Analytical Methods
·
2024
·
IF 2.7
Weiqiang Li,Xiaoxue Liu,Pianpian He,Wentao Hu,Kaijie Tang,Yangping Wen,Qian Zeng,Huiyi Tang,Yaxuan Lei,Xin Liu
Deoxynivalenol (DON) has drawn considerable attention for its obvious pathogenicity and wide use in agro-products, which cause a potential threat to human health. In this work, an electrochemical immunosensor is developed for the highly sensitive and selective detection of DON in wheat flour using AuNPs-BP-MWCNTs-COOH and antibodies. The AuNPs-BP-MWCNTs-COOH nanocomposite was prepared via an in situ reduction reaction and ultrasonic-assisted liquid-phase exfoliation. The nanocomposite exhibits a larger surface area, decent stability, excellent electron transfer capability, good protein binding capability and prominent specificity. The plentiful carboxyl group on the nanocomposite can bind to the amino group of the antibody, and AuNPs have an affinity for the sulfhydryl group of the antibody, which makes it feasible for the nanocomposite to load the antibody. The peak currents are plotted against the logarithm of DON concentration from 0.002 to 80 ng mL−1 with a limit of detection (LOD) of 0.5 pg mL−1. This approach establishes an effective label-free immunosensor platform for the detection of DON with high sensitivity and selectivity in various food and agricultural products.
研究领域:
食品安全
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分析化学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Haotian Wu,Gang Zhang,Xiaozhan Yang
An electrochemical biosensor based on iron tetroxide (Fe 3 O 4 )/carboxylated carbon nanotubes (MWCNTs-COOH)/gold nanoparticles (AuNPs) was designed for the detection of alpha-fetoprotein (AFP), which is often used as an important indicator for the early clinical diagnosis of liver cancer markers. The Fe 3 O 4 /MWCNTs-COOH nanocomposite was synthesized by a solvothermal method and it combined with gold nanoparticles (AuNPs) deposited at the constant potential on a glassy carbon electrode to form Fe 3 O 4 /MWCNTs-COOH/AuNPs, which intensifies the electrical signal while the large active sites enable more stable immobilization of the AFP monoclonal antibodies on the electrode. The electrochemical performance of Fe 3 O 4 /MWCNTs-COOH/AuNPs was investigated in detail and the electrochemical response signal after the immune reaction with the AFP antigen-antibody was recorded. The peak current Ip of the response signal is linearly proportional to the lg c AFP in the range of 1 pg mL −1 –10 μg mL −1 , with a detection limit of 1.09034 pg mL −1 and good performance in clinical sample testing. The proposed sensor has shown great application and development potential in clinical medicine field.
研究领域:
生物化学
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纳米技术
·
生物医学工程
·
临床医学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Nana Tang,Shuting Shi,Chuanqin Zhou,Jianhua Ding,Aiting Chen,Quanguo He,Wu Wang
The misuse of the antibiotic ampicillin (AMP) has led to the presence of antibiotic residues in related products, which enter the human body through the ecological or food chain to produce a variety of toxic side effects. There is an urgent need to develop enzyme-based assays with higher specificity to determine antibiotic residues in food. Hereby, bismuth tungstate (Bi 2 WO 6 ) was synthesized using a simple hydrothermal and calcination process. Carboxylated multi-walled carbon nanotubes (MWCNTs-COOH) were mixed with Bi 2 WO 6 by ultrasonication to form composite as modifier on glassy carbon electrode (GCE) surface. Meanwhile, metal β-lactamases (MBLs) were mixed with chitosan to be immobilized on it to obtain the MBLs/CS/Bi 2 WO 6 /MWCNTs-COOH/GCE. Under the optimal conditions, differential pulse voltammetry (DPV) was used for the quantitative detection of AMP. The experimental results showed that the peak currents were linearly correlated with the concentrations of AMP (0.0007–0.05 μM) and (0.1–10 µM) (R 2 = 0.9965 and 0.9956), and the detection limit was 0.17 nM (S/N = 3). The method has good repeatability, reproducibility, stability and immunity to interference, and can be applied to the highly sensitive detection of AMP in real samples with recoveries of 92.4–96.8 % and 94.3–103.0 %.
