ACS Catalysis
·
2022
·
IF 13.7
Mingwei Zhang,Linlin Liu,Li Wang,Xiangwen Zhang,Guozhu Li
Hydrocracking of long-chain paraffins is a key process in petroleum refining. A fundamental understanding of the reaction on the bifunctional catalyst is of great importance for controllable cracking. In this work, a mechanism study on the hydrocracking of long-chain paraffins catalyzed by Pt/ZSM-23 has been carried out. The distribution and accessibility of acid sites on MTT channels are purposefully adjusted. Based on reasonable simplification, the reaction data are analyzed to reveal the shape selectivity, reaction path, and catalytic mechanism. The applicability of the pore-mouth mechanism in this system has been carefully verified by experiments. Both protonated cyclopropane (PCP) and β-scission paths occurred simultaneously at the pore mouths, accounting for 60 and 40%, respectively. The proportions of PCP and β-scission paths with different chain lengths inserted in the pore are quantitatively calculated, which are multimodal. Shape selectivity of the teardrop-shaped MTT skeletal structure is investigated by the joint experimental–theoretical method. The insertion of the paraffin chain in the MTT channel is discretely segmented by integral multiples of four sequential carbon atoms starting at one end of the chain, which is followed by cracking through the mixed PCP and β-scission paths at pore mouths.
研究领域:
石油化学
·
催化反应工程
✓ 已验证
DOI
高影响因子
FUEL PROCESSING TECHNOLOGY
·
2022
·
IF 8.129
Linlin Liu,Mingwei Zhang,Li Wang,Xiangwen Zhang,Guozhu Li
Bifunctional catalysts with high isomerization activity and mono-branched isomer selectivity are urgently demanded for dewaxing long-chain n -alkanes. In particular, branching location of mono-branched isomer is difficult to control by current techniques. In this study, SSZ-32 zeolite with tunable distribution depth of acid sites in micropore channels is successfully prepared by ion exchange under partial template protection. Loaded with similar Pt nanoparticles , these SSZ-32 catalysts exhibit tunable activity and selectivity for hydroisomerization of n- hexadecane . Characterization results show that the adsorption model and insertion depth of n -alkane is altered by changing the distribution depth of acid sites in SSZ-32 micropore channels. When acid sites distribute in a moderate depth (∼ 4.5 nm) of the micropore channels, high yield of center-position mono-branched isomers (46.85%) is achieved, cracking reaction is significantly inhibited. The mole ratio of 6−/7−/8-methyl isomers to 2−/3-methyl isomers was increased to at least 1.3 times of the traditional catalyst with acid sites distributed throughout the micropores. The cold flow properties of lube base oils in isomerized dewaxing of Fischer-Tropsch wax are improved significantly by achieving an optimal and effective diffusion depth of reaction intermediates in the MTT micropore channels. Efficient and selective hydroisomerization of long-chain n -alkane is realized on Pt/SSZ-32 prepared by the facile partial-detemplation method.
研究领域:
催化化学
·
材料科学
·
石油炼制工程
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Jing-Yu Liu,Xiao-Xiao Hou,Zhuo-Yu Li,Shu-Hua Shan,Ming-Chang Chang,Cui-Ping Feng,Yin Wei
The fruiting body of Hericium erinaceus has been used to treat digestive system disorder-related diseases for over 2000 years in China. A novel polysaccharide, HEFP-2b, was obtained from H. erinaceus fruiting bodies. Physical and chemical analysis showed that HEFP-2b consisted of fucose, galactose, glucose, and mannose in molar ratio of 11.81:22.82:44.28:21.09, and that its molecular weight was 3.252 × 10 4 Da. The backbone of HEFP-2b consisted of →6)-linked-α-D-Glc p -(1→ and →4)-β-D-Gal p -(1→ and →3,6) -α-D-Man p linkage, with two side-branching units of (1→ and →6)-β-D-Gal p and (1→ and →4)-α-D-Man p , terminated by Glc and Fuc. The results of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cell cycle arrest experiments revealed that HEFP-2b considerably inhibited the growth of colon cancer cells (HCT-116) in vitro . The growth inhibitory effects of HEFP-2b correlated with their ability to arrest the cell cycle at the S -phase. Our results will provide valuable information for future studies on HEFP-2b as a novel health-promoting functional food ingredient that can be used for treating colon cancer.
