INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2023
·Vol. 24
·期 24
·17280
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IF 5.6
Hui Li,Mingchao Wang,Kai Qu,Ruiming Xu,Haibo Zhu
It is widely recognized that macrophage cholesterol efflux mediated by the ATP-binding cassette transporter A1 (ABCA1) constitutes the initial and rate-limiting step of reverse cholesterol transport (RCT), displaying a negative correlation with the development of atherosclerosis. Although the transcriptional regulation of ABCA1 has been extensively studied in previous research, the impact of post-translational regulation on its expression remains to be elucidated. In this study, we report an AMP-activated protein kinase (AMPK) agonist called ((2R,3S,4R,5R)-3,4-dihydroxy-5-(6-((3-hydroxyphenyl) amino)-9H-purin-9-yl) tetrahydrofuran-2-yl) methyl dihydrogen phosphate (MP), which enhances ABCA1 expression through post-translational regulation rather than transcriptional regulation. By integrating the findings of multiple experiments, it is confirmed that MP directly binds to AMPK with a moderate binding affinity, subsequently triggering its allosteric activation. Further investigations conducted on macrophages unveil a novel mechanism through which MP modulates ABCA1 expression. Specifically, MP downregulates the Cav1.2 channel to obstruct the influx of extracellular Ca2+, thereby diminishing intracellular Ca2+levels, suppressing calcium-activated calpain activity, and reducing the interaction strength between calpain and ABCA1. This cascade of events culminates in the deceleration of calpain-mediated degradation of ABCA1. In conclusion, MP emerges as a potentially promising candidate compound for developing agents aimed at enhancing ABCA1 stability and boosting cellular cholesterol efflux and RCT.Keywords:ATP-binding cassette transporter A1;reverse cholesterol transport;AMP-activated protein kinase;MP;calpain-mediated degradation
研究领域:
心血管研究
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药理学
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分子生物学
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
·
IF 5.4
Jufen Yan,Lu Dai,Pei Wang,Yongsheng He,Qi Wang,Yiliang Chen,Haowei Zhang,Abdallah S. Ali,Rasha H. Ahmed,Ivana Radojcic Redovnikovic,Kui Zhang,Hongliang Wang,Xinya Han
The development of multifunctional materials capable of serving both as high-efficiency adsorbents and high-performance supercapacitors is a critical challenge for environmental and energy applications. In this study, a novel zeolitic imidazole framework-8/mycelial pellet (ZIF-8/MP) composite was successfully synthesized using a simple in-situ growth and staged adsorption process. The MP with its three-dimensional porous structure, served as a scaffold, facilitating the uniform distribution of ZIF-8 across the porous carbon matrix. The resulting ZIF-8 in the ZIF-8/MP composite demonstrated a remarkable adsorption capacity for Congo red (CR), with a maximum capacity of 1428.57 mg/g. The adsorption mechanism was attributed to electrostatic interactions, zinc coordination, hydrogen bonding, and π–π conjugation, as confirmed by FTIR, XPS, and DFT analyses. After adsorption, the carbonized ZIF-8/MP composite demonstrated outstanding supercapacitor performance, achieving a specific capacitance of 113.4 F/g at 1 A/g, and maintaining 93 % capacitance retention after 5000 charge/discharge cycles. Additionally, the ZIF-8/MP composite effectively reduced the toxicity of CR, as demonstrated by phytotoxicity assays, underscoring its potential for environmental remediation. This innovative approach provides an eco-friendly, easily separable, efficient, and practical multifunctional material for transforming hazardous waste into valuable resources for environmental detoxification and energy storage applications.
