CERAMICS INTERNATIONAL
·
2016
·
IF 2.758
Huan Zhou,Saisai Hou,Mingjie Zhang,Hong Chai,Yang Liu,Sarit B Bhaduri,Lei Yang,Linhong Deng
Biphasic calcium phosphate (BCP) compositions consisting of β-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) are potential biodegradable ceramics for bone regeneration. The present work demonstrates the formation of such dense ceramics by first preparing the precursors of nano-sized, amorphous , and equiaxed calcium pyrophosphate particles, and then sintering the precursors at 900 °C to transform them into desired BCP. However, if the complex of calcium tripolyphosphate was used, only CPP could be generated. It was also observed that the incorporation of Mg 2+ had several effects on the resulting products including: (1) promoting the generation of meso-porous precipitates; (2) favoring the formation of β-TCP instead of CPP; (3) reducing the grain size and increasing the density of the sintered ceramics, and (4) enhancing the negative electric charge of the BCP surface. Thus, the as-prepared BCP ceramics can serve as potential bone substitute materials in orthopedic applications.
研究领域:
材料科学
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生物医学工程
·
骨科
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DOI
高影响因子
JOURNAL OF MATERIALS ENGINEERING AND PERFORMANCE
·
2021
·Vol. 30
·期 10
·7214-7222
·
IF 1.819
Cai Hong,Li Xuan,Xue Feng,Chu Chenglin,Guo Chao,Bai Jing,Zhang Xiaobo
Abstract In order to ensure the safety of materials used in the field of orthopedic internal fixation, cytocompatibility was evaluated by culturing MC3T3-E1 cells with the extracts of Mg-2Zn rods, poly(lactic acid) (PLA) rods, hot pressed and hot drawn Mg wire/PLA composite rods. The adhesion, spread, and growth states of cells on each group of sample were analyzed according to SEM images. The fluorescent micromorphology was analyzed, and the relative growth ratio was calculated to quantitatively evaluate the influence of these extracts on the cell viability. Results displayed that moderate and severe cytotoxicity appeared after cells incubated with extracts of Mg-2Zn rod for 3 and 5 days, but it returned to normal phenomenon after the extracts diluted by 10 times. Extracts of other three groups of sample displayed good cytocompatibility. The growth and proliferation of cells were closely related to the pH value and concentration of Mg 2+ . The osmolality changes of extracts had little influence on cytocompatibility of samples at the early stage. Therefore, we can predict the effect of degradation products of Mg wire/PLA composites on their cytocompatibility during the degradation process, on the basis of pH value, concentration of Mg 2+ , as well as osmolality changes. The good cytocompatibility of Mg wire/PLA composites provided experimental valid and valuable guideline for further material design and scheme selection. Graphical Abstract
研究领域:
材料科学
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生物医学工程
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骨科
✓ 已验证
产品关联
DOI
高影响因子
Case Studies in Thermal Engineering
·
2023
·
IF 6.8
Huan Li,Xiaoyang Yu,Ke Qiu,Bing Bo,Qian Li,Shouxiang Lu
The active ingredients for aqueous film-forming foam (AFFF) are supplied as a concentrate, and the water used to dilute AFFF concentrates generally come from water sources around the fire site including seawater and river water. However, the effects of salt in water on fire-extinguishing performance of AFFF remain unclear. In this paper, we investigated the relationship between the interfacial properties, foam stability, and fire-extinguishing performance of three homemade AFFFs (nonionic AFFF APG , amphoteric AFFF CAB and anionic AFFF SDS ) and four commercial AFFFs. The seawater, firefighting water from different regions of China and deionized water were used to dilute AFFF concentrates. The results showed that AFFF APG and AFFF CAB had a better performance than AFFF SDS in resisting the negative effects of salts on interfacial properties and foaming ability. In the fire-extinguishing tests, we found that dilution of AFFF with water samples containing high concentrations of Ca 2+ and Mg 2+ would weaken the fire-extinguishing performance. The traditional indicators characterizing the properties of foam (surface tension, liquid drainage rate, and spreading coefficient) could not explain the trend of fire extinguishing performance. The stability of AFFF in the presence of oil could be used to explain the difference in fire-extinguishing time of AFFF.