研究领域:
药理学
·
肿瘤学
·
生物化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Phytochemistry Letters
·
2015
·
IF 1.45
Zhen-Jie Lia,Chun-Ping Wan,Le Cai,Shu-Quan Li,Xi Zheng,Yan Qi,Jian-Wei Dong,Tian-Peng Yin,Zhi-Xian Zhou,Ning-Hua Tan,Zhong-Tao Ding
Twenty terpenoids, including a new triterpenoid ( 1 ) and a new monoterpenoid ( 20 ), were isolated from the branches and leaves of Pyrus pashia . The structures of two new compounds were determined to be 2α, 3β, 27-trihydroxyolean-12-en-28-oic acid ( 1 ) and (4α)-3-(5,5-dimethyltetrahydrofuranyl)-1-buten-3-ol 3- O -β- d -glucopyranoside ( 20 ) on the basis of spectroscopic analysis (IR, HRESIMS, 1D and 2D NMR) and chemical method. Some of the isolated compounds were evaluated for their cytotoxic activity against a panel of human cancer cell lines by MTT assay, using cisplatin as a positive control. Compound 14 exhibited cytotoxic activities against A549 (IC 50 = 19.18 ± 4.26 μM), Hela (IC 50 = 12.56 ± 3.89 μM), SGC7901 (IC 50 = 10.48 ± 1.95 μM) and NHI-1975 (IC 50 = 7.38 ± 2.31 μM) cell lines as well as compound 12 displayed cytotoxic activities against A549 (IC 50 = 14.71 ± 1.47 μM) and Hela (IC 50 = 12.22 ± 1.88 μM) cell lines.
研究领域:
植物化学
·
药理学
·
肿瘤学
✓ 已验证
产品关联
DOI
高影响因子
ARCHIVES OF PHARMACAL RESEARCH
·
2016
·Vol. 39
·期 7
·871-877
·
IF 2.49
Li Da-hong,Guo Jia,Bin Wen,Zhao Nan,Wang Kai-bo,Li Jian-yong,Li Zhan-lin,Hua Hui-ming
Two novel rare chloro-containing benzylisoquinoline alkaloids, thalfoliolosumines A ( 1 ) and B ( 2 ), along with eight known isoquinoline alkaloids ( 3 – 10 ) were isolated from the whole plant of Thalictrum foliolosum . The structures of these compounds were elucidated by spectral analyses, including 1D and 2D NMR (COSY, HSQC, HMBC and NOESY) experiments. The antiproliferative effects of all the isolated compounds were evaluated by MTT method against MCF-7, PC-3, and U937 cells, and trypan blue method against HL-60 cells. New compounds 1 and 2 exhibited moderate in vitro antiproliferative activity against MCF-7, PC-3, and HL-60 cells, and good inhibitory effects against U937 cells with IC 50 values of 7.50 and 6.97 μM, respectively. Compounds 7 and 10 showed the strongest in vitro antiproliferative with IC 50 values of 0.93 and 1.69 μM against HL-60 cell line. The antioxidant properties were also measured, bisbenzyltetrahydroisoquinoline alkaloids 3 – 6 showed the strongest antioxidant activities in ABTS assay.
研究领域:
药理学
·
植物化学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MEDICINAL CHEMISTRY RESEARCH
·
2021
·Vol. 30
·期 10
·1856-1867
·
IF 1.965
Yin Yu-dong,Liu Jing-jing,Liao Wen-fei,Guo Xian-kun,Zhang Li-shan,Mo Wei-bin,Cheng Ke-guang
As 17 β -estradiol and its derivatives have good affinity with estrogen receptors and have unique antitumor properties, O 1 -propargyl- N 4 , N 4 -bis(2-chloroethyl)-4-aminophenol and N 4 , N 4 -dipropargyl- N 1 , N 1 -bis(2-chloroethyl)-1,4-phenylenediamine were respectively combined with 17 β -estradiol through linkers of different lengths. These targeted novel nitrogen mustard 17 β -estradiol derivatives were tested antiproliferative activity using the MTT assay. The results illustrated that these compounds were less toxicity to HL-7702 (human liver cells) and SKOV-3, though all their activity potency was moderate or weak aganist the MDA-MB-231, MCF-7 and BEL-7404 cell lines. The inhibitory activity of the O 1 -propargyl- N 4 , N 4 -bis (2-chloroethyl)-4-aminophenol derivatives (compounds III-1~6 and VII-1~4 ) were obviously better than the N 4 -dipropargyl- N 1 , N 1 -bis (2-chloroethyl) -1,4-phenylenediamine derivatives (compounds IV-1~6 and VIII-1~4 ) against tested tumor cell lines. And three hybrids (compounds III-2 , III-6 , and VII-3 ) exhibited good inhibitory activity against BEL-7404 cell line (IC 50 = 13.5, 15.3 and 13.9 μM, respectively). After that, we performed KEGG pathway analysis on compound III-6 , and the results showed that III-6 mainly induced breast cancer cell apoptosis by regulating the expression of the proteins in NF-κ B pathway.