研究领域:
环境科学
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材料科学
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能源科学
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DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2020
·Vol. 55
·期 11
·4701-4716
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IF 3.553
Ma Jianfeng,Zhao Hongxia,Shang Yazhuo,Peng Changjun,Liu Honglai,Zheng Shuzhen,Wang Tao,Qi Shuai,Ran Qianping
Abstract The presence of a highly alkaline environment or large amounts of Ca 2+ in concrete exerts a great influence on the dispersion of graphene oxide (GO) particles. Therefore, the development of a highly efficient GO dispersant to adapt to complex concrete environments is important. In this work, novel phosphate-modified small molecular polydentate polymers were synthesized and used as efficient dispersants for cement and high-concentration GO solutions. The chemical structure of the polymers was confirmed by 1 H NMR and IR spectroscopy, and the effect of adsorption groups on the adsorption ability of polymers was studied by using adsorption experiments and theoretical calculations. Results showed that the dispersant inositol hexaphosphate–methoxy polyether (IHP-MP) features a high adsorption equilibrium constant and strong adsorption ability. IHP-MP exhibited not only good water-reducing and slump-retaining properties but also remarkable cement hydration-retarding effects due to its numerous phosphate groups. IHP-MP could be used as an auxiliary GO dispersant with the comb polymer PCE to effectively reduce the viscosity and improve the stability of GO solutions. SEM, TGA and IR characterization indicated that IHP-MP strongly interacts with the surface groups of GO particles. Graphic Abstract
研究领域:
材料科学
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化学工程
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聚合物化学
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土木工程
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DOI
高影响因子
LAB ON A CHIP
·
2024
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IF 6.1
Yushen Wang,Junlei Han,Wenteng Tang,Xiaolong Zhang,Jiemeng Ding,Zhipeng Xu,Wei Song,Xinyu Li,Li Wang
Microplastics (MPs) are pervasive pollutants present in various environments. They have the capability to infiltrate the human gastrointestinal tract through avenues like water and food, and ultimately accumulating within the liver. However, due to the absence of reliable platforms, the transportation, uptake, and damage of microplastics in the gut-liver axis remain unclear. Here, we present the development of a gut-liver-on-a-chip (GLOC) featuring biomimetic intestinal peristalsis and a dynamic hepatic flow environment, exploring the translocation in the intestines and accumulation in the liver of MPs following oral ingestion. In comparison to conventional co-culture platforms, this chip has the capability to mimic essential physical microenvironments found within the intestines and liver (e.g., intestinal peristalsis and liver blood flow). It effectively reproduces the physiological characteristics of the intestine and liver (e.g., intestinal barrier and liver metabolism). Moreover, we infused polyethylene MPs with a diameter of 100 nm into the intestinal and hepatic chambers (concentrations ranging from 0 to 1 mg mL−1). We observed that as intestinal peristalsis increased (0%, 1%, 3%, 5%), the transport rate of MPs decreased, while the levels of oxidative stress and damage in hepatic cells decreased correspondingly. Our GLOC elucidates the process of MP transport in the intestine and uptake in the liver following oral ingestion. It underscores the critical role of intestinal peristalsis in protecting the liver from damage, and provides a novel research platform for assessing the organ-specific effects of MPs.
CAS / MDL:
研究领域:
环境科学
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生物医学工程
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毒理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY
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2023
·
IF 2.2
Yuanping Zhang,Boyu Ma,Xinlei Jia
Melamine polyphosphate (MP), a halogen-free flame retardant was utilized to prepare flame retardant enhanced montmorillonite (MP-MMT), which was introduced into polymethyl methacrylate (PMMA) by emulsion polymerization to improve its heat resistance and flame retardancy. The decorated PMMA were characterized by SEM, FTIR, XRD, and XPS, indicating that MP-MMT/PMMA composites were successfully prepared. The resulting composites exhibited excellent heat resistance, the temperature at maximum weight loss rate was increased from 360 °C to 377 °C, and the char residue at 500 °C was increased to 1.93%. In addition, the LOI value of PMMA composite system was increased from 17.2% to 22.4%, and MP-MMT/PMMA-3 achieved UL-94 V-1 rating. Compared with pure PMMA, the total heat release and peak heat release rate of MP-MMT/PMMA were reduced by 23.0% and 32.4%, respectively. The total smoke release of the optimal flame-retardant PMMA resin decreased by 22.1%, the CO production increased slightly, and the CO2 production decreased significantly. This indicates that the incorporation of MP-MMT has a good effect on flame retardancy. Finally, the flame retardant mechanism of MP-MMT to inhibit PMMA combustion was analyzed. The intercalation modification of MMT is potentially valuable for flame retardant studies of PMMA.
