Food Analytical Methods
·
2023
·
IF 2.9
Shen Jinhui,Liu Juan,Yang Siyi,Yao Xin,Fa Huanbao,Hou Changjun,Yang Mei
This work established a novel nucleic acid aptamer sensor for the sensitive and selective detection of AFB1 (Aflatoxin B1), which has important implication for food safety. In a word, we synthesized MXene/MWCNTs/NiCo 2 O 4 composite nanomaterials to improve the sensitivity of the aptamer sensor. The surface of the composite nanomaterials was modified with PDA (poly dopamine) in order to immobilize the NH 2 -cDNA-aptamer complex on the electrode surface by the Schiff base reaction. Characterization of MXene/MWCNTs/NiCo 2 O 4 nanocomposites was carried by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD). Meanwhile, 4-carboxy-2,2,6,6-tetramethylpiperidine 1-oxyl free radical (TEMPO-COOH) was used as an electrochemical signal to detect AFB1. Under optimal conditions, the electrochemical DPV signal decreased with increasing AFB1 concentration, ranging from 2.5 to 200 ng/mL, and the lowest limit of detection (LOD) at 1.890 ng/mL (S/N = 3). In addition, the ensemble sensor showed great reproducibility, stability, and selectivity, which has been successfully applied to the determination of AFB1 in maize flour and maize residue samples with recoveries in the 92.2 ~ 109.8% range. Compared to other detection methods for AFB1, this approach afforded comparable detection limits and detection ranges, in reduced analysis time (within 5 min). Thus, a time-saving, sensitive, and selective approach was achieved for the rapid determination of AFB1 in food.
研究领域:
食品安全
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分析化学
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生物传感技术
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Liang Tian,Mengyuan Jiang,Mengjie Su,Xiaoqing Cao,Qiyu Jiang,Quan Liu,Chunmei Yu
In this work, a highly selective and sensitive cortisol immunosensor based on MXene and multi-walled carbon nanotubes (MXene-MWCNTs) hybrid composite was proposed. The immunosensor was developed by screen printing technique on a flexible polyethyleneterephthalate (PET) substrate followed by modification of MXene-MWCNTs and anti-cortisol antibodies with EDC/NHS coupling agent. Owing to the large surface area and excellent conductivity of MXene-MWCNTs, the immunosensor exhibited a low detection limit and a wide linear range toward cortisol from 0.1 fg/mL to 1 µg/mL, which covered the normal range of free cortisol in sweat. Additionally, the immunosensor was applied to evaluate the cortisol level in human sweat samples, and the results were validated by a commercial chemiluminescence immunoassay (CLIA) assay. The present platform provides an effective method for non-invasive monitoring of human sweat cortisol levels, which may be well-suited for hormone screenings in healthy monitoring and medical diagnosis.
研究领域:
生物医学工程
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分析化学
·
医疗诊断
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jing Chen,Wenli Xue,Yaqin Shi,Wenjing Liu,Rongfang Ma
Catechin is a natural flavonoid with antioxidant, anticarcinogenic and anti-inflammatory properties. They can be used to treat or prevent human health problems. However, excessive intake of catechin can also be harmful to the human body. It is crucial to find a method for quantitative detection of catechin. The carboxyl-functionalized multi-walled carbon nanotubes were intercalated into the MXene layers to form a composite material with excellent electrical conductivity, and then MOF-808 with adsorption sites was further loaded on MXene/MWCNTs-COOH to synthesize MXene/MWCNTs-COOH/MOF-808 with excellent catalytic properties. Based on this, an electrochemical sensor was constructed for the detection of catechin. The CA sensor constructed in this work exhibited excellent analytical performance, good linear range (0.5–21.53 μM) and low detection limit (0.05 μM). It was also used for the detection of CA in green tea and beverages, which exhibited good recoveries (99.46–99.71 % and 96.46 % ~ 101.33 %) and the relative standard deviations (1.33–2.55 % and 0.07 % ~ 1.57 %).
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高影响因子
ELECTROANALYSIS
·
2021
·Vol. 33
·期 6
·1471-1483
·
IF 3.223
Shufang Ren,Runyan Feng,Shounian Cheng,Qingtao Wang,Zhixiang Zheng
Ti 3 C 2 T X MXene was synthesized by exfoliating pristine Ti 3 AlC 2 phase with hydrofluoric acid. The simple methods of mechanical mixing and drop-casting of Ti 3 C 2 T X and MWCNTs were carried out to prepare sensing electrode of Ti 3 C 2 T X /MWCNTs/GCE. The composite and topography, especially the surface functional groups of Ti 3 C 2 T X /MWCNTs were analyzed by XRD, SEM, FTIR, XPS, and Raman spectrum. The results turned out that Ti 3 C 2 T X was characteristic by accordion-like 2D nanostructure with the surfaces terminated with −OH, −F, and =O. When combining with acid pretreated, the interaction between the functional groups of Ti 3 C 2 T X and MWCNTs facilitated the convenience and reproducibility of the robust modified electrodes and could make Ti 3 C 2 T X /MWCNTs/GCE possess good synergistic catalytic acceleration by increasing the electron transfer efficiency as well as adsorption and aggregation of MOP analyte onto the electrode surface. Versatile electrochemical measurements of CV, DPV and EIS were used to investigate the electrochemical performance of Ti 3 C 2 T X /MWCNTs/GCE sensing platform. The linear detection range is 0.01–100 μM with the limit of detection of 0.0092 μM (S/N=3). The sensor has good stability, repeatability, reproducibility and anti-interference. In the detection of serum and urine samples, it has a good recovery rate.