研究领域:
生物传感
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电化学分析
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食品检测
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产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2022
·
IF 4.598
Wei Zhong,Jin Zou,Yanyu Xie,Jing Yang,Mingfang Li,Shuwu Liu,Yansha Gao,Xiaoqiang Wang,Limin Lu
Herein, a highly sensitive and selective electrochemical sensor for the detection of benomyl (BN), a typical benzimidazole fungicide was proposed based on hierarchical nano-Cu x O decorated MWCNTs-COOH/MXene (nano-Cu x O-MWCNTs-COOH /MXene) composite. The ternary heterostructure presents good electrical conductivity, large specific surface area, and high catalytic activity, exhibiting excellent electrocatalytic activity with enhanced electrochemical signals towards the oxidation of BN. Besides, the embedded nano-Cu x O particles possess strong affinity toward benzimidazole molecules, which could promote the preconcentration of BN, further enhancing the sensitivity, as well as ensuring the high selectivity. Benefitting from the synergistic effect of components in the composite, the developed sensor presents a broad linear range from 10.0 nM to 10.0 μM with a low limit of detection of 3.0 nM (S/N = 3). Meanwhile, the nano-Cu x O-MWCNTs-COOH/MXene electrode also displays good long-term stability and repeatability. Furthermore, this method was successfully applied for the detection of BN in apple samples, illustrating its promising practicability.
研究领域:
分析化学
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环境科学
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2014
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IF 4.086
Zhenping Liu,Haiyun Zhai,Zuanguang Chen,Qing Zhou,Zhixian Liang,Zihao Su
A novel Fe 2 O 3 nano-materials/oxygen functionalized multi-walled carbon nanotubes/triton X-100 modified carbon paste electrode (Fe 2 O 3 /MWCNTs-COOH/OP/CPE) was prepared to simultaneously detect orange G (Or G) and orange II (Or II) by electrochemical methods. Compared with the bare carbon paste electrode (CPE), Fe 2 O 3 /MWCNTs-COOH/OP/CPE exhibited obvious electrocatalytic activities towards Or G and Or II. Furthermore, the two azo dyes were identified and separated successfully, and were simultaneously determined by the redox peaks in cyclic voltammograms (CVs) and differential pulse voltammogram (DPV) under optimum conditions. In DPV, anodic peak current (Ipa) and concentrations were linearly related well for 0.1-20.0 μM Or G and 0.2-50.0 μM Or II. The limits of detection (S/N = 3) of Or G and Or II were 0.05 μM and 0.1 μM, respectively. Therefore, the electrode is suitable for the determination of Or G and Or II in industrial wastewater due to high sensitivity and selectivity, low detection limit, good reproducibility, as well as easy preparation and regeneration.
研究领域:
分析化学
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环境科学
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产品关联
DOI
高影响因子
Biomimetics
·
2022
·Vol. 7
·期 4
·248
·
IF 3.743
Pan Cao,Huming Wang,Mingyi Zhu,Yifeng Fu,Chengqing Yuan
Any surface immersed in sea water will suffer from marine fouling, including underwater sound absorption coatings. Traditional underwater sound absorption coatings rely heavily on the use of toxic, biocide-containing paints to combat biofouling. In this paper, an environmentally-friendly nanocomposite with integrated antifouling and underwater sound absorption properties was fabricated by adopting MWCNTs-COOH and SiO2into PDMS at different ratios. SEM, FTIR and XPS results demonstrated MWCNTs were mixed into PDMS, and the changes in elements were also analyzed. SiO2nanoparticles in PDMS decreased the tensile properties of the coating, while erosion resistance was enhanced. Antibacterial properties of the coatings containing MWCNTs-COOH and SiO2at a ratio of 1:1, 1:3, and 1:5 reached 62.02%, 72.36%, and 74.69%, respectively. In the frequency range of 1500–5000 Hz, the average sound absorption coefficient of PDMS increased from 0.5 to greater than 0.8 after adding MWCNTs-COOH and SiO2, which illustrated that the addition of nanoparticles enhanced the underwater sound absorption performance of the coating. Incorporating MWCNTs-COOH and SiO2nanoparticles into the PDMS matrix to improve its sound absorption and surface antifouling properties provides a promising idea for marine applications.