研究领域:
材料科学
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高分子化学
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产品关联
DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
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2022
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IF 4.446
Jiacong Wu,Yi Wang,Juan Han,Lei Wang,Chunmei Li,Yanli Mao,Yun Wang
In this work, a mesoporous Zr-based MOF (MP-UIO-66) was first synthesized by biomineralization method using dextran as template and the resultant particles were applied in the immobilization of cellulase . Compared with conventional UIO-66, MP-UIO-66 has more mesoporous channels (4.6 nm) that allow cellulase (3.4 * 12.4 nm) to be entrapped into the channels, improving loading capacity and stability of immobilized enzyme . Specifically, the results showed that MP-UIO-66 had an excellent loading capacity (265 mg g −1 ) for cellulase, and the residual enzyme activity of PVP-cellulase@MP-UIO-66 (PCMU) was still about 83% after 6 cycles. Moreover, the suitable pore structure exposes the active center of cellulase to the external environment, which facilitates the mass transfer of the substrate. Based on Michaelis-Menten constant analysis, the substrate affinity (13.355 mg mL −1 ) and catalytic efficiency (1.311 mL mg −1 min −1 ) of PCMU were better than those of free cellulase (14.525 mg mL −1 , 0.732 mL mg −1 min −1 ).
研究领域:
生物化学
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材料科学
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产品关联
DOI
高影响因子
MEDICAL PHYSICS
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2024
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IF 3.2
Shiman Wu,Chaochao Wang,Junjie Xia,Dijia Sun,Pu-Yeh Wu,Yue Wu,Zhenwei Yao,Yan Ren,Qi Zhang
Background The relaxivity of magnetic resonance imaging (MRI) contrast agents is primarily attributed to metal ions such as gadolinium (Gd) and iron. However, the impact of organic solutes on relaxivity, particularly through alterations in water molecule dynamics, has not been thoroughly investigated. This research was aimed to explore how organic solutes affect the relaxivities of water and Gd-based contrast agents (GBCAs), potentially revealing new aspects for the development of contrast agents. Purpose To investigate the effects of different proportions of water-soluble organic solvents mixed with pure water and GBCA on T 1 and T 2 relaxivities. Methods Ethanol, dimethyl sulfoxide (DMSO), 1,4-dioxane, glycerin, ethylene glycol, diethylene glycol, polyethylene glycol (PEG) 200, and PEG 400 were mixed with ultrapure water in various ratios (100:0, 90:10, 80:20, 70:30, 60:40, 50:50, 40:60, 30:70, 20:80, 10:90, 0:100). GBCA was added to these mixtures at a concentration of 0.05 mmol/L. The mixtures underwent T 1 and T 2 mapping scans using a 3.0 Tesla MRI machine, and the relaxivities R 1 (1/T 1 ) and R 2 (1/T 2 ) were calculated and compared. Results The relaxivity R 1 of ethanol, 1,4-dioxane, and DMSO mixtures with water or GBCA, as well as R 2 of DMSO mixtures with GBCA, initially increased and then decreased. Conversely, R 1 and R 2 increased significantly with higher proportions of diethylene glycol, PEG 200, and glycerin in the mixtures with GBCA. The increase in relaxivity R 1 was correlated with greater viscosity. When the proportion of DMSO and diethylene glycol exceeded 20%, minimal variability in R 2 was observed as the water content decreased. Conclusions Adding organic solvents to water and paramagnetic relaxation reagents could alter T 1 and T 2 relaxivities, suggesting potential new directions for modifying current contrast agents. Additionally, increasing the viscosity of the contrast agent was found to enhance the relaxivity.