研究领域:
火灾科学
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化学工程
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材料科学
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产品关联
DOI
高影响因子
Advanced Materials Technologies
·
2023
·
IF 6.8
Yang Song,Qiang Zhang,Yuxuan Li,Jiaxu Lin,Jin-Ming Lin
The loss of plasma membrane integrity poses a serious threat to cells, and the plasma membrane repair mechanism is vital to cell survival. To investigate the plasma membrane repair mechanism, a method for causing controllable plasma membrane injury on single cells and the visualization of the plasma membrane repair process are proposed. A microregion with harmful chemicals is created by open microfluidic probe, which is applied to target single cells for injury. The injury time is precisely controlled by simply moving the target cells in and out of the microregion. Wound-repair process caused by injury is analyzed by fluorescence imaging in real time, revealing the repair kinetics at the single cell level. Using this method, the effect of external conditions (Ca 2+ , Mg 2+ , glucose) on plasma membrane repair in single cells is studied and the dynamics of intracellular species (Ca 2+ ) is monitored. Meanwhile, cellular heterogeneity of plasma membrane repair is revealed. This method provides a precise way to induce chemical plasma membrane injury at the single-cell level, which is a promising tool to study the mechanism of plasma membrane repair.
研究领域:
细胞生物学
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生物物理学
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微流控技术
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产品关联
DOI
高影响因子
HYDROMETALLURGY
·
2023
·
IF 4.7
Changye Mang,Guanghui Li,Yanhu Chen,Jun Luo,Mingjun Rao,Tao Jiang
Partial neutralization is typically used to remove Fe 3+ ions from the acid leach solution of laterite. However, during this process, significant amounts of Mg 2+ , Ni 2+ , Co 2+ , and SO 2– 4 are lost. With increasing initial Fe 3+ concentration and solution pH, the severity of loss increases, with the precipitation of Fe 3+ . In this study, the mechanisms of ion co-precipitation during partial neutralization were investigated. Based on density functional theory calculations, a precipitation pathway through the formation of [Fe 4 (OH − ) 2 (SO 2– 4) 4 ]•[2SO 2 – 4 •yH 2 O]•[M 2+ ] was proposed. The differential charge density diagram indicated that the O atoms acted as the charge acceptors and maintained the precipitate structure. The coordination between M 2+ and SO 2– 4 could be destroyed via intense agitation, releasing approximately 100% of Mg 2+ , Ni 2+ , and Co 2+ ions from the precipitates to the solution. Furthermore, the addition of cationic or anionic surfactants limited the formation of these coordination bonds. The highest retention percentages of Mg 2+ , Ni 2+ , and Co 2+ in solution were 99.4, 99.6, and 99.9%, respectively, after adding 2 × 10 −5 mol/L of sodium dodecylbenzenesulfonate (CTAB). In addition, a high temperature could break these coordination bonds. The retention percentages of Mg 2+ , Ni 2+ , and Co 2+ increased from 82.6, 85.2, and 86.5% to 99.9, 99.6, and 99.8%, respectively, as the temperature increased from 25 to 65 °C. These results confirmed the rationality of the proposed structure of the precipitate.
研究领域:
化学工程
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材料科学
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环境化学
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产品关联
DOI
高影响因子
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
·
2023
·Vol. 39
·期 6
·1-13
·
IF 4.1
Zhu Di,Wu Caiyun,Niu Chenxin,Li Hong,Ge Fanglan,Li Wei
Corynebacterium glutamicum porphobilinogen synthase (PBGS) is a metal enzyme with a hybrid active site metal binding sequence. In this study, the porphobilinogen synthase gene of C. glutamicum was cloned and heterogeneously expressed in Escherichia coli. C. glutamicum PBGS was purified, and its enzymatic characteristics were analyzed. The results showed that C. glutamicum PBGS is a Zn 2+ -dependent enzyme, and Mg 2+ has allosteric regulation. The allosteric Mg 2+ plays a vital role in forming the quaternary structure of C. glutamicum PBGS. Based on the ab initio predictive structure modeling of the enzyme and the molecular docking model of 5-aminolevulinic acid (5-ALA), 11 sites were selected for site-directed mutagenesis. When the hybrid active site metal binding site of C. glutamicum PBGS is converted into a cysteine-rich motif (Zn 2+ -dependent) or an aspartic acid-rich motif (Mg 2+ /K + -dependent), the enzyme activity is basically lost. Four residues, D128, C130, D132, and C140, in the metal binding site, were the binding sites of Zn 2+ and the active center of the enzyme. The band migration, from the native PAGE, of five variants with mutations in the center of enzyme activity was the same as that of the variant enzymes as purified, individually adding two metal ion chelating agents. Their Zn 2+ active center structures were abnormal, and the quaternary structure equilibrium was altered. The destroyed active center affects the construction of its quaternary structure. The quaternary structural balance between octamer and hexamer through dimers was regulated by the allosteric regulation of C. glutamicum PBGS. The enzyme activity was also affected by the change of the active site lid structure and (α β) 8 -barrel structure caused by mutation. Structural changes in the variants were analyzed to understand C. glutamicum PBGS better.