研究领域:
材料科学
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海洋工程
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环境科学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Zhijuan Wang,Ran Han,Lin Li,Jiaqing Sun,Jingying Yang,Mingfei Pan,Shuo Wang
A facile, ultra-sensitive and cost-effective electrochemical sensor was successfully prepared by combining metal–organic framework ZIF-8 with MWCNTs-COOH, which detecting of tert -butylhydroquinone (TBHQ) in edible oil and fried foods. The hydrothermally synthesized ZIF-8 material with microporous structure was demonstrated to have large specific surface area, and together with the MWCNTs-COOH with high electrical conductivity and catalytic properties, was used as a modified substrate for electrodes. Because of the synergistic effect between ZIF-8 and MWCNTs-COOH, the modified electrode exhibited significant electrocatalytic ability for TBHQ. Under the optimal experimental conditions, the ZIF-8/MWCNTs-COOH modified electrode obtained good linearity when the TBHQ concentration ranged from 0.01 to 25.0 μg mL −1 with the LOD (limit of detection) of 0.009 μg mL −1 (S/N = 3). Moreover, the constructed electrochemical sensor exhibits better anti-interference capability, reproducibility and stability, and can be used to monitor the content of TBHQ in oil and fried foods.
研究领域:
分析化学
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食品科学
·
材料科学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Shan Luo,Yuanyuan Wang,Xianwen Kan
Ratiometric electrochemical sensing provides a new perspective for the accurate and reliable detection of different analytes since inner reference signal can effectively eliminate the internal and external interferences. There is still a challenge to explore new inner references for the development of ratiometric electrochemical sensing. Herein, a kind of redox-active metal-organic frameworks (Cu-THQ MOFs) was synthesized and characterized by scanning electron microscope, transmission electron microscope, X-ray powder diffractometer, and X-ray photoelectron spectroscopy techniques. Cu-THQ MOFs were composited with carboxylic group functionalized multi-walled carbon nantubes (MWCNTs-COOH) to construct a sensitive electrochemical sensor. The modified Cu-THQ MOFs not only showed good catalytic active to dopamine (DA), but also displayed the constant peak current with the increase of concentration of DA. And a separated oxidation peak potential of about 270 mV between DA and Cu-THQ MOFs can be observed. Accordingly, a current ratio ( i DA /i MOFs ) was employed as readout signal for DA detection with a linear range and limit of detection of 3.0 × 10 −7 – 4.0 × 10 −5 mol/L and 2.6 × 10 −8 mol/L, respectively. Compared with single signal of i DA , ratiometric strategy exhibited high accuracy and reliability for DA determination. The present ratiometric sensing strategy using MOFs as the inner reference may provide a new choice for future development of electrochemical sensors for other analytes reliable determination.
研究领域:
分析化学
·
材料科学
·
生物医学
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产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2023
·
IF 4.1
Yixuan Fan,Runze Yu,Geoffrey I.N. Waterhouse,Ruiqiang Wang,Yufeng Sun,Zhixiang Xu
BACKGROUND Biogenic amines (BAs) in high concentrations are toxic and may cause a series of health symptoms. A sensitive measurement of BA levels is essential for human health. Capillary electrophoresis (CE) has emerged for the separation of eight BAs due to simple sample preparation and highly efficient separation. However, an important drawback for CE is low sensitivity. Magnetic solid-phase extraction (MSPE) has become a technique of interest owing to its brief operation and low solvent consumption. Hence, MSPE as a pretreatment has great potential to improve CE sensitivity for the analysis of BAs in complex food. RESULTS Results showed that the Pt-Co-MWCNTs-COOH possessed strong magnetism, good reusability, and high adsorptive ability toward eight biogenic amines based on the hydrogen bonding between the −COOH of Pt-Co-MWCNTs-COOH and −NH 2 groups of BAs. Using it as an adsorbent, a magnetic solid-phase extraction coupled with capillary electrophoresis (MSPE-CE) method was developed to effectively extract and sensitively analyze eight BAs. Under optimal conditions, the MSPE-CE method has wide linearities (10.0–1000.0 μg L −1 ) and low limits of detection (1.0–6.1 μg L −1 ). The accuracy of the developed method yielded recovery values from 82.07% to 102.58%. Meanwhile, the BAs contents in two samples were analyzed using the MSPE-CE method, with the results consistent with those detected by a high-performance liquid chromatography method. CONCLUSION Given those advantages, the established MSPE-CE method promises the practical guidance of monitoring a variety of BAs and provides a foundation for the detection of other food hazards. © 2023 Society of Chemical Industry.