研究领域:
医学影像学
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生物物理学
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产品关联
DOI
PubMed
高影响因子
MEDICAL PHYSICS
·
2019
·Vol. 46
·期 12
·5467-5477
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IF 3.177
Diya Wang,Yuchao Sang,Xinyu Zhang,Hong Hu,Shukuan Lu,Yu Zhang,Chaoying Fu,Guy Cloutier,Mingxi Wan
Purpose The Nakagami statistical model and Nakagami shape parameter m have been widely used in linear tissue characterization and preliminarily characterized the envelope distributions of nonlinear encapsulated microbubbles (EMBs). However, the Nakagami distribution of nonlinear scattering EMBs lacked a systematical investigation. Thus, this study aimed to investigate the Nakagami distribution of EMBs and illustrate the impact of EMBs' nonlinearity on the Nakagami model. Method A group of simulated EMB phantoms and in vitro EMB dilutions with an increasing concentration distribution under various EMB nonlinearities, as regulated by acoustic parameters, were characterized by using the window-modulated compounding Greenwood–Durand estimator. Results Raw envelope histograms of simulated and in vitro EMBs were well matched with the Nakagami distribution with a high correlation coefficient of 0.965 ± 0.021 ( P < 0.005). The mean values and gradients of m parameters of simulated and in vitro EMBs were smaller than those of linear scatterers due to the stronger nonlinearity. These m values exhibited a quasi-linear improvement with the increase in second harmonic nonlinear-to-linear component ratio regulated by pulse lengths and excitation frequencies at low- and high-concentration conditions. Conclusions The Nakagami distribution was suitable for the EMBs characterization but the corresponding m parameter was affected by the EMBs' nonlinearity. These validations provided support and nonlinear impact assessment for the EMBs' characterization using the Nakagami statistical model in the future.
研究领域:
超声学
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生物医学工程
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DOI
PubMed
高影响因子
MEDICAL PHYSICS
·
2023
·Vol. 50
·期 10
·6624-6636
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IF 3.8
Jin Dong Cho,Hyeongmin Jin,Seongmoon Jung,Jaeman Son,Chang Heon Choi,Jong Min Park,Jin Sung Kim,Jung-in Kim
Background Patient-specific QA verification ensures patient safety and treatment by verifying radiation delivery and dose calculations in treatment plans for errors. However, a two-dimensional (2D) dose distribution is insufficient for detecting information on the three-dimensional (3D) dose delivered to the patient. In addition, 3D radiochromic plastic dosimeters (RPDs) such as PRESAGE ® represent the volume effect in which the dosimeters have different sensitivities according to the size of the dosimeters. Therefore, to solve the volume effect, a Quasi-3D dosimetry system was proposed to perform patient-specific QA using predetermined-sized and multiple RPDs. Purpose For patient-specific quality assurance (QA) in radiation treatment, this study aims to assess a quasi-3D dosimetry system using an RPD. Methods Gamma analysis was performed to verify the agreement between the measured and estimated dose distributions of intensity-modulated radiotherapy (IMRT) and volumetric modulated arc therapy (VMAT). We fabricated cylindrical RPDs and a quasi-3D dosimetry phantom. A practicability test for a pancreatic patient utilized a quasi-3D dosimetry device, an in-house RPD, and a quasi-3D phantom. The dose distribution of the VMAT design dictated the placement of nine RPDs. Moreover, a 2D diode array detector was used for 2D gamma analysis (MapCHECK2). The patient-specific QA was performed for IMRT, VMAT, and stereotactic ablative radiotherapy (SABR) in 20 prostate and head-and-neck patients. For each patient, six RPDs were positioned according to the dose distribution. VMAT SABR and IMRT/VMAT plans employed a 2%/2 mm gamma criterion, whereas IMRT/VMAT plans used a 3%/2 mm gamma criterion, a 10% threshold value, and a 90% passing rate tolerance. 3D gamma analysis was conducted using the 3D Slicer software. Results The average gamma passing rates with 2%/2 mm and 3%/3 mm criteria for relative dose distribution were 91.6% ± 1.4% and 99.4% ± 0.7% for the 3D gamma analysis using the quasi-3D dosimetry system, respectively, and 97.5% and 99.3% for 2D gamma analysis using MapCHECK2, respectively. The 3D gamma analysis for patient-specific QA of 20 patients showed passing rates of over 90% with 2%/2 mm, 3%/2 mm, and 3%/3 mm criteria. Conclusions The quasi-3D dosimetry system was evaluated by performing patient-specific QAs with RPDs and quasi-3D phantom. The gamma indices for all RPDs showed more than 90% for 2%/2 mm, 3%/2 mm, and 3%/3 mm criteria. We verified the feasibility of a quasi-3D dosimetry system by performing the conventional patient-specific QA with the quasi-3D dosimeters.