研究领域:
生物化学
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分子生物学
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酶学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLoS One
·
2024
·Vol. 19
·期 9
·e0308424
·
IF 2.9
Yulin Liu,Longzhan Dong,Wenli Wu,Jiantao Ping,Jingbo Chen,Qiongzheng Hu
Hardness is one of the basic parameters of water, and a high-level hardness of drinking water may be harmful to human health. Thus, it is very important to monitor drinking water hardness. In this work, a portable lateral flow distance-based paper sensor for the semi-quantitative detection of drinking water hardness is demonstrated. In the presence of Ca 2+ /Mg 2+ , the hydrogel can be formed via the chelation between sodium alginate and Ca 2+ /Mg 2+ , inducing a phase separation process. The viscosity change of the sodium alginate solution is directly related to the Ca 2+ /Mg 2+ concentration and can be determined by the water lateral flow distance on test strips. The sensor successfully realizes the quantification of Ca 2+ and Mg 2+ in the range of 0–10 mmol L -1 and 4–20 mmol L -1 , respectively. The recoveries are found varied from 95% to 108.9%. The water hardness is acceptable for drinking if the C r values lies in the range of 0.259 to 0.419, and it is high with the C r value above 0.595. Remarkably, the performance of the sensor is comparable with the commercial kit for real water samples, which avoids the subjective judgment. Overall, this method provides a portable approach for semi-quantitative detection of drinking water hardness with the merits of convenience and low cost, which shows great potential for the potential application.
研究领域:
环境科学
·
分析化学
·
水质监测
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 8
·1-12
·
IF 5.3
He Qian,Chen Qun,Lian Lijin,Qu Jiuxin,Yuan Xi,Wang Chuhui,Xu Lidan,Wei Jiazhang,Zeng Shaoling,Yu Dongmei,Dong Yuhan,Zhang Yongbing,Deng Lin,Du Ke,Zhang Canyang,Pandey Vijay,Gul Ijaz,Qin Peiwu
The CRISPR/Cas13 nucleases have been widely documented for nucleic acid detection. Understanding the intricacies of CRISPR/Cas13’s reaction components is pivotal for harnessing its full potential for biosensing applications. Herein, we report on the influence of CRISPR/Cas13a reaction components on its trans-cleavage activity and the development of an on-chip total internal reflection fluorescence microscopy (TIRFM)-powered RNA sensing system. We used SARS-CoV-2 synthetic RNA and pseudovirus as a model system. Our results show that optimizing Mg 2+ concentration, reporter length, and crRNA combination significantly improves the detection sensitivity. Under optimized conditions, we detected 100 fM unamplified SARS-CoV-2 synthetic RNA using a microtiter plate reader. To further improve sensitivity and provide a new amplification-free RNA sensing toolbox, we developed a TIRFM-based amplification-free RNA sensing system. We were able to detect RNA down to 100 aM. Furthermore, the TIRM-based detection system developed in this study is 1000-fold more sensitive than the off-coverslip assay. The possible clinical applicability of the system was demonstrated by detecting SARS-CoV-2 pseudovirus RNA. Our proposed sensing system has the potential to detect any target RNA with slight modifications to the existing setup, providing a universal RNA detection platform. Graphical Abstract
CAS / MDL:
研究领域:
生物传感
·
分子生物学
·
纳米技术
·
病毒学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Li Liang,Xiaoqin Li,Zhang Lin,Chen Tian,Yiqing Guo
In this study, the microstructure of sulfidated nanoscale zero-valent iron (S-nZVI), and the reaction mechanism with cadmium (Cd) were investigated. Combining with spherical aberration-corrected scanning transmission electron microscope (Cs-STEM), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and Fourier transformed infrared (FTIR), it was found that the one-step synthesized S-nZVI with Na 2 S as the sulfidation reagent is irregular in shape and consisted of heterogeneous mixtures of Fe 0 , amorphous FeS, and Fe 3 O 4 . The dominant reaction mechanism between S-nZVI and Cd is displacement of Fe in FeS and the formation of CdS. The effects of Cd initial concentration, Mg 2+ , NO 3 − , and humic acid (HA) on the reaction kinetics were also studied. The removal capacity of Cd by S-nZVI can reach up to 495 mg/g, which is significantly higher than other S-nZVI and adsorbents. The reactions follow pseudo-second-order adsorption model, with chemisorption as the rate limiting step during the displacement reaction. Low concentration of Mg 2+ (<30 mg/L), NO 3 − (<60 mg/L), and HA (<10 mg/L) slightly decreases the removal of Cd due to the competition. Moreover, S-nZVI shows high efficiency and stability towards the removal of both free Cd ions and EDTA-chelated Cd during the concentration investigated. Overall, the study shows that sulfur plays a critical role in the particle microstructure and transformation of Cd in the environment.