研究领域:
药理学
·
肿瘤学
·
分子生物学
✓ 已验证
DOI
高影响因子
CHEMISTRY & BIODIVERSITY
·
2018
·Vol. 15
·期 3
·e1700513
·
IF 1.617
Xiaoping Zhan,Yun Xu,Qi Qi,Yaolin Wang,Huiying Shi,Zhenmin Mao
A series of novel quinazolinone derivatives containing a substituted amino moiety were synthesized, evaluated for their cytotoxic and antibacterial activities. The results of MTT assay showed that all synthesized target compounds 5A – 5O showed potent cytotoxicity against SGC-7901 ( IC 50 , 0.72 – 1.41 μ m ). Moreover, the compounds 5D , 5I , and 5K showed better selectivity as compared with positive controls pemetrexed and MTX due to weak cytotoxicity against normal tissue cell line HUVSMC. Among synthesized compounds, the compounds 5E , 5J , 5L , and 5N showed broad-spectrum cytotoxic activities against at least four cancer cell lines at a micromolar level. The results of antibacteria evaluation revealed that all synthesized compounds showed good to moderate antibacterial activities against Gram -negative bacteria Escherichia coli . Among them, the MIC values of the compounds 5C , 5F , and 5M were 0.31 μg/mL. Graphical
研究领域:
药物化学
·
肿瘤学
·
微生物学
✓ 已验证
DOI
高影响因子
Inorganics
·
2022
·Vol. 10
·期 11
·202
·
IF 3.149
Liang Qin,Fenglan Liang,Yan Li,Jiana Wu,Shiyuan Guan,Meiyin Wu,Shiling Xie,Manshi Luo,Deyun Ma
A hydrostable 2D Zn-based MOF, {[Zn(5-PIA)(imbm)]·2H2O}n(1) (5-H2PIA = 5-propoxy-isophthalic acid, imbm = 1,4-di(1H-imidazol-1-yl)benzene), was synthesized and structurally characterized. Complex1shows good water and thermal stability based on the TGA and PXRD analyses and displays a 2D framework with 1D channels of 4.8 × 13.8 and 10.0 × 8.3 Å2along theaaxis. The 5-fluorouracil (5-FU) payload in activated complex1(complex1a) is 19.3 wt%, and the cumulative release value of 5-FU at 120 h was about 70.04% in PBS (pH 7.4) at 310 K. In vitro MTT assays did not reveal any cytotoxic effect of NIH-3T3 and HEK-293 cells when the concentration of1was below 500 μg/mL and 5 μg/mL, respectively. No morphological abnormalities were observed on zebrafish exposed to complex1.Keywords:zinc-organic framework;drug delivery;5-fluorouracil;MTT assays;zebrafish toxicity
研究领域:
材料科学与工程
·
医学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
PLoS One
·
2017
·Vol. 12
·期 8
·e0183941
·
IF 2.806
Wen Che,Yun Bao,Feng Tang
Background
Resistance to human epidermal growth factor receptor-2 (HER2)-targeted therapies is common, and results in treatment failure and new tumor progression. C35 is over-expressed in many invasive breast cancer endogenously, and functions as an oncogene in breast cancer cell lines.
Methods
The current study aims to investigate effects of C35 on cell viability and migration of HER2-positive breast tumor cells and explore possible mechanisms. The protein expression of C35 in BT-474 breast ductal carcinoma cells was interfered by siRNAs. MTT assay was utilized to detect the viability of BT474 breast ductal carcinoma cells after transfection. The migration of BT474 breast ductal carcinoma cells was examined by cell wound scratch assay.
Results
In addition, the protein expression of C35, HER2, AKT and p-AKT in breast ductal carcinoma cells were detected by Western Blot. The protein expression of C35 was weaker in siRNA1 and siRNA2 groups compared with the control group. The relative expression ratio of C35 to β-actin decreased significantly in siRNA2 and siRNA1 groups. When C35 expression was interfered by siRNA1 and siRNA2 for 48 h, the viability of BT474 breast ductal carcinoma cells decreased compared with the control group. In addition, the migration of breast ductal carcinoma cells decreased when C35 expression was interfered by siRNA1 for 24 h, and interfered by siRNA1 and siRNA2 for 48 h.