研究领域:
材料科学
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分析化学
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医学检测
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DOI
高影响因子
ELECTROCHIMICA ACTA
·
2023
·
IF 6.6
Junqi Liu,Meng Wang,Saijin Wang,Yanli Zhao,Shi Zhou,Jinlian Li,Dongmei Wu
Oxygenated polycyclic aromatic hydrocarbons (OPAHs) present in the environment can enter the skin through direct contact and long-term exposure can increase the risk of skin inflammation, allergic reactions and skin cancer. In this study, a titanium dioxide-two-dimensional transition metal carbide/multi-walled carbon nanotubes/polydopamine/glassy carbon electrode (TiO 2 -MXene/MWCNTs/PDA/GCE) electrochemical biosensor was prepared to evaluate the cytotoxicity of OPAHs based on the changes in the content of xanthine/guanine (X/G) during cellular metabolism. The TiO 2 -MXene/MWCNTs/PDA/GCE exhibited excellent electrocatalytic activity and sensitivity. The surface morphology, structure and electrochemical properties of TiO 2 -MXene/MWCNTs/PDA composite material were characterized using scanning electron microscopy, X-ray diffraction and cyclic voltammetry. Human skin fibroblast cells (HFF-1) were used as model cells, and the cell viability was evaluated based on the electrochemical signal changes of X/G. When exposed to OPAHs for 30 h, the half-maximal inhibitory concentrations (IC 50 ) of 1,4-benzoquinone (1,4-BQ), 1,2-naphthoquinone (1,2-NQ), and 9,10-phenanthrenequinone (9,10-PQ) on HFF-1 cells were 291.88, 67.72, and 4.44 µmol·L −1 , respectively, which were lower than the IC 50 values of 1075.51, 113.96, and 9.63 µmol·L −1 obtained using the traditional MTT assay, indicating higher sensitivity of the sensor. This study achieved sensitive detection of purines in HFF-1 cells, providing a technical method for evaluating the cytotoxicity of persistent organic pollutants OPAHs in the environment.
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高影响因子
Journal of Materials Chemistry A
·
2023
·Vol. 12
·期 4
·2444-2455
·
IF 11.9
Zi-Fang Chen,Xingguang Chen,Chengwei Chen,Xiaoxu Lai,Jian Qin,Chi Chen,Dan Sun
Stretchable current collectors are essential components of deformable energy-storage devices. Deposition of a conductive layer on an elastomer surface is a common fabrication strategy for stretchable current collectors, however, facing the challenges of reducing the tensile resistance and film thickness. In this study, by integrating Ti3C2Tx nanosheets with MWCNTs, the authors deposited a staggered conductive network on the surface of an elastic styrene–ethylene–butylene–styrene (SEBS) film and thus created a stretchable hybrid conductor (TMS). The TMS current collector exhibits a low sheet resistance of about 31.3 Ω □−1 and shows relatively minor changes under stretching states. The current collector with a thickness of 144 μm possesses a strain ability of 100% and can recover to the original state when the stress is withdrawn. Besides, the TMS-based coin cell using V2O5 and LiMn2O4 as active materials achieves a reversible capacity of 85.5 mA h g−1 and delivers a high capacity retention of 95.4% (5C, 900 cycles), comparable to performance with the traditional rigid Ti current collectors (97.1%). Furthermore, the assembled coplanar batteries with a thickness of 366 μm can work under bending and stretching states with stable electrochemical performance. Moreover, the power supply tests under various working states demonstrated the ability of the stretchable LIBs to deliver stable power to a red LED, which shows a promising approach for developing thin and deformable energy-storage systems.
研究领域:
材料科学
·
能源存储技术
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高影响因子
SENSORS AND ACTUATORS A-PHYSICAL
·
2024
·
IF 4.1
Xiaoqing Cui,Jiahui Zheng,Yichen Huang,Rui Wang,Hongyan Zhang
Wearable pressure sensors with exceptional performance are playing a crucial role in various fields such as electronic skin, human motion monitoring, medical diagnosis, and human-computer interaction. However, achieving both high sensitivity and a wide sensing range with simple fabrication and low cost has proven to be a significant challenge for sensors. In this study, a simple capacitive pressure sensor is proposed that multi-walled carbon nanotubes (MWCNTs) are introduced into MXene (Ti 3 C 2 T X ) as a ‘bridge’. Then the electrode which has excellent conductivity is obtained by filtration of MXene solution and MXene/MWCNTs solution at intervals. Additionally, green and degradable cellulose paper is used as the dielectric layer. The fabricated pressure sensor exhibits a high sensitivity of 4.7 kPa −1 for low pressure from 0 to 1 kPa, a wide detection range of 0–700 kPa, ultra-fast response and recovery times of 46 ms and 62 ms, respectively, and an extremely low detection limit of 0.32 Pa. The sensor remains stable even after 4500 cycles and can accurately monitor the movement of the human joints. Furthermore, it can track human physiological signals, which is beneficial for medical diagnosis and disease prevention, and has significant potential for application in the wearable technology field.
研究领域:
材料科学
·
生物医学工程
·
电子皮肤技术
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DOI
高影响因子
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