, and chronic inflammation is a global challenge. To address this issue, we design and prepare a novel cerium-based MOF nanozyme hydrogel via 3D printing technology to provide a personalized hydrogel wound dressing. The hydrogel is unique in that cerium-based MOFs are grown into the hydrogel network, simplifying the printing process of MOF hydrogel. The prepared hydrogel exhibits specific catalytic activity to various oxygen free radicals and glucose concentration-dependent color changes due to the interconversion between different valence cerium ions. This feature allows for indirect monitoring of glucose content around the wound. Additionally, the hydrogel presented high antibacterial properties and biocompatibility. In animal experiments, diabetic wounds in rats with a size of 1 cm 2 fully healed within 21 days due to the synergistic effect of the anti-inflammatory and hyperglycemia capabilities of the cerium-based MOF nanozyme hydrogel. The results suggest that this novel hydrogel will revolutionize traditional treatment methods in diabetic wounds and provide an efficient, rapid, personalized treatment program.
研究领域:
材料科学
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生物医学工程
·
纳米技术
·
伤口愈合
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糖尿病研究
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 2
·1-12
·
IF 5.3
Ma Shuyan,Wei Chenjie,Bao Yuheng,Liu Yanhui,Jiang Hong,Tong Weijun,Chen Dajing,Huang Xiaojun
A novel strategy based on gradient porous hollow fiber membrane (GPF) is proposed for the modular assembly of enzyme-nanozyme cascade systems. The porous structure of GPF provided sufficient specific surface area, while the gradient structure effectively minimized the leaching of enzymes and nanozymes. To enhance stability, we prepared and immobilized metal–organic framework (MOF) nanozymes, resulting in the fabrication of GPF-MOF with excellent stability and reusability for colorimetric H 2 O 2 detection. To improve specificity and expand the detection range, micro-crosslinked natural enzymes were modularly assembled, using glucose oxidase as the model enzyme. The assembled system, GPF-mGOx@MOF, achieved a low detection limit of 0.009 mM and a linear range of 0.2 to 11 mM. The sensor retained 87.2% and 80.7% of initial activity after being stored for 49 days and 9 recycles, respectively. Additionally, the reliability of the biosensor was validated through glucose determination of human blood and urine samples, yielding comparable results to a commercial glucose meter. Graphical Abstract
研究领域:
生物传感器
·
纳米技术
·
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）