研究领域:
环境科学
·
材料科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2018
·
IF 5.08
Yanze Bi,Xinyun Zhu,Jianliang Zhao,Hanrui Cui,Zhenguo Wang,Yan Li,Cai-qi Wang
Magnesium has a very promising adhibition in biomedical field for its excellent mechanical and biodegradable properties, however, the intelligent applications of biomedical magnesium developed difficultly due to its characteristic degradation. A intelligent biomedical magnesium was constructed on magnesium (Mg) surface by incorporating polydopamine (PD) and mechanized hollow mesoporous silica nanoparticles (HMSs) as smart delivery platform nanocontainers. The supramolecular nanovalves of mechanized HMSs consisted of alginate/chitosan multilayers by self-assembly, which are capable of entrapping rhodamine 6G in the mesopores and can release the cargo under the chemical environment of alkali or Mg iron stimuli that correspond to the degradation of biomedical Mg. The alkali/Mg 2+ dual stimuli-responsive release property of the HMSs endows the biodegradable Mg with controlled release potential. The well-designed smart delivery nanocontainers were combined with polydopamine deposited on Mg for excellent adhesion properties and positively charged amino group of PD. Furthermore, when the biomedical Mg with these mechanized HMSs was degraded in the simulated body environment, the alkali/Mg 2+ -triggered release of cargos from this smart delivery platform could bring a more functional application.
研究领域:
生物医学工程
·
材料科学
·
纳米技术
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2023
·Vol. 107
·期 5
·1725-1736
·
IF 5
Li Jing,Li Ziyi,Gong Hui,Ma Mengyi,Li Shuolei,Yang Huilin,Zhang Hailin,Liu Jianguo
Abstract In this study, a novel high-activity amylosucrase from Salinispirillum sp. LH10-3-1 (SaAS) was identified and characterized. The recombinant enzyme was determined as a monomer with a molecular mass of 75 kDa. SaAS protein exhibited the maximum total and polymerization activities at pH 9.0 and maximum hydrolysis activity at pH 8.0. The optimum temperature for total, polymerization, and hydrolysis activities were 40, 40, and 45 °C, respectively. Under the optimal pH and temperature, SaAS had a specific activity of 108.2 U/mg. SaAS also showed excellent salt tolerance and could retain 77.4% of its original total activity at 4.0 M NaCl. The addition of Mg 2+ , Ba 2+ , and Ca 2+ enhanced the total activity of SaAS. When the conversion of 0.1 M and 1.0 M sucrose was catalyzed at pH 9.0 and 40 °C for 24 h, the ratios of hydrolysis, polymerization, and isomerization reactions were 11.9:77.4:10.7 and 15.3:53.5:31.2, respectively. The α-arbutin yield of 60.3% was achieved from 20 mM sucrose and 5 mM hydroquinone catalyzed by SaAS. Key points
• A novel amylosucrase from Salinispirillum sp. LH10-3-1 (SaAS) was characterized.
• SaAS has the highest specific enzyme activity among all known amylosucrase.
• SaAS has hydrolysis, polymerization, isomerization, and glucosyltransferase activities.
Graphical Abstract
研究领域:
生物化学
·
酶学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AIP Advances
·
2022
·Vol. 12
·期 5
·
IF 1.697
Tu Huan,Zhao Guoying,Hou Jingshan,Liu Yufeng,Zhou Yan,Zhang Ganghua,Sun Hong-Tao,Li Ji-Guang,Fang Yongzheng
White light emitting diodes (LEDs) suffer from two most common drawbacks: the deficiency of the red component and the poor thermal stability of silicone. Ce 3+ -doped garnet phosphors with tailor-made luminescence properties can be achieved by variations in {A}, {B}, and {C} cation sites. Herein, a simultaneous ion co-substitution design strategy has been adopted to synthesize a series of (Y, Ca) 3 (Al, Mg) 2 (Al, Si) 3 O 12 garnet phosphors and corresponding phosphor-in-glass (PiG) films. The co-substitution is finely restricted to the assigned sites. The emission peak is shifted from 529 to 565 nm, which is beneficial to obtain low color temperatures and high color rendering using a single phosphor for white LEDs. By employing the as-synthesized phosphor, the corresponding PiG films were fabricated by the low temperature co-sintering technology using the SiO 2 –B 2 O 3 –ZnO–Na 2 O glass system characterized with excellent thermal stability and moisture resistance. The morphological and elemental analyses demonstrated that the as-made phosphor powders were uniformly distributed in the glass host without any interfacial reactions. Finally, modular white LEDs with a high color rendering (Ra = 82.7) is achieved through the as-synthesized PiG film on an InGaN blue chip. This study may open up a facile approach to obtain high quality luminescence based on the mono-luminescence center, avoiding excitation energy wastage and low quantum efficiency aroused by multi-luminescence centers or color compensation phosphors in glass.
研究领域:
材料科学
·
光电子学
·
固态照明
·
无机化学
·
应用物理学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）