研究领域:
肿瘤学
·
分子生物学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2013
·Vol. 18
·期 9
·11496-11511
·
IF 2.428
Rui Liu,Hongchi Zhang,Maosen Yuan,Jiao Zhou,Qin Tu,Jian-Jun Liu,Jinyi Wang
Two series of apigenin [5,7-dihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one] derivatives,3a–3jand4a–4j, were synthesized. The apigenin and alkyl amines moieties of these compounds were separated by C2or C3spacers, respectively. The chemical structures of the apigenin derivatives were confirmed using1H-NMR,13C-NMR, and electrospray ionization mass spectroscopy. Thein vitroantibacterial and antiproliferative activities of all synthesized compounds were determined. Among the tested compounds,4a–4jdisplayed significant antibacterial activity against the tested strains (Staphylococcus aureus,Bacillus subtilis,Escherichia coli, andPseudomonas aeruginosa). Additionally,4ishowed the best inhibitory activity with minimum inhibitory concentrations of 1.95, 3.91, 3.91, and 3.91 μg/mL againstS. aureus,B. subtilis,E. coli, andP. aeruginosa, respectively. The antiproliferative activity of the apigenin derivatives was evaluated by an MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay. We determined that4a–4jdisplayed better growth inhibition activity against four human cancer cell lines, namely, human lung (A549), human cervical (HeLa), human hepatocellular liver (HepG2), and human breast (MCF-7) cancer cells, than the parent apigenin. Compound4jwas found to be the most active antiproliferative compound against the selected cancer cells. Structure-activity relationships were also discussed based on the obtained experimental data.Keywords:synthesis;biological evaluation;apigenin derivatives;antibacterial activity;antiproliferative activity;MTT assay
研究领域:
药理学
·
化学
·
微生物学
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
Cancer Biomarkers
·
2019
·Vol. 24
·期 4
·469-475
·
IF 2.859
Qin Yujing,Li Wenxue,Long Yingli,Zhan Zhijia
OBJECTIVE: This study aims to determine the correlation between p-cofilin expression and cisplatin resistance in patients with ovarian cancer, and also to investigate the role of p-cofilin in prognosis. PATIENTS AND METHODS: The ovarian cancer cell line A2780/DDP resistant to cisplatin was prepared. The cell resistance to cisplatin was measured via MTT assay. The cell invasion capacity was identified via Transwell assay. The mRNA expression and protein level was evaluated via semi-quantitative PCR and Western blot, respectively. The tumor tissues of patients with cisplatin-resistant ovarian cancer were collected. The relationship between prognosis and p-cofilin expression was analyzed. RESULTS: The growth rate of A2780 was similar to that of A2780/DDP. The sensitivity of A2780 to cisplatin was significantly higher than that of A2780/DDP (p< 0.01). The migration capacity of A2780/DDP was significantly increased compared to that of A2780 (p< 0.01), indicating that the cisplatin-resistant cell lines were successfully constructed. Both CFL1 mRNA level and p-cofilin level in A2780/DDP was significantly higher than that in A2780 (p< 0.01). The p-cofilin level in cancer tissues in patients with cisplatin-resistant ovarian cancer was significantly higher than that in patients with cisplatin-sensitive ovarian cancer (p< 0.01). The cisplatin resistance was positively correlated with the p-cofilin expression level (r= 0.802, p= 0.023). The survival time of patients with normal or low level of p-cofilin was significantly longer than that of patients with high expression. CONCLUSION: The cisplatin resistance of ovarian cancer is closely related to the expression level of p-cofilin, which affects the prognosis of patients with ovarian cancer.
研究领域:
肿瘤学
·
分子生物学
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2019
·
IF 3.743
Hao Guo,Xinhuan Wan,Fengju Niu,Jujie Sun,Chenxiao Shi,Jessica Meng Ye,Changzheng Zhou
In this study, we aimed to evaluate the antiviral effect of total flavonoids extracted from Robinia pseudoacacia cv. idaho (RPTF) in vivo and its toxicity on rats with oral gavage. RPTF was prepared by percolation with 70% ethanol for 24 h and its antiviral effect on different kinds of viruses was evaluated in vitro by MTT staining. The long-term toxicity of RPTF on rats was evaluated through the detection of general behavior, body weight, food intake and related organ tissue sections of experimental animals. We found that RPTF produced significantly inhibitory effects on HSV-1 and EV-71 viruses with the therapeutic index TI values 113.8 and 46.2, respectively. Moreover, toxicity evaluation in vivo showed no significantly adverse effects in rats, indicating that RPTF was safe in use. In conclusion, we demonstrated that RPTF, natural compounds in the Chinese traditional medicine, could act as promising and effective antiviral therapeutics with relative safety in use.
研究领域:
药理学
·
病毒学
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）