研究领域:
分析化学
·
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jing Chen,Wenli Xue,Yaqin Shi,Wenjing Liu,Rongfang Ma
Catechin is a natural flavonoid with antioxidant, anticarcinogenic and anti-inflammatory properties. They can be used to treat or prevent human health problems. However, excessive intake of catechin can also be harmful to the human body. It is crucial to find a method for quantitative detection of catechin. The carboxyl-functionalized multi-walled carbon nanotubes were intercalated into the MXene layers to form a composite material with excellent electrical conductivity, and then MOF-808 with adsorption sites was further loaded on MXene/MWCNTs-COOH to synthesize MXene/MWCNTs-COOH/MOF-808 with excellent catalytic properties. Based on this, an electrochemical sensor was constructed for the detection of catechin. The CA sensor constructed in this work exhibited excellent analytical performance, good linear range (0.5–21.53 μM) and low detection limit (0.05 μM). It was also used for the detection of CA in green tea and beverages, which exhibited good recoveries (99.46–99.71 % and 96.46 % ~ 101.33 %) and the relative standard deviations (1.33–2.55 % and 0.07 % ~ 1.57 %).
✓ 已验证
产品关联
DOI
高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Shuting Shi,Chuanqin Zhou,Aiting Chen,Nana Tang,Kun Wan,Youwei Jiang,Jun Liu,Quanguo He,Li Cao
Antibiotics have emerged as a class of contaminants of concern globally, raising widespread worry and unease, primarily because of their inappropriately use and endless migration in food chains. Electrochemical ultrasensitive and selective determination of norfloxacin (NOR) using bismuth-based bimetallic salt bismuth molybdate (Bi 2 MoO 6 ) and carboxylated multi-walled carbon nanotube (MWCNTs-COOH) hybrid electrode as sensing platforms is presented hereby. The electrocatalytic ability of the electrodes was verified to be enhanced by the synergy effect of both Bi 2 MoO 6 and MWCNTs-COOH using cyclic voltammetry and electrochemical impedance spectroscopy. Linear scan voltammetry was applied to detect NOR. The oxidation peak current was linearly correlated with NOR concentration of 0.03–10 μM and the low limit detection of the optimized method was 6.7 nM (S/N = 3). In addition, it was verified that Bi 2 MoO 6 /MWCNTs-COOH/GCE probe had good stability, reproducibility, repeatability and selectivity. The spiked recovery method for NOR in milk and lake water samples showed recoveries of 94.8 – 96.9% and 90.2 – 98.3%, respectively, which can be used to determine NOR in real samples with high sensitivity. The preparation of Bi 2 MoO 6 /MWCNTs-COOH/GCE provides a new prototype for probing NOR detection at nanomolar concentration and safeguarding antibiotic contamination in environmental and food chains.
研究领域:
环境科学
·
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Na Liu,Youwei Jiang,Quanguo He,Tao Zhang,Yao Ping,Yelong Jiang,Wu Wang,Qijun Xie,Jianhua Ding
As an oral prodrug, valacyclovir undergoes rapid in vivo hydrolysis to form acyclovir - an agent with antiviral properties. This compound is extensively employed in addressing herpes virus infections and various other related conditions. Therefore, there is an urgent need to develop a method for detecting the content of valacyclovir in drugs. In this study, molybdenum disulfide (MoS 2 ) and carboxylated multi-walled carbon nanotubes (MWCNTs-COOH) were ultrasonically dispersed to prepare a suspension, which was drop-coated on the surface of a pretreated glassy carbon electrode (GCE). A MoS 2 /MWCNTs-COOH/GCE sensing platform was successfully constructed for the ultrasensitive detection of valacyclovir. Under optimized conditions, cyclic voltammetry (CV) was used to detect valacyclovir. A strong linear correlation between the oxidation peak current and the concentration was observed within the range of 0.1–40.0 μM, with a detection limit (LOD) of 0.013 μM. Meanwhile, the sensing platform also demonstrated excellent anti-interference capability, stability, and reproducibility. In the analysis of real samples, satisfactory recoveries for valacyclovir (96.5–104.0%) were achieved with a relative standard deviation (RSD) below 5.0%, further confirming its reliability. In summary, the developed MoS 2 /MWCNTs-COOH/GCE sensor offers a promising approach for the highly sensitive detection of valacyclovir.
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产品关联
DOI
高影响因子
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