研究领域:
医学物理
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放射肿瘤学
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产品关联
DOI
PubMed
高影响因子
Natural Product Communications
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2020
·
IF 0.468
Bo Tao,Zong-ning Yin
Thiolated hyaluronic acid was synthesized, and a quantification method was established to determine the content of the thiol group. Three thiol compounds, 2-nitro-5-mercapto-benzoic acid (TNB), 2- mercaptonicotinic acid (2-MNA), and mercaptopyridine (MPD), were tested for their efficiency in forming adducts with hyaluronic acid (HA) through disulfide bonds. Our results showed that MPD exhibited the highest disulfide exchange efficiency when the reaction was conducted at the optimal pH value. The synthesized hyaluronic acid-SS-mercaptopurine (HA-SS-MP) was characterized by 1H-nuclear magnetic resonance spectroscopy. The HA-SS-MP micelle showed a particle size of 264.4 nm in aqueous solution. An in vitro release experiment demonstrated that the releasing rate of 6-MP from HA-SS-MP in the release medium containing glutathione (GSH) was much higher than that in the release medium without GSH. Cell uptake experiments demonstrated the CD44 targeting of hyaluronic acid. Cytotoxicity tests in mouse melanoma cells (B16F10) showed that HA-SS-MP can kill melanoma cells effectively and with lower cytotoxicity, compared with the active ingredient, 6-MP.
研究领域:
药学
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肿瘤学
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生物化学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yuan Li,Limin Wang,Lu Xue,Qianfei Jia,Shufen Li,Yuxi Qin,Zijian Wu
Recently, research on the plant-based oleogels, has gained significant attention as a promising approach for oil formulation. In this research, emulsion-templated technique utilizing millet prolamin (MP) as oleogelator was employed for crafting edible oleogels from various vegetable oils of varying degree of unsaturation. The focus of the study was to examine the formation process, structural characteristics, rheological properties, and lipid digestion of these oleogels. The results indicated that MP-based oleogels exhibited uniform morphology and low oil loss. The construction of the oleogel network could be attributed to hydrogen bonding between oleogelator molecules, hydrophobic interactions among the oleogelator molecules and between the oleogelator and oil, as well as van der Waals forces between the oil molecules themselves. Increasing MP concentration formed a denser oleogel network, and exhibited greater elastic moduli (G′) and viscous moduli (G′′). Additionally, 8 % MP oleogel prepared from flaxseed oil with high linolenic acid showed low oil loss, high hardness and efficient release of free fatty acids. These results indicated the potential of MP as an oleogelator in creating oleogels, and it is promising for fabricating trans-free and low-saturated oleogel-based products.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
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2025
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IF 4.7
Tao Huang,Pengju Xu,Dinghan Wang,Wanxin Li,Ruohan Wei,Qian Ji,Hong Lin,Jiefeng Shen
An organic–inorganic hybrid microsphere with excellent thermal expansion properties was successfully synthesized and investigated. This study systematically examined the effects of various types and concentrations of silica-modified acrylic monomers on the thermal expansion characteristics of the microspheres. Furthermore, to enhance the high-temperature durability of thermal expansion microspheres (TEMs), an acrylate-modified polyhedral oligomeric silsesquioxane (MP-POSS) with high compatibility was synthesized as a cross-linker, and its synergistic effect on the thermal properties of TEMs was thoroughly analyzed. The core/shell polymers were characterized in terms of their chemical structures, thermal expansion behaviors, and morphology. Experimental results indicated that when triisopropylsilyl methacrylate (TISMA) was incorporated at 20 wt % and the bifunctional acrylate ester of MP-POSS was utilized as the cross-linker, the optimized TEMs exhibited a maximum expansion temperature of 230 °C, which was 23 °C higher than that of the conventional acrylate-based TEMs. Additionally, the foamed microspheres maintained their structure at 190 °C for 39 min without significant collapse. It was believed that the hybrid microspheres would provide a potential application value to fabricate lightweight materials that require high-temperature treatment, such as ceramics, plastics, rubber, and other industrial products.
研究领域:
材料科学
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高分子化学
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纳米技术
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热分析
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）